Development of Onchocerca volvulus in humanized NSG mice and detection of parasite biomarkers in urine and serum.

BACKGROUND: The study of Onchocerca volvulus has been limited by its host range, with only humans and non-human primates shown to be susceptible to the full life cycle infection. Small animal models that support the development of adult parasites have not been identified. METHODOLOGY/PRINCIPAL FINDINGS: We hypothesized that highly immunodeficient NSG mice would support the survival and maturation of O. volvulus and alteration of the host microenvironment through the addition of various human cells and tissues would further enhance the level of parasite maturation. NSG mice were humanized with: (1) umbilical cord derived CD34+ stem cells, (2) fetal derived liver, thymus and CD34+ stem cells or (3) primary human skeletal muscle cells. NSG and humanized NSG mice were infected with 100 O. volvulus infective larvae (L3) for 4 to 12 weeks. When necropsies of infected animals were performed, it was observed that parasites survived and developed throughout the infection time course. In each of the different humanized mouse models, worms matured from L3 to advanced fourth stage larvae, with both male and female organ development. In addition, worms increased in length by up to 4-fold. Serum and urine, collected from humanized mice for identification of potential biomarkers of infection, allowed for the identification of 10 O. volvulus-derived proteins found specifically in either the urine or the serum of the humanized O. volvulus-infected NSG mice. CONCLUSIONS/SIGNIFICANCE: The newly identified mouse models for onchocerciasis will enable the development of O. volvulus specific biomarkers, screening for new therapeutic approaches and potentially studying the human immune response to infection with O. volvulus

Nonlinear modal coupling in a high-stress doubly-clamped nanomechanical resonator

We present results from a study of the nonlinear intermodal coupling between different flexural vibrational modes of a single high-stress, doubly-clamped silicon nitride nanomechanical beam. The measurements were carried out at 100 mK and the beam was actuated using the magnetomotive technique. We observed the nonlinear behavior of the modes individually and also measured the coupling between them by driving the beam at multiple frequencies. We demonstrate that the different modes of the resonator are coupled to each other by the displacement induced tension in the beam, which also leads to the well known Duffing nonlinearity in doubly-clamped beams.Comment: 15 pages, 7 figure

Paper Session III-B - Space Technology Graduate Program for Engineers and Scientists

A Master of Science in Space Technology is offered to engineering and science graduates at the Spaceport Graduate Center which is an off-campus teaching center of the Florida Institute of Technology. Courses are offered in the evening at Kennedy Space Center and Patrick Air Force Base so that space professionals can continue their education. The goals of the program are to prepare students for broader responsibility and more rewarding careers in astronautics and space, as well as to contribute to improvement in defining and implementing national and international space goals. It has been endorsed by NASA, the USAF, and large aerospace companies. This paper describes an education program which removes interdisciplinary barriers in accepting a spectrum of students with different engineering and scientific backgrounds. Topics addressed include course curricula, the composition of the students and faculty, program successes and challenges, as well as plans for the future. Emphasis is given to the progress that has been made since Dr. Angelo described the program to the 23rd Space Congress in 1986. Over 70 students have graduated with an MS hi Space Technology and are advancing in their careers. The faculty has been expanded to include a full-time program chairman and twenty adjunct professors, seven of these holding Ph D\u27s in physics or astronomy and eight holding Ph D\u27s in engineering disciplines. An important objective of this paper is to solicit inputs to make the program even more valuable. An Industry Advisory Council is being set-up for this purpose, consisting of the Chief Executive Officers of local aerospace companies and government. Future developments will include research activity in conjunction with the Space Research Institute and a Space Engineering supplement. It is fundamental that better understanding of the technical aspects of all phases of space activity will foster improved coordination among, and better decisions by, the future leaders of Space and Astronautics

Are infestations of Cymomelanodactylus killing Acropora cytherea in the Chagos archipelago?

Associations between branching corals and infaunal crabs are well known, mostly due to the beneficial effects of Trapezia and Tetralia crabs in protecting host corals from crown-of-thorns starfish (e.g., Pratchett et al. 2000) and/or sedimentation (Stewart et al. 2006). These crabs are obligate associates of live corals and highly prevalent across suitable coral hosts, with 1–2 individuals per colony (Patton 1994). Cymo melanodactylus (Fig. 1) are also prevalent in branching corals, mostly Acropora, and are known to feed on live coral tissue, but are generally found in low abundance (<3 per colony) and do not significantly affect their host corals (e.g., Patton 1994). In the Chagos archipelago, however, infestations of Cymo melanodactylus were found on recently dead and dying colonies of Acropora cytherea

Infrared catastrophe and tunneling into strongly correlated electron systems: Exact solution of the x-ray edge limit for the 1D electron gas and 2D Hall fluid

In previous work we have proposed that the non-Fermi-liquid spectral properties in a variety of low-dimensional and strongly correlated electron systems are caused by the infrared catastrophe, and we used an exact functional integral representation for the interacting Green's function to map the tunneling problem onto the x-ray edge problem, plus corrections. The corrections are caused by the recoil of the tunneling particle, and, in systems where the method is applicable, are not expected to change the qualitative form of the tunneling density of states (DOS). Qualitatively correct results were obtained for the DOS of the 1D electron gas and 2D Hall fluid when the corrections to the x-ray edge limit were neglected and when the corresponding Nozieres-De Dominicis integral equations were solved by resummation of a divergent perturbation series. Here we reexamine the x-ray edge limit for these two models by solving these integral equations exactly, finding the expected modifications of the DOS exponent in the 1D case but finding no changes in the DOS of the 2D Hall fluid with short-range interaction. We also provide, for the first time, an exact solution of the Nozieres-De Dominicis equation for the 2D electron gas in the lowest Landau level.Comment: 6 pages, Revte