Three years of L-band brightness temperature measurements in a mountainous area : topography, vegetation and snowmelt issues

L-band passive measurements (1.4 GHz) over continental areas are known to be related to surface soil moisture. Two satellite missions were recently launched to measure land surface emissions at this frequency band (SMOS-Soil Moisture and Ocean Salinity in 2009 and SMAP-Soil Moisture Active/Passive in 2015). In order to improve soil moisture retrievals from satellite data, ground-based radiometer systems operating at the same frequency were deployed over specified areas to investigate the L-band emission of various land covers under various climatological conditions. In this study, three years of L-band passive measurements from a radiometer installed on top of a steep mountain in the French Alps were analyzed and compared to L-band passive simulations. The innovative radiometer location led to large footprints due to the distance between the radiometer and the area under study. This experiment also produced microwave measurements affected by various potential difficulties typically encountered in SMOS/SMAP satellite missions: topography, heterogeneous footprints, dry/wet snow events, dew and vegetation litter. Based on in situ and modeling data, this paper investigates the potential of a radiative transfer model (L-band Microwave Emission of the Biosphere, L-MEB) to simulate L-band measurements and analyzes the differences with ELBARA observations. First, it was found that the topography generated a mixing of the horizontal and vertical polarizations. In addition, a large positive bias was found on ELBARA measurements (31 K and 12 K in horizontal and vertical polarizations respectively). Investigations showed that the sky reflection measured by the radiometer was partially substituted by land reflection coming from the surrounding topography. Second, the low-vegetation emission was investigated and highlighted the inability of the MODIS NDVI product to correctly represent the vegetation dynamics. Finally, dry snow conditions were found to have non-negligible impact at L-band and a particular signature was found during snow melting periods, with potential applications at the SMOS/SMAP spatial scales (similar to 40 km)

Identification and relatedness of coronatine-producing Pseudomonas syringae pathovars by PCR analysis and sequence determination of the amplification products.

Production of the chlorosis-inducing phytotoxin coronatine in the Pseudomonas syringae pathovars atropurpurea, glycinea, maculicola, morsprunorum, and tomato has been previously reported. DNA hybridization studies previously indicated that the coronatine biosynthetic gene cluster is highly conserved among P. syringae strains which produce the toxin. In the present study, two 17-bp oligonucleotide primers derived from the coronatine biosynthetic gene cluster of P. syringae pv. glycinea PG4180 were investigated for their ability to detect coronatine-producing P. syringae strains by PCR analysis. The primer set amplified diagnostic 0.65-kb PCR products from genomic DNAs of five different coronatine-producing pathovars of P. syringae. The 0.65-kb products were not detected when PCR experiments utilized nucleic acids of nonproducers of coronatine or those of bacteria not previously investigated for coronatine production. When the 0.65-kb PCR products were digested with ClaI, PstI, and SmaI, fragments of identical size were obtained for the five different pathovars of P. syringae. A restriction fragment length polymorphism was detected in the amplified region of P. syringae pv. atropurpurea, since this pathovar lacked a conserved PvuI site which was detected in the PCR products of the other four pathovars. The 0.65-kb PCR products from six strains comprising five different pathovars of P. syringae were cloned and sequenced. The PCR products from two different P. syringae pv. glycinea strains contained identical DNA sequences, and these showed relatedness to the sequence obtained for the pathovar morsprunorum. The PCR products obtained from the pathovars maculicola and tomato were the most similar to each other, which supports the hypothesis that these two pathovars are closely related.(ABSTRACT TRUNCATED AT 250 WORDS

Potencial herbicida da biomassa e de substâncias químicas produzidas pelo fungo endofítico Pestalotiopsis guepinii Herbicide potential of the biomass and chemical compounds produced by the fungus Pestalotiopsis guepinii

Muitas substâncias químicas disponíveis na natureza, produzidas por plantas ou por microrganismos, podem oferecer novas e excelentes oportunidades para diversificar o controle de pragas na agricultura e na prática agrícola, e, nesse sentido, os fungos podem contribuir de forma positiva. O objetivo deste trabalho foi caracterizar o potencial inibitório na germinação de sementes e no desenvolvimento de plântulas de duas espécies de plantas daninhas em relação aos extratos e substâncias químicas obtidas da biomassa produzida por Pestalotiopsis guepinii - um fungo endofítico da espécie Virola michelii. Foram desenvolvidos bioensaios em condições controladas de 25 ºC e fotoperíodo de 12 horas, para germinação, e de 25 ºC e fotoperíodo de 24 horas, para desenvolvimento da radícula e do hipocótilo. Os extratos brutos foram analisados em concentração de 1,0% (m/v). Os resultados indicaram os extratos mais polares (MeOH-1 e MeOH-2) como de maior potencial inibitório, porém os efeitos promovidos pelos extratos hexânicos e acetato de etila foram expressivos, especialmente em relação à germinação das sementes. Comparativamente, a germinação das sementes das espécies de plantas daninhas se mostrou mais sensível aos efeitos do que o desenvolvimento das plântulas. Das espécies receptoras, Mimosa pudica (malícia) apresentou maior sensibilidade aos efeitos inibitórios dos extratos. Entretanto, na germinação de sementes da espécie Senna obtusifolia (mata-pasto), o extrato MeOH-1 apresentou 100% de inibição. As substâncias ergosterol e peróxido de ergosterol, isoladas do extrato hexânico, quando testadas isoladamente, apresentaram potencial inibitório sempre abaixo dos 35%, não repetindo o potencial inibitório do extrato hexânico, de onde foram isoladas. Quando testadas juntas, não se verificaram aumentos expressivos na atividade herbicida, embora acréscimos na atividade inibitória tenham sido observados.<br>Many of the chemical compounds found in nature that are produced by plants or microorganisms can offer new and efficient ways of controlling pests in agriculture and agricultural practice, with the help of fungi. The objective of this work is to characterize the inhibitory potential for seed germination and the plantlet development of two weed species using extracts and compounds obtained from biomass produced by Pestalotiopsis guepinii, an endophytic fungus of the species Virola michelii. The bioassays were developed under controlled conditions at 25 ºC and 12-hour photoperiod for germination, and at 25 ºC and 12-hour photoperiod for root and hypocotyl development. The crude extracts were analyzed at a concentration of 1.0% (m/v). The results showed that the more polar extracts (MeOH-1 and MeOH-2) have the highest inhibitory potential, although the hexane and ethyl acetate extract effects were important, especially for seed germination. Comparatively, weed seed germination was more sensitive to the effects than plantlet development. Mimosa pudica was more affected by the inhibitory effects of the extracts. However, for seed germination of Senna obtusifolia, the extract MeOH-1 showed 100% inhibition. The compounds ergosterol and ergosterol peroxide showed an inhibitory potential always below 35%, not showing the inhibitory potential of the hexane extract from which they were isolated. When these compounds were tested together, little increase was observed in the inhibitory activity

Soil Hydraulic Parameters of Bare Soil Plots with Different Soil Structure Inversely Derived from L-Band Brightness Temperatures

The soil structure and the hydraulic properties of the top soil layer control water exchanges between the soil and the atmosphere. We used L-band radiometry to infer the hydraulic properties of the top layer of three field plots with different soil structure.The structure of the surface layer of the soil is strongly influenced by soil tillage practices, with important consequences for the hydraulic properties and soil moisture dynamics in the top soil layer. In this study, during four 28-d periods, we monitored L-band brightness temperatures and infrared (IR) temperatures over bare silt loam soil plots with different soil surface structure: tilled, seedbed, and compacted plots. Differences in absolute and normalized L-band brightness temperatures between the plots indicated that plot specific roughness, soil moisture contents, and soil hydraulic properties might be inverted from L-band brightness temperatures using a coupled radiative transfer, roughness correction, and soil hydrological model. The inversely estimated surface roughness parameters compared well with those derived from laser profiler measurements. The estimated saturated water contents of the tilled and seedbed plots were larger than the one of the compacted plot, and the unsaturated hydraulic conductivity was smaller in the former plots than in the compacted plot for more negative pressure heads. These differences in hydraulic properties translated into larger dynamics of the simulated soil moisture during a 28-d measurement period in the tilled and seedbed plots than in the compacted plot. This difference could be confirmed qualitatively but not quantitatively by in situ soil moisture measurements. Furthermore, differences in simulated actual evaporation rates between the plots were confirmed by observed differences in measured IR temperatures. The results indicate that effects of soil management on soil surface roughness and soil hydraulic properties could be inferred from L-band brightness temperatures