355 research outputs found
Rapid Initial Morphospace Expansion and Delayed Morphological Disparity Peak in the First 100 Million Years of the Archosauromorph Evolutionary Radiation
Adaptive radiations have played a major role in generating modern and deep-time biodiversity. The Triassic radiation of the Archosauromorpha was one of the most spectacular vertebrate radiations, giving rise to many highly ecomorphologically varied lineagesâincluding the dinosaurs, pterosaurs, and stem-crocodyliansâthat dominated the larger-bodied land fauna for the following 150 Ma, and ultimately gave rise to todayâs > 10,000 species of birds and crocodylians. This radiation provides an outstanding testbed for hypotheses relating to adaptive radiations more broadly. Recent studies have started to characterize the tempo and mode of the archosauromorph early adaptive radiation, indicating very high initial rates of evolution, non-competitive niche-filling processes, and previously unrecognized morphological disparity even among non-crown taxa. However, these analyses rested primarily either on discrete characters or on geometric morphometrics of the cranium only, or even failed to fully include phylogenetic information. Here we expand previous 2D geometric morphometric cranial datasets to include new taxa and reconstructions, and create an analogous dataset of the pelvis, thereby allowing comparison of anatomical regions and the transition from âsprawlingâ to âuprightâ posture to be examined. We estimated morphological disparity and evolutionary rates through time. All sampled clades showed a delayed disparity peak for sum of variances and average nearest neighbor distances in both the cranium and pelvis, with disparity likely not saturated by the end of the studied time span (Late Jurassic); this contrasts with smaller radiations, but lends weight to similar results for large, ecomorphologically-varied groups. We find lower variations in pelvic than cranial disparity among Triassic-Jurassic archosaurs, which may be related to greater morphofunctional constraints on the pelvis. Contrasting with some previous work, but also confirming some previous findings during adaptive radiations, we find relatively widespread evidence of correlation between sampled diversity and disparity, especially at the largest phylogenetic scales and using average displacement rather than sum of variances as disparity metric; this also demonstrates the importance of comparing disparity metrics, and the importance of phylogenetic scale. Stem and crown archosauromorphs show a morphological diversification of both the cranium and pelvis with higher initial rates (PermianâMiddle Triassic and at the base of major clades) followed by lower rates once diversification into niches has occurred (Late TriassicâJurassic), indicating an âearly burstâ pattern sensu lato. Our results provide a more detailed and comprehensive picture of the early archosauromorph radiation and have significant bearing on the understanding of deep-time adaptive radiations more broadly, indicating widespread patterns of delayed disparity peaks, initial correlation of diversity and disparity, and evolutionary early bursts.Fil: Foth, Christian. University of Fribourg; Alemania. Staatliches Museum fur Naturkunde Stuttgart; AlemaniaFil: Sookias, Roland B.. UniversitĂŠ de Liège; BĂŠlgica. University of Oxford; Reino Unido. Staatliches Museum fur Naturkunde Stuttgart; AlemaniaFil: Ezcurra, Martin Daniel. Consejo Nacional de Investigaciones CientĂficas y TĂŠcnicas. Oficina de CoordinaciĂłn Administrativa Parque Centenario. Museo Argentino de Ciencias Naturales "Bernardino Rivadavia"; Argentin
Optimal estimation of water vapour profiles using a combination of Raman lidar and microwave radiometer
In this work, a two-step algorithm to obtain water vapour profiles from
a combination of Raman lidar and microwave radiometer is presented. Both
instruments were applied during an intensive 2-month measurement campaign
(HOPE) close to JĂźlich, western Germany, during spring 2013. To retrieve
reliable water vapour information from inside or above the cloud a two-step
algorithm is applied. The first step is a Kalman filter that extends the
profiles, truncated at cloud base, to the full height range (up to 10âŻkm) by
combining previous information and current measurement. Then the complete
water vapour profile serves as input to the one-dimensional variational
(1D-VAR) method, also known as optimal estimation. AÂ forward model simulates
the brightness temperatures which would be observed by the microwave
radiometer for the given atmospheric state. The profile is iteratively
modified according to its error bars until the modelled and the actually
measured brightness temperatures sufficiently agree. The functionality of the
retrieval is presented in detail by means of case studies under different
conditions. AÂ statistical analysis shows that the availability of Raman lidar
data (night) improves the accuracy of the profiles even under cloudy
conditions. During the day, the absence of lidar data results in larger
differences in comparison to reference radiosondes. The data availability of
the full-height water vapour lidar profiles of 17âŻ% during the 2-month
campaign is significantly enhanced to 60âŻ% by applying the retrieval. The
bias with respect to radiosonde and the retrieved a posteriori uncertainty of
the retrieved profiles clearly show that the application of the Kalman filter
considerably improves the accuracy and quality of the retrieved mixing ratio
profiles
Utilizing Multimedia Case Studies to Teach the Professional Side of Project Management
This research was conducted to evaluate the effectiveness of using a LITEE multimedia case study to teaching concepts in engineering courses. The LITEE Superstar case study was implemented in an engineering Project Management course. Numerous surveys regarding student expectations, outcomes, and attitudes were collected and results are presented herein. Overall, the study provided evidence that the students felt that the LITEE case study added value to the course via the different methods of teaching material, aided in the understanding of the project selection process, and ultimately helped them be successful in their course project which was conducted for a real rural Missouri city. The data was unable to be tracked on a per student basis; this yields areas for future research to track individual student improvement and attitudes. This research provides evidence that using multimedia case studies, such as the case studies published by LITEE, are effective and well received by engineering students in their coursework
Nonlinearity of Mechanochemical Motions in Motor Proteins
The assumption of linear response of protein molecules to thermal noise or
structural perturbations, such as ligand binding or detachment, is broadly used
in the studies of protein dynamics. Conformational motions in proteins are
traditionally analyzed in terms of normal modes and experimental data on
thermal fluctuations in such macromolecules is also usually interpreted in
terms of the excitation of normal modes. We have chosen two important protein
motors - myosin V and kinesin KIF1A - and performed numerical investigations of
their conformational relaxation properties within the coarse-grained elastic
network approximation. We have found that the linearity assumption is deficient
for ligand-induced conformational motions and can even be violated for
characteristic thermal fluctuations. The deficiency is particularly pronounced
in KIF1A where the normal mode description fails completely in describing
functional mechanochemical motions. These results indicate that important
assumptions of the theory of protein dynamics may need to be reconsidered.
Neither a single normal mode, nor a superposition of such modes yield an
approximation of strongly nonlinear dynamics.Comment: 10 pages, 6 figure
Speciation of phosphorus in a fertilized, reduced-till soil system: in-field treatment incubation study
Citation: Khatiwada, Raju, Ganga M. Hettiarachchi, David B. Mengel, and Mingwei Fei. âSpeciation of Phosphorus in a Fertilized, Reduced-Till Soil System: In-Field Treatment Incubation Study.â Soil Science Society of America Journal 76, no. 6 (2012): 2006â18. https://doi.org/10.2136/sssaj2011.0299.Phosphorus management in reduced-tillage systems is a great concern for farmers. Conclusive positive results of deep-banding P fertilizers compared with broadcast application and the chemistry of reduced-tillage systems remain unclear. Knowledge of the dominant solid P species present in soil following application of P fertilizers and the resulting potential P availability would help us understand and efficiently manage P in reduced-tillage systems. The objective of this research was to study the influence of placement (broadcast vs. deep-band P), fertilizer source (granular vs. liquid P), and time on the reaction products of P under field conditions. Changes in soil pH, resin-extractable P, total P, and speciation of P were determined at different distances from the point of fertilizer application at 5 wk and 6 mo after P application at a rate of 75 kg haâ1 to a soil system that was under long-term reduced tillage. Resin-extractable P was lower for broadcast treatments compared with deep-band treatments for both time periods. Resin-extractable P was greater in the liquid P-treated soils than in the granular P-treated soils. Speciation results showed that granular P fertilizers tended to form FeâP-like forms, whereas liquid forms remained in adsorbed P-like forms in the soil 5 wk after application; moreover, speciation results showed granular P fertilizers precipitated less when deep-banded. During the 6-mo period following application, reaction products of broadcast granular, broadcast liquid, and deep-band granular fertilizers transformed to Ca-phosphate or mixtures of Ca-, Fe- and adsorbed-phosphate-like forms, whereas deep-band liquid P remained as mainly adsorbed P-like forms. Deep-banding of P would most likely provide a solution that is both agronomically and environmentally efficient for reduced-till farmers
The function of the alula in avian flight
The alula is a small structure located at the joint between the hand-wing and arm-wing of birds and is known to be used in slow flight with high angles of attack such as landing. It is assumed to function similarly to a leading-edge slat that increases lift and delays stall. However, in spite of its universal presence in flying birds and the wide acceptance of stall delay as its main function, how the alula delays the stall and aids the flight of birds remains unclear. Here, we investigated the function of alula on the aerodynamic performance of avian wings based on data from flight tasks and wind-tunnel experiments. With the alula, the birds performed steeper descending flights with greater changes in body orientation. Force measurements revealed that the alula increases the lift and often delays the stall. Digital particle image velocimetry showed that these effects are caused by the streamwise vortex, formed at the tip of the alula, that induces strong downwash and suppresses the flow separation over the wing surface. This is the first experimental evidence that the alula functions as a vortex generator that increases the lift force and enhances manoeuvrability in flights at high angles of attack.open1
Biogenesis of the inner membrane complex is dependent on vesicular transport by the alveolate specific GTPase Rab11B
Apicomplexan parasites belong to a recently recognised group of protozoa referred to as Alveolata. These protists contain membranous sacs (alveoli) beneath the plasma membrane, termed the Inner Membrane Complex (IMC) in the case of Apicomplexa. During parasite replication the IMC is formed de novo within the mother cell in a process described as internal budding. We hypothesized that an alveolate specific factor is involved in the specific transport of vesicles from the Golgi to the IMC and identified the small GTPase Rab11B as an alveolate specific Rab-GTPase that localises to the growing end of the IMC during replication of Toxoplasma gondii. Conditional interference with Rab11B function leads to a profound defect in IMC biogenesis, indicating that Rab11B is required for the transport of Golgi derived vesicles to the nascent IMC of the daughter cell. Curiously, a block in IMC biogenesis did not affect formation of sub-pellicular microtubules, indicating that IMC biogenesis and formation of sub-pellicular microtubules is not mechanistically linked. We propose a model where Rab11B specifically transports vesicles derived from the Golgi to the immature IMC of the growing daughter parasites
Phosphoenolpyruvate carboxylase dentified as a key enzyme in erythrocytic Plasmodium falciparum carbon metabolism
Phospoenolpyruvate carboxylase (PEPC) is absent from humans but encoded in thePlasmodium falciparum genome, suggesting that PEPC has a parasite-specific function. To investigate its importance in P. falciparum, we generated a pepc null mutant (D10Îpepc), which was only achievable when malate, a reduction product of oxaloacetate, was added to the growth medium. D10Îpepc had a severe growth defect in vitro, which was partially reversed by addition of malate or fumarate, suggesting that pepc may be essential in vivo. Targeted metabolomics using 13C-U-D-glucose and 13C-bicarbonate showed that the conversion of glycolytically-derived PEP into malate, fumarate, aspartate and citrate was abolished in D10Îpepc and that pentose phosphate pathway metabolites and glycerol 3-phosphate were present at increased levels. In contrast, metabolism of the carbon skeleton of 13C,15N-U-glutamine was similar in both parasite lines, although the flux was lower in D10Îpepc; it also confirmed the operation of a complete forward TCA cycle in the wild type parasite. Overall, these data confirm the CO2 fixing activity of PEPC and suggest that it provides metabolites essential for TCA cycle anaplerosis and the maintenance of cytosolic and mitochondrial redox balance. Moreover, these findings imply that PEPC may be an exploitable target for future drug discovery
Calmodulin-like proteins localized to the conoid regulate motility and cell invasion by Toxoplasma gondii
Toxoplasma gondii contains an expanded number of calmodulin (CaM)-like proteins whose functions are poorly understood. Using a combination of CRISPR/Cas9-mediated gene editing and a plant-like auxin-induced degron (AID) system, we examined the roles of three apically localized CaMs. CaM1 and CaM2 were individually dispensable, but loss of both resulted in a synthetic lethal phenotype. CaM3 was refractory to deletion, suggesting it is essential. Consistent with this prediction auxin-induced degradation of CaM3 blocked growth. Phenotypic analysis revealed that all three CaMs contribute to parasite motility, invasion, and egress from host cells, and that they act downstream of microneme and rhoptry secretion. Super-resolution microscopy localized all three CaMs to the conoid where they overlap with myosin H (MyoH), a motor protein that is required for invasion. Biotinylation using BirA fusions with the CaMs labeled a number of apical proteins including MyoH and its light chain MLC7, suggesting they may interact. Consistent with this hypothesis, disruption of MyoH led to degradation of CaM3, or redistribution of CaM1 and CaM2. Collectively, our findings suggest these CaMs may interact with MyoH to control motility and cell invasion
The DACAPO-PESO campaign: Dynamics, Aerosol, Cloud and Precipitation Observations in the Pristine Environment of the Southern Ocean: An overview
This article gives an overview of the DACAPO-PESO field experiment,
which has taken place in Punta Arenas, Chile, from November 2018 to November 2021,
and showcases first exciting research results that have already emerged from it.In diesem Artikel wird ein Ăberblick Ăźber das DACAPO-PESO
Experiment gegeben, welches von November 2018 bis November 2021 in Punta Arenas,
Chile, stattgefunden hat. AuĂerdem werden erste spannende Forschungsergebnisse
vorgestellt, die bereits daraus gewonnen wurden
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