Psychomotricity and multiple intelligences

RESUMEN: En la actualidad, la sociedad ha sufrido y está sufriendo un gran cambio, el cual nos afecta a todos. Es por ello, que la educación tiene que atender a esto, renovando sus métodos de enseñanza, y actualizando los contenidos a trabajar. En este marco, surge el proyecto de investigación, titulado “Psicomotricidad e Inteligencias Múltiples”. Con él, se pretende profundizar en el extenso tema de las famosas Inteligencias Múltiples de Howard Gardner y su estrecha relación con la Psicomotricidad en la etapa de Educación Infantil. Asimismo, se trabajará concretamente con la inteligencia cinestésica-corporal y su vinculación con la psicomotricidad de forma teórica y práctica.ABSTRACT: Nowadays, the society is suffering a huge change that concern everyone. Therefore, the education system should take care of it and update its topics and teaching methods. Within all of that, this investigation project title “Psicomotricidad e inteligencias Múltiples” takes place. With this project, its intended firstly to take a deeper view inside the large topic that Howard Gardner called “multiples intelligences“ and secondly to see the close link between the “multiples intelligences” and the Psychomotricity during the children/infants education stage. Also, the kinesthetic intelligence and its connection with the theorical and practical psychomotricity will be specifically worked.Grado en Magisterio en Educación Infanti

Intratumor genetic heterogeneity and clonal evolution to decode endometrial cancer progression

Endometrial cancer; Clonal evolution; MutationCáncer endometrial; Evolución clonal; MutaciónCàncer d'endometri; Evolució clonal; MutacióAnalyzing different tumor regions by next generation sequencing allows the assessment of intratumor genetic heterogeneity (ITGH), a phenomenon that has been studied widely in some tumor types but has been less well explored in endometrial carcinoma (EC). In this study, we sought to characterize the spatial and temporal heterogeneity of 9 different ECs using whole-exome sequencing, and by performing targeted sequencing validation of the 42 primary tumor regions and 30 metastatic samples analyzed. In addition, copy number alterations of serous carcinomas were assessed by comparative genomic hybridization arrays. From the somatic mutations, identified by whole-exome sequencing, 532 were validated by targeted sequencing. Based on these data, the phylogenetic tree reconstructed for each case allowed us to establish the tumors’ evolution and correlate this to tumor progression, prognosis, and the presence of recurrent disease. Moreover, we studied the genetic landscape of an ambiguous EC and the molecular profile obtained was used to guide the selection of a potential personalized therapy for this patient, which was subsequently validated by preclinical testing in patient-derived xenograft models. Overall, our study reveals the impact of analyzing different tumor regions to decipher the ITGH in ECs, which could help make the best treatment decision.We thank all those at the Translational Research Laboratory of the MD Anderson Cancer Center Madrid for their invaluable help with this study. Tissue samples were obtained with the support of the MD Anderson Foundation Biobank (record number B.0000745, ISCIII National Biobank Record), the “Xarxa Catalana de Bancs de Tumors” and “Plataforma de Biobancos” ISCIII (PT13/0010/0014, B.000609). This study has been supported by the Spanish Ministry of Economy and Innovation (PID2019-104644RB-I00 (GMB), the Instituto de Salud Carlos III (ISCIII, CIBERONC, CB16/12/00295 - GMB-, CB16/12/00328 -EC, AGM- and CB16/12/00231 -XMG- [all partly supported by FEDER funds]) and by the AECC Scientific Foundation (FC_AECC PROYE19036MOR -GMB- and Coordinated groups 2018 -XMG, AGM, GMB-). SO is funded by an AECC-postdoctoral grant (2020). JSR-F and BW are funded in part by the Breast Cancer Research Foundation and in part by the NIH/NCI P50 CA247749 01 grant. Research reported in this publication was supported in part by a Cancer Center Support Grant of the NIH/NCI (Grant No. P30CA008748; MSK). We thank the Eurofins Megalab laboratory for helping us to perform the analysis of DNA HPV detection

Genomic profiling of uterine aspirates and cfDNA as an integrative liquid biopsy strategy in endometrial cancer

The incidence and mortality of endometrial cancer (EC) have risen in recent years, hence more precise management is needed. Therefore, wecombined di erent types of liquid biopsies to better characterize the genetic landscape of EC in a non-invasive and dynamic manner. Uterine aspirates (UAs) from 60 patients with EC were obtained during surgery and analyzed by next-generation sequencing (NGS). Blood samples, collected at surgery, were used for cell-free DNA (cfDNA) and circulating tumor cell (CTC) analyses. Finally, personalized therapies were tested in patient-derived xenografts (PDXs) generated from the UAs. NGS analyses revealed the presence of genetic alterations in 93% of the tumors. Circulating tumor DNA (ctDNA) was present in 41.2% of cases, mainly in patients with high-risk tumors, thus indicating a clear association with a more aggressive disease. Accordingly, the results obtained during the post-surgery follow-up indicated the presence of ctDNA in three patients with progressive disease. Moreover, 38.9% of patients were positive for CTCs at surgery. Finally, the e cacy of targeted therapies based on the UA-specific mutational landscape was demonstrated in PDX models. Our study indicates the potential clinical applicability of a personalized strategy based on a combination of different liquid biopsies to characterize and monitor tumor evolution, and to identify targeted therapiesThis work was supported by grants and support from the Instituto de Salud Carlos III (ISCIII) and FEDER (PI17/01919, PI17/02071), CIBERONC (CB16/12/00328), and the AECC (Grupos Estables de Investigacion 2018-AECC) to A.G.-M. and M.A.; Instituto de Salud Carlos III (ISCIII) and FEDER (PI16/00134), CIBERONC (CB16/12/00295), and the AECC (Grupos Estables de Investigacion 2018-AECC) to G.M.-B.; and the AECC to L.M.-R

Perinatal outcomes in pregnancies after oocyte donation in young women

RESUMEN : Introducción: Estudios previos han demostrado un mayor riesgo de complicaciones obstétricas y perinatales en gestaciones obtenidas mediante ovodonación. Objetivo: Evaluar el impacto de la ovodonación sobre los resultados obstétricos y perinatales, a través del análisis de dos parámetros: parto pretérmino y patología relacionada con el aumento de la resistencia de las arterias uterinas. Material y métodos: Se trata de un estudio de cohortes retrospectivo en el que se incluyeron mujeres con parto en el Hospital Universitario Marqués de Valdecilla (HUMV) entre enero de 2014 y diciembre de 2019. Estas mujeres se dividieron en tres grupos ; el primero compuesto por mujeres ≤ 38 años incluidas dentro del programa de ovodonación de la Unidad de Reproducción Asistida del HUMV, el segundo mujeres que concibieron tras un tratamiento de FIV-ICSI con óvulos autólogos y el tercero es un grupo control con gestantes por concepción espontánea. Resultados: El grupo de gestantes mediante ovodonación presentó un mayor riesgo de patología relacionada con el aumento de la resistencia de las arterias uterinas en comparación con el grupo de FIV-óvulos autólogos (RR=3’579 IC95%: 1’076-11’906) y con el grupo de gestaciones espontáneas (RR=3’841 IC95%: 1’152-12’812). No se ha encontrado una relación estadísticamente significativa entre el uso de óvulos donados y la incidencia de parto pretérmino, si bien los resultados parecen indicarnos una tendencia hacia un mayor riesgo de esta complicación con esta técnica. Conclusiones: La ovodonación se relaciona con un mayor riesgo de ciertas complicaciones obstétricas y perinatales en comparación con las gestaciones conseguidas tras FIV con óvulos autólogos y con las gestaciones espontáneas. La razón de esta asociación se desconoce, pero muy probablemente una de las causas sea inmunológica.ABSTRACT : Introduction: Previous studies have demonstrated a higher risk of maternal and perinatal complications in pregnancies after oocyte donation. Objective: Assess the impact of oocyte donation on obstetric and perinatal outcomes, through the analysis of two parameters: preterm birth and pathology associated with increased resistance to blood flow in the uterine arteries. Material and methods: This is a retrospective cohort study that includes women that gave birth in Hospital Universitario Marqués de Valdecilla (HUMV) from January 2014 to December 2019. These women were divided into three different groups: the first one is composed of women ≤38 years old included in the egg donation program of the assisted reproduction unit of HUMV, the second one of women that conceived after a IVF-ICSI treatment using autologous oocytes and the third one is a control group made up of women that conceived spontaneously. Results: The oocyte donation pregnancies group showed a greater risk of pathology associated with increased resistance to blood flow in the uterine arteries compared with the IVF-autologous oocytes group (RR=3’579 IC95%: 1’076-11’906) and with the spontaneous conception group (RR=3’841 IC95%: 1’152-12’812). No statistically significant relationship was observed between the use of donated oocytes and incidence of preterm birth, nonetheless the results seem to indicate a trend towards an increased risk of this complication when using the aforesaid technique. Conclusions: Oocyte donation is associated with an increased risk of certain obstetric and perinatal complications compared with pregnancies using IVF-autologous oocytes and with spontaneously conceived pregnancies. The reason behind this relationship is yet unknown, but one of the causes is probably immunologic.Grado en Medicin

Electrical Continuity Testing of Through-Silicon Vias Under Static Force Application

Through-silicon via (TSV) is a high performance interconnect technology and a new facet of advanced memory packaging which seeks to vastly improve memory density and efficiency. It employs the use of conductive vertical pillars passing through and connecting all of the stacked layers of silicon to the memory substrate. Since it is an early and contemporary technology, more information is needed about electrical and mechanical reliabilities of TSVs under conditions of environmental stress. To this end, we are developing a testing device which will provide real-time data regarding the electrical continuity of approximately 400 TSV interconnects on a prototype memory package as they are subjected to static forces. This will help facilitate identification of when and where a given interconnect failure occurs while providing comprehensive continuity data from the test. Moving forward, this data will assist in the understanding of failure causes and prove useful in adapting TSV design and implementation

Intratumor genetic heterogeneity and clonal evolution to decode endometrial cancer progression

Analyzing different tumor regions by next generation sequencing allows the assessment of intratumor genetic heterogeneity (ITGH), a phenomenon that has been studied widely in some tumor types but has been less well explored in endometrial carcinoma (EC). In this study, we sought to characterize the spatial and temporal heterogeneity of 9 different ECs using whole-exome sequencing, and by performing targeted sequencing validation of the 42 primary tumor regions and 30 metastatic samples analyzed. In addition, copy number alterations of serous carcinomas were assessed by comparative genomic hybridization arrays. From the somatic mutations, identified by whole-exome sequencing, 532 were validated by targeted sequencing. Based on these data, the phylogenetic tree reconstructed for each case allowed us to establish the tumors' evolution and correlate this to tumor progression, prognosis, and the presence of recurrent disease. Moreover, we studied the genetic landscape of an ambiguous EC and the molecular profile obtained was used to guide the selection of a potential personalized therapy for this patient, which was subsequently validated by preclinical testing in patient-derived xenograft models. Overall, our study reveals the impact of analyzing different tumor regions to decipher the ITGH in ECs, which could help make the best treatment decisio