Caracterização do pão regional do distrito de Viseu e de Pão São

Dissertação de Mestrado em Qualidade e Tecnologia AlimentarO objectivo deste estudo visa a caracterização de diferentes amostras de pão Regional e pão São, procurando encontrar semelhanças e identificar os elementos diferenciadores dos mesmos. As amostras de pão Regional foram recolhidas no distrito de Viseu: Carregal do Sal, Cabanas de Viriato, Santar, Viseu, Mangualde, São Pedro do Sul e São Cipriano. No caso do pão São as amostras foram fornecidas pela empresa produtora, Fábrica do Pão localizada em Seia. Procedeu-se à caracterização física, química e sensorial, tendo sido avaliadas as diferentes propriedades em termos de média e desvio padrão das réplicas efectuadas. Posteriormente, procedeu-se a uma análise estatística, para comparação de médias e avaliação da significância das diferenças, tendo para tal sido usado o teste de Tuckey HSD. Foram ainda calculados os coeficientes de correlação de Pearson. Os resultados foram analisados recorrendo ao software SPSS para o Windows versão 19.0 e Statistic versão 6., sendo realizado o teste de análise de variância "one-way" (ANOVA). Numa fase posterior, os valores obtidos para as características do pão foram sujeitos a uma análise de componentes principais (PCA) e uma análise de Cluster. Em relação aos parâmetros químicos analisados no pão Regional, as amostras apresentaram algumas semelhanças e algumas diferenças, assim como também a nível físico e sensorial. No entanto, no que respeita à análise sensorial, as amostras foram, de modo geral, percepcionadas da mesma forma pelos provadores, não sendo detectadas diferenças significativas. Ainda assim, em termos de apreciação global a amostra preferida foi a da pastelaria Flor de Cabanas. Em relação aos parâmetros químicos, físicose sensoriais do pão São, as amostras mostraram-se semelhantes entre si, o que pode ser explicado por terem basicamente a mesma constituição em termos de ingredientes. Contudo, será de assinalar algumas diferenças em termos de fibra, hidratos de carbono, proteína e cloretos, devido à presença de farinha de tremoço numa das amostras. Quando comparadas as amostras de pão Regional com as de pão São, verificou-se que estas se apresentaram bastante diferentes, quer em termos químicos, físicos ou nível sensorial. Foi possível observar que ao nível sensorial os pães Regionais foram os preferidos pelos provadores.ABSTRACT: The aim of this study is the characterization of different samples of regional bread and healthy bread, trying to identify the similarities and contrasting elements in them. The regional bread samples were collected in the District of Viseu: Carregal do Sal, Cabanas de Viriato, Santar, Viseu, São Pedro do Sul and São Cipriano. The healthy bread samples were provided by the production company "Fábrica do Pão", located in Seia. The physical, chemical and sensory characterization was undertaken, having been avaluted the different proprerties in terms of mean value and standard deviation of the replicas made. Later, we proceeded to a statistical analysis for comparison of means and assessing the significance of the differences, having been used for this the Tukey HSD test. We also calculated the Pearson correlation coefficients. The results were analyzaed using the SPSS software version 19.0 for Windows and Statistica version 6, having been performed an analysis of variance "one way"(ANOVA). At a later stage, the values obtained for the characteristics of bread were subjected to principal components analysis (PCA) and cluster analysis. Regardind the chemical parameters analyzed in the regional bread, the samples showed some similarities and differences, as well as regarding the physical and sensory parameters. However, concerning the sensory analysis, the samples were generally perceived by the panelists in the same way, and no significant differences were observed. Still, in terms of the same way preferred by the panelists the sample from pastry "Flor de Cabanas". With respect to chemical, physical and sensory parameters of the healthy bread, the samples were similar, which can be explained by having basically the same constitution in terms of ingredients. However, there can be noted some differences in terms of fiber, carbohydrates, protein and chlorides, due to the presence of lupine flour in one sample. When comparing the regional bread samples with the healthy bread ones, it was found that they quite different, either at the chemical, physical or sensory levels. It was observed that at the sensory level the regional breads were those preferred by the tasters

Extracellular Vesicles From Liver Progenitor Cells Downregulates Fibroblast Metabolic Activity and Increase the Expression of Immune-Response Related Molecules

Extracellular vesicles (EVs) mediate cell-to-cell crosstalk whose content can induce changes in acceptor cells and their microenvironment. MLP29 cells are mouse liver progenitor cells that release EVs loaded with signaling cues that could affect cell fate. In the current work, we incubated 3T3-L1 mouse fibroblasts with MLP29-derived EVs, and then analyzed changes by proteomics and transcriptomics. Results showed a general downregulation of protein and transcript expression related to proliferative and metabolic routes dependent on TGF-beta. We also observed an increase in the ERBB2 interacting protein (ERBIN) and Cxcl2, together with an induction of ribosome biogenesis and interferon-related response molecules, suggesting the activation of immune system signaling

PDGF-BB serum levels are decreased in adult onset Pompe patients

Adult onset Pompe disease is a genetic disorder characterized by slowly progressive skeletal and respiratory muscle weakness. Symptomatic patients are treated with enzymatic replacement therapy with human recombinant alfa glucosidase. Motor functional tests and spirometry are commonly used to follow patients up. However, a serological biomarker that correlates with the progression of the disease could improve follow-up. We studied serum concentrations of TGFβ, PDGF-BB, PDGF-AA and CTGF growth factors in 37 adult onset Pompe patients and 45 controls. Moreover, all patients performed several muscle function tests, conventional spirometry, and quantitative muscle MRI using 3-point Dixon. We observed a statistically significant change in the serum concentration of each growth factor in patients compared to controls. However, only PDGF-BB levels were able to differentiate between asymptomatic and symptomatic patients, suggesting its potential role in the follow-up of asymptomatic patients. Moreover, our results point to a dysregulation of muscle regeneration as an additional pathomechanism of Pompe disease

Relación entre las prácticas tecnológicas de manejo, la producción y su asociación con las épocas del año en el sistema de doble propósito del trópico mexicano

El objetivo de este estudio fue describir y evaluar la relación ent re las prácticas tecnológicas de manejo de hatos de bovinos de doble propósito y su productividad. Además de su asociación con las diferentes épocas del año. Para esto se analizaron los registros mensuales de las prácticas tecnológicas y variables producti vas de treinta unidades de producción de los estados de Tabasco, Chiapas y Sinaloa durante un año. Mediante la técnica de Correlaciones Canónicas se estimó la relación entre las prácticas tecnológicas: suplementación, sanidad, prevención y control de enfer medades y manejo de praderas (X) con variables productivas: producción de leche, porcentaje de vacas en ordeño y número de crías (Y). El análisis de correspondencia midió la asociación del uso de las prácticas tecnológicas y la época del año. Los resultado s mostraron tres correlaciones canónicas, la primera fue de 3.33 y significativa, con una variabilidad observada del 89% y las prácticas tecnológicas que mejor explican las variables productivas son la suplementación, sanidad, prevención y control de enfer medades. El análisis de correspondencia mostró que los productores de Tabasco y Chiapas varían las prácticas tecnológicas de manejo del hato de acuerdo a la época de año (lluvia, seca y transición) mientras los productores de Sinaloa las realizan durante t odo el año. El uso de las prácticas tecnológicas en las unidades de producción influye en las variables productivas, principalmente en la producción de leche

Improved integrative analysis of the thiol redox proteome using filter-aided sample preparation

Changes in the oxidation state of protein Cys residues are involved in cell signalling and play a key role in a variety of pathophysiological states. We had previously developed GELSILOX, an in-gel method that enables the large-scale, parallel analysis of dynamic alterations to the redox state of Cys sites and protein abundance changes. Here we present FASILOX, a further development of the GELSILOX approach featuring: i) significantly increased peptide recovery, ii) enhanced sensitivity for the detection of Cys oxidative alterations, and iii) streamlined workflow that results in shortened assay duration. In mitochondria isolated from the adipose tissue of obese, diabetic patients, FASILOX revealed a sexually dimorphic trait of Cys oxidation involving mainly mitochondrial oxidative phosphorylation complexes. These results provide the first evidence for a decreased efficiency in the antioxidant response of men as compared to women.This study was supported by competitive grants from the Spanish Ministry of Science, Innovation and Universities (BIO2015-67580-P, PGC2018-097019-B-I00 and RTI2018-093864-B-100),the Ministry of Economy, Industry and Competitiveness (BFU2016-80006-P) through the Carlos III Institute of Health-Fondo de InvestigaciónSanitaria grant PRB3 (IPT17/0019 -ISCIII-SGEFI / ERDF, ProteoRed), the Fundació MaratóTV3 (grant 122/C/2015) and “la Caixa” Banking Foundation (project code HR17-00247). The CNIC is supported by the Instituto de Salud Carlos III (ISCIII), the Ministerio de Ciencia, Innovación y Universidades (MCNU) and the Pro CNIC Foundation, and is a Severo Ochoa Center of Excellence (SEV-2015-0505)S