A Procedure for Performing Nonlinear Pushover Analysis for Tsunami Loading to ASCE 7

The new ASCE 7-16 Chapter 6 offers a comprehensive and practical methodology for the design of structures for tsunami loads and effects. While it provides prescriptive tsunami loading and design requirements, Chapter 6 also allows for the use of performance-based nonlinear analysis tools. However, the specifics of load application protocol and system and component evaluation for such a nonlinear approach are not provided. This paper presents a procedure for performing nonlinear static pushover analysis for tsunami loading within the framework of the ASCE 7-16 standard. Through this approach, the user can both estimate the effective systemic lateral load-resisting capacity of a building and the local component demand. This enables the identification of deficiencies in structural elements with respect to the ASCE 7-16 standard acceptance criteria. To demonstrate the procedure, a prototypical reinforced concrete multistory building exposed to high tsunami hazard on the US Northwest Pacific Coast is assessed. This is a building with sufficient height to provide last-resort refuge for people having insufficient time to evacuate outside the inundation zone. The results of the nonlinear static pushover analyses show that the structural system has sufficient lateral strength to resist ASCE 7-16 prescribed tsunami loads, but fails the checks for component-based loading, with the exterior ground-story columns observed to fail in flexure and shear. The example demonstrates that use of the tsunami nonlinear static analysis procedure allows the identification of structural deficiencies such that a targeted strengthening of the building can be conducted (i.e., flexural and shear strengthening of the seaward and inland columns for the case study building presented), leading to significantly reduced costs

A nonlinear static procedure for the tsunami design of a reinforced concrete building to the ASCE7 Standard

New ASCE 7-16 Chapter 6 offer a comprehensive and practical methodology for the design of structures for tsunami loads and effects. While they provide prescriptive tsunami loading and design requirements, they also allow for the use of performance-based analysis tools. However, no guidance is provided as to how the performance-based analysis should be performed. This paper presents an improved nonlinear static pushover procedure for the assessment of the nonlinear capacity of structures to tsunami, within the framework of the ASCE 7-16 provisions. For this purpose, a prototypical reinforced concrete multi-storey building exposed to high tsunami hazard in the US Northwest Pacific coast is assessed. This is a building with sufficient height to provide last-resort refuge for people having insufficient time to evacuate outside the inundation zone. Two different tsunami load discretisation methods are applied to investigate the structural capacity under tsunami systemic and component loading, respectively. The results of the nonlinear static pushover analyses show that the structural system has sufficient lateral strength to resist ASCE 7-16 prescribed tsunami loads. However, when component-based loading is considered, the seaward ground storey columns are observed to fail in shear, precipitating structural failure. This is in agreement with the ASCE 7-16 simplified systemic acceptance criteria, i.e. that the structure is unsafe for use as a refuge, and that it would require significant strengthening. However, the use of the VDPO provides information of what needs to be strengthened in order to improve the tsunami performance of the structure

The Cosmological Switchback Effect

The volume behind the black hole horizon was suggested as a holographic dual for the quantum computational complexity of the boundary state in AdS/CFT. This identification is strongly motivated by the switchback effect: a characteristic delay of complexity growth in reaction to an inserted perturbation, modelled as a shockwave in the bulk. Recent proposals of de Sitter (dS) holography suggest that a dual theory could be living on a stretched horizon near the cosmological horizon. We study how the spacetime volume behind the cosmological horizon in Schwarzschild-dS space reacts to the insertion of shockwaves in an attempt to characterize the properties of this dual theory. We demonstrate that a switchback effect can be observed in dS space. That is, the growth of complexity is delayed in reaction to a perturbation. This delay is longer for earlier shocks and depends on a scrambling time which is logarithmic in the strength of the shockwave and proportional to the inverse temperature of the cosmological dS horizon. This behavior is very similar to what happens for AdS black holes, albeit the geometric origin of the effect is different.Comment: 78 pages, 30 figure

Fragility functions for a reinforced concrete structure subjected to earthquake and tsunami in sequence

Many coastal regions lying on subduction zones are likely to experience the catastrophic effects of cascading earthquake and tsunami observed in recent events, e.g., 2011 Tohoku Earthquake and Tsunami. The influence of earthquake on the response of the structure to tsunami is difficult to quantify through damage observations from past events, since they only provide information on the combined effects of both perils. Hence, the use of analytical methodologies is fundamental. This paper investigates the response of a reinforced concrete frame subjected to realistic ground motion and tsunami inundation time histories that have been simulated considering a seismic source representative of the M9 2011 Tohoku earthquake event. The structure is analysed via nonlinear time-history analyses under (a) tsunami inundation only and (b) earthquake ground motion and tsunami inundation in sequence. Comparison of these analyses shows that there is a small impact of the preceding earthquake ground shaking on the tsunami fragility. The fragility curves constructed for the cascading hazards show less than 15% reduction in the median estimate of tsunami capacity compared to the fragility functions for tsunami only. This outcome reflects the fundamentally different response of the structure to the two perils: while the ground motion response of the structure is governed by its strength, ductility and stiffness, the tsunami performance of the structure is dominated by its strength. It is found that the ground shaking influences the tsunami displacement response of the considered structure due to the stiffness degradation induced in the ground motion cyclic response, but this effect decreases with increasing tsunami force

Glutamatergic neurons induce expression of functional glutamatergic synapses in primary myotubes.

The functioning of the nervous system depends upon the specificity of its synaptic contacts. The mechanisms triggering the expression of the appropriate receptors on postsynaptic membrane and the role of the presynaptic partner in the differentiation of postsynaptic structures are little known.To address these questions we cocultured murine primary muscle cells with several glutamatergic neurons, either cortical, cerebellar or hippocampal. Immunofluorescence and electrophysiology analyses revealed that functional excitatory synaptic contacts were formed between glutamatergic neurons and muscle cells. Moreover, immunoprecipitation and immunofluorescence experiments showed that typical anchoring proteins of central excitatory synapses coimmunoprecipitate and colocalize with rapsyn, the acetylcholine receptor anchoring protein at the neuromuscular junction.These results support an important role of the presynaptic partner in the induction and differentiation of the postsynaptic structures

Glutamatergic Reinnervation and Assembly of Glutamatergic Synapses in Adult Rat Skeletal Muscle Occurs at Cholinergic Endplates

After denervation of adult rat abdominal muscles, the postsynaptic apparatus of neuromuscular junctions (NMJs) retains its original architecture and clustering of acetylcholine receptors (AChRs). When descending fibers of the spinal cord are surgically diverted to this muscle by a nerve grafting procedure, supraspinal glutamatergic neurons can innervate muscle fibers and restore motor function; the newly formed NMJs switch from a cholinergic to a glutamatergic-type synapse. We show here that regenerating nerve endings contact the fibers in an area occupied by cholinergic endplates. These NMJs are morphologically indistinguishable from those in controls, but they differ in the subunit composition of AChRs. Moreover, by immunofluorescence and immunoelectron microscopy, new NMJs express glutamatergic synapse markers. The \u3b1-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) receptor subunit GluR1 partially colocalizes with AChRs, and vesicular glutamate transporter 2 is localized in the presynaptic compartment. Immunoprecipitation analysis of membranes from reinnervated muscle showed that AMPA receptor subunits GluR1 and GluR2 coimmunoprecipitate with rapsyn, the AChR-anchoring protein at the NMJ. Taken together, these results indicate that cholinergic endplates can be targeted by new glutamatergic projections and that the clustering of AMPA receptors occurs there

Late-onset Parkinsonism in NFκB/c-Rel-deficient mice.

Activation of the nuclear factor \u3baB/c-Rel can increase neuronal resilience to pathological noxae by regulating the expression of pro-survival manganese superoxide dismutase (MnSOD, now known as SOD2) and Bcl-xL genes. We show here that c-Rel-deficient (c-rel(-/-)) mice developed a Parkinson's disease-like neuropathology with ageing. At 18 months of age, c-rel(-/-) mice exhibited a significant loss of dopaminergic neurons in the substantia nigra pars compacta, as assessed by tyrosine hydroxylase-immunoreactivity and Nissl staining. Nigral degeneration was accompanied by a significant loss of dopaminergic terminals and a significant reduction of dopamine and homovanillic acid levels in the striatum. Mice deficient of the c-Rel factor exhibited a marked immunoreactivity for fibrillary \u3b1-synuclein in the substantia nigra pars compacta as well as increased expression of divalent metal transporter 1 (DMT1) and iron staining in both the substantia nigra pars compacta and striatum. Aged c-rel(-/-) mouse brain were characterized by increased microglial reactivity in the basal ganglia, but no astrocytic reaction. In addition, c-rel(-/-) mice showed age-dependent deficits in locomotor and total activity and various gait-related deficits during a catwalk analysis that were reminiscent of bradykinesia and muscle rigidity. Both locomotor and gait-related deficits recovered in c-rel(-/-) mice treated with l-3,4-dihydroxyphenylalanine. These data suggest that c-Rel may act as a regulator of the substantia nigra pars compacta resilience to ageing and that aged c-rel(-/-) mice may be a suitable model of Parkinson's disease

Late-onset Parkinsonism in NFκB/c-Rel-deficient mice

Activation of the nuclear factor κB/c-Rel can increase neuronal resilience to pathological noxae by regulating the expression of pro-survival manganese superoxide dismutase (MnSOD, now known as SOD2) and Bcl-xL genes. We show here that c-Rel-deficient (c-rel(-/-)) mice developed a Parkinson's disease-like neuropathology with ageing. At 18 months of age, c-rel(-/-) mice exhibited a significant loss of dopaminergic neurons in the substantia nigra pars compacta, as assessed by tyrosine hydroxylase-immunoreactivity and Nissl staining. Nigral degeneration was accompanied by a significant loss of dopaminergic terminals and a significant reduction of dopamine and homovanillic acid levels in the striatum. Mice deficient of the c-Rel factor exhibited a marked immunoreactivity for fibrillary α-synuclein in the substantia nigra pars compacta as well as increased expression of divalent metal transporter 1 (DMT1) and iron staining in both the substantia nigra pars compacta and striatum. Aged c-rel(-/-) mouse brain were characterized by increased microglial reactivity in the basal ganglia, but no astrocytic reaction. In addition, c-rel(-/-) mice showed age-dependent deficits in locomotor and total activity and various gait-related deficits during a catwalk analysis that were reminiscent of bradykinesia and muscle rigidity. Both locomotor and gait-related deficits recovered in c-rel(-/-) mice treated with l-3,4-dihydroxyphenylalanine. These data suggest that c-Rel may act as a regulator of the substantia nigra pars compacta resilience to ageing and that aged c-rel(-/-) mice may be a suitable model of Parkinson's disease

Characterization of Stem-Like Cells in Mucoepidermoid Tracheal Paediatric Tumor

Stem cells contribute to regeneration of tissues and organs. Cells with stem cell-like properties have been identified in tumors from a variety of origins, but to our knowledge there are yet no reports on tumor-related stem cells in the human upper respiratory tract. In the present study, we show that a tracheal mucoepidermoid tumor biopsy obtained from a 6 year-old patient contained a subpopulation of cells with morphology, clonogenicity and surface markers that overlapped with bone marrow mesenchymal stromal cells (BM-MSCs). These cells, designated as MEi (mesenchymal stem cell-like mucoepidermoid tumor) cells, could be differentiated towards mesenchymal lineages both with and without induction, and formed spheroids in vitro. The MEi cells shared several multipotent characteristics with BM-MSCs. However, they displayed differences to BM-MSCs in growth kinectics and gene expression profiles relating to cancer pathways and tube development. Despite this, the MEi cells did not possess in vivo tumor-initiating capacity, as proven by the absence of growth in situ after localized injection in immunocompromised mice. Our results provide an initial characterization of benign tracheal cancer-derived niche cells. We believe that this report could be of importance to further understand tracheal cancer initiation and progression as well as therapeutic development

Rapid production of human liver scaffolds for functional tissue engineering by high shear stress oscillation-decellularization

The development of human liver scaffolds retaining their 3-dimensional structure and extra-cellular matrix (ECM) composition is essential for the advancement of liver tissue engineering. We report the design and validation of a new methodology for the rapid and accurate production of human acellular liver tissue cubes (ALTCs) using normal liver tissue unsuitable for transplantation. The application of high shear stress is a key methodological determinant accelerating the process of tissue decellularization while maintaining ECM protein composition, 3D-architecture and physico-chemical properties of the native tissue. ALTCs were engineered with human parenchymal and non-parenchymal liver cell lines (HepG2 and LX2 cells, respectively), human umbilical vein endothelial cells (HUVEC), as well as primary human hepatocytes and hepatic stellate cells. Both parenchymal and non-parenchymal liver cells grown in ALTCs exhibited markedly different gene expression when compared to standard 2D cell cultures. Remarkably, HUVEC cells naturally migrated in the ECM scaffold and spontaneously repopulated the lining of decellularized vessels. The metabolic function and protein synthesis of engineered liver scaffolds with human primary hepatocytes reseeded under dynamic conditions were maintained. These results provide a solid basis for the establishment of effective protocols aimed at recreating human liver tissue in vitro