Nanoparticle behaviour in an urban street canyon at different heights and implications on indoor respiratory doses

The amount of outdoor particles that indoor environments receive depends on the particle infiltration factors (Fin), peculiar of each environment, and on the outdoor aerosol concentrations and size distributions. The respiratory doses received, while residing indoor, will change accordingly. This study aims to ascertain to what extent such doses are affected by the vertical distance from the traffic sources. Particle number size distributions have been simultaneously measured at street level and at about 20 m height in a street canyon in downtown Rome. The same Fin have been adopted to estimate indoor aerosol concentrations, due to the infiltration of outdoor particles and then the relevant daily respiratory doses. Aerosol concentrations at ground floor were more than double than at 20 m height and richer in ultrafine particles. Thus, although aerosol infiltration efficiency was on average higher at 20 m height than at ground floor, particles more abundantly infiltrated at ground level. On a daily basis, this involved a 2.5-fold higher dose at ground level than at 20 m height. At both levels, such doses were greater than those estimated over the period of activity of some indoor aerosol sources; therefore, they represent an important contribution to the total daily dose

A method validation for simultaneous determination of phthalates and bisphenol A released from plastic water containers

Phthalates (or phthalate esters, PAEs) and bisphenol A (BPA) are widely used in various industries, particularly in the fields of cosmetics and packaging, and they increase the malleability and workability of materials. As a result of their use, some international health organizations have begun to study them. In this study, the authors developed a methodology for the simultaneous determination of dimethyl phthalate (DMP), diethyl phthalate (DEP), diisobutyl phthalate (DiBP); dibutyl phthalate (DBP), bis(2-ethylhexyl) phthalate (DEHP); di-n-octyl-phthalate (DnOP) and bisphenol A (BPA) from drinking and non-potable waters. The extraction of PAEs and BPA was performed using a solvent-based dispersive liquid-liquid microextraction (SB-DLLME) method. The analytical determination was performed using a gas chromatography-ion trap mass spectrometry (GC-IT/MS) analysis. The entire procedure was validated as recoveries were studied according to the volume and the extraction solvent used, pH, and ionic strength. Dynamic linearity ranges and linear equations of all the compounds were experimentally determined as well as the limit of detection (LOD) (1-8 ng mL-1) and the limit of quantification (LOQ) (5-14 ng mL-1), reproducibility, and sensitivity. The method was applied to 15 water samples (mineral water and tap water) for determining PAEs and BPA released from the plastic container. After the release simulation, four PAEs (i.e., DiBP, DBP, DHEP, and DnOP) were determined at very low concentrations (below 1.2 ng mL-1) in two water samples from (sport) bottles

Escaping Antiangiogenic Therapy: Strategies Employed by Cancer Cells

Indexación: Web of ScienceTumor angiogenesis is widely recognized as one of the hallmarks of cancer. Consequently, during the last decades the development and testing of commercial angiogenic inhibitors has been a central focus for both basic and clinical cancer research. While antiangiogenic drugs are now incorporated into standard clinical practice, as with all cancer therapies, tumors can eventually become resistant by employing a variety of strategies to receive nutrients and oxygen in the event of therapeutic assault. Herein, we concentrate and review in detail three of the principal mechanisms of antiangiogenic therapy escape: (1) upregulation of compensatory/alternative pathways for angiogenesis; (2) vasculogenic mimicry; and (3) vessel co-option. We suggest that an understanding of how a cancer cell adapts to antiangiogenic therapy may also parallel the mechanisms employed in the bourgeoning tumor and isolated metastatic cells delivering responsible for residual disease. Finally, we speculate on strategies to adapt antiangiogenic therapy for future clinical uses.http://www.mdpi.com/1422-0067/17/9/148

Monoaromatic compounds in ambient air of various cities: A focus on correlations between the xylenes and ethylbenzene

Speciation of o-xylene, m-xylene, p-xylene and ethylbenzene was performed by gas chromatography from ambient air and liquid fuel samples collected at various locations in 19 cities in Europe, Asia and South America. The xylene's mixing ratios were compared to each other from the various locations, which included urban air, traffic air and liquid fuel. For all samples, the xylenes exhibited robust correlations, and the slopes remained constant. The m-xylene/p-xylene ratio was found to be 2.33±0.30, and the m-xylene/o-xylene ratio was found to be 1.84±0.25. These ratios remain persistent even in biomass combustion experiments (in South America and South Africa). Comparing the xylenes to toluene and benzene indicate that combustion, but not fuel evaporation, is the major common source of the xylenes in areas dominated by automotive emissions. Although a wide range of combustion types and combustion efficiencies were encountered throughout all the locations investigated, xylenes and ethylbenzene ratios remained persistent. We discuss the implications of the constancies in the xylenes and ethylbenzene ratios on atmospheric chemistry

Probing the Ultimate Limit of Fiber-Optic Strain Sensing

Enhanced Strain Sensitivity The ability to measure tiny deformations in length is useful for many disciplines, from largescale structural engineering to DNA analysis with optical tweezers. The most sensitive strain sensors are those using optical interferometers, which can detect small changes at the scale of visible wavelengths. Using an optical frequency comb to stabilize the output of a diode laser, and as a highly accurate ruler to determine small changes in length of an optic fiber sensor, Gagliardi et al. (p. 1081 , published online 28 October) showed that sensitivity can be enhanced by several orders of magnitude. Such combined technology should provide for a new generation of high-performance sensors

Oxidative potential associated with urban aerosol deposited into the respiratory system and relevant elemental and ionic fraction contributions

Size-segregated aerosol measurements were carried out at an urban and at an industrial site. Soluble and insoluble fractions of elements and inorganic ions were determined. Oxidative potential (OP) was assessed on the soluble fraction of Particulate Matter (PM) by ascorbic acid (AA), dichlorofluorescein (DCFH) and dithiothreitol (DTT) assays. Size resolved elemental, ion and OP doses in the head (H), tracheobronchial (TB) and alveolar (Al) regions were estimated using the Multiple-Path Particle Dosimetry (MPPD) model. The total aerosol respiratory doses due to brake and soil resuspension emissions were higher at the urban than at the industrial site. On the contrary, the doses of anthropic combustion tracers were generally higher at the industrial site. In general, the insoluble fraction was more abundantly distributed in the coarse than in the fine mode and vice versa for the soluble fraction. Consequently, for the latter, the percent of the total respiratory dose deposited in TB and Al regions increased. Oxidative potential assay (OPAA) doses were distributed in the coarse region; therefore, their major contribution was in the H region. The contribution in the TB and Al regions increased for OPDTT and OPDCFH

May SARS-CoV-2 diffusion be favored by alkaline aerosols and ammonia emissions?

Ammonia is a common factor linking air in bat caves and air pollution in the proximity of agricultural fields treated with livestock farming sewage and slaughterhouses, where important clusters of COVID-19 have recently been reported all over the world. Such a commonality has a further connection with the known behavior of some viruses of the coronavirus family, such as the murine hepatitis virus, whose spike glycoprotein (S) can be triggered to a membrane-binding conformation at pH 8.0. Within the airborne route of virus transmission, with particular relevance for crowded and enclosed environments, these observations have prompted a hypothesis that may represent a contributing cause to interpret the geographical variability of the virus diffusion and the surging rise of COVID-19 cases in slaughterhouses all over the world. The hypothesis is that, in these environments, the SARS-CoV-2 S protein may find on a fraction of the airborne particles an alkaline pH, favorable to trigger the conformational changes, needed to induce the fusion of the viral envelope with the plasma membrane of the target cells

Investigating the resonance spectrum of optical frequency combs in fiber-optic cavities

We report a detailed theoretical and experimental study of fiberoptic cavities under broadband excitation by mode-locked laser combs. We calculate the effects of fiber dispersion on the cavity transmission. For any integer ratio between the comb repetition rate and cavity free spectral range, the theoretical resonant output spectrum exhibits a narrow group of resonant teeth, surrounded by minor, unevenly spaced resonances. Also, the central resonance can be rapidly and precisely tuned over the entire comb span by only acting on its repetition rate. Experimental observations are provided by a single-mode fiber ring and a telecom-wavelength comb laser. The resulting spectral pattern agrees very well with our theoretical prediction, allowing a thorough characterization of the cavity dispersion and opening new perspectives for comb spectroscopy in dielectric resonators. © 2013 Optical Society of America

Localized strain sensing with fiber Bragg-grating ring cavities

We report the theoretical description and the experimental demonstration of an optical resonator formed by inserting a Fiber Bragg Grating (FBG) in a closed fiber loop. The spectral characteristics of such a resonator strongly depend on the reflectivity of the FBG. In the wavelength region where the FBG reflectivity R is negligible, the system behaves like a conventional ring resonator. On the other hand, when R is not vanishing, a split-mode structure can be observed, associated to the degeneracy removal of two counterpropagating resonant modes. The magnitude of the mode splitting can be used to sense small variations of the FBG physical parameters, such as length, temperature or group index. An example of strain sensing with this setup is reported, showing that the mode splitting is sensitive to a mechanical strain applied to the FBG, while it is almost insensitive to a strain applied to any other point of the resonator. This peculiar feature allows to perform cavity-enhanced, local strain measurements with a reduced sensitivity to environmental perturbations, which represents an important improvement in the framework of the fiber-optic sensors. (C)2013 Optical Society of Americ

A MEC-based Extended Virtual Sensing for Automotive Services

Multi-access edge computing (MEC) comes with the promise of enabling low-latency applications and of reducing core network load by offloading traffic to edge service instances. Recent standardization efforts, among which the ETSI MEC, have brought about detailed architectures for the MEC. Leveraging the ETSI model, in this paper we first present a flexible, yet full-fledged, MEC architecture that is compliant with the standard specifications. We then use such architecture, along with the popular OpenAir Interface (OAI), for the support of automotive services with very tight latency requirements. We focus in particular on the Extended Virtual Sensing (EVS) services, which aim at enhancing the sensor measurements aboard vehicles with the data collected by the network infrastructure, and exploit this information to achieve better safety and improved passengers/driver comfort. For the sake of concreteness, we select the intersection control as an EVS service and present its design and implementation within the MEC platform. Experimental measurements obtained through our testbed show the excellent performance of the MEC EVS service against its equivalent cloud-based implementation, proving the need for MEC to support critical automotive services, as well as the benefits of the solution we designed.This work was supported by the European Commission through the H2020 5G-TRANSFORMER project (Project ID 761536). The work of Christian Vitale was also supported by the European Union’s Horizon 2020 Research and Innovation Programme under Grant 739551 (KIOS CoE) and from the Republic of Cyprus through the Directorate General for Euro-pean Programmes, Coordination, and Development