Collection protocols for the recovery of biological samples

The main focus in forensic genetics for two decades has been to improve the extraction of DNA from a wide variety of evidence and to make the profiling technology more sensitive and robust. In contrast, the recovery methods for biological material have seen little development. This study aims to improve the efficacy of the collection and storage processes, from crime scene to receipt at the laboratory. This study compared the use of ultrapure water as a wetting agent when collecting biological evidence using swabs with a detergent-based buffer. The results show that the stability post-collection greatly improved by using a newly developed buffer. When ultrapure water is used, DNA degradation was seen after 6 h at room temperature. However, the detergent-based buffer stabilized DNA for up to 48 h, even when the temperature was increased to 50 °C. The impact of these findings may be limited where crime scene evidence can be refrigerated until it reaches the laboratory. However, there are many situations/contexts where sample refrigeration is not possible and there is scope to improve the preservation of the genetic forensic evidence before it reaches the laboratory

Engineering workstation: Sensor modeling

The purpose of the engineering workstation is to provide an environment for rapid prototyping and evaluation of fusion and image processing algorithms. Ideally, the algorithms are designed to optimize the extraction of information that is useful to a pilot for all phases of flight operations. Successful design of effective fusion algorithms depends on the ability to characterize both the information available from the sensors and the information useful to a pilot. The workstation is comprised of subsystems for simulation of sensor-generated images, image processing, image enhancement, and fusion algorithms. As such, the workstation can be used to implement and evaluate both short-term solutions and long-term solutions. The short-term solutions are being developed to enhance a pilot's situational awareness by providing information in addition to his direct vision. The long term solutions are aimed at the development of complete synthetic vision systems. One of the important functions of the engineering workstation is to simulate the images that would be generated by the sensors. The simulation system is designed to use the graphics modeling and rendering capabilities of various workstations manufactured by Silicon Graphics Inc. The workstation simulates various aspects of the sensor-generated images arising from phenomenology of the sensors. In addition, the workstation can be used to simulate a variety of impairments due to mechanical limitations of the sensor placement and due to the motion of the airplane. Although the simulation is currently not performed in real-time, sequences of individual frames can be processed, stored, and recorded in a video format. In that way, it is possible to examine the appearance of different dynamic sensor-generated and fused images

Particle Acceleration in Turbulence and Weakly Stochastic Reconnection

Fast particles are accelerated in astrophysical environments by a variety of processes. Acceleration in reconnection sites has attracted the attention of researchers recently. In this letter we analyze the energy distribution evolution of test particles injected in three dimensional (3D) magnetohydrodynamic (MHD) simulations of different magnetic reconnection configurations. When considering a single Sweet-Parker topology, the particles accelerate predominantly through a first-order Fermi process, as predicted in previous work (de Gouveia Dal Pino & Lazarian, 2005) and demonstrated numerically in Kowal, de Gouveia Dal Pino & Lazarian (2011). When turbulence is included within the current sheet, the acceleration rate, which depends on the reconnection rate, is highly enhanced. This is because reconnection in the presence of turbulence becomes fast and independent of resistivity (Lazarian & Vishniac, 1999; Kowal et al., 2009) and allows the formation of a thick volume filled with multiple simultaneously reconnecting magnetic fluxes. Charged particles trapped within this volume suffer several head-on scatterings with the contracting magnetic fluctuations, which significantly increase the acceleration rate and results in a first-order Fermi process. For comparison, we also tested acceleration in MHD turbulence, where particles suffer collisions with approaching and receding magnetic irregularities, resulting in a reduced acceleration rate. We argue that the dominant acceleration mechanism approaches a second order Fermi process in this case.Comment: 6 pages, 1 figur

Biosafety of bee pollinators in genetically modified agro-ecosystems : current approach and further development in the EU

Bee pollinators are an important guild delivering a fundamental input to European agriculture due to the ecological service they provide to crops in addition to the direct economic revenues from apiculture. Bee populations are declining in Europe as a result of the effects of several environmental stressors, both natural and of anthropic origin. Efforts are ongoing in the European Union (EU) to improve monitoring and management of pollinator populations to arrest further declines. Genetically modified (GM) crops are currently cultivated in a limited area in Europe, and an environmental risk assessment (ERA) is required prior to their authorization for cultivation. The possible impacts of GM crops on pollinators are deemed relevant for the ERA. Existing ecotoxicological studies indicate that traits currently expressed in insect-resistant GM plants are unlikely to represent a risk for pollinators. However, new mechanisms of insect resistance are being introduced into GM plants, including novel combinations of Cry toxins and double strand RNA (dsRNA), and an ERA is required to consider lethal and sublethal effects of these new products on nontarget species, including insect pollinators. The evaluation of indirect effects linked to the changes in management practices (e.g. for herbicide-tolerant GM crops) is an important component of EU regulations and a requirement for ERA. This paper reviews current approaches used to test the sensitivity of pollinators to GM plants and their products to determine whether sufficient data are being provided on novel GM plants to satisfy EU risk assessment requirements

Scanning a photonic crystal slab nanocavity by condensation of xenon

Allowing xenon or nitrogen gas to condense onto a photonic crystal slab nanocavity maintained at 10–20 K results in shifts of the nanocavity mode wavelength by as much as 5 nm (~=4 meV). This occurs in spite of the fact that the mode defect is achieved by omitting three holes to form the spacer. This technique should be useful in changing the detuning between a single quantum dot transition and the nanocavity mode for cavity quantum electrodynamics experiments, such as mapping out a strong coupling anticrossing curve. Compared with temperature scanning, it has a much larger scan range and avoids phonon broadening

Bibliography on heavy vehicle dynamics

http://deepblue.lib.umich.edu/bitstream/2027.42/108243/1/103019.pdfDescription of 103019.pdf : Bibliograph

Magnetic Reconnection with Radiative Cooling. I. Optically-Thin Regime

Magnetic reconnection, a fundamental plasma process associated with a rapid dissipation of magnetic energy, is believed to power many disruptive phenomena in laboratory plasma devices, the Earth magnetosphere, and the solar corona. Traditional reconnection research, geared towards these rather tenuous environments, has justifiably ignored the effects of radiation on the reconnection process. However, in many reconnecting systems in high-energy astrophysics (e.g., accretion-disk coronae, relativistic jets, magnetar flares) and, potentially, in powerful laser plasma and z-pinch experiments, the energy density is so high that radiation, in particular radiative cooling, may start to play an important role. This observation motivates the development of a theory of high-energy-density radiative magnetic reconnection. As a first step towards this goal, we present in this paper a simple Sweet--Parker-like theory of non-relativistic resistive-MHD reconnection with strong radiative cooling. First, we show how, in the absence of a guide magnetic field, intense cooling leads to a strong compression of the plasma in the reconnection layer, resulting in a higher reconnection rate. The compression ratio and the layer temperature are determined by the balance between ohmic heating and radiative cooling. The lower temperature in the radiatively-cooled layer leads to a higher Spitzer resistivity and hence to an extra enhancement of the reconnection rate. We then apply our general theory to several specific astrophysically important radiative processes (bremsstrahlung, cyclotron, and inverse-Compton) in the optically thin regime, for both the zero- and strong-guide-field cases. We derive specific expressions for key reconnection parameters, including the reconnection rate. We also discuss the limitations and conditions for applicability of our theory.Comment: 31 pages, 1 figur