Entre restos, há quem sonhe : sobre a escuta psicanalítica com pessoas em situação de rua

Esta pesquisa surgiu a partir de indagações sobre as possibilidades da escuta psicanalítica na Política Pública de Assistência Social, mais especificamente, em um serviço dedicado ao trabalho com pessoas em situação de rua no município de Porto Alegre/RS. Iniciou-se com considerações sobre lugares que pessoas ocupam na malha social, lugares pré-determinados por um discurso que dá coordenadas para constituições ou destituições subjetivas. No contexto da pobreza, essas pessoas tomadas como um “resto” da maquinaria capitalista, muitas vezes, não encontram endereçamentos possíveis de fala. Com a psicanálise resgatamos o que há de mais fundamental nesse campo: a função enunciativa desses sujeitos. Por ser uma experiência singular, sua metodologia de escrita aconteceu a partir dos efeitos que essa escuta produziu no trabalhador: sonhar. É com os sonhos, elemento que Freud destacou como via privilegiada ao inconsciente, do lado de quem escuta, que apontamos formas de fazer furos aos discursos totalitários e políticas da indiferença. Assim, se com os “restos” trabalhamos, foi a partir do que resta dessa clínica que se criou possibilidades de transmissão.The present study was originated from the author’s questions about the possibility of psychoanalytic listening with individuals attended by Brazilian public policy of Social Assistance, more specifically by a municipal service directed to homeless people at Porto Alegre, Rio Grande do Sul. It was started with considerations about those individual’s social categorization, which is predetermined by a discourse that leads to subjective formations and destitutions. In the poverty context, those people are taken by the capitalist machine as a “leftover”, usually neglected of possible speech position. Through psychoanalysis, it’s possible to retrieve the most fundamental thing there is on that context: their enunciative function. Since it’s a singular experience, the study writing method developed from the effect that the approach brought to the researcher: to dream. By dreaming, element that Freud highlighted as unconscious Via Regia, the listening one indicates ways of breaking the totalitarian discourse and the actions of social indifference. Therefore, working with the “leftovers”, transmission possibilities were created with what remained from this clinic

Situação De Rua e Abordagem Social: Desafios Operacionais ao SUAS

Uma vez revelada a complexidade das experiências biográficas de pessoas em situação de rua, é urgente o aumento do número de estudos que qualifiquem a relação prática entre usuárias/os, políticas públicas e a representação do Estado na execução dos serviços de proteção. Para esta discussão, adicionamos as idiossincrasias sócio-históricas acerca do fenômeno da situação de rua no Brasil e a sua relação com o funcionamento do Sistema Único de Assistência Social (SUAS). No intuito da expansão teórico-prática dessa problemática, este estudo objetivou relatar as experiências de trabalho psicossocial dentro do Estágio Profissional de Psicologia em um serviço de abordagem social no município de Porto Alegre, Brasil. Nossos resultados alocam alguns aspectos estruturais, sócio-históricos e biopolíticos como elementos que interferem na execução ótima dos serviços tipificados pelas políticas públicas, marcando a dissonância entre políticas e suas implementações nos serviços

Experimental Infection by Brucella ovis: Changes in NTPDase, 5'-Nucleotidase and Acetylcholinesterase Associated Cerebral Oxidative Stres

Background: Changes in purinergic and cholinergic signaling have been demonstrated in various pathologies associated with inflammation; however, the changes in brucellosis caused by the Gram-negative coccobacillus Brucella ovis are not known. B. ovis is generally asymptomatic in sheep. Hepatosplenomegaly has been described in B. ovis, a non-zoonotic species, characterized by an extravascular inflammatory response. Purinergic system enzymes are closely involved with the modulation of the immune system, pro- and anti-inflammatory events. The objective of this study was to investigate the role of ectonucleotidases and cholinesterase’s in the brains of mice experimentally infected with B. ovis.Materials, Methods & Results: Forty-eight animals were divided into two groups: control (n = 24) and infected (n = 24). In group infected, 100 µL containing 1.3 x 107 UFC B. ovis /mL via intraperitoneal was used in inoculation. The brains were collected from the animals on days 7, 15, 30 and 60 post-infection (PI). We measured levels of TBARS (substances reactive to thiobarbituric acid) and ROS (reactive oxygen species) in the brain. The activity of NTPDase (using ATP and ADP as substrate) and 5'-nucleotidase (using AMP as substrate) were evaluated in brain in addition to histopathological analysis. No histopathological lesions were observed in the control group nor the infected group at days 7, 15, and 30 PI. However,multifocal areas with moderate microgliosis and inflammatory infiltrates in the cerebral cortex were observed at day 60 PI in the infected animals. B. ovis DNA was detected in brain. During the course of infection, B. ovis caused greater lipid peroxidation in the brains of infected animals than in the control group at day 60PI. No significant results were observed at 7, 15 or day 30 PI. Similarly, there was significantly more reactive oxygen species at day 60 PI in brains of infected animals than in the control group. NTPDase activity (using ATP and AMP as substrate) was lower at days 7 and 15 PI in infected animals than in control. However, during the course of infection there was an increase in NTPDase activity at day 60 PI in the infected group. The infected animals showed a decrease of 5´-nucleotidase (AMP as substrate) activity at days 7 and 30 PI. On the other hand, 5´-nucleotidase activity was greater on day 60 PI in the experimental group than in the control. The results suggest that nucleotide hydrolysis was low in the acute phase (up to day 30 PI) due to the decrease of NTPDase and 5´-nucleotidase activities. After day 60 PI, there was a reversal in enzyme activities, probably with concomitant increase of extracellular nucleotides. AChE activity in brain on days 30 and 60 PI compared to control.Discussion: Among the functions of NTPDase are inhibition of platelet aggregation, vascular homeostasis, modulation of inflammation and immune response, all via its regulation of extracellular concentrations of ATP, a pro-inflammatory molecule. E-NTPDase plays an important role in controlling lymphocyte function, including antigen recognition and activation of cytotoxic T cell effector functions, as well as the generation of signals. The enzyme E-5´-nucleotidase also exerts non-enzymatic functions, including induction of intracellular signaling and mediation of cell-cell adhesion and cell-matrix and migration. Levels of acetylcholine are regulated by cholinesterase enzymes that are present in cholinergic and noncholinergic tissues, as the acetylcholinesterase (AChE) is a membrane-bound enzyme, primarily found in the brain and cholinergic neurons, where it participates in the structural regulation of postsynaptic differentiation. The results demonstrated that the chronicity of infection by B. ovis causes oxidative damage and inflammation in the brain, as well as modulation of ectonucleotidases and AChE activities

Habitantes de la calle en tiempos de pandemia

Objetivo: Este artigo visa relatar as experiências de abordagem interprofissional à população em situação de rua durante o curso da pandemia de Covid-19 em uma Unidade Básica de Saúde do município de Porto Alegre, Rio Grande do Sul, Brasil. Método: Trata-se de um estudo na modalidade de relato de experiência com base na observação e participação da equipe multiprofissional da unidade de saúde articulado com o serviço especializado em abordagem social da região de atendimento, no período de abril a julho de 2020, correspondente ao curso da pandemia de Covid-19 no Brasil. Resultados: Os resultados foram relatados em dois eixos: a formação de um grupo de trabalho interprofissional e intersetorial e a dimensão social da assistência em saúde à população de rua. Conclusão: Considera-se que a implantação de um plano específico de cuidados à população em situação de rua no âmbito da Atenção Primária à Saúde evidenciou, de um lado, as iniquidades e as fragilidades das ações de proteção a este segmento populacional, e de outro mostrou a potencialidade das ações intersetoriais no território

Entre restos, há quem sonhe : sobre a escuta psicanalítica com pessoas em situação de rua

Esta pesquisa surgiu a partir de indagações sobre as possibilidades da escuta psicanalítica na Política Pública de Assistência Social, mais especificamente, em um serviço dedicado ao trabalho com pessoas em situação de rua no município de Porto Alegre/RS. Iniciou-se com considerações sobre lugares que pessoas ocupam na malha social, lugares pré-determinados por um discurso que dá coordenadas para constituições ou destituições subjetivas. No contexto da pobreza, essas pessoas tomadas como um “resto” da maquinaria capitalista, muitas vezes, não encontram endereçamentos possíveis de fala. Com a psicanálise resgatamos o que há de mais fundamental nesse campo: a função enunciativa desses sujeitos. Por ser uma experiência singular, sua metodologia de escrita aconteceu a partir dos efeitos que essa escuta produziu no trabalhador: sonhar. É com os sonhos, elemento que Freud destacou como via privilegiada ao inconsciente, do lado de quem escuta, que apontamos formas de fazer furos aos discursos totalitários e políticas da indiferença. Assim, se com os “restos” trabalhamos, foi a partir do que resta dessa clínica que se criou possibilidades de transmissão.The present study was originated from the author’s questions about the possibility of psychoanalytic listening with individuals attended by Brazilian public policy of Social Assistance, more specifically by a municipal service directed to homeless people at Porto Alegre, Rio Grande do Sul. It was started with considerations about those individual’s social categorization, which is predetermined by a discourse that leads to subjective formations and destitutions. In the poverty context, those people are taken by the capitalist machine as a “leftover”, usually neglected of possible speech position. Through psychoanalysis, it’s possible to retrieve the most fundamental thing there is on that context: their enunciative function. Since it’s a singular experience, the study writing method developed from the effect that the approach brought to the researcher: to dream. By dreaming, element that Freud highlighted as unconscious Via Regia, the listening one indicates ways of breaking the totalitarian discourse and the actions of social indifference. Therefore, working with the “leftovers”, transmission possibilities were created with what remained from this clinic

Experimental Infection by Brucella ovis: Changes in NTPDase, 5'-Nucleotidase and Acetylcholinesterase Associated Cerebral Oxidative Stres

Background: Changes in purinergic and cholinergic signaling have been demonstrated in various pathologies associated with inflammation; however, the changes in brucellosis caused by the Gram-negative coccobacillus Brucella ovis are not known. B. ovis is generally asymptomatic in sheep. Hepatosplenomegaly has been described in B. ovis, a non-zoonotic species, characterized by an extravascular inflammatory response. Purinergic system enzymes are closely involved with the modulation of the immune system, pro- and anti-inflammatory events. The objective of this study was to investigate the role of ectonucleotidases and cholinesterase’s in the brains of mice experimentally infected with B. ovis.Materials, Methods & Results: Forty-eight animals were divided into two groups: control (n = 24) and infected (n = 24). In group infected, 100 µL containing 1.3 x 107 UFC B. ovis /mL via intraperitoneal was used in inoculation. The brains were collected from the animals on days 7, 15, 30 and 60 post-infection (PI). We measured levels of TBARS (substances reactive to thiobarbituric acid) and ROS (reactive oxygen species) in the brain. The activity of NTPDase (using ATP and ADP as substrate) and 5'-nucleotidase (using AMP as substrate) were evaluated in brain in addition to histopathological analysis. No histopathological lesions were observed in the control group nor the infected group at days 7, 15, and 30 PI. However,multifocal areas with moderate microgliosis and inflammatory infiltrates in the cerebral cortex were observed at day 60 PI in the infected animals. B. ovis DNA was detected in brain. During the course of infection, B. ovis caused greater lipid peroxidation in the brains of infected animals than in the control group at day 60PI. No significant results were observed at 7, 15 or day 30 PI. Similarly, there was significantly more reactive oxygen species at day 60 PI in brains of infected animals than in the control group. NTPDase activity (using ATP and AMP as substrate) was lower at days 7 and 15 PI in infected animals than in control. However, during the course of infection there was an increase in NTPDase activity at day 60 PI in the infected group. The infected animals showed a decrease of 5´-nucleotidase (AMP as substrate) activity at days 7 and 30 PI. On the other hand, 5´-nucleotidase activity was greater on day 60 PI in the experimental group than in the control. The results suggest that nucleotide hydrolysis was low in the acute phase (up to day 30 PI) due to the decrease of NTPDase and 5´-nucleotidase activities. After day 60 PI, there was a reversal in enzyme activities, probably with concomitant increase of extracellular nucleotides. AChE activity in brain on days 30 and 60 PI compared to control.Discussion: Among the functions of NTPDase are inhibition of platelet aggregation, vascular homeostasis, modulation of inflammation and immune response, all via its regulation of extracellular concentrations of ATP, a pro-inflammatory molecule. E-NTPDase plays an important role in controlling lymphocyte function, including antigen recognition and activation of cytotoxic T cell effector functions, as well as the generation of signals. The enzyme E-5´-nucleotidase also exerts non-enzymatic functions, including induction of intracellular signaling and mediation of cell-cell adhesion and cell-matrix and migration. Levels of acetylcholine are regulated by cholinesterase enzymes that are present in cholinergic and noncholinergic tissues, as the acetylcholinesterase (AChE) is a membrane-bound enzyme, primarily found in the brain and cholinergic neurons, where it participates in the structural regulation of postsynaptic differentiation. The results demonstrated that the chronicity of infection by B. ovis causes oxidative damage and inflammation in the brain, as well as modulation of ectonucleotidases and AChE activities