Activation of gene expression during hypersensitive response (HR) induced by auxin in the grapevine rootstock cultivar 'Börner'

The cultivar ‘Börner’ is one of the very few grape phylloxera (Daktulosphaira vitifoliae Fitch) resistant rootstocks commercially available. In contrast to tolerant or sensitive rootstocks, ‘Börner’ roots react to grape phylloxera attack with a hypersensitive response leading to necroses around the puncture sites. In this study, we identified genes differentially up-regulated during the HR. HR was chemically induced in root cells by the application of indol-acetic-acid (IAA). After a cDNA subtraction of induced and non-induced material, the subtracted cDNA-samples were also hybridized to Arabidopsis microarray chips to identify differentially expressed candidate genes. The microarray data were analyzed and differentially expressed genes were grouped into different functional categories, e.g. signal transduction pathways, transcription factors, defence associated genes. Primers were designed to target genes of interest putatively involved in the HR. So far, 38 ESTs induced in ‘Börner’ roots undergoing a HR have been sequenced and annotated.

Magnetic friction in Ising spin systems

A new contribution to friction is predicted to occur in systems with magnetic correlations: Tangential relative motion of two Ising spin systems pumps energy into the magnetic degrees of freedom. This leads to a friction force proportional to the area of contact. The velocity and temperature dependence of this force are investigated. Magnetic friction is strongest near the critical temperature, below which the spin systems order spontaneously. Antiferromagnetic coupling leads to stronger friction than ferromagnetic coupling with the same exchange constant. The basic dissipation mechanism is explained. If the coupling of the spin system to the heat bath is weak, a surprising effect is observed in the ordered phase: The relative motion acts like a heat pump cooling the spins in the vicinity of the friction surface.Comment: 4 pages, 4 figure

Cellular automata approach to three-phase traffic theory

The cellular automata (CA) approach to traffic modeling is extended to allow for spatially homogeneous steady state solutions that cover a two dimensional region in the flow-density plane. Hence these models fulfill a basic postulate of a three-phase traffic theory proposed by Kerner. This is achieved by a synchronization distance, within which a vehicle always tries to adjust its speed to the one of the vehicle in front. In the CA models presented, the modelling of the free and safe speeds, the slow-to-start rules as well as some contributions to noise are based on the ideas of the Nagel-Schreckenberg type modelling. It is shown that the proposed CA models can be very transparent and still reproduce the two main types of congested patterns (the general pattern and the synchronized flow pattern) as well as their dependence on the flows near an on-ramp, in qualitative agreement with the recently developed continuum version of the three-phase traffic theory [B. S. Kerner and S. L. Klenov. 2002. J. Phys. A: Math. Gen. 35, L31]. These features are qualitatively different than in previously considered CA traffic models. The probability of the breakdown phenomenon (i.e., of the phase transition from free flow to synchronized flow) as function of the flow rate to the on-ramp and of the flow rate on the road upstream of the on-ramp is investigated. The capacity drops at the on-ramp which occur due to the formation of different congested patterns are calculated.Comment: 55 pages, 24 figure

Two-lane traffic rules for cellular automata: A systematic approach

Microscopic modeling of multi-lane traffic is usually done by applying heuristic lane changing rules, and often with unsatisfying results. Recently, a cellular automaton model for two-lane traffic was able to overcome some of these problems and to produce a correct density inversion at densities somewhat below the maximum flow density. In this paper, we summarize different approaches to lane changing and their results, and propose a general scheme, according to which realistic lane changing rules can be developed. We test this scheme by applying it to several different lane changing rules, which, in spite of their differences, generate similar and realistic results. We thus conclude that, for producing realistic results, the logical structure of the lane changing rules, as proposed here, is at least as important as the microscopic details of the rules

From Capillary Condensation to Interface Localization Transitions in Colloid Polymer Mixtures Confined in Thin Film Geometry

Monte Carlo simulations of the Asakura-Oosawa (AO) model for colloid-polymer mixtures confined between two parallel repulsive structureless walls are presented and analyzed in the light of current theories on capillary condensation and interface localization transitions. Choosing a polymer to colloid size ratio of q=0.8 and studying ultrathin films in the range of D=3 to D=10 colloid diameters thickness, grand canonical Monte Carlo methods are used; phase transitions are analyzed via finite size scaling, as in previous work on bulk systems and under confinement between identical types of walls. Unlike the latter work, inequivalent walls are used here: while the left wall has a hard-core repulsion for both polymers and colloids, at the right wall an additional square-well repulsion of variable strength acting only on the colloids is present. We study how the phase separation into colloid-rich and colloid-poor phases occurring already in the bulk is modified by such a confinement. When the asymmetry of the wall-colloid interaction increases, the character of the transition smoothly changes from capillary condensation-type to interface localization-type. The critical behavior of these transitions is discussed, as well as the colloid and polymer density profiles across the film in the various phases, and the correlation of interfacial fluctuations in the direction parallel to the confining walls. The experimental observability of these phenomena also is briefly discussed.Comment: 36 pages, 15 figure

The 400d Galaxy Cluster Survey Weak Lensing Programme: I: MMT/Megacam Analysis of CL0030+2618 at z=0.50

The mass function of galaxy clusters at high redshifts is a particularly useful probe to learn about the history of structure formation and constrain cosmological parameters. We aim at deriving reliable masses for a high-redshift, high-luminosity sample of clusters of galaxies selected from the 400d survey of X-ray selected clusters. Here, we will focus on a particular object, CL0030+2618 at z=0.50 Using deep imaging in three passbands with the MEGACAM instrument at MMT, we show that MEGACAM is well-suited for measuring gravitational shear. We detect the weak lensing signal of CL0030+2618 at 5.8 sigma significance, using the aperture mass technique. Furthermore, we find significant tangential alignment of galaxies out to ~10 arcmin or >2r_200 distance from the cluster centre. The weak lensing centre of CL0030+2618 agrees with several X-ray measurements and the position of the brightest cluster galaxy. Finally, we infer a weak lensing virial mass of M_200=7.5 10^{14} M_sun for CL0030+2618. Despite complications by a tentative foreground galaxy group in the line of sight, the X-ray and weak lensing estimates for CL0030+2618 are in remarkable agreement. This study paves the way for the largest weak lensing survey of high-redshift galaxy clusters to date.Comment: 32 pages, 24 figures, submitted to Astronomy & Astrophysics; fixed some LaTeX issues, now 30 pages v3: Improved version accepted by Astronomy & Astrophysic

Salmonella Transiently Reside in Luminal Neutrophils in the Inflamed Gut

Enteric pathogens need to grow efficiently in the gut lumen in order to cause disease and ensure transmission. The interior of the gut forms a complex environment comprising the mucosal surface area and the inner gut lumen with epithelial cell debris and food particles. Recruitment of neutrophils to the intestinal lumen is a hallmark of non-typhoidal Salmonella enterica infections in humans. Here, we analyzed the interaction of gut luminal neutrophils with S. enterica serovar Typhimurium (S. Tm) in a mouse colitis model.Upon S. Tm(wt) infection, neutrophils transmigrate across the mucosa into the intestinal lumen. We detected a majority of pathogens associated with luminal neutrophils 20 hours after infection. Neutrophils are viable and actively engulf S. Tm, as demonstrated by live microscopy. Using S. Tm mutant strains defective in tissue invasion we show that pathogens are mostly taken up in the gut lumen at the epithelial barrier by luminal neutrophils. In these luminal neutrophils, S. Tm induces expression of genes typically required for its intracellular lifestyle such as siderophore production iroBCDE and the Salmonella pathogenicity island 2 encoded type three secretion system (TTSS-2). This shows that S. Tm at least transiently survives and responds to engulfment by gut luminal neutrophils. Gentamicin protection experiments suggest that the life-span of luminal neutrophils is limited and that S. Tm is subsequently released into the gut lumen. This "fast cycling" through the intracellular compartment of gut luminal neutrophils would explain the high fraction of TTSS-2 and iroBCDE expressing intra- and extracellular bacteria in the lumen of the infected gut. In conclusion, live neutrophils recruited during acute S. Tm colitis engulf pathogens in the gut lumen and may thus actively engage in shaping the environment of pathogens and commensals in the inflamed gut