Accumulation of copy-back viral genomes during respiratory syncytial virus infection is preceded by diversification of the copy-back viral genome population followed by selection

RNA viruses generate nonstandard viral genomes during their replication, including viral genomes of the copy-back (cbVGs) type that cannot replicate in the absence of a standard virus. cbVGs play a crucial role in shaping virus infection outcomes due to their ability to interfere with virus replication and induce strong immune responses. However, despite their critical role during infection, the principles that drive the selection and evolution of cbVGs within a virus population are poorly understood. As cbVGs are dependent on the virus replication machinery to be generated and replicated, we hypothesized that host factors that affect virus replication exert selective pressure on cbVGs and drive their evolution within a virus population. To test this hypothesis, we used respiratory syncytial virus (RSV) as a model and took an experimental evolution approach by serially passaging RSV in immune-competent human lung adenocarcinoma A549 control and immune-deficient A549 Signal transducer and activator of transcription 1 (STAT1) KO cells, which allow higher levels of virus replication. As predicted, we observed that virus populations accumulated higher amounts of cbVGs in the more permissive A549 STAT1 KO cells over time; however, unexpectedly, the predominant cbVG species after passages in the two conditions were different. While A549 STAT1 KO cells accumulated relatively short cbVGs, A549 control cells mainly contained cbVGs of much longer predicted size, which have not been described previously. These long cbVGs were predominant at first in both cell line

Effective analysis of secure web response time

The measurement of response time in web based applications is a common task for the evaluation of service responsiveness and the detection of network or server problems. Traffic analysis is the most common strategy for obtaining response time measurements. However, when the traffic is encrypted, the analysis tools cannot provide these measurement results. In this paper we propose a methodology for measuring the response time in HTTPS traffic based on the flow of data in each direction. We have validated the tool with real traffic and with a worst case scenario created in a testbed. When pipelining is present in the encrypted HTTP 1.1 traffic, it results in a small error in the measurement (between 5% and 15% of error for the 99.9 percentile of the real response time). However, pipelining support has almost disappeared from modern web browsers; this makes the estimation provided by this methodology very accurate in real traffic measurements, even for low probability response times. More than 98.8% of the over 8.6 million request-response times we measured in our campus Internet link were obtained without any error.This work was supported by Spanish MINECO through project PIT (TEC2015-69417-C2-2-R)

PELFI Project: Recruitment and Sociodemographic Characteristics of Immigrant and Autochthonous Families from Alicante and Barcelona City Subcohorts

Este artículo corresponde al “Proyecto de Estudios Longitudinales de Familias Inmigradas (PELFI)” del Subprograma de Inmigración y Salud del CIBERESP y describe el trabajo de campo basal y principales características socio-demográficas de dos sub-cohortes de familias inmigrantes y autóctonas. El diseño es observacional prospectivo. La población de estudio se definió como una muestra no probabilística de 180 familias de origen colombiano, ecuatoriano y marroquí y 50 españolas. Se entrevistó a 473 personas adultas entre 18 y 65 años (59,8% mujeres, 68,5% ocupados/as) y a 304 adolescentes entre 12 y17 años (53,9% mujeres, 27,1% nacidos en España pero de padres inmigrados) de cada familia, mediante dos cuestionarios diseñados ad hoc. La tasa de cooperación fue del 82,0% con una velocidad media de reclutamiento de 1,3 familias diarias. En total, se reclutó a 250 familias, 82 procedentes de Ecuador, 82 de Colombia, 29 de Marruecos y 57 españolas. Los adultos inmigrados llevaban una media de 13 años en España. La combinación de técnicas no probabilísticas permitió el acceso y velocidad de reclutamiento. Este estudio aporta información clave para el diseño y mejora de este tipo de cohortes en familias inmigradas.This study is a part of the multi-centre project “Platform of Longitudinal Studies of Immigrant Families (PELFI)” of the Immigration and Health Subprogram of the CIBER-ESP. It describes the field work and data collection of two sub-cohorts of immigrant and native families, and their main socio-demographic characteristics. Prospective observational cohort study in carried out in Barcelona and Alicante, Spain. The study population is a non-probabilistic sample of 180 families of Colombian, Ecuadorian and Moroccan origin and 50 families of Spanish origin. We interviewed adults aged 18-65 years and adolescents aged 12-17 years in each family, through two questionnaires (adolescent/adult). The cooperation rate was 82.0% with an average recruitment rate of 1.3 families per day. In total, 250 families have been recruited, 82 from Ecuador, 82 from Colombia, 29 from Morocco and 57 from Spain. A total of 473 adults (59.8% women and 68.5% employed) were surveyed. Immigrant adults have an average of 13 years living in Spain. A total of 304 adolescents (53.9% female, 27.1% born in Spain but with immigrant parents) were surveyed. The combination of non-probabilistic techniques promoted access and improved recruitment speed. This study provides key information for the design and improvement of cohort studies with immigrant families.Proyectos Fondo Investigación Sanitaria números PI14/01146 y PI14/02005 e Instituto de Salud Carlos III-FEDER

Encounters and places: Project negotiations in Galessa, Ethiopia

Purpose – Reflections on negotiation processes between farmers and scientists in research projects provide insights into issues of participation, power and equity. The purpose of this paper is to illustrate how actors chose places to meet, negotiate and represent technologies. Design/methodology/approach – The research involved semi-structured interviews and participant observation with farmers, scientists, government agricultural advisors (extensionists), policymakers, and staff of development organisations involved in a research for development project in Western Shewa, Ethiopia from 2009 to 2011. It combines theories from social studies of science as well as development studies. Findings – Using blueprint approaches in research projects will not yield sustainable results. Participation must go beyond consultation or trying to educate farmers. Social relations are at the core of cooperation between farmers and scientists and require much more attention. Powerful choices on modes of representation and communication technologies as well as unilateral decisions on places have important implications for the way decisions are eventually made and by whom. Originality/value – This approach to studying research for development projects from social studies of science perspectives adds new insights into debates on participation and power in technology transfer and multicultural cooperation in rural development

Plutonium removal limit for the disposition of plutonium-bearing materials

Recent changes in world politics have resulted in the United States reducing its nuclear weapons and stopping plutonium production. Prior plutonium production, dismantling warheads, and decontamination and decommissioning some facilities have produced plutonium-bearing materials which must continue to be managed. As each lot of material is processed, the processor must decide whether to remove the plutonium before discarding the material or to discard it without plutonium removal. DOE has developed a new method of making this decision, called the Plutonium Removal Limit System (PRLS). The system is based on defining a plutonium concentration above which the cost of disposing of plutonium-bearing materials will be less if plutonium is recovered and below which the cost will be less if plutonium is discarded (following suitable waste treatment). This method minimizes the overall cost to DOE for disposing of the existing inventory of plutonium-bearing materials. The method was used to analyze the plutonium-discard limit for all categories of plutonium-bearing materials currently at each site. This analysis indicated the need to standardize the way sites make the remove-versus-discard decision. For this purpose, a set of departmental plutonium removal limits was developed. DOE expects to approve implementing this new method at all facilities handling plutonium-bearing material in FY 93

Academic freedom in Europe: time for a Magna Charta?

This paper is a preliminary attempt to establish a working definition of academic freedom for the European Union states. The paper details why such a definition is required for the European Union and then examines some of the difficulties of defining academic freedom. By drawing upon experience of the legal difficulties beset by the concept in the USA and building on previous analyses of constitutional and legislative protection for academic freedom, and of legal regulations concerning institutional governance and academic tenure, a working definition of academic freedom is then derived. The resultant definition which, it is suggested, could form the basis for a European Magna Charta Libertatis Academicae, goes beyond traditional discussions of academic freedom by specifying not only the rights inherent in the concept but also its accompanying duties, necessary limitations and safeguards. The paper concludes with proposals for how the definition might be tested and carried forward

A social licence for science: capturing the public or co-constructing research?

The “social licence to operate” has been invoked in science policy discussions including the 2007 Universal Ethical Code for scientists issued by the UK Government Office for Science. Drawing from sociological research on social licence and STS interventions in science policy, the authors explore the relevance of expectations of a social licence for scientific research and scientific contributions to public decision-making, and what might be involved in seeking to create one. The process of seeking a social licence is not the same as trying to create public or community acceptance for a project whose boundaries and aims have already been fully defined prior to engagement. Such attempts to “capture” the public might be successful from time to time but their legitimacy is open to question especially where their engagement with alternative research futures is “thin”. Contrasting a national dialogue on stem cells with the early history of research into bioenergy, we argue that social licence activities need to be open to a “thicker” engagement with the social. Co-constructing a licence suggests a reciprocal relationship between the social and the scientific with obligations for public and private institutions that shape and are shaped by science, rather than just science alone

Preventing and addressing intimate partner violence against migrant and ethnic minority women: the role of the health sector

Violence against women is an extreme manifestation of gender inequality in society and a serious violation of fundamental human rights. Intimate partner violence (IPV) is the most common type of such violence and takes place within couples. IPV can lead to death, physical injury, functional impairment, mental health problems, negative health behaviour, chronic conditions and reproductive health problems. Institutional discrimination, lack of access to or knowledge of services, and cultural differences can prevent women who are not only experiencing IPV but also migrants or members of ethnic minorities from seeking help. This policy brief aims to provide input into the role of the health sector in preventing and addressing IPV among migrant women and those of ethnic minorities. It describes the scope of the problem, presenting key evidence, and makes recommendations for health policy and health systems, health facilities and health service providers

The first bite: Imaginaries, promotional publics and the laboratory grown burger

In this paper we analyse a 2013 press conference hosting the world’s first tasting of a laboratory grown hamburger. We explore this as a media event: an exceptional performative moment in which common meanings are mobilised and a connection to a shared centre of reality is offered. We develop our own theoretical contribution – the promotional public – to characterise the affirmative and partial patchwork of carefully selected actors invoked during the burger tasting. Our account draws upon three areas of analysis: interview data with the scientists who developed the burger, media analysis of the streamed press conference itself, and media analysis of the social media tail during and following the event. We argue that the call to witness an experiment is a form of promotion but that such promotional material also offers an address that invokes a public with its attendant tensions.The research leading to this publication has received funding from the European Community’s Seventh Framework Programme (FP7/2007–2013) under grant agreement number 288971 (EPINET). Neil Stephens’ involvement has also received the support of the Economic and Social Research Council (ESRC). His work is part of the Research Programme of the ESRC Genomics Network at Cesagen (ESRC Centre for Economic and Social Aspects of Genomics). Neil Stephens’ work was also supported by the Wellcome Trust (WT096541MA) and a visiting scholarship to CGS Centre for Society and Genomics in The Netherlands, May to July 2011. This support is gratefully acknowledge

The nuanced nature of work quality : evidence from rural Newfoundland and Ireland

This article explores the relationship between job and work quality and argues that while it is important to examine job quality, to understand workers’ experiences fully, the focus should be on the broader concept of work quality, which places the job against its wider socio-economic context. Based on the experiences of 88 rural workers gathered via interviews in Newfoundland and Ireland, it appears that the same or similar jobs can be regarded very differently depending upon the context in which they are embedded, as people at different locations and/or stages of life have an individual set of aspirations, expectations and life experiences. The study found that the factors that affect work quality are moulded by broader aspects of life – family, friends, community, lifestyle and past experiences – that shape an individual