Pterygium and Associated Ocular Surface Squamous Neoplasia

Objective: To measure the rate of histopathologically identified ocular surface squamous neoplasia (OSSN) in pterygium specimens. Methods: All pterygium specimens collected from consecutive patients between April 8, 2003, and February 6, 2008, were submitted for histopathologic examination, and the rate of OSSN was calculated. Results: The rate of OSSN was 9.8% (52 of 533) in sequential pterygium specimens. Conclusions: This rate of unsuspected OSSN suggests that all specimens of pterygium should be submitted for histopathologic examination and that patients in whom OSSN is noted should be examined at more frequent intervals so any clinical OSSN that develops can be identified at an early stage

IN SITU MEASUREMENTS OF THE ACOUSTIC TARGET STRENGTH OF CAPE HORSE MACKEREL TRACHURUS TRACHURUS CAPENSIS OFF NAMIBIA

The acoustic target strength (TS) of Cape horse mackerel Trachurus trachurus capensis was measured in situ at 38 kHz during two surveys over the Namibian continental shelf in 1998 and 1999 using a SIMRAD EK500 echosounder/ES38D submersible split-beam transducer. Scattered aggregations of horse mackerel 100–200 m deep were ensonified. The transducer was lowered to a depth of 85–140 m in order to resolve single targets at short ranges (5–50 m). Individual fish were tracked using specially developed software. Samples of ensonified fish were obtained using pelagic and demersal trawls; the former was fitted with a codend Multisampler for depth-specific sampling. Recorded TS estimates were low, producing b20-values ranging from -77.5 to -74.9 dB (-76.0 dB &#177 1.3), considerably lower than published estimates for horse mackerel (-73.4 dB &#60 b20 &#60 -65.2 dB). An explanation for the weak acoustic backscattering may be swimbladder compression. Surface-projected b20 values, which were computed using the depth of each target and the scattering area reduction rate previously found for herring (γ &#61 -0.29), corresponded to -72.6 dB. This value is close to the TS constant of -72 dB currently applied for horse mackerel in Namibian and Angolan waters.Afr. J. mar. Sci. 25: 239–25

Effects of once-weekly semaglutide on appetite, energy intake, control of eating, food preference and body weight in subjects with obesity.

AIM: To investigate the mechanism of action for body weight loss with semaglutide. MATERIALS AND METHODS: This randomised, double-blind, placebo-controlled, two-period crossover trial investigated the effects of 12 weeks treatment with once-weekly subcutaneous semaglutide, dose-escalated to 1.0 mg, in 30 subjects with obesity. Ad libitum energy intake, ratings of appetite, thirst, nausea and well-being, control of eating, food preference, resting metabolic rate, body weight and body composition were assessed. RESULTS: After a standardised breakfast, semaglutide, compared with placebo, led to a lower ad libitum energy intake during lunch (-1255 kJ; P < 0.0001), and during the subsequent evening meal (P = 0.0401) and snacks (P = 0.0034), resulting in a 24% reduction in total energy intake across all ad libitum meals throughout the day (-3036 kJ; P < 0.0001). Fasting overall appetite suppression scores were improved with semaglutide versus placebo, while nausea ratings were similar. Semaglutide was associated with less hunger and food cravings, better control of eating and a lower preference for high-fat foods. Resting metabolic rate, adjusted for lean body mass, did not differ between treatments. Semaglutide led to a reduction from baseline in mean body weight of 5.0 kg, predominantly from body fat mass. CONCLUSION: After 12 weeks' treatment, ad libitum energy intake was substantially lower with semaglutide versus placebo with a corresponding loss of body weight observed with semaglutide. In addition to reduced energy intake, likely mechanisms for semaglutide-induced weight loss included less appetite and food cravings, better control of eating and lower relative preference for fatty, energy-dense foods

Motivating doctors into leadership and management: a cross-sectional survey

Purpose: Calls for doctors to enter management are louder as the benefits of medical leadership become clearer. But supply is not meeting demand. This study asks doctors: what might encourage you to go into leadership, and what do you see as the disincentives? The same was asked about leadership training. First, the paper attempts to understand doctors’ motivation to lead, specifically, to explore the job characteristics that might act as incentives and disincentives. Second, the study points to organisational obstacles that further shrink the medical leadership pipeline. Method: Doctors were surveyed through the Organization of Danish Medical Societies. Our key variables included: 1) willingness to take on a management or leadership position; 2) the incentives to go into leadership or management; 3) disincentives to do so; and 4) incentives for participating in leadership training. Our sample includes 3534 doctors (17% response rate). Findings: Nearly 70% of doctors said that they would consider leadership or management positions. Overwhelmingly, the main incentive reported was to have a positive impact. Doctors are put off by fears of extra administration, longer hours, burnout, lack of resources, and by organisational cultures resistant to change. But they are fully aware of their need for leadership training. Practical implications: Health systems should adapt to reflect the motivations of their potential medical leaders, especially the best talent, who may not be the first to apply for management positions. It is also essential they offer leadership training. These findings, that aid succession planning, are especially important as more is known about the influence of medical leaders on organisational outcomes, and at a time of high reported stress, burnout, and staff recruitment and retention challenges

A P-type ATPase importer that discriminates between essential and toxic transition metals

Transition metals, although being essential cofactors in many physiological processes, are toxic at elevated concentrations. Among the membrane-embedded transport proteins that maintain appropriate intracellular levels of transition metals are ATP-driven pumps belonging to the P-type ATPase superfamily. These metal transporters may be differentiated according to their substrate specificities, where the majority of pumps can extrude either silver and copper or zinc, cadmium, and lead. In the present report, we have established the substrate specificities of nine previously uncharacterized prokaryotic transition-metal P-type ATPases. We find that all of the newly identified exporters indeed fall into one of the two above-mentioned categories. In addition to these exporters, one importer, Pseudomonas aeruginosa Q9I147, was also identified. This protein, designated HmtA (heavy metal transporter A), exhibited a different substrate recognition profile from the exporters. In vivo metal susceptibility assays, intracellular metal measurements, and transport experiments all suggest that HmtA mediates the uptake of copper and zinc but not of silver, mercury, or cadmium. The substrate selectivity of this importer ensures the high-affinity uptake of essential metals, while avoiding intracellular contamination by their toxic counterparts

Capabilitarian Sufficiency: Capabilities and Social Justice

This paper suggests an account of sufficientarianism—that is, that justice is fulfilled when everyone has enough—laid out within a general framework of the capability approach. In doing so, it seeks to show that sufficiency is especially plausible as an ideal of social justice when constructed around key capabilitarian insights such as freedom, pluralism, and attention to empirical interconnections between central capabilities. Correspondingly, we elaborate on how a framework for evaluating social justice would look when constructed in this way and give reasons for why capabilitarians should embrace sufficientarianism. We do this by elaborating on how capabilitarian values underpin sufficiency. On this basis, we identify three categories of central capabilities; those related to biological and physical needs, those to fundamental interests of a human agent, and those to fundamental interests of a social being. In each category, we argue, achieving sufficiency requires different distributional patterns depending on how the capabilities themselves work and interrelate. This argument adds a new dimension to the way capabilitarians think about social justice and changes how we should target instances of social justice from social-political viewpoint

Updates in Rhea-a manually curated resource of biochemical reactions.

Rhea (http://www.ebi.ac.uk/rhea) is a comprehensive and non-redundant resource of expert-curated biochemical reactions described using species from the ChEBI (Chemical Entities of Biological Interest) ontology of small molecules. Rhea has been designed for the functional annotation of enzymes and the description of genome-scale metabolic networks, providing stoichiometrically balanced enzyme-catalyzed reactions (covering the IUBMB Enzyme Nomenclature list and additional reactions), transport reactions and spontaneously occurring reactions. Rhea reactions are extensively curated with links to source literature and are mapped to other publicly available enzyme and pathway databases such as Reactome, BioCyc, KEGG and UniPathway, through manual curation and computational methods. Here we describe developments in Rhea since our last report in the 2012 database issue of Nucleic Acids Research. These include significant growth in the number of Rhea reactions and the inclusion of reactions involving complex macromolecules such as proteins, nucleic acids and other polymers that lie outside the scope of ChEBI. Together these developments will significantly increase the utility of Rhea as a tool for the description, analysis and reconciliation of genome-scale metabolic models

CYP83B1 Is the Oxime-metabolizing Enzyme in the Glucosinolate Pathway in \u3ci\u3eArabidopsis\u3c/i\u3e

CYP83B1 from Arabidopsis thaliana has been identified as the oxime-metabolizing enzyme in the biosynthetic pathway of glucosinolates. Biosynthetically active microsomes isolated from Sinapis alba converted p-hydroxyphenylacetaldoxime and cysteine into S-alkylated p-hydroxyphenylacetothiohydroximate, S-(p-hydroxyphenylacetohydroximoyl)-L-cysteine, the next proposed intermediate in the glucosinolate pathway. The production was shown to be dependent on a cytochrome P450 monooxygenase. We searched the genome of A. thaliana for homologues of CYP71E1 (P450ox), the only known oxime-metabolizing enzyme in the biosynthetic pathway of the evolutionarily related cyanogenic glucosides. By a combined use of bioinformatics, published expression data, and knock-out phenotypes, we identified the cytochrome P450 CYP83B1 as the oxime-metabolizing enzyme in the glucosinolate pathway as evidenced by characterization of the recombinant protein expressed in Escherichia coli. The data are consistent with the hypothesis that the oxime-metabolizing enzyme in the cyanogenic pathway (P450ox) was mutated into a “P450mox” that converted oximes into toxic compounds that the plant detoxified into glucosinolates

Updates in Rhea - an expert curated resource of biochemical reactions.

Rhea (http://www.rhea-db.org) is a comprehensive and non-redundant resource of expert-curated biochemical reactions designed for the functional annotation of enzymes and the description of metabolic networks. Rhea describes enzyme-catalyzed reactions covering the IUBMB Enzyme Nomenclature list as well as additional reactions, including spontaneously occurring reactions, using entities from the ChEBI (Chemical Entities of Biological Interest) ontology of small molecules. Here we describe developments in Rhea since our last report in the database issue of Nucleic Acids Research. These include the first implementation of a simple hierarchical classification of reactions, improved coverage of the IUBMB Enzyme Nomenclature list and additional reactions through continuing expert curation, and the development of a new website to serve this improved dataset