Minimally invasive monitoring of the central nervous system

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Environmental cleaning mission Bioconversion of oxidatively fragmented polyethylene plastic waste to value-added copolyesters

The innovative recycling method, we are proposing, relies upon the controlled oxidative fragmentation of waste LDPE plastic to the inexpensive substrates for future sustainable production of PHAs with the aid of Cupriavidus necator. LDPE oxidized fragments (PE-F) were obtained from the re-engineering LDPE film by means of pro-oxidant/pro-degradant additives, followed by treatment under natural UV light. Cupriavidus necator was grown in either tryptone soya broth (TSB) or basal salt medium (BSM) supplemented with PE-F for 48 h. PHA production was higher in TSB supplemented with PE-F (29%) than in TSB alone (only 0.6%). No PHA was detected in either BSM alone or BSM supplemented with PE-F. The recovered PHA was characterized using GPC, NMR, and electrospray ionization tandem mass spectrometry (ESI-MS/MS). These analytical tools applied confirmed that the resulting PHA was a terpolymer having an average molar mass of 624 kg/mol and consisting of 3-hydroxybutyrate (HB), 3-hydroxyvalerates (HV) and 3-hydroxyhexanoate (HH) co-monomer units randomly distributed along the chain backbone

Observations and Recommendations for the Calibration of Landsat 8 OLI and Sentinel 2 MSI for Improved Data Interoperability

Combining data from multiple sensors into a single seamless time series, also known as data interoperability, has the potential for unlocking new understanding of how the Earth functions as a system. However, our ability to produce these advanced data sets is hampered by the differences in design and function of the various optical remote-sensing satellite systems. A key factor is the impact that calibration of these instruments has on data interoperability. To address this issue, a workshop with a panel of experts was convened in conjunction with the Pecora 20 conference to focus on data interoperability between Landsat and the Sentinel 2 sensors. Four major areas of recommendation were the outcome of the workshop. The first was to improve communications between satellite agencies and the remote-sensing community. The second was to adopt a collections-based approach to processing the data. As expected, a third recommendation was to improve calibration methodologies in several specific areas. Lastly, and the most ambitious of the four, was to develop a comprehensive process for validating surface reflectance products produced from the data sets. Collectively, these recommendations have significant potential for improving satellite sensor calibration in a focused manner that can directly catalyze efforts to develop data that are closer to being seamlessly interoperable

Liquefied Bleed for Stability and Efficiency of High Speed Inlets

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Mastering Cryogenic Propellants

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Landsat-7 ETM+: 12 years On-Orbit Reflective-Band Radiometric Performance

The Landsat-7 ETM+ sensor has been operating on orbit for more than 12 years and characterizations of its performance have been ongoing over this period. In general, the radiometric performance of the instrument has been remarkably stable: (1) Noise performance has degraded by 2% or less overall, with a few detectors displaying step changes in noise of 2% or less, (2) Coherent noise frequencies and magnitudes have generally been stable, though the within-scan amplitude variation of the 20kHz noise in bands 1 and 8 disappeared with the failure of the scan line corrector and a new similar frequency noise (now about 18kHz) has appeared in two detectors in band 5 and increased in magnitude with time, (3) Bias stability has been better than 0.25 DN out of a normal value of 15 DN in high gain, (4) Relative gains, the differences in response between the detectors in the band, have generally changed by 0.1% or less over the mission, with the exception of a few detectors with a step response change of 1% or less and (5) Gain stability averaged across all detectors in a band, which is related to the stability of the absolute calibration, has been more stable than the techniques used to measure it. Due to the inability to confirm changes in the gain (beyond a few detectors that have been corrected back to the band average), ETM+ reflective band data continues to be calibrated with the pre-launch measured gains. In the worst case some bands may have changed as much as 2% in uncompensated absolute calibration over the 12 years

Mapping a multiplexed zoo of mRNA expression

In situ hybridization methods are used across the biological sciences to map mRNA expression within intact specimens. Multiplexed experiments, in which multiple target mRNAs are mapped in a single sample, are essential for studying regulatory interactions, but remain cumbersome in most model organisms. Programmable in situ amplifiers based on the mechanism of hybridization chain reaction (HCR) overcome this longstanding challenge by operating independently within a sample, enabling multiplexed experiments to be performed with an experimental timeline independent of the number of target mRNAs. To assist biologists working across a broad spectrum of organisms, we demonstrate multiplexed in situ HCR in diverse imaging settings: bacteria, whole-mount nematode larvae, whole-mount fruit fly embryos, whole-mount sea urchin embryos, whole-mount zebrafish larvae, whole-mount chicken embryos, whole-mount mouse embryos and formalin-fixed paraffin-embedded human tissue sections. In addition to straightforward multiplexing, in situ HCR enables deep sample penetration, high contrast and subcellular resolution, providing an incisive tool for the study of interlaced and overlapping expression patterns, with implications for research communities across the biological sciences

Mass spectrometry reveals molecular structure of polyhydroxyalkanoates attained by bioconversion of oxidized polypropylene waste fragments

This study investigated the molecular structure of the polyhydroxyalkanoate (PHA) produced via a microbiological shake flask experiment utilizing oxidized polypropylene (PP) waste as an additional carbon source. The bacterial strain Cupriavidus necator H16 was selected as it is non-pathogenic, genetically stable, robust, and one of the best known producers of PHA. Making use of PHA oligomers, formed by controlled moderate-temperature degradation induced by carboxylate moieties, by examination of both the parent and fragmentation ions, the ESI-MS/MS analysis revealed the 3-hydroxybutyrate and randomly distributed 3-hydroxyvalerate as well as 3-hydroxyhexanoate repeat units. Thus, the bioconversion of PP solid waste to a value-added product such as PHA tert-polymer was demonstrated.This research was funded by the Research Investment Fund, University of Wolverhampton, Faculty of Science and Engineering, UK. This work was also partially supported the European Regional Development Fund Project EnTRESS No 01R16P00718 and the PELARGODONT Project UM0-2016/22/Z/STS/00692 financed under the M-ERA.NET 2 Program of Horizon 2020.Published onlin

Sauver Byzance de la barbarie du monde

Il volume ripercorre la trattazione del mitema di Bisanzio nella letteratura francese e francofona dalle origini ai giorni nostr

Mapping a multiplexed zoo of mRNA expression

In situ hybridization methods are used across the biological sciences to map mRNA expression within intact specimens. Multiplexed experiments, in which multiple target mRNAs are mapped in a single sample, are essential for studying regulatory interactions, but remain cumbersome in most model organisms. Programmable in situ amplifiers based on the mechanism of hybridization chain reaction (HCR) overcome this longstanding challenge by operating independently within a sample, enabling multiplexed experiments to be performed with an experimental timeline independent of the number of target mRNAs. To assist biologists working across a broad spectrum of organisms, we demonstrate multiplexed in situ HCR in diverse imaging settings: bacteria, whole-mount nematode larvae, whole-mount fruit fly embryos, whole-mount sea urchin embryos, whole-mount zebrafish larvae, whole-mount chicken embryos, whole-mount mouse embryos and formalin-fixed paraffin-embedded human tissue sections. In addition to straightforward multiplexing, in situ HCR enables deep sample penetration, high contrast and subcellular resolution, providing an incisive tool for the study of interlaced and overlapping expression patterns, with implications for research communities across the biological sciences