9 research outputs found
Dropout and compliance to physical exercise in menopausal osteopenic women: the European “happy bones” project
IntroductionDecline in muscle mass and bone density seem to be two of the most disabling side effects of menopause that negatively affect women's quality of life. Promoting physical activity protocols in the workplace can represent a focal point in the prevention and management of several diseases. The study aims to evaluate the compliance and drop-out of menopausal osteopenic women engaged in combined training performed inside and outside the workplace. Strength and balance were analyzed to evaluate the effect of this protocol on osteoporosis prevention and the risk of falling.Methods73 menopausal women were enrolled in 5 European countries. They performed 72 lessons of a combined training proposed in the working place (IW) or sport center (SC).ResultsOut of the total 39 women enrolled in the IW, 12.8% had to leave the program, while out of the 34 women enrolled in SC, 41.2% did not complete the training. According to the compliance results, 47% of women that completed the trained IW and 85% in the SC recorded high compliance (p = 0.019). Moreover, the strength of the lower limbs (p < 0.001) and static balance (p = 0.001) significantly improved in the whole group.DiscussionIn conclusion, proposing well-structured training in the workplace for menopausal women seems to reduce drop-out. Strength and balance results suggest its positive impact on bone health and risk of falls, despite where it is performed
Foxa1 Knock-Out Via Crispr/Cas9 Altered Casp-9, Bax, Ccnd1, Cdk4, And Fibronectin Expressions In Lncap Cells
Prostate cancer is one of the most common types of cancer in men and the leading cause of death in developed countries. With the aid of molecular and genetic profiling of cancers, cancer molecular subtypes are paving the way for tailored cancer therapy. FOXA1 has been identified as one of the seven molecular subtypes of prostate cancer. FOXA1 is involved in a variety of metabolic process such as glucose homeostasis and deregulation of its expression is crucial in prostate cancer progression. In this study, we investigated the effects of FOXA1 gene knock-out on the expression levels of various cancer cell metabolism and cell cycle-related protein expressions. FOXA1 gene was knocked-out by using CRISPR/Cas9 technique. While FOXA1 gene knock-out significantly altered Casp-9, Bax, CCND1, CDK4, and fibronectin protein expressions (P < 0.05, fold change: similar to 40, 4.5, 2.5, 4.5, and 4, respectively), it did not affect the protein expression levels of Casp-3, Bcl-2, survivin, beta-catenin, c-Myc, and GSK-3B. Knocking-out FOXA1 gene in androgen-dependent LNCaP prostate cancer cells inhibited CCND1 protein expression. Our pre-clinical results demonstrate the importance of FOXA1 as a drug target in the treatment of prostate cancer. Impact statement Knock-out studies offer a unique way of studying the function of genes especially for developmentally lethal genes. FOXA1 has prominent roles both in breast and prostate cancer pathogenesis due to its role in ER receptor signaling pathway. FOXA1 has also been identified as one of the seven molecular subtypes of primary prostate cancer. In the present study, we used an efficient gene knock-out method, CRISPR/Cas9, in order to investigate FOXA1 function on LNCaP prostate cancer cells in vitro. FOXA1 knock-out altered cell-cycle regulator CCND1 protein expression levels. Therefore, our results suggest that FOXA1 might be a plausible drug target for prostate cancer treatment.Wo
FOXA1 knock-out via CRISPR/Cas9 altered Casp-9, Bax, CCND1, CDK4, and fibronectin expressions in LNCaP cells
Prostate cancer is one of the most common types of cancer in men and the
leading cause of death in developed countries. With the aid of molecular
and genetic profiling of cancers, cancer molecular subtypes are paving
the way for tailored cancer therapy. FOXA1 has been identified as one of
the seven molecular subtypes of prostate cancer. FOXA1 is involved in a
variety of metabolic process such as glucose homeostasis and
deregulation of its expression is crucial in prostate cancer
progression. In this study, we investigated the effects of FOXA1 gene
knock-out on the expression levels of various cancer cell metabolism and
cell cycle-related protein expressions. FOXA1 gene was knocked-out by
using CRISPR/Cas9 technique. While FOXA1 gene knock-out significantly
altered Casp-9, Bax, CCND1, CDK4, and fibronectin protein expressions (P
< 0.05, fold change: similar to 40, 4.5, 2.5, 4.5, and 4, respectively),
it did not affect the protein expression levels of Casp-3, Bcl-2,
survivin, beta-catenin, c-Myc, and GSK-3B. Knocking-out FOXA1 gene in
androgen-dependent LNCaP prostate cancer cells inhibited CCND1 protein
expression. Our pre-clinical results demonstrate the importance of FOXA1
as a drug target in the treatment of prostate cancer.
Impact statement
Knock-out studies offer a unique way of studying the function of genes
especially for developmentally lethal genes. FOXA1 has prominent roles
both in breast and prostate cancer pathogenesis due to its role in ER
receptor signaling pathway. FOXA1 has also been identified as one of the
seven molecular subtypes of primary prostate cancer. In the present
study, we used an efficient gene knock-out method, CRISPR/Cas9, in order
to investigate FOXA1 function on LNCaP prostate cancer cells in vitro.
FOXA1 knock-out altered cell-cycle regulator CCND1 protein expression
levels. Therefore, our results suggest that FOXA1 might be a plausible
drug target for prostate cancer treatment
Subnormal Growth Velocity and Related Factors During GnRH Analog Therapy for Idiopathic Central Precocious Puberty
Objective: Data concerning subnormal growth velocity (GV) and factors
that influence this during gonadotropin-releasing hormone analog (GnRHa)
therapy for idiopathic central precocious puberty (ICPP) are scarce. We
investigated the incidence of subnormal GV and associated factors in
patients receiving GnRHa therapy for ICPP.
Methods: In this retrospective cohort study, the records of 50 girls who
had been diagnosed with ICPP and started on GnRHa treatment before the
age of eight years were investigated. Subnormal GV frequency, related
factors during GnRHa therapy and the effect on final height were
examined.
Results: During the treatment, a significant decrease in the annual GV
and GV standard deviation score (SDS) of the patients was observed. In
16 (32\%) patients GV never declined below -1 SDS, while a decline was
noted once and twice in 19 (38\%) and 15 (30\%) patients respectively.
The median age of detection of subnormal GV was 9.9 (4.9-10.9) years.
Patients with pubic hair at diagnosis were found to have an increased
risk of subnormal GV (p = 0.016). There was a significant negative
correlation between diagnostic basal luteinizing hormone (LH) level and
the first and second year GV SDS (p = 0.012 and 0.017 respectively). A
significant negative correlation between bone age at diagnosis and 3rd
year GV SDS, and 4th year GV SDS (p = 0.002 and p = 0.038) was also
observed. LH suppression significantly increased during treatment (p =
0.001).
Conclusion: In girls with ICPP the risk of subnormal GV appears highest
at the 3rd year of GnRHa treatment, particularly in those patients with,
at the time of diagnosis, pubic hair in conjunction with high baseline
and peak LH and advanced BA and excessive LH suppression on follow-up
Comparative analysis of epi-miRNA expression levels in local/locally advanced and metastatic prostate cancer patients
Abnormal expression of enzymes involved in epigenetic mechanisms, such as DNA methyl transferases, can trigger large chaos in cellular gene expression networks and eventually lead to cancer progression. In our study, which is a pioneer in the literature that clinicopathologically evaluates the expression of 30 epi-miRNAs in prostate cancer (PCa), we investigated which of the new miRNA class epi-miRNAs could be an effective biomarker in the diagnosis and progression of PCa. In this study, the expression levels of 30 epi-miRNAs in whole blood samples from 25 control, 25 PCa and 40 metastatic PCa patients were investigated by the Quantitative Real-Time PCR method. Then, promoter methylation levels of 11 epi-miRNAs, whose expression levels were found to be significantly higher, were examined by methylation-specific qPCR method. The correlations between miRNA expression levels and clinicopathological parameters (Gleason Score (GS), PSA levels, TNM Staging) in different stages of PCa groups as well as disease-specific expression levels were examined. We found a hypomethylation in the promoter regions of miRNAs that showed a direct proportional increase with PSA levels (miR34b/c, miR-148a, miR-152), GS's (miR-34a-5p, miR-34b/c, miR-101-2, miR-126, miR-148a, miR-152, miR-1855p) and T staging (miR-34a-5p, miR-34b/c, miR-101-2, miR-126, miR-140, miR-148a, miR-152, miR-185-5p) (p < 0.05). When miR-200a/b was evaluated according to clinicopathological parameters, it acted as an oncomiR in local/local advanced PCa and as a tumor-suppressor-miR in metastatic stage. This study is novel in the sense that our findings draw attention to the important role of miRNAs as diagnostic and prognostic biomarkers in PCa.Gazi University Research FundGazi University [01/2019-11]This study was conducted with financial support from the Gazi University Research Fund [the project code number 01/2019-11].WOS:0005646940000012-s2.0-85088216243PubMed: 3268307
The effect of pneumoperitoneum on intravascular fibrinolytic activity in rats
Background/aim: Venous stasis during pneumoperitoneum in laparoscopic
surgery is closely related to fibrin synthesis and deposition. The
etiologic factors underlying fibrinolysis or hypercoagulability are not
clearly defined. This study aimed to determine the effects of
pneumoperitoneum time and pressure on coagulation cascade and the
fibrinolytic pathway.
Materials and methods: After the pneumoperitoneum model was established
in rats, PAI-1, tPA, TAFI, D-dimer, and fibrinogen activities were
evaluated in different time periods under different pressures in groups
including 6 rats. Group 1 did not undergo any procedure. Group 2
received 8 mmHg of pressure for 30 min, Group III 8 mmHg for 60 min,
Group IV 12 mmHg for 30 min, and Group V 12 mmHg for 60 min.
Results: D-dimer levels had a tendency to decrease with increasing
intraabdominal pressures. In both low and high pressure groups,
fibrinogen had a tendency to increase with exposure time. There was no
statistically significant difference among the study groups in terms of
fibrinogen, D-dimer, and PAI-1. The levels of TAFI were significantly
decreased with increasing pressure regardless of the exposure time.
Conclusion: Pneumoperitoneum of the coagulation system can be changed by
duration of time and pressure
Workplace physical violence, verbal violence, and mobbing experienced by nurses at a university hospital
Background/aim: The aim of this study was to determine the frequency of
and risk factors for physical violence, verbal violence, and mobbing
experienced by nurses in a university hospital.
Materials and methods: This was a cross-sectional study conducted at
Gazi University Medical Faculty Hospital. A questionnaire form
recommended by the WHO and the International Labor Organization was
administered through face-to-face interviews to determine the violence
experienced in the past 12 months by nurses.
Results: The prevalence of physical violence, verbal violence, and
mobbing was 13.9\%, 41.8\%, and 17.1\%, respectively. Working more than
40 h per week increased the risk of physical violence by 1.86 times. The
majority of nurses who experienced verbal violence and mobbing were
significantly more willing to change their work, their institution, and
their profession if given the opportunity. Fewer than one-fourth of the
victims indicated they reported any incident.
Conclusion: We knew that the prevalence of physical violence, verbal
violence, and mobbing were high among nurses and that incidents were
underreported, and the study corroborated this information. What this
study adds to the topic is that long working hours increased the
prevalence of physical violence and was defined as an important
contributory factor
Rotavirus infections in children in Turkey: A systematic review
We aimed to describe rotavirus epidemiology and clinical findings
including extraintestinal manifestations in a setting that has yet to
introduce rotavirus vaccines in the national immunization program. A
literature search was performed by using the key words ``Turkey{''} and
``rotavirus.{''} Ninety-eight studies published between 1987 and 2016
including epidemiological, clinical, and genotypical data at least 1
year duration were included. There were a total of 117 741 children with
diarrhea and 26 566 rotavirus gastroenteritis with a median detection
rate 31.8\% (95\% CI, 31.3-32.4) under 5 years of age. The rate of
dehydration was 47\% (95\% CI, 23.4-91.6). There were 328 cases reported
to be presenting with a various complication related to rotavirus in
2750 children in eight studies. The overall complication rate was 11.7\%
(95\% CI, 10.7-12.9). The cumulative incidence of the most common
genotypical combinations circulating worldwide was only 59.7\%
(G9{[}P8], 25\%; G1{[}P8], 22\%; G2{[}P4], 5.6\%; G3{[}P8], 2.6\%;
G4{[}P8], 4.5\%) whereas mixed, untypeable, and other genotypes were
2.4\%, 15\%, and 22.9\% respectively. Our results point out the
importance of rotavirus vaccination by presenting that rotavirus may
cause severe complications besides severe gastroenteritis. The role of
strain diversity in the variability of clinical presentations of
rotavirus infections needs to be further investigated