The 3Rs: reduction and refinement through a multivariate statistical analysis approach in a behavioural study to unveil anxiolytic effects of natural extracts of Tilia Tomentosa.

Proceedings of the second Centro 3R Annual Meeting - Young Scientists | June 20-21, 2019, Genoa (Italy

Use of an animal model to evaluate anxiolytic effects of dietary supplementation with tilia tomentosa moench bud extracts

Anxiety disorders are common and complex psychiatric syndromes affecting a broad spectrum of patients. On top of that, we know that aging produces an increase in anxiety vulnerability and sedative consumption. Moreover, stress disorders frequently show a clear gender susceptibility. Currently, the approved pharmacological strategies have severe side effects such as hallucinations, addiction, suicide, insomnia, and loss of motor coordination. Dietary integration with supplements represents an intriguing strategy for improving the efficacy and the safety of synthetic anxiolytics. Accordingly, a recent article demonstrated that glyceric bud extracts from Tilia tomentosa Moench (TTBEs) exert effects that are consistent with anxiolytic activity. However, the effects of these compounds in vivo are unknown. To examine this question, we conducted behavioral analysis in mice. A total of 21 days of oral supplements (vehicle and TTBEs) were assessed by Light Dark and Hole Board tests in male and female mice (young, 3 months; old, 24 months). Interestingly, the principal component analysis revealed gender and age-specific behavioral modulations. Moreover, the diet integration with the botanicals did not modify the body weight gain and the daily intake of water. Our results support the use of TTBEs as dietary supplements for anxiolytic purposes and unveil age and gender-dependent responses

Centrifugal Distortion Constants in 1.3 Propiolactone

The centrifugal distortion constants for 0-CH₂ -CH₂ -C = 0 were calculated using Dowling and Watson’s formalisms. From the fitting analysis and the deviation of predicted transitions it is concluded that the second formalism must be adopted. For the different parameters we have: A'=12406.012 ±0.005 MHz; B'=5244.456 ± 0.002 MHz; C'=3869.191 ± 0.002 MHz; dj= -2.08± 0 .05 kHz; djk= -23.6 3± 0.95 kHz; dk= - 33.43 ± 1.69 kHz; dWJ= (0.56± 0.01)X l0-6; dwK = (5.08 ± 0.22)X 10 -6.Publicado on line en 2014.Facultad de Ciencias Exacta

An EBIS for charge state breeding in the SPES project

The 'charge state breeder', BRIC (breeding ion charge) is in construction at the INFN section of Bari (Italy). It is based on EBIS scheme and it is designed to accept radioactive ion beam (RIB) with charge state +1 in a slow injection mode. This experiment can be considered as a first step towards the design and construction of a charge breeder for the SPES project. The new feature of BRIC, with respect to the classical EBIS, is given by the insertion, in the ion chamber, of a rf-quadrupole aiming at filtering the unwanted masses and then making a more efficient containment of the wanted ions. In this paper, the breeder design, the simulation results of the electron and ion beam propagation and the construction problems of the device will be reported

Genetic Algorithms as a Feature Selection Tool in Heart Failure Disease

A great wealth of information is hidden in clinical datasets, which could be analyzed to support decision-making processes or to better diagnose patients. Feature selection is one of the data pre-processing that selects a set of input features by removing unneeded or irrelevant features. Various algorithms have been used in healthcare to solve such problems involving complex medical data. This paper demonstrates how Genetic Algorithms offer a natural way to solve feature selection amongst data sets, where the fittest individual choice of variables is preserved over different generations. In this paper, a Genetic Algorithm is introduced as a feature selection method and shown to be effective in aiding understanding of such data

Looking at NB-IoT over LEO Satellite Systems: Design and Evaluation of a Service-Oriented Solution

The adoption of the NB-IoT technology in satellite communications intends to boost Internet of Things services beyond the boundaries imposed by the current terrestrial infrastructures. Apart from link-level studies in the scientific literature and preliminary 3GPP technical reports, the overall debate is still open. To provide a further step forward in this direction, the work presented herein pursues a novel service-oriented methodology to design an effective solution, meticulously stitched around application requirements and technological constraints. To this end, it conducts link-level and system-level investigations to tune physical transmissions, satellite constellation, and protocol architecture, while ensuring the expected system behavior. To offer a real smart agriculture service operating in Europe, the resulting solution exploits 24 Low Earth Orbit satellites, grouped into 8 different orbits, moving at an altitude of 500 km. The configured protocol stack supports the transmission of tens of bytes generated at the application layer, by also counteracting the issues introduced by the satellite link. Since each satellite has the whole protocol stack on-board, terminals can transmit data without the need for the feeder link. This ensures communication latencies ranging from 16 minutes to 75 minutes, depending on the served number of terminals and the physical transmission settings. Moreover, the usage of the Early Data Transmission scheme reduces communication latencies up to 40%. These results pave the way towards the deployment of an effective proof-of-concept, which drastically reduces the time-to-market imposed by the current state of the art

AWPP: A New Scheme for Wireless Access Control Proportional to Traffic Priority and Rate

Cutting-edge wireless networking approaches are required to efficiently differentiate traffic and handle it according to its special characteristics. The current Medium Access Control (MAC) scheme which is expected to be sufficiently supported by well-known networking vendors comes from the IEEE 802.11e workgroup. The standardized solution is the Hybrid Coordination Function (HCF), that includes the mandatory Enhanced Distributed Channel Access (EDCA) protocol and the optional Hybrid Control Channel Access (HCCA) protocol. These two protocols greatly differ in nature and they both have significant limitations. The objective of this work is the development of a high-performance MAC scheme for wireless networks, capable of providing predictable Quality of Service (QoS) via an efficient traffic differentiation algorithm in proportion to the traffic priority and generation rate. The proposed Adaptive Weighted and Prioritized Polling (AWPP) protocol is analyzed, and its superior deterministic operation is revealed

Phytochemical profile and antioxidant capacity of virgin olive oil obtained from the olive cultivar 'Roghiani' from different regions of northern Libya

The aim of this work was to determine the fatty acids (FA), tocopherols, phenols, and antioxidant capacity (AC) of five virgin olive oils (VOO) obtained from the olive cultivar 'Roghiani', from different regions of northern Libya during the crop year 2015. The parameters determined for the oils were investigated for the first time. Seven principal FAs were detected in the VOO samples. Oleic, palmitic, and linoleic acids were the dominant ones. The highest percentage of palmitic acid was found in Tripoli VOO (P lt = 0.05). Oleic acid was dominant in all the VOO samples. Gharyan VOO possessed the highest value of oleic acid, O/L ratio, monounsaturated fatty acids (MUFA), total phenols content (TPC), total tocopherol and tocotrienol contents (TTC), and AC(DPPH)% (P lt = 0.05). A strong positive relationship between TPC and (AC) as (1/EC50DPPH) was observed, (r) was +0.831. On the other hand, (1/EC50DPPH) showed a positive correlation with TTC, (r) was +0.768