Velocity-space sensitivity of the time-of-flight neutron spectrometer at JET

The velocity-space sensitivities of fast-ion diagnostics are often described by so-called weight functions. Recently, we formulated weight functions showing the velocity-space sensitivity of the often dominant beam-target part of neutron energy spectra. These weight functions for neutron emission spectrometry (NES) are independent of the particular NES diagnostic. Here we apply these NES weight functions to the time-of-flight spectrometer TOFOR at JET. By taking the instrumental response function of TOFOR into account, we calculate time-of-flight NES weight functions that enable us to directly determine the velocity-space sensitivity of a given part of a measured time-of-flight spectrum from TOFOR

Relationship of edge localized mode burst times with divertor flux loop signal phase in JET

A phase relationship is identified between sequential edge localized modes (ELMs) occurrence times in a set of H-mode tokamak plasmas to the voltage measured in full flux azimuthal loops in the divertor region. We focus on plasmas in the Joint European Torus where a steady H-mode is sustained over several seconds, during which ELMs are observed in the Be II emission at the divertor. The ELMs analysed arise from intrinsic ELMing, in that there is no deliberate intent to control the ELMing process by external means. We use ELM timings derived from the Be II signal to perform direct time domain analysis of the full flux loop VLD2 and VLD3 signals, which provide a high cadence global measurement proportional to the voltage induced by changes in poloidal magnetic flux. Specifically, we examine how the time interval between pairs of successive ELMs is linked to the time-evolving phase of the full flux loop signals. Each ELM produces a clear early pulse in the full flux loop signals, whose peak time is used to condition our analysis. The arrival time of the following ELM, relative to this pulse, is found to fall into one of two categories: (i) prompt ELMs, which are directly paced by the initial response seen in the flux loop signals; and (ii) all other ELMs, which occur after the initial response of the full flux loop signals has decayed in amplitude. The times at which ELMs in category (ii) occur, relative to the first ELM of the pair, are clustered at times when the instantaneous phase of the full flux loop signal is close to its value at the time of the first ELM

Involvement of phosphoinositide 3-kinase in insulin stimulation of MAP-kinase and phosphorylation of protein kinase-B in human skeletal muscle: implications for glucose metabolism

Isolated skeletal muscle from healthy individuals was used to evaluate the role of phosphoinositide 3-kinase (PI3-kinase) in insulin signalling pathways regulating mitogen activated protein kinase (MAP-kinase) and protein kinase-B and to investigate whether MAP-kinase was involved in signalling pathways regulating glucose metabolism. Insulin stimulated glycogen synthase activity (approximate to 1.7 fold), increased 3-o-methylglucose transport into human skeletal muscle strips (approximate to 2 fold) and stimulated phosphorylation of the p42 ERK-2 isoform of MAP-kinase. This phosphorylation of p42 ERK2 was not blocked by the PI 3-kinase inhibitors LY294002 and wortmannin although it was blocked by the MAP-kinase kinase (MEK) inhibitor PD 95059. However, PD98059 (up to 20 mu mol/l) did not block insulin activation of glycogen synthase or stimulation of 3-o-methylglucose transport. Wortmannin and LY294002 did block insulin stimulation of protein kinase-B (PKB) phosphorylation and stimulation of 3-o-methylglucose transport was inhibited by wortmannin (IC50 approximate to 100 nmol/l). These results indicate that MAP-kinase is activated by insulin in human skeletal muscle by a PI 3-kinase independent pathway, Furthermore this activation is not necessary for insulin stimulation of glucose transport or activation of glycogen synthase in this tissue

Beware of your Cre-Ation: lacZ expression impairs neuronal integrity and hippocampus-dependent memory.

Expression of the lacZ-sequence is a widely used reporter-tool to assess the transgenic and/or transfection efficacy of a target gene in mice. Once activated, lacZ is permanently expressed. However, protein accumulation is one of the hallmarks of neurodegenerative diseases. Furthermore, the protein product of the bacterial lacZ gene is ß-galactosidase, an analog to the mammalian senescence-associated ß-galactosidase, a molecular marker for aging. Therefore we studied the behavioral, structural and molecular consequences of lacZ expression in distinct neuronal sub-populations. lacZ expression in cortical glutamatergic neurons resulted in severe impairments in hippocampus-dependent memory accompanied by marked structural alterations throughout the CNS. In contrast, GFP expression or the expression of the ChR2/YFP fusion product in the same cell populations did not result in either cognitive or structural deficits. GABAergic lacZ expression caused significantly decreased hyper-arousal and mild cognitive deficits. Attenuated structural and behavioral consequences of lacZ expression could also be induced in adulthood, and lacZ transfection in neuronal cell cultures significantly decreased their viability. Our findings provide a strong caveat against the use of lacZ reporter mice for phenotyping studies and point to a particular sensitivity of the hippocampus formation to detrimental consequences of lacZ expression

Note on arXiv:2005.05301, 'Preparation of the Neutrino-4 experiment on search for sterile neutrino and the obtained results of measurements'

We comment on the claimed observation [arXiv:arXiv:2005.05301] of sterile neutrino oscillations by the Neutrino-4 collaboration. Such a claim, which requires the existence of a new fundamental particle, demands a level of rigor commensurate with its impact. The burden lies with the Neutrino-4 collaboration to provide the information necessary to prove the validity of their claim to the community. In this note, we describe aspects of both the data and analysis method that might lead to an oscillation signature arising from a null experiment and describe additional information needed from the Neutrino-4 collaboration to support the oscillation claim. Additionally, as opposed to the assertion made by the Neutrino-4 collaboration, we also show that the method of 'coherent summation' using the $L/E$ parameter produces similar results to the methods used by the PROSPECT and the STEREO collaborations

Impact of nitrogen seeding on confinement and power load control of a high-triangularity JET ELMy H-mode plasma with a metal wall

This paper reports the impact on confinement and power load of the high-shape 2.5MA ELMy H-mode scenario at JET of a change from an all carbon plasma facing components to an all metal wall. In preparation to this change, systematic studies of power load reduction and impact on confinement as a result of fuelling in combination with nitrogen seeding were carried out in JET-C and are compared to their counterpart in JET with a metallic wall. An unexpected and significant change is reported on the decrease of the pedestal confinement but is partially recovered with the injection of nitrogen.Comment: 30 pages, 16 figure

Note on arXiv:2005.05301, 'Preparation of the Neutrino-4 experiment on search for sterile neutrino and the obtained results of measurements'

We comment on the claimed observation [arXiv:arXiv:2005.05301] of sterile neutrino oscillations by the Neutrino-4 collaboration. Such a claim, which requires the existence of a new fundamental particle, demands a level of rigor commensurate with its impact. The burden lies with the Neutrino-4 collaboration to provide the information necessary to prove the validity of their claim to the community. In this note, we describe aspects of both the data and analysis method that might lead to an oscillation signature arising from a null experiment and describe additional information needed from the Neutrino-4 collaboration to support the oscillation claim. Additionally, as opposed to the assertion made by the Neutrino-4 collaboration, we also show that the method of 'coherent summation' using the $L/E$ parameter produces similar results to the methods used by the PROSPECT and the STEREO collaborations

Note on arXiv:2005.05301, 'Preparation of the Neutrino-4 experiment on search for sterile neutrino and the obtained results of measurements'

We comment on the claimed observation [arXiv:arXiv:2005.05301] of sterile neutrino oscillations by the Neutrino-4 collaboration. Such a claim, which requires the existence of a new fundamental particle, demands a level of rigor commensurate with its impact. The burden lies with the Neutrino-4 collaboration to provide the information necessary to prove the validity of their claim to the community. In this note, we describe aspects of both the data and analysis method that might lead to an oscillation signature arising from a null experiment and describe additional information needed from the Neutrino-4 collaboration to support the oscillation claim. Additionally, as opposed to the assertion made by the Neutrino-4 collaboration, we also show that the method of 'coherent summation' using the $L/E$ parameter produces similar results to the methods used by the PROSPECT and the STEREO collaborations