Correlation Between Quadriceps and Hamstring Isokinetic Strength to Ball Velocity During a Soccer Kick

When kicking a soccer ball, large forces are generated by the quadriceps and hamstring muscles that extend and flex the knee. The angular acceleration[GJR1] at this joint and the torques produced are[GJR2] related. PURPOSE: The goal of this pilot study was to explore the relationship between isokinetic strength[GJR3] of the quadriceps and hamstring muscles to velocity of a kicked soccer ball and determine if isokinetic testing of quadriceps and hamstring strength can predict soccer ball velocity during a kick. Methods: Four female NCAA Division II soccer athletes completed maximal effort knee flexion and extension at three isokinetic speeds, 60°/second, 180°/second, and 300°/second using the Biodex 3 Isokinetic Dynamometer. Cortex 8.1 Motion Analysis Software was used to record three maximal kicks with the dominant leg. Bivariate Pearson correlation coefficients were calculated between both data sets using SPSS version 28. Results: Ball velocity was significantly and positively correlated with Right Leg Flexion Acceleration time at 60°/second(r= 0.860),[GJR4] Left Leg Extension Acceleration at 180°/second (r= 0.950), and Left Leg Extension Acceleration at 300°/second (r= 0.915). Two significant negative relationships were discovered between ball velocity and left leg extension acceleration at 300°/second (r= -0.950), and left angle of peak extension torque at 300°/second (r= - 0.915). Conclusion: The ability to quickly accelerate the non-kicking leg to extension combined with the ability to reach angle of peak extension torque is associated with the ability to quickly stabilize the plant leg. Flexion of the kicking leg at a lower angular velocity corresponds with a higher force production and when combined with a positive correlation to ball velocity, suggests increased loading of the kicking leg prior to ball contact. Lastly, the negative correlation between ball velocity and kicking-leg extension acceleration would suggest that faster acceleration leads to increased ball velocity. Because of this, isokinetic testing of the quadricep and hamstring strength is likely a good predictor of kicking velocity. Further testing is required to determine if present correlations are applicable to other populations of soccer athletes, which can affect training and return-to-play practices

Application of multiplexed ion mobility spectrometry towards the identification of host protein signatures of treatment effect in pulmonary tuberculosis.

RationaleThe monitoring of TB treatments in clinical practice and clinical trials relies on traditional sputum-based culture status indicators at specific time points. Accurate, predictive, blood-based protein markers would provide a simpler and more informative view of patient health and response to treatment.ObjectiveWe utilized sensitive, high throughput multiplexed ion mobility-mass spectrometry (IM-MS) to characterize the serum proteome of TB patients at the start of and at 8 weeks of rifamycin-based treatment. We sought to identify treatment specific signatures within patients as well as correlate the proteome signatures to various clinical markers of treatment efficacy.MethodsSerum samples were collected from 289 subjects enrolled in CDC TB Trials Consortium Study 29 at time of enrollment and at the end of the intensive phase (after 40 doses of TB treatment). Serum proteins were immunoaffinity-depleted of high abundant components, digested to peptides and analyzed for data acquisition utilizing a unique liquid chromatography IM-MS platform (LC-IM-MS). Linear mixed models were utilized to identify serum protein changes in the host response to antibiotic treatment as well as correlations with culture status end points.ResultsA total of 10,137 peptides corresponding to 872 proteins were identified, quantified, and used for statistical analysis across the longitudinal patient cohort. In response to TB treatment, 244 proteins were significantly altered. Pathway/network comparisons helped visualize the interconnected proteins, identifying up regulated (lipid transport, coagulation cascade, endopeptidase activity) and down regulated (acute phase) processes and pathways in addition to other cross regulated networks (inflammation, cell adhesion, extracellular matrix). Detection of possible lung injury serum proteins such as HPSE, significantly downregulated upon treatment. Analyses of microbiologic data over time identified a core set of serum proteins (TTHY, AFAM, CRP, RET4, SAA1, PGRP2) which change in response to treatment and also strongly correlate with culture status. A similar set of proteins at baseline were found to be predictive of week 6 and 8 culture status.ConclusionA comprehensive host serum protein dataset reflective of TB treatment effect is defined. A repeating set of serum proteins (TTHY, AFAM, CRP, RET4, SAA1, PGRP2, among others) were found to change significantly in response to treatment, to strongly correlate with culture status, and at baseline to be predictive of future culture conversion. If validated in cohorts with long term follow-up to capture failure and relapse of TB, these protein markers could be developed for monitoring of treatment in clinical trials and in patient care

Incorporating chemical signalling factors into cell-based models of growing epithelial tissues

In this paper we present a comprehensive computational framework within which the effects of chemical signalling factors on growing epithelial tissues can be studied. The method incorporates a vertex-based cell model, in conjunction with a solver for the governing chemical equations. The vertex model provides a natural mesh for the finite element method (FEM), with node movements determined by force laws. The arbitrary Lagrangian–Eulerian formulation is adopted to account for domain movement between iterations. The effects of cell proliferation and junctional rearrangements on the mesh are also examined. By implementing refinements of the mesh we show that the finite element (FE) approximation converges towards an accurate numerical solution. The potential utility of the system is demonstrated in the context of Decapentaplegic (Dpp), a morphogen which plays a crucial role in development of the Drosophila imaginal wing disc. Despite the presence of a Dpp gradient, growth is uniform across the wing disc. We make the growth rate of cells dependent on Dpp concentration and show that the number of proliferation events increases in regions of high concentration. This allows hypotheses regarding mechanisms of growth control to be rigorously tested. The method we describe may be adapted to a range of potential application areas, and to other cell-based models with designated node movements, to accurately probe the role of morphogens in epithelial tissues

A Search for Amino Acids and Nucleobases in the Martian Meteorite Roberts Massif 04262 Using Liquid Chromatography-Mass Spectrometry

The investigation into whether Mars contains signatures of past or present life is of great interest to science and society. Amino acids and nucleobases are compounds that are essential for all known life on Earth and are excellent target molecules in the search for potential Martian biomarkers or prebiotic chemistry. Martian meteorites represent the only samples from Mars that can be studied directly in the laboratory on Earth. Here, we analyzed the amino acid and nucleobase content of the shergottite Roberts Massif (RBT) 04262 using liquid chromatography-mass spectrometry. We did not detect any nucleobases above our detection limit in formic acid extracts; however, we did measure a suite of protein and nonprotein amino acids in hot-water extracts with high relative abundances of beta-alanine and gamma-amino-eta-butyric acid. The presence of only low (to absent) levels of several proteinogenic amino acids and a lack of nucleobases suggest that this meteorite fragment is fairly uncontaminated with respect to these common biological compounds. The distribution of straight-chained amine-terminal eta-omega-amino acids in RBT 04262 resembled those previously measured in thermally altered carbonaceous meteorites. A carbon isotope ratio of -24(0/00) +/- 6(0/00) for beta-alanine in RBT 04262 is in the range of reduced organic carbon previously measured in Martian meteorites (Steele et al. 2012). The presence of eta-omega-amino acids may be due to a high temperature Fischer-Tropschtype synthesis during igneous processing on Mars or impact ejection of the meteorites from Mars, but more experimental data are needed to support these hypotheses

Black Hole Mass Measurements of Radio Galaxies NGC 315 and NGC 4261 Using ALMA CO Observations

We present Atacama Large Millimeter/submillimeter Array (ALMA) Cycle 5 and Cycle 6 observations of CO(2$-$1) and CO(3$-$2) emission at 0.2''$-$0.3'' resolution in two radio-bright, brightest group/cluster early-type galaxies, NGC 315 and NGC 4261. The data resolve CO emission that extends within their black hole (BH) spheres of influence ($r_\mathrm{g}$), tracing regular Keplerian rotation down to just tens of parsecs from the BHs. The projected molecular gas speeds in the highly inclined ($i>60^\circ$) disks rises at least 500 km s$^{-1}$ near their galaxy centers. We fit dynamical models of thin-disk rotation directly to the ALMA data cubes, and account for the extended stellar mass distributions by constructing galaxy surface brightness profiles corrected for a range of plausible dust extinction values. The best-fit models yield $(M_\mathrm{BH}/10^9\,M_\odot)=2.08\pm0.01(\mathrm{stat})^{+0.32}_{-0.14}(\mathrm{sys})$ for NGC 315 and $(M_\mathrm{BH}/10^9\,M_\odot)=1.67\pm0.10(\mathrm{stat})^{+0.39}_{-0.24}(\mathrm{sys})$ for NGC 4261, the latter of which is larger than previous estimates by a factor of $\sim$3. The BH masses are broadly consistent with the relations between BH masses and host galaxy properties. These are among the first ALMA observations to map dynamically cold gas kinematics well within the BH-dominated regions of radio galaxies, resolving the respective $r_\mathrm{g}$ by factors of $\sim$5$-$10. The observations demonstrate ALMA's ability to precisely measure BH masses in active galaxies, which will enable more confident probes of accretion physics for the most massive galaxies.Comment: 24 pages, 11 figures, 4 tables. Accepted for publication in Ap

The metabolome regulates the epigenetic landscape during naive-to-primed human embryonic stem cell transition.

For nearly a century developmental biologists have recognized that cells from embryos can differ in their potential to differentiate into distinct cell types. Recently, it has been recognized that embryonic stem cells derived from both mice and humans exhibit two stable yet epigenetically distinct states of pluripotency: naive and primed. We now show that nicotinamide N-methyltransferase (NNMT) and the metabolic state regulate pluripotency in human embryonic stem cells (hESCs).  Specifically, in naive hESCs, NNMT and its enzymatic product 1-methylnicotinamide are highly upregulated, and NNMT is required for low S-adenosyl methionine (SAM) levels and the H3K27me3 repressive state. NNMT consumes SAM in naive cells, making it unavailable for histone methylation that represses Wnt and activates the HIF pathway in primed hESCs. These data support the hypothesis that the metabolome regulates the epigenetic landscape of the earliest steps in human development

TraDIS-Xpress: a high-resolution whole-genome assay identifies novel mechanisms of triclosan action and resistance

Understanding the genetic basis for a phenotype is a central goal in biological research. Much has been learnt about bacterial genomes by creating large mutant libraries and looking for conditionally important genes. However, current genome-wide methods are largely unable to assay essential genes which are not amenable to disruption. To overcome this limitation, we developed a new version of “TraDIS” (transposon directed insertion-site sequencing) that we term “TraDIS-Xpress” that combines an inducible promoter into the transposon cassette. This allows controlled overexpression and repression of all genes owing to saturation of inserts adjacent to all open reading frames as well as conventional inactivation. We applied TraDIS-Xpress to identify responses to the biocide triclosan across a range of concentrations. Triclosan is endemic in modern life, but there is uncertainty about its mode of action with a concentration-dependent switch from bacteriostatic to bactericidal action unexplained. Our results show a concentration-dependent response to triclosan with different genes important in survival between static and cidal exposures. These genes include those previously reported to have a role in triclosan resistance as well as a new set of genes, including essential genes. Novel genes identified as being sensitive to triclosan exposure include those involved in barrier function, small molecule uptake, and integrity of transcription and translation. We anticipate the approach we show here, by allowing comparisons across multiple experimental conditions of TraDIS data, and including essential genes, will be a starting point for future work examining how different drug conditions impact bacterial survival mechanisms

Self-Reported Cognitive Function and Mental Health Diagnoses among Former Professional American-Style Football Players

Clinical practice strongly relies on patients' self-report. Former professional American-style football players are hesitant to seek help for mental health problems, but may be more willing to report cognitive symptoms. We sought to assess the association between cognitive symptoms and diagnosed mental health problems and quality of life among a cohort of former professional players. In a cross-sectional design, we assessed self-reported cognitive function using items from the Quality of Life in Neurological Disorders (Neuro-QOL) Item Bank. We then compared mental health diagnoses and quality of life, assessed by items from the Patient-Reported Outcome Measurement Information System (PROMIS ®), between former professional players reporting daily problems in cognitive function and former players not reporting daily cognitive problems. Of the 3758 former professional players included in the analysis, 40.0% reported daily problems due to cognitive dysfunction. Former players who reported daily cognitive problems were more likely to also report depression (18.0% vs. 3.3%, odds ratio [OR] = 6.42, 95% confidence interval [CI] [4.90-8.40]) and anxiety (19.1% vs. 4.3%, OR = 5.29, 95% CI [4.14-6.75]) than those without daily cognitive problems. Further, former players reporting daily cognitive problems were more likely to report memory loss and attention deficit(/hyperactivity) disorder and poorer general mental health, lower quality of life, less satisfaction with social activities and relationships, and more emotional problems. These findings highlight the potential of an assessment of cognitive symptoms for identifying former players with mental health, social, and emotional problems