726 research outputs found

    Immune responses in lactating Holstein cows supplemented with Cu, Mn, and Zn as sulfates or methionine hydroxy analogue chelates

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    The aim of this study was to compare effects of inorganic sulfate versus chelated forms of supplemental Cu, Mn, and Zn on milk production, plasma and milk mineral concentrations, neutrophil activity, and antibody titer response to a model vaccination. Holstein cows (n = 25) were assigned in 2 cohorts based on calving date to a 12-wk randomized complete block design study. The first cohort consisted of 17 cows that had greater days in milk (DIM; mean of 77 DIM at the start of the trial) than the second cohort of 8 cows (32 DIM at the start of the trial). Diets were formulated to supplement 100% of National Research Council requirements of Cu, Mn, and Zn by either inorganic trace minerals (ITM) in sulfate forms or chelated trace minerals (CTM) supplied as metal methionine hydroxy analog chelates, without accounting for trace mineral contribution from other dietary ingredients. Intake and milk production were recorded daily. Milk composition was measured weekly, and milk Cu, Mn, and Zn were determined at wk 0 and 8. Plasma Cu and Zn concentrations and neutrophil activity were measured at wk 0, 4, 8, and 12. Neutrophil activity was measured by in vitro assays of chemotaxis, phagocytosis, and reactive oxygen species production. A rabies vaccination was administered at wk 8, and vaccine titer response at wk 12 was measured by both rapid fluorescent focus inhibition test and ELISA. Analyzed dietary Cu was 21 and 23 mg/kg, Mn was 42 and 46 mg/kg, and Zn was 73 and 94 mg/ kg for the ITM and CTM diets, respectively. No effect of treatment was observed on milk production, milk composition, or plasma minerals. Dry matter intake was reduced for CTM compared with ITM cows, but this was largely explained by differences in body weight between treatments. Milk Cu concentration was greater for CTM than ITM cows, but this effect was limited to the earlier DIM cohort of cows and was most pronounced for multiparous compared with primiparous cows. Measures of neutrophil function were unaffected by treatment except for an enhancement in neutrophil phagocytosis with the CTM treatment found for the later DIM cohort of cows only. Rabies antibody titer in CTM cows was 2.8 fold that of ITM cows as measured by ELISA, with a trend for the rapid fluorescent focus inhibition test. Supplementation of Cu, Mn, and Zn as chelated sources may enhance immune response of early lactation dairy cows compared with cows supplemented with inorganic sources

    A comparative evaluation of modern English corpus grammatical annotation schemes

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    Many English Corpus Linguistics projects reported in ICAME Journal and elsewhere involve grammatical analysis or tagging of English texts (eg Atwell 1983, Leech et al 1983, Booth 1985, Owen 1987, Souter 1989a, O’Donoghue 1991, Belmore 1991, Kytö and Voutilainen 1995, Aarts 1996, Qiao and Huang 1998). Each new project has to review existing tagging schemes, and decide which to adopt and/or adapt. The AMALGAM project can help in this decision, by providing descriptions and analyses of a range of tagging schemes, and an internet-based service for researchers to try out the range of tagging schemes on their own data. The project AMALGAM (Automatic Mapping Among Lexico-Grammatical Annotation Models) explored a range of Part-of-Speech tagsets and phrase structure parsing schemes used in modern English corpus-based research. The PoS-tagging schemes include: Brown (Greene and Rubin 1981), LOB (Atwell 1982, Johansson et al 1986), Parts (man 1986), SEC (Taylor and Knowles 1988), POW (Souter 1989b), UPenn (Santorini 1990), LLC (Eeg-Olofsson 1991), ICE (Greenbaum 1993), and BNC (Garside 1996). The parsing schemes include some which have been used for hand annotation of corpora or manual post-editing of automatic parsers, and others which are unedited output of a parsing program. Project deliverables include: – a detailed description of each PoS-tagging scheme, at a comparable level of detail. This includes a list of PoS-tags with descriptions and example uses from the source Corpus. The description of the use of PoS-tags is also illustrated in a multi-tagged corpus: a set of sample texts PoS-tagged in parallel with each PoS-tagset (and proofread by experts), for comparative studies – an analysis of the different lexical tokenization rules used in the source Corpora, to arrive at a ‘Corpus-neutral’ tokenization scheme (and consequent adjustments to the PoS-tagsets in our study to accept modified tokenization) – an implementation of each PoS-tagset in conjunction with our standardised tokenizer, as a family of PoS-taggers, one for each PoS-tagset – a method for ‘PoS-tagset conversion’, taking a text tagged according to one PoS-tagset and outputting the text annotated with another PoS-tagset – a sample of texts parsed according to a range a parsing schemes: a Multi-Treebank resource for comparative studies – an Internet service allowing researchers worldwide free access to the above resources, including a simple email-based method for PoS-tagging any English text with any or all PoS-tagset(s)

    uvbyCaHbeta CCD Photometry of Clusters. V. The Metal-Deficient Open Cluster NGC 2243

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    CCD photometry on the intermediate-band uvbyCaHbeta system is presented for the metal-deficient open cluster, NGC 2243. Restricting the data to probable single members of the cluster using the CMD and the photometric indices alone generates a sample of 100 stars at the cluster turnoff. The average E(b-y) = 0.039 (0.003 s.e.m.) or E(B-V) = 0.055 (0.004 s.e.m.), where the errors refer to internal errors alone. With this reddening, [Fe/H] is derived from both m_1 and hk, using b-y and Hbeta as the temperature index. The agreement among the four approaches is excellent, leading to a final weighted average of [Fe/H] = -0.57 (0.03 s.e.m.) for the cluster, on a scale where the Hyades has [Fe/H] = +0.12. Using a combination of photometric and spectroscopic data, 27 probable cluster members are identified and used to delineate the red giant branch and a well-defined clump at V = 13.70, while eliminating the so-called second clump at V = 14.1. Interpolation between isochrones of appropriate [Fe/H] leads to an apparent modulus of (m-M) = 13.15 +/- 0.1 and an age of 3.8 +/- 0.2 Gyr. A differential CMD comparison with Berkeley 29, a cluster with a galactocentric distance almost twice that of NGC 2243, constrains Berkeley 29 to be at least as young and as metal-rich as NGC 2243.Comment: 21 pg postscript, latex to preprint format, 15 eps figures, 2 tables. Accepted for publication in the Astronomical Journal Feb. 200

    The scale of population structure in Arabidopsis thaliana

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    The population structure of an organism reflects its evolutionary history and influences its evolutionary trajectory. It constrains the combination of genetic diversity and reveals patterns of past gene flow. Understanding it is a prerequisite for detecting genomic regions under selection, predicting the effect of population disturbances, or modeling gene flow. This paper examines the detailed global population structure of Arabidopsis thaliana. Using a set of 5,707 plants collected from around the globe and genotyped at 149 SNPs, we show that while A. thaliana as a species self-fertilizes 97% of the time, there is considerable variation among local groups. This level of outcrossing greatly limits observed heterozygosity but is sufficient to generate considerable local haplotypic diversity. We also find that in its native Eurasian range A. thaliana exhibits continuous isolation by distance at every geographic scale without natural breaks corresponding to classical notions of populations. By contrast, in North America, where it exists as an exotic species, A. thaliana exhibits little or no population structure at a continental scale but local isolation by distance that extends hundreds of km. This suggests a pattern for the development of isolation by distance that can establish itself shortly after an organism fills a new habitat range. It also raises questions about the general applicability of many standard population genetics models. Any model based on discrete clusters of interchangeable individuals will be an uneasy fit to organisms like A. thaliana which exhibit continuous isolation by distance on many scales
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