Digital X-ray camera for quality evaluation three-dimensional topographic reconstruction of single crystals of biological macromolecules

The present invention provides a digital topography imaging system for determining the crystalline structure of a biological macromolecule, wherein the system employs a charge coupled device (CCD) camera with antiblooming circuitry to directly convert x-ray signals to electrical signals without the use of phosphor and measures reflection profiles from the x-ray emitting source after x-rays are passed through a sample. Methods for using said system are also provided

Letter from Edward Bellamy, Chicopee Falls, Massachusetts, to Anne Whitney, Boston, Massachusetts, 1889 March 15

https://repository.wellesley.edu/whitney_correspondence/2444/thumbnail.jp

Letter from Edward Bellamy, Chicopee Falls, Massachusetts, to Anne Whitney, Boston, Massachusetts, 1889 February 28

https://repository.wellesley.edu/whitney_correspondence/2443/thumbnail.jp

In hundert yohr arum

https://www.ester.ee/record=b5459831*es

Letter from Edward Bellamy, Chicopee Falls, Massachusetts, to Anne Whitney, Boston, Massachusetts, 1890 April 25

https://repository.wellesley.edu/whitney_correspondence/2447/thumbnail.jp

Manuel Sacristán at the Onset of Ecological Marxism after Stalinism

Thirty-one years ago, in 1985, Manuel Sacristán died in Barcelona at the age of 59. After the publication in 2014 of a volume with some of his writings translated into English (Llorente 2014), it is time to help non-Spanish-speaking readers to know more about him. Yet it is not easy to explain to generations born after the fall of the Berlin Wall in 1989 that Manuel Sacristán was a most important Marxist philosopher and at the same time one of the few pioneers introducing political ecology and antinuclear peace movement during the last quarter of the 20th century in Spain. Many people believe that Marxism, environmentalism and pacifism are views that exclude each other. Most of what has been said and done on behalf of Marxism since Stalin took over the leadership of the Communist Party of the USSR in the 1930s, up to its dissolution in 1991, contributes to sustaining this belief. The fast industrialization of the Old Russian Empire undertaken by the Soviet State was nowhere near taking into account ecological sustainability. Its socio-environmental impact turned out to be comparable or even worse than the ones caused by capitalist industrialization

Quantitative Evidence for Revising the Definition of Primary Graft Dysfunction after Lung Transplant

RATIONALE: Primary graft dysfunction (PGD) is a form of acute lung injury that occurs after lung transplantation. The definition of PGD was standardized in 2005. Since that time, clinical practice has evolved, and this definition is increasingly used as a primary endpoint for clinical trials; therefore, validation is warranted. OBJECTIVES: We sought to determine whether refinements to the 2005 consensus definition could further improve construct validity. METHODS: Data from the Lung Transplant Outcomes Group multicenter cohort were used to compare variations on the PGD definition, including alternate oxygenation thresholds, inclusion of additional severity groups, and effects of procedure type and mechanical ventilation. Convergent and divergent validity were compared for mortality prediction and concurrent lung injury biomarker discrimination. MEASUREMENTS AND MAIN RESULTS: A total of 1,179 subjects from 10 centers were enrolled from 2007 to 2012. Median length of follow-up was 4 years (interquartile range = 2.4-5.9). No mortality differences were noted between no PGD (grade 0) and mild PGD (grade 1). Significantly better mortality discrimination was evident for all definitions using later time points (48, 72, or 48-72 hours; P < 0.001). Biomarker divergent discrimination was superior when collapsing grades 0 and 1. Additional severity grades, use of mechanical ventilation, and transplant procedure type had minimal or no effect on mortality or biomarker discrimination. CONCLUSIONS: The PGD consensus definition can be simplified by combining lower PGD grades. Construct validity of grading was present regardless of transplant procedure type or use of mechanical ventilation. Additional severity categories had minimal impact on mortality or biomarker discrimination

Disseminated Langerhans cell histiocytosis presenting as oesophageal disease in a cat.

Case summaryAn 11-year-old female spayed domestic shorthair cat was referred with a 2-month history of ptyalism, hyporexia and weight loss. Physical examination revealed reduced body condition score (2/9) and decreased skin turgor. Laboratory abnormalities included mild erythrocytosis, elevated creatine kinase, hypercobalaminaemia and hypofolataemia. CT of the head and abdominal ultrasonography were within normal limits. Gastroesophagoscopy revealed mucosal ulceration and possible stenosis of the distal oesophagus. Thoracic radiographs and iodine oesophagram showed a soft tissue opacity in the caudodorsal thorax compatible with a parietal oesophageal mass causing luminal stenosis or an extra-parietal mass causing ventral displacement and compression of the oesophagus. Pulmonary nodules were observed in the cranial lung lobes. CT of the thorax confirmed the oesophageal origin of the mass and the presence of pulmonary nodules scattered throughout the lung parenchyma. The patient was euthanased given the imaging findings and perceived guarded prognosis. Post-mortem examination revealed multifocal nodular lesions affecting the oesophagus, lungs, kidneys and pancreas. Histopathological examination identified atypical round cells characterised by eosinophilic cytoplasm and pale nuclei with prominent nuclear grooves, compatible with neoplastic histiocytic cells. Immunohistochemistry revealed strong expression for CD18, Iba-1 and vimentin. Transmission electron microscopy demonstrated intracytoplasmic organelles consistent with Birkbeck granules of Langerhans cell origin in lesional histiocytes. These findings were compatible with a diagnosis of disseminated Langerhans cell histiocytosis.Relevance and novel informationTo our knowledge, this is the first report of disseminated Langerhans cell histiocytosis with oesophageal involvement in a cat

Implementing Evidence-Based Practice Education in Social Work: A Transdisciplinary Approach

Evidence based practice (EBP) is reflected in social work publications, accreditation standards, research, and funding opportunities. However, implementing EBP in social work practice and education has proven challenging, highlighting the need for additional resources. This paper describes the Transdisciplinary Model of EBP, a model based on advances in EBP across health disciplines including social work and its application to the development of an online EBP training portal. Utility of the Transdiciplinary Model and a training portal for social work education is discussed. Also included is a description of the training modules, the Council on Social Work Education Educational Policy and Accreditation Standards competencies reflected in the modules, and a case example using the modules in a master s of social work course

Safety and immunogenicity of varied doses of R21/Matrix-M™ vaccine at three years follow-up: A phase 1b age de-escalation, dose-escalation trial in adults, children, and infants in Kilifi-Kenya

BackgroundFalciparum malaria remains a global health problem. Two vaccines, based on the circumsporozoite antigen, are available. RTS, S/AS01 was recommended for use in 2021 following the advice of the World Health Organisation (WHO) Strategic Advisory Group of Experts (SAGE) on Immunization and WHO Malaria Policy Advisory Group (MPAG). It has since been pre-qualified in 2022 by the WHO. R21 is similar to RTS, S/AS01, and recently licensed in Nigeria, Ghana and Burkina Faso following Phase 3 trial results.MethodsWe conducted a Phase 1b age de-escalation, dose escalation bridging study after a change in the manufacturing process for R21. We recruited healthy adults and children and used a three dose primary vaccination series with a booster dose at 1-2 years. Variable doses of R21 and adjuvant (Matrix-M ™) were administered at 10µgR21/50 µg Matrix-M™, 5µgR21/25µg Matrix-M™ and 5µgR21/50µg Matrix-M™ to 20 adults, 20 children, and 51 infants.ResultsSelf-limiting adverse events were reported relating to the injection site and mild systemic symptoms. Two serious adverse events were reported, neither linked to vaccination. High levels of IgG antibodies to the circumsporozoite antigen were induced, and geometric mean titres in infants, the target group, were 1.1 (0.9 to 1.3) EU/mL at day 0, 10175 (7724 to 13404) EU/mL at day 84 and (following a booster dose at day 421) 6792 (5310 to 8687) EU/mL at day 456.ConclusionR21/Matrix-M™ is safe, and immunogenic when given at varied doses with the peak immune response seen in infants 28 days after a three dose primary vaccination series given four weeks apart. Antibody responses were restored 28 days after a 4 th dose given one year post a three dose primary series in the young children and infants.RegistrationClinicaltrials.gov (NCT03580824; 9 th of July 2018; Pan African Clinical Trials Registry (PACTR202105682956280; 17 th May 2021)