Inadequate food intake at high temperatures is related to depressed mitochondrial respiratory capacity

Animals, especially ectotherms, are highly sensitive to the temperature of their surrounding environment. Extremely high temperature, for example, induces a decline of average performance of conspecifics within a population, but individual heterogeneity in the ability to cope with elevating temperatures has rarely been studied. In this study, we examined inter-individual variation in feeding ability and consequent growth rate of juvenile brown trout Salmo trutta acclimated to a high temperature (19°C), and investigated the relationship between these metrics of whole-animal performances and among-individual variation in mitochondrial respiration capacity. Food was provided ad libitum yet intake varied ten-fold amongst individuals, resulting in some fish losing weight whilst others continued to grow. Almost half of the variation in food intake was related to variability in mitochondrial capacity: low intake (and hence growth failure) was associated with high leak respiration rates within liver and muscle mitochondria, and a lower coupling of muscle mitochondria. These observations, combined with the inability of fish with low food consumption to increase their intake despite ad libitum food levels, suggest a possible insufficient capacity of the mitochondria for maintaining ATP homeostasis. Individual variation in thermal performance is likely to confer variation in the upper limit of an organism's thermal niche and in turn affect the structure of wild populations in warming environments

Differential effects of food availability on minimum and maximum rates of metabolism

Metabolic rates reflect the energetic cost of living but exhibit remarkable variation among conspecifics, partly as a result of the constraints imposed by environmental conditions. Metabolic rates are sensitive to changes in temperature and oxygen availability, but effects of food availability, particularly on maximum metabolic rates, are not well understood. Here, we show in brown trout (Salmo trutta) that maximum metabolic rates are immutable but minimum metabolic rates increase as a positive function of food availability. As a result, aerobic scope (i.e. the capacity to elevate metabolism above baseline requirements) declines as food availability increases. These differential changes in metabolic rates likely have important consequences for how organisms partition available metabolic power to different functions under the constraints imposed by food availability

Direct Visualization of Single Nuclear Pore Complex Proteins Using Genetically-Encoded Probes for DNA-PAINT

The nuclear pore complex (NPC) is one of the largest and most complex protein assemblies in the cell and, among other functions, serves as the gatekeeper of nucleocytoplasmic transport. Unraveling its molecular architecture and functioning has been an active research topic for decades with recent cryogenic electron microscopy and super-resolution studies advancing our understanding of the architecture of the NPC complex. However, the specific and direct visualization of single copies of NPC proteins is thus far elusive. Herein, we combine genetically-encoded self-labeling enzymes such as SNAP-tag and HaloTag with DNA-PAINT microscopy. We resolve single copies of nucleoporins in the human Y-complex in three dimensions with a precision of circa 3 nm, enabling studies of multicomponent complexes on the level of single proteins in cells using optical fluorescence microscopy

Avaliação do crescimento micelial de Cylindrocladium candelabrum sob várias temperaturas.

Edição dos resumos do 35º Congresso Paulista de Fitopatologia, 2012, Jaguariúna

Incidência e severidade da mancha causada por Cylindrocladium candelabrum em árvores jovens de Eucalyptus benthamii em Santa Catarina.

Edição dos resumos do 35º Congresso Paulista de Fitopatologia, 2012, Jaguariúna

Levantamento de doenças em mudas de Eucalyptus benthamii em Santa Catarina.

Edição dos resumos do 35º Congresso Paulista de Fitopatologia, 2012, Jaguariúna

The Zeeman effect in the G band

We investigate the possibility of measuring magnetic field strength in G-band bright points through the analysis of Zeeman polarization in molecular CH lines. To this end we solve the equations of polarized radiative transfer in the G band through a standard plane-parallel model of the solar atmosphere with an imposed magnetic field, and through a more realistic snapshot from a simulation of solar magneto-convection. This region of the spectrum is crowded with many atomic and molecular lines. Nevertheless, we find several instances of isolated groups of CH lines that are predicted to produce a measurable Stokes V signal in the presence of magnetic fields. In part this is possible because the effective Land\'{e} factors of lines in the stronger main branch of the CH A$^{2}\Delta$--X$^{2}\Pi$ transition tend to zero rather quickly for increasing total angular momentum $J$, resulting in a Stokes $V$ spectrum of the G band that is less crowded than the corresponding Stokes $I$ spectrum. We indicate that, by contrast, the effective Land\'{e} factors of the $R$ and $P$ satellite sub-branches of this transition tend to $\pm 1$ for increasing $J$. However, these lines are in general considerably weaker, and do not contribute significantly to the polarization signal. In one wavelength location near 430.4 nm the overlap of several magnetically sensitive and non-sensitive CH lines is predicted to result in a single-lobed Stokes $V$ profile, raising the possibility of high spatial-resolution narrow-band polarimetric imaging. In the magneto-convection snapshot we find circular polarization signals of the order of 1% prompting us to conclude that measuring magnetic field strength in small-scale elements through the Zeeman effect in CH lines is a realistic prospect.Comment: 22 pages, 6 figures. To be published in the Astrophysical Journa

GEOS-5 Chemistry Transport Model User's Guide

The Goddard Earth Observing System version 5 (GEOS-5) General Circulation Model (GCM) makes use of the Earth System Modeling Framework (ESMF) to enable model configurations with many functions. One of the options of the GEOS-5 GCM is the GEOS-5 Chemistry Transport Model (GEOS-5 CTM), which is an offline simulation of chemistry and constituent transport driven by a specified meteorology and other model output fields. This document describes the basic components of the GEOS-5 CTM, and is a user's guide on to how to obtain and run simulations on the NCCS Discover platform. In addition, we provide information on how to change the model configuration input files to meet users' needs

The optimal combination of standard metabolic rate and aerobic scope for somatic growth depends on food availability

Metabolic rates can vary as much as threefold among individuals of the same size and age in a population, but why such variation persists is unclear given that they determine the energetic cost of living. Relationships between standard metabolic rate (SMR), growth and survival can vary with environmental conditions, suggesting that the fitness consequences of a given metabolic phenotype may be context-dependent. Less attention has focused on the link between absolute aerobic scope (AS, the difference between standard and maximum metabolic rate) and fitness under different environmental conditions, despite the importance of aerobic scope to an organism's total energetic capacity.<p></p> We examined the links between individual variation in both SMR and AS and somatic growth rates of brown trout (Salmo trutta) under different levels of food availability.<p></p> Standard metabolic rate and AS were uncorrelated across individuals. However, SMR and AS not only had interactive effects on growth, but these interactions depended on food level: at ad libitum food levels, AS had a positive effect on growth whose magnitude depended on SMR; at intermediate food levels, AS and SMR had interactive effects on growth, but at the low food level, there was no effect of either AS or SMR on growth. As a result, there was no metabolic phenotype that performed best or worst across all food levels.<p></p> These results demonstrate the importance of aerobic scope in explaining somatic growth rates and support the hypothesis that links between individual variation in metabolism and fitness are context-dependent.<p></p> The larger metabolic phenotype and the environmental context in which performance is evaluated both need to be considered in order to better understand the link between metabolic rates and fitness and thereby the persistence of individual variation in metabolic rates.<p></p&gt

Systematic study of carrier correlations in the electron-hole recombination dynamics of quantum dots

The ground state carrier dynamics in self-assembled (In,Ga)As/GaAs quantum dots has been studied using time-resolved photoluminescence and transmission. By varying the dot design with respect to confinement and doping, the dynamics is shown to follow in general a non-exponential decay. Only for specific conditions in regard to optical excitation and carrier population, for example, the decay can be well described by a mono-exponential form. For resonant excitation of the ground state transition a strong shortening of the luminescence decay time is observed as compared to the non-resonant case. The results are consistent with a microscopic theory that accounts for deviations from a simple two-level picture.Comment: 8 pages, 7 figure