Pharmacokinetics of 1,25-dihydroxyvitamin D3 glycosides from Solanum glaucophyllum extract given in a rumen bolus on blood mineral profiles in dry pregnant dairy cows.

Providing tablets of 1,25-dihydroxyvitamin D3 (1,25(OH)2D3), the biologically active metabolite of vitamin D3, in a rumen bolus may be used as prevention for periparturient hypocalcemia in dairy cows. This study investigated the pharmacokinetics of 1,25(OH)2D3 glycosides extracted from Solanum glaucophyllum (SGE) on blood serum 1,25(OH)2D3, Ca, P and Mg response in dry pregnant dairy cows. Boluses contained tablets of SGE which differed in their release properties (rapid release, slow release and combination) and galenics (200 μg uncoated, 300 μg and 500 μg uncoated or coated, 2 × 500 μg uncoated). Nineteen blood samples were collected from 29 cows between 96 h before and 336 h after bolus administration. Blood serum 1,25(OH)2D3, Ca and P increased between 12 h and 120 h, 12 h and 264 h and 24 h and 264 h, respectively. Highest values were reached at 30 h, 72 h and 120 h for 1,25(OH)2D3, Ca and P, respectively. Baseline values were then reached at 216 h for 1,25(OH)2D3 and 336 h for Ca and P. Concentration of Mg decreased between 24 h and 216 h, before reaching values comparable to baseline at 264 h. Highest Ca values were obtained with the combined rapid and slow release properties (500 μg) and there was no effect from coating on pharmacokinetics. In conclusion, the antepartum oral SGE bolus administration may be suitable for the prevention of periparturient hypocalcemia

Potential of a rumen bolus containing 1,25-dihydroxyvitamin D3 glycosides for the prevention of hypocalcaemia in primiparous and multiparous dairy cows

Periparturient hypocalcaemia is a widespread metabolic disorder in dairy cows. Clinical and subclinical cases occur primarily in multiparous (Multi) cows, but subclinical cases have also been reported in primiparous (Primi) cows. A preventive strategy was investigated by administering the physiologically active vitamin D3 metabolite, 1,25-dihydroxyvitamin D3 (1,25-dihydroxycholecalciferol, 1,25(OH)2D3) as a rumen bolus. The bolus contained tablets of 1,25(OH)2D3 glycoside extract from Solanum glaucophyllum (SGE), releasing SGE over several days. The aim was to study the effect of a bolus containing 0 (C) or 500 µg (SGE) of 1,25(OH)2D3 on 1,25(OH)2D3 and mineral status in periparturient cows up to three weeks into lactation and on colostrum, milk and calves' blood mineral contents. The bolus was administered three to four days prior to expected calving to Primi and Multi cows fed a herbage-based diet (dietary cation-anion difference of +522 mEq/kg DM). One C or SGE bolus was applied to 12 Primi and 12 Multi cows. Blood was regularly sampled (and selected a posteriori for antepartum samples) in regard to the actual calving day (d0), immediately prior to bolus application and at day -2, 0.5, 1, 1.5, 2, 4, 8, 11, 15, 18 and 22. Additional samples included urine (at bolus application, d0.5 and d2), colostrum, milk samples (weekly) and calves' blood (d2). Blood serum 1,25(OH)2D3 increased between d0.5 and d2 in Primi-SGE, but remained unchanged in Primi-C, as did parathyroid hormone (PTH) and Ca in all Primi. Urinary Ca of Primi-SGE was increased on d2, indicating regulation of Ca excess. Three Multi-C cows with confirmed clinical hypocalcaemia needed treatment and thus were excluded from the dataset and replaced. Blood serum 1,25(OH)2D3 and PTH increased while Ca dropped by 40% between d0.5 and d2 in Multi-C, whereas 1,25(OH)2D3, Ca and PTH remained unchanged in Multi-SGE. Blood serum carboxyterminal telopeptide of type I collagen was higher in Primi than in Multi and increased with time, except in Primi-C. Mineral contents in colostrum, milk and blood serum of calves were not influenced to a relevant degree. In conclusion, Primi-C did not, in contrast to Multi-C, develop subclinical hypocalcaemia (<2.0 mmol Ca/l). Prevention of hypocalcaemia with one SGE bolus applied three to four days prior to expected calving was successful in maintaining blood Ca within normal range in Multi over the critical first two days and up to the first three weeks of lactation, without any observed detrimental effects on cows or calves

Magnetohydrodynamic flow in a mock-up of a HCLL blanket. Part II: Experiments

Magnetohydrodynamische Strömungen im Modellexperiment eines HCLL Blankets Teil II Experimente in einem homogenen Magnetfeld In einem skalierten Modell eines Helium-gekühlten Blei-LithiumTestblanketmoduls für ITER wurden magnetohydrodynamische Flüssigmetall-Strömungen untersucht. Die Ergebnisse untermauern das untersuchte Designkonzept und dienen zur Validierung von numerischen Berechnungsverfahren. Die untersuchte Modellgeometrie wurde entsprechend einem bei der CEA entwickelten Design eines Europäischen Konzepts eines Flüssigmetall-Blankets für ITER gefertigt (Rampal et al. (2005)). In diesem Entwurf dient die eutektische Flüssigmetalllegierung PbLi als Brutmaterial. Die im Blanket freigesetzte thermische Leistung wird durch Helium abgeführt, das bei hohen Drücken und Geschwindigkeiten in engen Kanälen innerhalb der Wände und in Kühlplatten strömt. Die Originalgeometrie wurde um einen Faktor 2 verkleinert, damit das Experiment im großen Magneten des MEKKA Labors im Forschungszentrum Karlsruhe Platz findet, wo NaK als Modellfluid eingesetzt wird. Auf der Oberfläche des Modells wurden Druck- und Potentialverteilungen für eine Vielzahl von Parameterkombinationen gemessen. Als dimensionslose Größen zur Quantifizierung der Magnetfeldstärke und des Volumenstroms dienen die Hartmann - Zahl Ha und die Reynolds - Zahl Re. Die experimentellen Ergebnisse bestätigen theoretische Vorhersagen, wonach die größten Druckverluste in den Zu- und Abflussleitungen und in den poloidalen Verteilern und Sammlern entstehen. Zusätzliche kleinere Beiträge ergeben sich, wenn die Strömung durch schmale Öffnungen in der Rückwand und nahe der ersten Wand hindurch tritt. Im Vergleich mit diesen Anteilen ist der Druckverlust in den Bruteinheiten bei Strömungsgeschwindigkeiten von 1-2mm/s vernachlässigbar klein. Aus dem weiten Bereich der untersuchten Parameter, 500 ≤ Ha ≤ 5000, 200 &lt; Re &lt; 10000, kann eine Druckverlust-Korrelation abgeleitet werden, mit deren Hilfe die Ergebnisse auf ITER oder DEMO Bedingungen extrapoliert werden können. Aus Messungen des elektrischen Potentials auf der Oberfläche des Moduls kann die Strömungsverteilung zwischen den Kühlplatten ermittelt werden. Trotz der komplexen Geometrie findet man für starke Magnetfelder und moderate Volumenströme eine recht gute Übereinstimmung mit Ergebnissen numerischer Berechnungen, die die volle elektrische Kopplung zwischen Unterkanälen und Bruteinheiten für eingelaufene Bedingungen in einer Mittelebene des Blankets berücksichtigen. Die starke elektromagnetische Kopplung führt zu einer hinreichend gleichförmigen Strömungsverteilung innerhalb der Bruteinheiten, mit etwas erhöhten Durchsätzen in den äußeren Kanälen entlang den sogenannten Grid Plates. Trägheitseffekte treten verstärkt bei schwächeren Magnetfeldern oder höheren Strömungsgeschwindigkeiten in Erscheinung. Für solche Bedingungen, bei kleineren Hartmann - Zahlen Ha und großen Reynolds - Zahlen Re, wird die Strömungsverteilung in den Bruteinheiten unsymmetrisch und es entstehen Gebiete mit Rückströmung oder geschlossene Rezirkulationsgebiete

Modification of turbulent dissipation rates by a deep Southern Ocean eddy

The impact of a mesoscale eddy on the magnitude and spatial distribution of diapycnal ocean mixing is investigated using a set of hydrographic and microstructure measurements collected in the Southern Ocean. These data sampled a baroclinic, mid-depth eddy formed during the disintegration of a deep boundary current. Turbulent dissipation is suppressed within the eddy, but is elevated by up to an order of magnitude along the upper and lower eddy boundaries. A ray-tracing approximation is employed asa heuristic device to elucidate how the internal wave field evolves in the ambient velocity and stratification conditions accompanying the eddy. These calculations are consistent with the observations, suggesting reflection of internal wave energy from the eddy center and enhanced breaking through critical layer processes along the eddy boundaries. These results have important implications for understanding where and how internal wave energy is dissipated in the presence of energetic deep geostrophic flows

The barrel DIRC of PANDA

Cooled antiproton beams of unprecedented intensities in the momentum range of 1.5-15 GeV/c will be used for the PANDA experiment at FAIR to perform high precision experiments in the charmed quark sector. The PANDA detector will investigate antiproton annihilations with beams in the momentum range of 1.5 GeV/c to 15 GeV/c on a fixed target. An almost 4π acceptance double spectrometer is divided in a forward spectrometer and a target spectrometer. The charged particle identification in the latter is performed by ring imaging Cherenkov counters employing the DIRC principle

The barrel DIRC of PANDA

Cooled antiproton beams of unprecedented intensities in the momentum range of 1.5-15 GeV/c will be used for the PANDA experiment at FAIR to perform high precision experiments in the charmed quark sector. The PANDA detector will investigate antiproton annihilations with beams in the momentum range of 1.5 GeV/c to 15 GeV/c on a fixed target. An almost 4π acceptance double spectrometer is divided in a forward spectrometer and a target spectrometer. The charged particle identification in the latter is performed by ring imaging Cherenkov counters employing the DIRC principle

The first XMM-Newton spectrum of a high redshift quasar - PKS 0537-286

We present XMM-Newton observations of the high redshift z=3.104, radio-loud quasar PKS 0537-286. The EPIC CCD cameras provide the highest signal-to-noise spectrum of a high-z quasar to date. The EPIC observations show that PKS 0537-286 is extremely X-ray luminous (Lx=2x10^47 erg/s), with an unusually hard X-ray spectrum (Gamma=1.27+/-0.02). The flat power-law emission extends over the whole observed energy range; there is no evidence of intrinsic absorption, which has been claimed in PKS 0537-286 and other high z quasars. However, there is evidence for weak Compton reflection. A redshifted iron K line, observed at 1.5 keV - corresponding to 6.15 keV in the quasar rest frame - is detected at 95% confidence. If confirmed, this is the most distant iron K line known. The line equivalent width is small (33eV), consistent with the `X-ray Baldwin effect' observed in other luminous quasars. The reflected continuum is also weak (R=0.25). We find the overall spectral energy distribution of PKS 0537-286 is dominated by the X-ray emission, which, together with the flat power-law and weak reflection features, suggests that the X-radiation from PKS 0537-286 is dominated by inverse Compton emission associated with a face-on relativistic jet.Comment: 6 pages, accepted for publication in Astronomy and Astrophysics Letter

m6A RNA methylation of major satellite repeat transcripts facilitates chromatin association and RNA:DNA hybrid formation in mouse heterochromatin

Heterochromatin has essential functions in maintaining chromosome structure, in protecting genome integrity and in stabilizing gene expression programs. Heterochromatin is often nucleated by underlying DNA repeat sequences, such as major satellite repeats (MSR) and long interspersed nuclear elements (LINE). In order to establish heterochromatin, MSR and LINE elements need to be transcriptionally competent and generate non-coding repeat RNA that remain chromatin associated. We explored whether these heterochromatic RNA, similar to DNA and histones, may be methylated, particularly for 5-methylcytosine (5mC) or methyl-6-adenosine (m6A). Our analysis in mouse ES cells identifies only background level of 5mC but significant enrichment for m6A on heterochromatic RNA. Moreover, MSR transcripts are a novel target for m6A RNA modification, and their m6A RNA enrichment is decreased in ES cells that are mutant for Mettl3 or Mettl14, which encode components of a central RNA methyltransferase complex. Importantly, MSR transcripts that are partially deficient in m6A RNA methylation display impaired chromatin association and have a reduced potential to form RNA:DNA hybrids. We propose that m6A modification of MSR RNA will enhance the functions of MSR repeat transcripts to stabilize mouse heterochromatin

Status of the PANDA barrel DIRC

The PANDA experiment at the future Facility for Antiproton and Ion Research in Europe GmbH (FAIR) at GSI, Darmstadt will study fundamental questions of hadron physics and QCD using high-intensity cooled antiproton beams with momenta between 1.5 and 15 GeV/c. Hadronic PID in the barrel region of the PANDA detector will be provided by a DIRC (Detection of Internally Reflected Cherenkov light) counter. The design is based on the successful BABAR DIRC with several key improvements, such as fast photon timing and a compact imaging region. Detailed Monte Carlo simulation studies were performed for DIRC designs based on narrow bars or wide plates with a variety of focusing solutions. The performance of each design was characterized in terms of photon yield and single photon Cherenkov angle resolution and a maximum likelihood approach was used to determine the π/K separation. Selected design options were implemented in prototypes and tested with hadronic particle beams at GSI and CERN. This article describes the status of the design and R&#38;D for the PANDA Barrel DIRC detector, with a focus on the performance of different DIRC designs in simulation and particle beams

Thermodynamic Properties of Spin Ladders with Cyclic Exchange

By high temperature series expansion and exact complete diagonalization the magnetic susceptibility chi(T) and the specific heat C(T) of a two-leg S=1/2 ladder with cyclic (4-spin) exchange are computed. Both methods yield convincing results for not too small temperatures. We find that a small amount of cyclic exchange influences the thermodynamical properties significantly. Our results can serve as reliable basis for an efficient analysis of experimental dataComment: 6 pages, 7 figure