PITX1, a specificity determinant in the HIF-1α-mediated transcriptional response to hypoxia

Hypoxia is an important developmental cue for multicellular organisms but it is also a contributing factor for several human pathologies, such as stroke, cardiovascular diseases and cancer. In cells, hypoxia activates a major transcriptional program coordinated by the Hypoxia Inducible Factor (HIF) family. HIF can activate more than one hundred targets but not all of them are activated at the same time, and there is considerable cell type variability. In this report we identified the paired-like homeodomain pituitary transcription factor (PITX1), as a transcription factor that helps promote specificity in HIF-1α dependent target gene activation. Mechanistically, PITX1 associates with HIF-1β and it is important for the induction of certain HIF-1 dependent genes but not all. In particular, PITX1 controls the HIF-1α-dependent expression of the histone demethylases; JMJD2B, JMJD2A, JMJD2C and JMJD1B. Functionally, PITX1 is required for the survival and proliferation responses in hypoxia, as PITX1 depleted cells have higher levels of apoptotic markers and reduced proliferation. Overall, our study identified PITX1 as a key specificity factor in HIF-1α dependent responses, suggesting PITX1 as a protein to target in hypoxic cancers

HIV-2 viral production and infectivity are affected by APO3 host factors

Poster presented at the 7th Postgraduate iMed.ULisboa Students Meeting. Faculty of Pharmacy, Universidade de Lisboa, 15-16 July 2015.Egas Moniz - Cooperativa de Ensino Superior CRL and Fundação para a Ciência e Tecnologia, Lisbon, Portuga

APOBEC3 host factors modulate viral production and infectivity of HIV-2

Poster presented at the 15th European AIDS Conference/EACS. Barcelona, 21-24 October 2015.This work was funded by FCT – SFRH/BD/81921/2011 and Egas Moniz, Cooperativa de Ensino Superior, CRL, Portugal

Cytotoxic and genotoxic effects of three cements used in fixed prosthesis

Poster presented at the First international Congress of CiiEM - From Basic Sciences to Clinical Research. Egas MOniz, Caparica, Portugal, 27-28 November 2015Egas Moniz - Cooperativa de Ensino Superior CR

Cytotoxicity comparison of 2 bulk fill resin-composites

Poster presented at the CED-IADR/NOF Oral Health Research Congress (2017 Continental European and Scandinavian Divisions Meeting). September 21-23, 2017, Vienna, Austria.N/

Effects of three current provisional restoration materials on fibroblast viability

Poster presented at the First international Congress of CiiEM - From Basic Sciences to Clinical Research. Egas Moniz, Caparica, Portugal, 27-28 November 2015Egas Moniz Cooperativa de Ensino Superior CR

Mercury in vitro bioaccessibility in seaweed (Ulva sp.): effects of cooking practices.

SPASS 2016