501 research outputs found
Lit de la Seine
Le Groupement de recherches archĂ©ologiques subaquatiques a poursuivi ses prospections en vue dâidentifier de nouveaux sites sur le cours de la Haute-Seine. Ă Ătiolles (91), une prospection menĂ©e en amont des lies-aux-Paveurs, a rĂ©vĂ©lĂ© une digue empierrĂ©e submergĂ©e qui barre partiellement le petit bras de la Seine (xixe s.). Ă la pointe de lâĂźle, une ligne de quelques pieux dâune dizaine de centimĂštres de diamĂštre, a Ă©tĂ© localisĂ©e. Ils sont enfoncĂ©s dans une couche de falaize et le bois qui le..
Lit de la Seine entre HĂ©ricy et Corbeil-Essonnes
Le Groupement de recherches archĂ©ologiques subaquatiques a poursuivi ses prospections en vue dâidentifier de nouveaux sites sur le cours de la Haute-Seine.Ă HĂ©ricy (77), une prospection a Ă©tĂ© rĂ©alisĂ©e Ă lâemplacement de lâancien pont disparu afin de repĂ©rer dâĂ©ventuels vestiges et de tenter une datation de lâouvrage. Il semble que les piles ont Ă©tĂ© entiĂšrement arasĂ©es pour les besoins de la navigation car aucun relief ne traduit leur prĂ©sence au fond. Les seuls vestiges observĂ©s ont Ă©tĂ© loca..
Histoire de lâhabitat
Augustin Berque, directeur dâĂ©tudes avec Philippe Bonnin et Cynthia Ghorra-Gobin, directeur de recherche au CNRS Cf. le compte rendu Ă la rubrique « GĂ©ographie »
Anthropologie de lâhabiter
Augustin Berque, directeur dâĂ©tudesPhilippe Bonnin, directeur dâĂ©tudes au CNRSAlessia de Biase, maĂźtre-assistant Ă lâENSAPB PoĂ©tique de lâhabiter Les premiĂšres sĂ©ances du sĂ©minaire (ensuite interrompu pour raisons de santĂ©) ont Ă©tĂ© consacrĂ©es au thĂšme de la cosmicitĂ© chinoise traditionnelle. Le sĂ©minaire collectif sâest cependant poursuivi jusquâĂ la fin de lâannĂ©e en lâabsence du directeur dâĂ©tudes, avec notamment deux confĂ©rences de Tsuchiya sur le thĂšme ci-dessus mentionnĂ© Publications La ..
MĂ©thode d'optimisation temporelle des algorithmes de vision
- Compte tenu de la puissance de calcul disponible sur les robots mobiles, la recherche d'une optimisation temporelle devient un élément fondamental. Dans ce papier, une méthode est proposée, illustrée par deux applications réalisées. Enfin, nous présentons des résultats numériques, issus de « benchmarks » quantifiant les améliorations réalisées
Whole genome mapping of 5' RNA ends in bacteria by tagged sequencing : A comprehensive view in Enterococcus faecalis
Enterococcus faecalis is the third cause of nosocomial infections. To obtain
the first comprehensive view of transcriptional organizations in this
bacterium, we used a modified RNA-seq approach enabling to discriminate primary
from processed 5'RNA ends. We also validated our approach by confirming known
features in Escherichia coli.
We mapped 559 transcription start sites and 352 processing sites in E.
faecalis. A blind motif search retrieved canonical features of SigA- and
SigN-dependent promoters preceding TSSs mapped. We discovered 95 novel putative
regulatory RNAs, small- and antisense RNAs, and 72 transcriptional antisense
organisations.
Presented data constitute a significant insight into bacterial RNA landscapes
and a step towards the inference of regulatory processes at transcriptional and
post-transcriptional levels in a comprehensive manner
Transcriptional Landscape of a blaKPC-2 Plasmid and Response to Imipenem Exposure in Escherichia coli TOP10
The diffusion of KPC-2 carbapenemase is closely related to the spread of Klebsiella pneumoniae of the clonal-group 258 and linked to IncFIIK plasmids. Little is known about the biology of multi-drug resistant plasmids and the reasons of their successful dissemination. Using E. coli TOP10 strain harboring a multi-replicon IncFIIK-IncFIB blaKPCâ2-gene carrying plasmid pBIC1a from K. pneumoniae ST-258 clinical isolate BIC-1, we aimed to identify basal gene expression and the effects of imipenem exposure using whole transcriptome approach by RNA sequencing (RNA-Seq). Independently of the antibiotic pressure, most of the plasmid-backbone genes were expressed at low levels. The most expressed pBIC1a genes were involved in antibiotic resistance (blaKPCâ2, blaTEM and aph(3âČ)-I), in plasmid replication and conjugation, or associated to mobile elements. After antibiotic exposure, 34% of E. coli (pBIC1a) genome was differentially expressed. Induction of oxidative stress response was evidenced, with numerous upregulated genes of the SoxRS/OxyR oxydative stress regulons, the Fur regulon (for iron uptake machinery), and IscR regulon (for iron sulfur cluster synthesis). Nine genes carried by pBIC1a were up-regulated, including the murein DD-endopeptidase mepM and the copper resistance operon. Despite the presence of a carbapenemase, we observed a major impact on E. coli (pBIC1a) whole transcriptome after imipenem exposure, but no effect on the level of transcription of antimicrobial resistance genes. We describe adaptive responses of E. coli to imipenem-induced stress, and identified plasmid-encoded genes that could be involved in resistance to stressful environments
Anthropologie de lâhabiter
Augustin Berque, directeur dâĂ©tudesPhilippe Bonnin, directeur de recherche au CNRSAlessia de Biase, maĂźtre-assistant Ă lâENSAPB La poĂ©tique de lâhabiter Cf. le compte rendu du sĂ©minaire « Questions de mĂ©sologie », Ă la rubrique GĂ©ographie Augustin Berque, directeur dâĂ©tudesPhilippe Bonnin, directeur de recherche au CNRSMarc Bourdier, professeur Ă lâENSAPLVNicolas FiĂ©vĂ©, directeur dâĂ©tudes Ă lâEPHEMurielle Hladik, maĂźtre-assistante Ă lâENSA de Saint-ĂtienneCorinne Tiry, architecte DPLG, ingĂ©ni..
Deletion of the myeloid endothelin-B receptor confers long-term protection from angiotensin II-mediated renal, retinal & vascular injury
International audienceThe endothelin system may be an important player in hypertensive end-organ injury as endothelin-1 increases blood pressure and is pro-inflammatory. The immune system is emerging as an important regulator of blood pressure and we have shown that the early hypertensive response to angiotensin-II infusion was amplified in mice deficient of myeloid endothelin-B (ETB) receptors (LysM-CreEdnrblox/lox). Hypothesizing that these mice would display enhanced organ injury, we gave angiotensin-II to LysM-CreEdnrblox/lox and littermate controls (Ednrblox/lox) for six weeks. Unexpectedly, LysM-CreEdnrblox/lox mice were significantly protected from organ injury, with less proteinuria, glomerulosclerosis and inflammation of the kidney compared to controls. In the eye, LysM-CreEdnrblox/lox mice had fewer retinal hemorrhages, less microglial activation and less vessel rarefaction. Cardiac remodeling and dysfunction were similar in both groups at week six but LysM-CreEdnrblox/lox mice had better endothelial function. Although blood pressure was initially higher in LysM-CreEdnrblox/lox mice, this was not sustained. A natriuretic switch at about two weeks, due to enhanced ETB signaling in the kidney, induced a hypertensive reversal. By week six, blood pressure was lower in LysM-CreEdnrblox/lox mice than in controls. At six weeks, macrophages from LysM-CreEdnrblox/lox mice were more anti-inflammatory and had greater phagocytic ability compared to the macrophages of Ednrblox/lox mice. Thus, myeloid cell ETB receptor signaling drives this injury both through amplifying hypertension and by inflammatory polarization of macrophages
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