Identification of differentially expressed genes in pathways of cerebral neurotransmission of anovulatory mice

Polycystic ovary syndrome is the classic example of loss of functional cyclicity and anomalous feedback. In this case, the excessive extra-glandular production and conversion of androgens to estrogens are the pathophysiological basis of the chronic anovulation. The literature describes an experimental model of the polymicrocystic ovary in obese diabetic mice with insulin resistance. The fact that these animals exhibit obesity, insulin resistance, and infertility demonstrates their skill as an experimental model for polycystic ovary. A recent study using long protocol for up to 40 weeks showed that anovulatory and obese mice transplanted with adipose tissue from animals with normal weight have multiple changes in their phenotype. These changes include reduction of body weight, prevention of obesity, insulin level normalization, and insulin tolerance tests, preventing the elevation of steroids and especially the reversal of fertility restoration with anovulation. Considering that there are close relationships between the ovulation process and the central nervous system, we propose to evaluate the gene expression levels of 84 different genes involved in neurotransmission and insulin pathways in addition to examining the neurolipidosis differential murine brain before and after reversal of anovulation. The present study showed changes in gene expression of molecular markers in brain tissue of animals for brain neurotransmission pathways as well as pathways for insulin. GABAergic genes, muscarinic, serotonin receptors, receptor tyrosine kinase, and genes of interleukin 6 showed overexpression profile. There was also a change in the lipid content in anovulatory brain, obesity, and insulin resistant mice (Ob-/Ob-) compared with controls. The re-introduction of leptin in these animals appears to reverse, at least in part, this profile.Univ Fed Sao Paulo, Lab Ginecol Mol, Sao Paulo, SP, BrazilUniv Fed Sao Paulo, Lab Ginecol Mol, Sao Paulo, SP, BrazilWeb of Scienc

Pressure and chemical substitution effects in the local atomic structure of BaFe2As2

The effects of K and Co substitutions and quasi-hydrostatic applied pressure (P<9 GPa) in the local atomic structure of BaFe2As2, Ba(Fe{0.937}Co{0.063})2As2 and Ba{0.85}K{0.15}Fe2As2 superconductors were investigated by extended x-ray absorption fine structure (EXAFS) measurements in the As K absorption edge. The As-Fe bond length is found to be slightly reduced (<~ 0.01 Angstroms) by both Co and K substitutions, without any observable increment in the corresponding Debye Waller factor. Also, this bond is shown to be compressible (k = 3.3(3)x10^{-3} GPa^{-1}). The observed contractions of As-Fe bond under pressure and chemical substitutions are likely related with a reduction of the local Fe magnetic moments, and should be an important tuning parameter in the phase diagrams of the Fe-based superconductors.Comment: 7 pages, 6 figure

Nefrite Túbulo-Intersticial Hipocomplementémica Idiopática

Background: Tubulointerstitial nephritis (TIN) is a common cause of kidney injury typically seen in association with drug exposure, infection or autoimmune diseases. However, TIN with interstitial immune complex deposition, without glomerular injury, is rarely observed. Case: We report a case of a 64-yearold Indian woman admitted for dialysis-requiring renal failure, without involvement of other organs. Urinalysis showed blood 3+ and 24h proteinuria of 1.5 g. Renal ultrasound revealed normal sized kidneys with loss of parenchymal-sinus differentiation. Laboratory tests disclosed low C3, positive ANA but negative anti-dsDNA, SSA and SSB. Serum protein electrophoresis was normal. The renal biopsy showed tubulointerstitial nephritis with positive immunoglobulin staining involving the interstitium and tubular basement membrane with glomerular sparing. The patient started prednisolone (1mg/kg/day) without recovery of the renal function. Conclusion: Idiopathic hypocomplementaemic tubulointerstitial nephritis is a rare disease with few cases described in the literature. To our knowledge this is the first case reported in Portugal

QTLs para características de crescimento numa população F2 de bovinos leiteiros.

O objetivo do presente estudo foi identificar quantitative trait loci (QTL) para características de crescimento nos cromossomos 1, 8, 10 e 23 numa população F2 Holandês x Gir. Vinte e quatro marcadores microssatélites foram selecionados a partir mapa genético de bovinos. Seis QTLs foram encontradaos em BTA-1, BTA-8, BTA-10 e BTA-23. No BTA-1, um QTL sugestivo foi detectado para o ganho de peso diário de um ano para dois anos (ADG365_720) em 12 cM. Em BTA 8, um QTL sugestivo foi identificada para o ganho de peso diário do peso ao nascimento e para 60 dias (ADG0_60) em 143 cM. Em BTA 10, três QTL sugestivo foi detectado para o peso em dois anos (W720), ADG365_720 e ganho médio diário (ADG total) em a região de 20 ? 22 cM com evidência de pleiotropia. Em BTA 23, um QTL sugestivo foi identificado para o ganho de peso diário de 60 dias para 205 dias (ADG60_205) em 19 cM. Nenhum outro efeito significativo QTL foi detectado para peso ao nascer, peso ao desmame, peso aos 205 dias e peso ao sobreano

Burden and severity of children's hospitalizations by respiratory syncytial virus in Portugal, 2015-2018

Background Respiratory syncytial virus (RSV) is a leading cause of acute lower respiratory infection (ALRI) in young children and is of considerable burden on healthcare systems. Our study aimed to evaluate ALRI hospitalizations related to RSV in children in Portugal. Methods We reviewed hospitalizations potentially related to RSV in children aged <5 years from 2015 to 2018, using anonymized administrative data covering all public hospital discharges in mainland Portugal. Three case definitions were considered: (a) RSV-specific, (b) (a) plus unspecified acute bronchiolitis (RSV-specific & Bronchiolitis), and (c) (b) plus unspecified ALRI (RSV-specific & ALRI). Results A total of 9697 RSV-specific hospitalizations were identified from 2015 to 2018—increasing to 26 062 for RSV-specific & ALRI hospitalizations—of which 74.7% were during seasons 2015/2016–2017/2018 (November–March). Mean hospitalization rates per season were, for RSV-specific, RSV-specific & Bronchiolitis, and RSV-specific & ALRI, respectively, 5.6, 9.4, and 11.8 per 1000 children aged <5 years and 13.4, 22.5, and 25.9 in children aged <2 years. Most RSV-specific hospitalizations occurred in healthy children (94.9%) and in children aged <2 years (96.3%). Annual direct costs of €2.4 million were estimated for RSV-specific hospitalizations—rising to €5.1 million for RSV-specific & ALRI—mostly driven by healthy children (87.6%). Conclusion RSV is accountable for a substantial number of hospitalizations in children, especially during their first year of life. Hospitalizations are mainly driven by healthy children. The variability of the potential RSV burden across case definitions highlights the need for a universal RSV surveillance system to guide prevention strategies

Interações e epistáticas entre QTLs afetando o peso aos dois anos em bovinos mestiços leiteiro.

O objetivo deste estudo foi detectar QTLs e epistasia afetando o peso de dois anos na população de mestiços de gado leiteiro. Dois QTLs sugestivos foram encontrados nos cromossomos BTA 2 e BTA 3 no modelo inclui o efeito aditivo e dominância, e três QTLs sugestivos foram detectados nos BTA 6, BTA 10 e BTA 12 no modelo inclui apenas efeito aditivo. Um QTL significativo foi detectado em BTA 22 no modelo inclui o efeito aditivo e dominância. Identificaram dez pares de genes epistáticos