Poster presented at the CED-IADR/NOF Oral Health Research Congress (2017 Continental European and Scandinavian Divisions Meeting). September 21-23, 2017, Vienna, Austria.N/

Azul, A. M.

Bandarra, S.

Barahona, I.

Mascarenhas, P.

Proença, L.

Ribeiro, A. Ribeiro

Simões, S.A.

Journal of Dental Research

English

Repositório Comum

CED-/NOF    —   ORAL HEALTH RESEARCH CONGRESS    —   IADR  2017       The use of composite resins as restorative materials in Dentistry has been extensive. However, the toxicity has been linked to some of its components, in particular the monomers present as a result of incomplete polymerization, which may elute into the oral cavity1,2. The appearance on the market of a new range of resins that calls the possibility of restoration block with thicknesses up to 4 mm requires further studies in vitro and in vivo to ensure its biocompatibility.    To compare the cytotoxic reaction produced by two different composite resins Bulk fill, namely Filtek™ Bulk Fill (3M™ ESPE) (FBF) and Tetric EvoCeram® Bulk Fill (Ivoclar Vivadent®) (TEC), used in dental restoration, in cultures of fibroblasts in order to infer about their biocompatibility. S.A. Simões, S. Bandarra, L. Proença, P. Mascarenhas, A. Ribeiro, A. M. Azul, I. Barahona  Centro de Investigação Interdisciplinar Egas Moniz (CiiEM), Instituto Superior de Ciências da Saúde Egas Moniz (ISCSEM), Monte da Caparica, Portugal Cytotoxicity Comparison of 2 Bulk Fill Resin-Composites 0046 1. Introduction 2. Objective                    In the first 24h, there was release of cytotoxic components in both resins.  They were cytotoxic in all dilutions tested, although no significant differences were observed between them.   The identification of toxic components is important to avoid their use in dentistry. 3. Methods 4. Results Fig. 2 — TEC and FBF resin-composites5,6 used in this study (left); The 96-well plate after incubation of the cells for 24 h with the resin extracts and solubilization of the formazan crystals with dimethyl sulfoxide (DMSO), immediately prior to the spectrophotometric analysis (center); Spectrophotometer microplate reader, model 680  (Bio Rad®) (center).  Fig. 1 — 3T3 fibroblasts  observed at the microscope: 1 day after thawing (left); With 80% confluence after 3 days of incubation in DMEM - used in cytotoxicity tests (center); Morphological changes visible after 24h of contact with FBF100% (right).  5. Conclusions Cell viability data were statistically analysed by using a two-way ANOVA, at a significance level of 5%, considering resin and extract concentration as main effects. Citotoxicity (%) Concentration of the extract  TEC FBF 100% 72,0 81,1 50% 49,2 39,6 10% 41,5 36,9  Table 1 - Comparative cytotoxicity between the TEC and FBF resins at different dilutions of extract.    The authors gratefully acknowledge 3M™ ESPE and Ivoclar Vivadent® for supplying the resins used in this study.  This study was supported by Egas Moniz — Cooperativa de Ensino Superior CRL. Acknowledgements  1) Geurtsen, W. (2000). Biocompatibility of Resin-Modified Filling Materials. Critical Reviews in Oral Biology & Medicine, 11(3), 333–355.   2) Van Landuyt, K. L., et al. (2011). How much do resin-based dental materials release? A meta-analytical approach. Dental Materials, 27(8), 723–747.  3) International Organization for Standardization. (2008). Evaluation of biocompatibility of medical devices used in dentistry.  4) International Organization for Standardization. (2009). Biological evaluation of medical devices. Part 5: In vitro cytotoxicity tests (ISO 10993-5: 2009)  5) 3M ESPE. (2015). Filtek TM Bulk Fill - Scientific Documentation. 6) Ivoclar Vivadent (2016). Tetric EvoCeram Bulk Fill, Scientific Documentation. Instructions for use  7) Vitral, J. C. D. A. (2008). Avaliação da Citotoxidade de Materiais Odontológicos Através do Método de MTT e Produção de Óxido Nítrico: Descrição de uma Técnica. Pesquisa Brasileira Em Odontopediatria E Clínica Integrada, 8(3), 359–365.   Bibliography 

Cytotoxicity comparison of 2 bulk fill resin-composites

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Cytotoxicity comparison of 2 bulk fill resin-composites

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