Filipinosenior high school teachers’ continuing professional development attitudes: Exploring the roles of perceived demand amid a national education reform

The professional development of teachers is an important component of quality standards for any educational system, as teachers’ engagement in continuing professional development (CPD) activities are related with aspects of professional commitment and satisfaction. In this study, we explore how perceived demands of a national educational reform in the Philippines are associatedwithdifferent indicators of senior high school teachers’ professional development.A survey of 289teachers recruited to teach senior high school for the first time in the Philippines indicated that perceived demand was not associated with CPD participation intentions,but thatperceived demand related to job requirement and to career planning had different associations with attitudes towards CPD. These different associations can be viewed as adaptive responding to the uncertainties in their changing work environment. The implications for conceptualizing the context of teachers’ professional development, and the external factors that strengthen or weaken teachers’ positive attitudes towards CPD are discussed

Multiplex quantitative PCR for single-reaction genetically modified (GM) plant detection and identification of false-positive GM plants linked to Cauliflower mosaic virus (CaMV) infection.

BACKGROUND:Most genetically modified (GM) plants contain a promoter, P35S, from the plant virus, Cauliflower mosaic virus (CaMV), and many have a terminator, TNOS, derived from the bacterium, Agrobacterium tumefaciens. Assays designed to detect GM plants often target the P35S and/or TNOS DNA sequences. However, because the P35S promoter is derived from CaMV, these detection assays can yield false-positives from non-GM plants infected by this naturally-occurring virus. RESULTS:Here we report the development of an assay designed to distinguish CaMV-infected plants from GM plants in a single multiplexed quantitative PCR (qPCR) reaction. Following initial testing and optimization via PCR and singleplex-to-multiplex qPCR on both plasmid and plant DNA, TaqMan qPCR probes with different fluorescence wavelengths were designed to target actin (a positive-control plant gene), P35S, P3 (a CaMV-specific gene), and TNOS. We tested the specificity of our quadruplex qPCR assay using different DNA extracts from organic watercress and both organic and GM canola, all with and without CaMV infection, and by using commercial and industrial samples. The limit of detection (LOD) of each target was determined to be 1% for actin, 0.001% for P35S, and 0.01% for both P3 and TNOS. CONCLUSIONS:This assay was able to distinguish CaMV-infected plants from GM plants in a single multiplexed qPCR reaction for all samples tested in this study, suggesting that this protocol is broadly applicable and readily transferrable to any interested parties with a qPCR platform

ALMA High-frequency Long Baseline Campaign in 2021: Highest Angular Resolution Submillimeter Wave Images for the Carbon-rich Star R Lep

The Atacama Large Millimeter/submillimeter Array (ALMA) was used in 2021 to image the carbon-rich evolved star R Lep in Bands 8-10 (397-908 GHz) with baselines up to 16 km. The goal was to validate the calibration, using band-to-band (B2B) phase referencing with a close phase calibrator J0504-1512, 1.2 deg from R Lep in this case, and the imaging procedures required to obtain the maximum angular resolution achievable with ALMA. Images of the continuum emission and the hydrogen cyanide (HCN) maser line at 890.8 GHz, from the J=10-9 transition between the (1110) and (0400) vibrationally excited states, achieved angular resolutions of 13, 6, and 5 mas in Bands 8-10, respectively. Self-calibration (self-cal) was used to produce ideal images as to compare with the B2B phase referencing technique. The continuum emission was resolved in Bands 9 and 10, leaving too little flux for self-cal of the longest baselines, so these comparisons are made at coarser resolution. Comparisons showed that B2B phase referencing provided phase corrections sufficient to recover 92%, 83%, and 77% of the ideal image continuum flux densities. The HCN maser was sufficiently compact to obtain self-cal solutions in Band 10 for all baselines (up to 16 km). In Band 10, B2B phase referencing as compared to the ideal images recovered 61% and 70% of the flux density for the HCN maser and continuum, respectively.Comment: 37 pages, 12 figures, 9 tables, accepted by ApJ (Aug 30, 2023

A low density of 0.8 g/cc for the Trojan binary asteroid 617 Patroclus

The Trojan population consists of two swarms of asteroids following the same orbit as Jupiter and located at the L4 and L5 Lagrange points of the Jupiter-Sun system (leading and following Jupiter by 60 degrees). The asteroid 617 Patroclus is the only known binary Trojan (Merline et al. 2001). The orbit of this double system was hitherto unknown. Here we report that the components, separated by 680 km, move around the system centre of mass, describing roughly a circular orbit. Using the orbital parameters, combined with thermal measurements to estimate the size of the components, we derive a very low density of 0.8 g/cc. The components of Patroclus are therefore very porous or composed mostly of water ice, suggesting that they could have been formed in the outer part of the solar system.Comment: 10 pages, 3 figures, 1 tabl

Progressive star formation in the young galactic super star cluster NGC 3603

Early release science observations of the cluster NGC3603 with the WFC3 on the refurbished HST allow us to study its recent star formation history. Our analysis focuses on stars with Halpha excess emission, a robust indicator of their pre-main sequence (PMS) accreting status. The comparison with theoretical PMS isochrones shows that 2/3 of the objects with Halpha excess emission have ages from 1 to 10 Myr, with a median value of 3 Myr, while a surprising 1/3 of them are older than 10 Myr. The study of the spatial distribution of these PMS stars allows us to confirm their cluster membership and to statistically separate them from field stars. This result establishes unambiguously for the first time that star formation in and around the cluster has been ongoing for at least 10-20 Myr, at an apparently increasing rate.Comment: 10 pages, 8 figures, accepted for publication in The Astrophysical Journa

Do female association preferences predict the likelihood of reproduction?

Sexual selection acting on male traits through female mate choice is commonly inferred from female association preferences in dichotomous mate choice experiments. However, there are surprisingly few empirical demonstrations that such association preferences predict the likelihood of females reproducing with a particular male. This information is essential to confirm association preferences as good predictors of mate choice. We used green swordtails (<i>Xiphophorus helleri</i>) to test whether association preferences predict the likelihood of a female reproducing with a male. Females were tested for a preference for long- or short-sworded males in a standard dichotomous choice experiment and then allowed free access to either their preferred or non-preferred male. If females subsequently failed to produce fry, they were provided a second unfamiliar male with similar sword length to the first male. Females were more likely to reproduce with preferred than non-preferred males, but for those that reproduced, neither the status (preferred/non-preferred) nor the sword length (long/short) of the male had an effect on brood size or relative investment in growth by the female. There was no overall preference based on sword length in this study, but male sword length did affect likelihood of reproduction, with females more likely to reproduce with long- than short-sworded males (independent of preference for such males in earlier choice tests). These results suggest that female association preferences are good indicators of female mate choice but that ornament characteristics of the male are also important