Virtual teaching kitchen classes and cardiovascular disease prevention counselling among medical trainees

Background: Hands-on culinary medicine education for medical trainees has emerged as a promising tool for cardiovascular health promotion. Purpose: To determine whether virtual culinary medicine programming associates with Mediterranean diet (MedDiet) adherence and lifestyle medicine competencies among medical trainees across the USA. Method: A total of 1433 medical trainees across 19 sites over a 12-month period were included. The Cooking for Health Optimisation with Patients-Medical Trainees survey composed of 61 questions regarding demographics, nutritional attitudes, dietary habits including MedDiet score and lifestyle medicine counselling competencies. Multivariable logistic regression assessed the association of virtual culinary medicine education with MedDiet intake and nutritional attitudes. Results: There were 519 medical trainees who participated in virtual culinary medicine education and 914 medical trainees who participated in their standard nutrition curricula. More than one-half of participants were women (n=759) and the mean age was 27 years old. Compared with students enrolled in traditional nutrition curricula, participants in virtual culinary medicine education were 37% more likely to adhere to MedDiet guidelines for fruit intake (OR 1.37, 95% CI 1.03 to 1.83, p=0.03). Virtual culinary medicine education was associated with higher proficiency in lifestyle medicine counselling categories, notably recommendations involving fibre (OR 4.03; 95% CI 3.05 to 5.34), type 2 diabetes prevention (OR 4.69; 95% CI 3.51 to 6.27) and omega fatty acids (OR 5.21; 95% CI 3.87 to 7.02). Virtual culinary medicine education had a similar, although higher magnitude association with MedDiet counselling competency (OR 5.73, 95% CI 4.26 to 7.70) when compared with historical data previously reported using hands-on, in-person culinary medicine courseware (OR 4.97, 95% CI 3.89 to 6.36). Conclusions: Compared with traditional nutritional educational curricula, virtual culinary medicine education is associated with higher MedDiet adherence and lifestyle medicine counselling competencies among medical trainees. Both virtual and hands-on culinary medicine education may be useful for cardiovascular health promotion

Epstein-Barr Virus LMP2A Reduces Hyperactivation Induced by LMP1 to Restore Normal B Cell Phenotype in Transgenic Mice

Epstein-Barr virus (EBV) latently infects most of the human population and is strongly associated with lymphoproliferative disorders. EBV encodes several latency proteins affecting B cell proliferation and survival, including latent membrane protein 2A (LMP2A) and the EBV oncoprotein LMP1. LMP1 and LMP2A signaling mimics CD40 and BCR signaling, respectively, and has been proposed to alter B cell functions including the ability of latently-infected B cells to access and transit the germinal center. In addition, several studies suggested a role for LMP2A modulation of LMP1 signaling in cell lines by alteration of TRAFs, signaling molecules used by LMP1. In this study, we investigated whether LMP1 and LMP2A co-expression in a transgenic mouse model alters B cell maturation and the response to antigen, and whether LMP2A modulates LMP1 function. Naïve LMP1/2A mice had similar lymphocyte populations and antibody production by flow cytometry and ELISA compared to controls. In the response to antigen, LMP2A expression in LMP1/2A animals rescued the impairment in germinal center generation promoted by LMP1. LMP1/2A animals produced high-affinity, class-switched antibody and plasma cells at levels similar to controls. In vitro, LMP1 upregulated activation markers and promoted B cell hyperproliferation, and co-expression of LMP2A restored a wild-type phenotype. By RT-PCR and immunoblot, LMP1 B cells demonstrated TRAF2 levels four-fold higher than non-transgenic controls, and co-expression of LMP2A restored TRAF2 levels to wild-type levels. No difference in TRAF3 levels was detected. While modulation of other TRAF family members remains to be assessed, normalization of the LMP1-induced B cell phenotype through LMP2A modulation of TRAF2 may be a pathway by which LMP2A controls B cell function. These findings identify an advance in the understanding of how Epstein-Barr virus can access the germinal center in vivo, a site critical for both the genesis of immunological memory and of virus-associated tumors

Mitochondrial Alterations in PINK1 Deficient Cells Are Influenced by Calcineurin-Dependent Dephosphorylation of Dynamin-Related Protein 1

PTEN-induced novel kinase 1 (PINK1) mutations are associated with autosomal recessive parkinsonism. Previous studies have shown that PINK1 influences both mitochondrial function and morphology although it is not clearly established which of these are primary events and which are secondary. Here, we describe a novel mechanism linking mitochondrial dysfunction and alterations in mitochondrial morphology related to PINK1. Cell lines were generated by stably transducing human dopaminergic M17 cells with lentiviral constructs that increased or knocked down PINK1. As in previous studies, PINK1 deficient cells have lower mitochondrial membrane potential and are more sensitive to the toxic effects of mitochondrial complex I inhibitors. We also show that wild-type PINK1, but not recessive mutant or kinase dead versions, protects against rotenone-induced mitochondrial fragmentation whereas PINK1 deficient cells show lower mitochondrial connectivity. Expression of dynamin-related protein 1 (Drp1) exaggerates PINK1 deficiency phenotypes and Drp1 RNAi rescues them. We also show that Drp1 is dephosphorylated in PINK1 deficient cells due to activation of the calcium-dependent phosphatase calcineurin. Accordingly, the calcineurin inhibitor FK506 blocks both Drp1 dephosphorylation and loss of mitochondrial integrity in PINK1 deficient cells but does not fully rescue mitochondrial membrane potential. We propose that alterations in mitochondrial connectivity in this system are secondary to functional effects on mitochondrial membrane potential

Exploring Wikidata "Archives At" at U of T

Presented to the LD4 Wikidata Affinity Group community on April 20, 2021. Presentation discusses U of T's exploration into surfacing online finding aids found in its Discover Archives repository through Wikidata's "archives at" property

Knowledge Lost, Knowledge Gained : The Implications of Migrating to Online Archival Descriptive Systems

Migrating archival description from paper-based finding aids to structured online data reconfigures the dynamics of archival representation and interactions. This paper considers the knowledge implications of transferring traditional finding aids to Discover Archives, a university-wide implementation of Access to Memory (AtoM) at the University of Toronto. The migration and translation of varied descriptive practices to conform to a single system that is accessible to anyone, anywhere, effectively shifts both where and how users interface with archives and their material. This paper reflects on how different sets of knowledge are reorganized in these shifts. Discover Archives empowers researchers to do independent searches using the full breadth of their domain expertise, seemingly unbound from archival gatekeeping. At the same time, these searches are performed in the absence of archivists' unstructured mediation, where searches benefit from human interaction and the kinds of knowledges that reference staff draw on to handle complex reference questions, especially those from novice archival users. We explore the extent to which that lost knowledge can be drawn back into archival interactions via rich metadata that documents contexts and relationships embedded within Discover Archives and beyond. Internal user experience design (UXD) research on Discover Archives highlights a gap between current online description and habitual user expectations in web search and discovery. To help bridge this gap, we contributed to broader discovery nodes such as linked open "context hubs" like Wikipedia and Wikidata, which can supplement hierarchical description with linked metadata and visualization capabilities. These can reintroduce rhizomatic and serendipitous connections, enabled by archivist, researcher, and larger sets of community knowledges, to the benefit of both the user and the archivist

Formula SAE Electric Car

Formula SAE Hybrid Competition is an annual design and engineering collegiate challenge. The goal of this year’s FSAE team was to design, build, and compete with a formula-style electric, open-wheel race car. This project brought together an interdisciplinary team of 21 students from ME, ECE, RBE, and CS. Despite the constraints, which included: competition rules, finances and time required to complete the race car, our team members conceived and manufactured several components: drivetrain, suspension, custom battery, tractive system components, charger, and braking to satisfy design requirements and structural FEA. Additionally, various circuits were designed and built to meet the rules, including shutdown, pre-charge and discharge, brake system plausibility, tractive system active light, and additional printed circuit boards for optimization and testing. We worked on the integration of the mechanical systems with the electrical systems to obtain a fully operational race car