Traffic Vertical Signposting: Materials Characterization and Structural Numerical Simulation

Aknowlegements: This work was supported by the project POCI-01-0247-FEDER-010797from the Agency for Competitiveness and Innovation (IAPMEI). In addition, the authors acknowledge the funding from the project UID/Multi/04044/2013 of the Portuguese Foundation for Science and Technology (FCT) and PAMI - ROTEIRO/0328/2013 (Nº 022158).The existing metallic solutions used for vertical traffic signs are associated with higher costs and environmental issues due to their manufacturing and degradation, when compared with polymeric solutions. Thus, the development of vertical signs considering the injection from polymeric materials in order to overcome problems related with sustainability, maintenance costs, and to achieve higher resistance to corrosion assumes nowadays an important role. The use of ecofriendly and innovative products considering the industrial waste combined with synthetic polymers performing the appropriate mechanical properties, can also be studied to find out new solutions that allow to solve the aforementioned problems. Additionally, these innovative vertical signs can contribute to avoid vandalism events related with theft and graffiti activities. This work presents the prior materials investigation and the structural design of vertical signs that are intended to be produced through polymer injection. Three main steps were considered: i) materials research, ii) materials characterisation through the analysis of polycarbonate resin isolated and in different sets of mixtures with different concentrations through tensile testing and static water contact angle measurements to find the optimal material composition; and iii) structural numerical simulation considering polycarbonate resin and using the current standard EN 12899-1 [1] to compute wind resistance, temporary and permanent deflections. Both experimental and numerical results led to an optimized proposal of the vertical signposting structural design.info:eu-repo/semantics/publishedVersio

Effect of Tween-80 on Stability and Secretion of Hydrophobic Tagged-cutinases

To significantly enhance the downstream processing, two cutinase variants were constructed by genetic fusion small hydrophobic peptides (WP)2 and (WP)4, respectively. However, the fusion of these peptides impairs cutinase secretion by the host cell Saccharomyces cerevisiae and increases cutinase inactivation in the culture broth due to cutinase aggregation, resulting in cutinase activities per biomass of 56 % of cutinase-(WP)2 and of 7 % of cutinase-(WP)4, in relation to cutinase without the hydrophobic tags. It was observed that the addition of non-ionic surfactant Tween-80 into the culture broth could minimise the cutinase inactivation. The addition of Tween-80 also results in the enhancement of cutinase secretion by the yeast cell, leading to 1.25 and 2.51 fold-higher extracellular cutinase-(WP)2 and cutinase-(WP)4, respectively, in relation to cultivations performed in the absence of surfactant. Therefore, the addition of Tween-80 on the culture broth partly minimises the effect of fusion of the hydrophobic tags on the inactivation of the enzymatic activity and on the reduction of the protein secretion. By this way, the use of Tween-80 on the S. cerevisiae cultivation may contribute to the efficiency enhancement of the downstream processing of tagged cutinases

An examination of intentions of recommending fitness centers by user members

The goal of this study was to examine what experiences members have with fitness centers that influence their intentions for recommendation. After item generation and content validity, as well as a pilot test, a survey was conducted among members of five different clubs (n=1750). The questionnaire included measures of service quality attributes, accessibility, well-being in life, well-being in a club, and intentions to recommend a fitness center. Results using a structural equation model provide evidence that only service quality attributes and well-being in a club have positive effect on intentions of users to recommend it. Well-being in life has a negative effect on the intentions for recommendation, while accessibility shows no predictive effect. These findings suggest implications for the management of a club, such as the need to create a pleasant environment and to provide a personalized service directed towards the members’ goals in order to improve well-being in a club and contribute to increase the intentions to recommend the fitness centers to others.O objetivo deste estudo foi examinar como as experiências de usuários de centros de atividade física interferem nas intenções em recomendar serviços. Depois de gerar itens e validar o conteúdo, aplicou-se um pré-teste, o questionário final foi aplicado a sócios de cinco clubes de fitness (n=1.750). O questionário incluiu atributos da qualidade do serviço, acessibilidade, bem-estar na vida, bem-estar no clube e intenção de recomendar. O modelo de equações estruturais mostrou que apenas os atributos da qualidade do serviço e bem-estar no clube têm um efeito positivo sobre as intenções de recomendar. O bem-estar na vida tem um efeito negativo sobre as intenções para recomendar, enquanto que a acessibilidade não mostra nenhum efeito preditivo. Estas conclusões sugerem a necessidade de criar um ambiente agradável nos clubes e de oferecer um serviço personalizado para os objetivos dos sócios, a fim de melhorar o bem-estar no clube e contribuir para a intenção de recomendar o ginásio. Palavras-chave: intenção de recomendar, atributos da qualSin financiación0.191 SJR (2014) Q3, posición 172/231 Health (social science), 1027/1811 Medicine (miscellaneous); Q4, 112/128 Sports scienceUE

The role of emotions on consumers’ satisfaction within the fitness context

Previous studies have suggested that consumption-related emotions are important to understand post-purchase reactions. This study examines the relationship between fitness consumers’ emotions and overall satisfaction. After an initial step of free-thought listing and content validity, followed by a pre-test, a survey was conducted among consumers of five different fitness centers (n=786). The questionnaire included measures to assess positive and negative emotions, as well as overall satisfaction with the fitness center. The results gathered through a structural equation model provide evidence that negative emotion experienced by consumers impacts negatively overall satisfaction, while positive emotion have a positive effect on overall satisfaction. These findings suggest managerial implications, such as the need to collect consumers’ perceptions of both tangible and intangible aspects of the services, listen costumers’ opinions in a regular basis, and provide regular training to staff members, in order to identify the triggers of positive emotions and contribute to increased levels of overall satisfaction. Guidelines for future research within the fitness context are also suggested.Estudos precedentes sugerem que as emoções relacionadas com o consumo são importantes para compreender as reações dos consumidores após a compra. Este estudo analisa a relação entre as emoções dos consumidores de fitness e satisfação global. Depois de uma etapa inicial de listagem de pensamento-livre e validade de conteúdo, seguido de um pré-teste, foi realizada uma pesquisa entre os consumidores de cinco centros de fitness diferentes (n = 786). O questionário incluiu medidas para avaliar as emoções positivas e negativas, bem como a satisfação global com o centro de fitness. Os resultados obtidos através de um modelo de equações estruturais forneceram evidências de que as emoções negativas vivenciadas pelos consumidores impactam negativamente a satisfação global, enquanto as emoções positivas têm um efeito positivo sobre a satisfação global. Estes resultados sugerem implicações para os gestores, tais como a necessidade de recolher informação sobre a perceção dos consumidores dos aspetos tangíveis e intangíveis dos serviços, ouvir regularmente as opiniões dos consumidores e facultar formação regular aos colaboradores. Isto permitirá identificar os aspetos que desencadeiam emoções positivas e contribuir para o aumento dos níveis de satisfação global. Orientações para futuras pesquisas no contexto de fitness também são sugeridas.Sin financiación0.185 SJR (2015) Q3, 1090/1779 Medicine (miscellaneous); Q4, 177/229 Health (social science), 112/128 Sports scienceUE

Performance indicators for clinical practice management in primary care in Portugal : consensus from a Delphi study

Early OnlineBackground: Performance indicators assessing the quality of medical care and linked to pay for performance may cause disagreement. Portuguese indicators included in recent health care reform are controversial. Objectives: To obtain consensus from opinion leaders in family medicine regarding the performance indicators for practice management used in the evaluation of Family Health Units in Portugal. Methods: Eighty-nine specialists in primary care were invited to answer the following question in an online Delphi study: 'Which performance indicators should be assessed regarding the organization and management of clinical practice in primary care in Portugal?' A Likert scale was used to evaluate validity, reliability, feasibility and sensitivity to change. Twenty-seven experts participated in the second round and achieved a high degree of consensus. Eight categories were created for analysis. Results: The experts suggested the use of existing indicators as well as new indicators. Thirty-nine indicators suggested by the experts are currently in use in Portugal. The assessment of the number of clinical acts performed, the number of administrative acts, and evaluation of the clinical demographic profile achieved a high degree of consensus. The expert panel suggested fifty new indicators. Five categories of these new indicators had a high degree of consensus, and three categories had a low degree of consensus. Conclusion: The expert panel recommended that performance indicators of practice management should first assess the quantity of clinical and administrative activities undertaken. These indicators must take into account the human and financial resources available to the clinic and its demographic context

Reação de cultivares comerciais e clones experimentais de Ipomoea batatas L. em campo naturalmente infestado com Meloidogyne javanica e M. incognita raça 1.

O objetivo deste trabalho foi avaliar a reação de cultivares e clones experimentais de batata-doce ao nematoide-das-galhas, a fim de incrementar a variabilidade de materiais disponíveis no mercado com resistência a este patógeno de solo.Resumo