Development and validation of an automatic thermal imaging process forassessing plant water status

[EN] Leaf temperature is a physiological trait that can be used for monitoring plant water status. Nowadays, by means of thermography, canopy temperature can be remotely determined. In this sense, it is crucial to automatically process the images. In the present work, a methodology for the automatic analysis of frontal images taken on individual trees was developed. The procedure can be used when cameras take at the same time thermal and visible scenes, so it is not necessary to reference the images. In this way, during the processing in batch, no operator participated. The procedure was developed by means of a non supervised classification of the visible image from which the presence of sky and soil could be detected. In case of existence, a mask was performed for the extraction of intermediate pixels to calculate canopy temperature by means of the thermal image. At the same time, sunlit and shady leaves could be detected and isolated. Thus, the procedure allowed to separately determine canopy temperature either of the more exposed part of the canopy or of the shaded portion. The methodology developed was validated using images taken in several regulated deficit irrigation trials in Persimmon and two citrus cultivars (Clementina de Nules and Navel Lane-Late). Overall, results indicated that similar canopy temperatures were calculated either by means of the automatic process or the manual procedure. The procedure developed allows to drastically reduce the time needed for image analysis also considering that no operator participation was required. This tool will facilitate further investigations in course for assessing the feasibility of thermography for detecting plant water status in woody perennial crops with discontinuous canopies. Preliminary results reported indicate that the type of crop evaluated has an important influence in the results obtained from thermographic imagery. Thus, in Persimmon trees there were good correlations between canopy temperature and plant water status while, in Clementina de Nules and Navel Lane-Late citrus cultivars canopy temperature differences among trees could not be related with tree-to-tree variations in plant water status.This research was supported by funds from the Instituto Valenciano de Investigaciones Agrarias and the "Denominacion de origen Caqui Ribera del Xuquer" via "Proyecto Integral Caqui". from projects Rideco-Consolider CSD2006-0067 and Interreg IV Sudoe Telerieg. Thanks are also due to J. Castel, E. Badal, I. Buesa and D. Guerra for assistance with field work and to the Servicio de Tecnologia del Riego for providing the meteorological data.Jiménez Bello, MÁ.; Ballester, C.; Castel Sanchez, R.; Intrigliolo Molina, DS. (2011). Development and validation of an automatic thermal imaging process forassessing plant water status. Agricultural Water Management. (98):1497-1504. https://doi.org/10.1016/j.agwat.2011.05.002S149715049

Thermographic measurement of canopy temperature is a useful tool for predicting water deficit effects on fruit weight in citrus trees

[EN] Alternative and more practical methods for plant water stress detection than stem water potential (ψs) and stomatal conductance (gs) are needed when regulated deficit irrigation (RDI) strategies are applied. The aim of this experiment was to compare sap flow and canopy temperature (Tc) measurements with more classical methods like ψs or gs to predict the effect of deficit irrigation on fresh fruit weight in citrus trees. The experiment was performed during the summer of 2011 in a “Clementina de Nules” orchard undergoing RDI. Sap flow was determined by means of the compensation heat pulse method in well-watered and RDI trees. Tc was measured continuously with infrared thermometers (IRTs) mounted over the canopies and also weekly with an infrared hand-operated thermographic camera taking frontal images of the sunlit side of tree crowns. Concurrently, ψs and gs were also measured on all trees. Results showed that the evolution of the relative transpiration obtained with the sap flow sensors was in agreement with the plant water stress experienced. The values of Tc obtained with the fixed IRTs, normalized by air temperature (Tc − Ta) were in general poorly related with ψs and gs. However, when Tc was obtained from thermal imaging, there was a good correlation with ψs in days of relatively high water stress (i.e. when ψs differences among treatments were >1.0 MPa). The average fruit weight at harvest was significantly correlated with all the stress indicators, and the best correlation was that of thermographic Tc followed by ψs and gs. Overall, results showed that in citrus trees Tc measurement obtained from thermal imaging is a good tool to predict the effect of water deficit on fresh fruit weight.The authors thank H. Hernandez and T. Yeves for their help with the field work. This experiment has been funded by the RIDECO-CONSOLIDER CSD2006-00067 and Telerieg Interreg IV Sudoe projects. C. Ballester was holder of a predoctoral fellowship FPI-INIA-CCAA. D.S. Intrigliolo acknowledges the financial support received from the Spanish Ministry of Economy and Competitiveness (MINECO) program "Ramon y Cajal".Ballester, C.; Castel, J.; Jiménez Bello, MÁ.; Castel Sánchez, JR.; Intrigliolo Molina, DS. (2013). Thermographic measurement of canopy temperature is a useful tool for predicting water deficit effects on fruit weight in citrus trees. Agricultural Water Management. 122:1-6. https://doi.org/10.1016/j.agwat.2013.02.005S1612

Usefulness of thermography for plant water stress detection in citrus and persimmon trees

[EN] The feasibility of using canopy temperature (Tc) measured with a hand-operated infrared thermographic camera as a water stress indicator was evaluated in the field during two seasons on citrus and persimmon trees subjected to different levels of deficit irrigation. In both species, which differ in leaf anatomy and stomatal response to environmental conditions, Tc was compared with midday stem water potential (s) measurements. In persimmon trees, leaf stomatal conductance (gs) was also measured. In 2009, images were taken from the sunlit and shady sides of the canopies. Based on the results obtained, during the second experimental season images were taken from the sunlit side of the trees and also from above the canopy. In persimmon, trees under deficit irrigation had lower s and gs what resulted in a clear increase in Tc regardless of the position from where the pictures were taken. The maximum Tc difference between deficit-irrigated and control trees observed was of 4.4 ◦C, which occurred when the stressed trees hads values 1.1 MPa lower than the control ones. In persimmon trees, Tc was the most sensitive indicator of plant water status particularly due to the lower tree-to-tree variability as compared to s and gs. On the other hand, in citrus trees Tc was not always affected by plant water stress. Only in the second experimental season, when air vapour pressure deficit values were below 2.7 kPa and images were also taken from above the canopies, deficit-irrigated trees had higher Tc than the control ones, this difference being at most 1.7 ◦C. Overall, the results show that hand-operated thermographic cameras can be used to detect plant water stress in both fruit tree species. Nevertheless, the use of Tc measurements to detect plant water stress appears to be more precise in persimmon than in orange citrus. This might be because persimmon trees have larger leaf size which determines higher canopy resistance allowing for higher increases in canopy temperature in response to water stress via stomatal closure. © 2012 Elsevier B.V. All rights reserved.The authors thank E. Badal, I. Buesa, J. Castel, D. Guerra, D. Perez, F. Sanz and A. Yeves, for their help in field work. This research was supported by funds from projects RIDECO-CONSOLIDER CSD2006-00067, Telerieg Interreg IV Sudoe and "Proyecto Integral Caqui". D.S. Intrigliolo acknowledges the financial support received from the Spanish Ministry of Economy and Competitiveness (MINECO) program "Ramon y Cajal".Ballester, C.; Jiménez Bello, MÁ.; Castel, JR.; Intrigliolo, DS. (2013). Usefulness of thermography for plant water stress detection in citrus and persimmon trees. Agricultural and Forest Meteorology. 168:120-129. https://doi.org/10.1016/j.agrformet.2012.08.005S12012916

Autoantibodies against type I IFNs in patients with life-threatening COVID-19

Interindividual clinical variability in the course of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection is vast. We report that at least 101 of 987 patients with life-threatening coronavirus disease 2019 (COVID-19) pneumonia had neutralizing immunoglobulin G (IgG) autoantibodies (auto-Abs) against interferon-w (IFN-w) (13 patients), against the 13 types of IFN-a (36), or against both (52) at the onset of critical disease; a few also had auto-Abs against the other three type I IFNs. The auto-Abs neutralize the ability of the corresponding type I IFNs to block SARS-CoV-2 infection in vitro. These auto-Abs were not found in 663 individuals with asymptomatic or mild SARS-CoV-2 infection and were present in only 4 of 1227 healthy individuals. Patients with auto-Abs were aged 25 to 87 years and 95 of the 101 were men. A B cell autoimmune phenocopy of inborn errors of type I IFN immunity accounts for life-threatening COVID-19 pneumonia in at least 2.6% of women and 12.5% of men