VLA neutral hydrogen imaging of compact groups

Images of the neutral hydrogen (H I) in the direction of the compact groups of galaxies, HCG 31, HCG 44, and HCG 79 are presented. The authors find in HCG 31 and HCG 79, emission contained within a cloud much larger than the galaxies as well as the entire group. The H I emission associated with HCG 44 is located within the individual galaxies but shows definite signs of tidal interactions. The authors imaged the distribution and kinematics of neutral hydrogen at the two extremes of group sizes represented in Hickson's sample. HCG 44 is at the upper limit while HCG 18, HCG 31, and HCG 79 are at the lower end. Although the number of groups that have been imaged is still very small, there may be a pattern emerging which describes the H I morphology of compact groups. The true nature of compact groups has been the subject of considerable debate and controversy. The most recent observational and theoretical evidence strongly suggests that compact groups are physically dense, dynamical systems that are in the process of merging into a single object (Williams and Rood 1987, Hickson and Rood 1988, Barnes 1989). The neutral hydrogen deficiency observed by Williams and Rood (1987) is consistent with a model in which frequent galactic collisions and interactions have heated some of the gas during the short lifetime of the group. The H I disks which are normally more extended than the luminous ones are expected to be more sensitive to collisions and to trace the galaxy's response to recent interactions. Very Large Array observations can provide in most cases the spatial resolution needed to confirm the dynamical interactions in these systems

Surface pressure measurements at two tips of a model helicopter rotor in hover

Surface pressures were measured near the tip of a hovering single-bladed model helicopter rotor with two tip shapes. The rotor had a constant-chord, untwisted blade with a square, flat tip which could be modified to a body-of-revolution tip. Pressure measurements were made on the blade surface along the chordwise direction at six radial stations outboard of the 94 percent blade radius. Data for each blade tip configuration were taken at blade collective pitch angles of 0, 6.18 and 11.4 degrees at a Reynolds number of 736,000 and a Mach number of 0.25 both based on tip speed. Chordwise pressure distributions and constant surface pressure contours are presented and discussed

The Infrared Properties of Submillimeter Galaxies: Clues From Ultra-Deep 70 Micron Imaging

We present 70 micron properties of submillimeter galaxies (SMGs) in the Great Observatories Origins Deep Survey (GOODS) North field. Out of thirty submillimeter galaxies (S_850 > 2 mJy) in the central GOODS-N region, we find two with secure 70 micron detections. These are the first 70 micron detections of SMGs. One of the matched SMGs is at z ~ 0.5 and has S_70/S_850 and S_70/S_24 ratios consistent with a cool galaxy. The second SMG (z = 1.2) has infrared-submm colors which indicate it is more actively forming stars. We examine the average 70 micron properties of the SMGs by performing a stacking analysis, which also allows us to estimate that S_850 > 2 mJy SMGs contribute 9 +- 3% of the 70 micron background light. The S_850/S_70 colors of the SMG population as a whole is best fit by cool galaxies, and because of the redshifting effects these constraints are mainly on the lower z sub-sample. We fit Spectral Energy Distributions (SEDs) to the far-infrared data points of the two detected SMGs and the average low redshift SMG (z_{median}= 1.4). We find that the average low-z SMG has a cooler dust temperature than local ultraluminous infrared galaxies (ULIRGs) of similar luminosity and an SED which is best fit by scaled up versions of normal spiral galaxies. The average low-z SMG is found to have a typical dust temperature T = 21 -- 33 K and infrared luminosity L_{8-1000 micron} = 8.0 \times 10^11 L_sun. We estimate the AGN contribution to the total infrared luminosity of low-z SMGs is less than 23%.Comment: Accepted by ApJ. 14 pages, 6 figures. Minor revisions 20th Dec 200

Conformational Dependence of a Protein Kinase Phosphate Transfer Reaction

Atomic motions and energetics for a phosphate transfer reaction catalyzed by the cAMP-dependent protein kinase (PKA) are calculated by plane-wave density functional theory, starting from structures of proteins crystallized in both the reactant conformation (RC) and the transition-state conformation (TC). In the TC, we calculate that the reactants and products are nearly isoenergetic with a 0.2 eV barrier; while phosphate transfer is unfavorable by over 1.2 eV in the RC, with an even higher barrier. With the protein in the TC, the motions involved in reaction are small, with only P$_\gamma$ and the catalytic proton moving more than 0.5 \AA. Examination of the structures reveals that in the RC the active site cleft is not completely closed and there is insufficient space for the phosphorylated serine residue in the product state. Together, these observations imply that the phosphate transfer reaction occurs rapidly and reversibly in a particular conformation of the protein, and that the reaction can be gated by changes of a few tenths of an \AA in the catalytic site.Comment: revtex4, 7 pages, 4 figures, to be submitted to Scienc

Holistic corpus-based dialectology

This paper is concerned with sketching future directions for corpus-based dialectology. We advocate a holistic approach to the study of geographically conditioned linguistic variability, and we present a suitable methodology, 'corpusbased dialectometry', in exactly this spirit. Specifically, we argue that in order to live up to the potential of the corpus-based method, practitioners need to (i) abandon their exclusive focus on individual linguistic features in favor of the study of feature aggregates, (ii) draw on computationally advanced multivariate analysis techniques (such as multidimensional scaling, cluster analysis, and principal component analysis), and (iii) aid interpretation of empirical results by marshalling state-of-the-art data visualization techniques. To exemplify this line of analysis, we present a case study which explores joint frequency variability of 57 morphosyntax features in 34 dialects all over Great Britain

Erioflorin stabilizes the tumor suppressor Pdcd4 by inhibiting its interaction with the E3-ligase β-TrCP1

Loss of the tumor suppressor Pdcd4 was reported for various tumor entities and proposed as a prognostic marker in tumorigenesis. We previously characterized decreased Pdcd4 protein stability in response to mitogenic stimuli, which resulted from p70S6K1-dependent protein phosphorylation, β-TrCP1-mediated ubiquitination, and proteasomal destruction. Following high-throughput screening of natural product extract libraries using a luciferase-based reporter assay to monitor phosphorylation-dependent proteasomal degradation of the tumor suppressor Pdcd4, we succeeded in showing that a crude extract from Eriophyllum lanatum stabilized Pdcd4 from TPA-induced degradation. Erioflorin was identified as the active component and inhibited not only degradation of the Pdcd4-luciferase-based reporter but also of endogenous Pdcd4 at low micromolar concentrations. Mechanistically, erioflorin interfered with the interaction between the E3-ubiquitin ligase β-TrCP1 and Pdcd4 in cell culture and in in vitro binding assays, consequently decreasing ubiquitination and degradation of Pdcd4. Interestingly, while erioflorin stabilized additional β-TrCP-targets (such as IκBα and β-catenin), it did not prevent the degradation of targets of other E3-ubiquitin ligases such as p21 (a Skp2-target) and HIF-1α (a pVHL-target), implying selectivity for β-TrCP. Moreover, erioflorin inhibited the tumor-associated activity of known Pdcd4- and IκBα-regulated αtranscription factors, that is, AP-1 and NF-κB, altered cell cycle progression and suppressed proliferation of various cancer cell lines. Our studies succeeded in identifying erioflorin as a novel Pdcd4 stabilizer that inhibits the interaction of Pdcd4 with the E3-ubiquitin ligase β-TrCP1. Inhibition of E3-ligase/target-protein interactions may offer the possibility to target degradation of specific proteins only as compared to general proteasome inhibition

Photochemistry of Furyl- and Thienyldiazomethanes: Spectroscopic Characterization of Triplet 3-Thienylcarbene

Photolysis (λ \u3e 543 nm) of 3-thienyldiazomethane (1), matrix isolated in Ar or N2 at 10 K, yields triplet 3-thienylcarbene (13) and α-thial-methylenecyclopropene (9). Carbene 13 was characterized by IR, UV/vis, and EPR spectroscopy. The conformational isomers of 3-thienylcarbene (s-E and s-Z) exhibit an unusually large difference in zero-field splitting parameters in the triplet EPR spectrum (|D/hc| = 0.508 cm–1, |E/hc| = 0.0554 cm–1; |D/hc| = 0.579 cm–1, |E/hc| = 0.0315 cm–1). Natural Bond Orbital (NBO) calculations reveal substantially differing spin densities in the 3-thienyl ring at the positions adjacent to the carbene center, which is one factor contributing to the large difference in D values. NBO calculations also reveal a stabilizing interaction between the sp orbital of the carbene carbon in the s-Z rotamer of 13 and the antibonding σ orbital between sulfur and the neighboring carbon—an interaction that is not observed in the s-E rotamer of 13. In contrast to the EPR spectra, the electronic absorption spectra of the rotamers of triplet 3-thienylcarbene (13) are indistinguishable under our experimental conditions. The carbene exhibits a weak electronic absorption in the visible spectrum (λmax = 467 nm) that is characteristic of triplet arylcarbenes. Although studies of 2-thienyldiazomethane (2), 3-furyldiazomethane (3), or 2-furyldiazomethane (4) provided further insight into the photochemical interconversions among C5H4S or C5H4O isomers, these studies did not lead to the spectroscopic detection of the corresponding triplet carbenes (2-thienylcarbene (11), 3-furylcarbene (23), or 2-furylcarbene (22), respectively)

The FIRST Bright Quasar Survey. II. 60 Nights and 1200 Spectra Later

We have used the VLA FIRST survey and the APM catalog of the POSS-I plates as the basis for constructing a new radio-selected sample of optically bright quasars. This is the first radio-selected sample that is competitive in size with current optically selected quasar surveys. Using only two basic criteria, radio-optical positional coincidence and optical morphology, quasars and BL Lacs can be identified with 60% selection efficiency; the efficiency increases to 70% for objects fainter than magnitude 17. We show that a more sophisticated selection scheme can predict with better than 85% reliability which candidates will turn out to be quasars. This paper presents the second installment of the FIRST Bright Quasar Survey with a catalog of 636 quasars distributed over 2682 square degrees. The quasar sample is characterized and all spectra are displayed. The FBQS detects both radio-loud and radio-quiet quasars out to a redshift z>3. We find a large population of objects of intermediate radio-loudness; there is no evidence in our sample for a bimodal distribution of radio characteristics. The sample includes ~29 broad absorption line quasars, both high and low ionization, and a number of new objects with remarkable optical spectra.Comment: 41 pages plus 39 gifs which contain all quasar spectra. Accepted for publication in the Astrophysical Journal Supplement Serie

Oligosaccharide and Glycoprotein Microarrays as Tools in HIV Glycobiology Glycan-Dependent gp120/Protein Interactions

AbstractDefining HIV envelope glycoprotein interactions with host factors or binding partners advances our understanding of the infectious process and provides a basis for the design of vaccines and agents that interfere with HIV entry. Here we employ carbohydrate and glycoprotein microarrays to analyze glycan-dependent gp120-protein interactions. In concert with new linking chemistries and synthetic methods, the carbohydrate arrays combine the advantages of microarray technology with the flexibility and precision afforded by organic synthesis. With these microarrays, we individually and competitively determined the binding profiles of five gp120 binding proteins, established the carbohydrate structural requirements for these interactions, and identified a potential strategy for HIV vaccine development