Exploring the links between urban agriculture, land use and food security in the Philippi Horticultural Area (PHA)

Magister Artium - MAHunger is more than just a feeling, it is the lack of access to safe nutritious food, which in turn may result in anger towards government, low performance, sadness and a limited will to survive. Urban agriculture has been identified as a source of livelihood for many urban residents and could fundamentally change food insecure cities like Cape Town. The Philippi Horticultural Area (PHA) is one such place with an enormous amount of potential to assist the City of Cape Town (CCT) to overcome food insecurity challenges. The PHA is the focus of this thesis that aims to determine the impacts that housing and industrial developments in the PHA have had, and might have in the future, on food security in the Greater Cape Town Area (GCTA). The specific objectives of the study are as follows: (1) To investigate the urban agricultural distribution of the PHA; (2) to investigate agricultural facilitation, people empowerment and the use of land for agricultural purposes; (3) To determine the level of access to food for people within and around the PHA; and (4) To examine the links between the urban agricultural food sector and food production. Mixed method research was employed, hinging on the Sustainable Livelihoods Approach (SLA) as the conceptual framework. Snowball sampling was used to select 68 participants who were interviewed. One key finding of the study showed that the PHA had a significant value to the participants, many of whom called the place ‘home’. Another finding is that urban agriculture provides fresh food produce to many local residents. In-depth discussions with officials and farmers, both commercial and small-scale farmers in the PHA, revealed that the PHA is a valuable portion of farmland, and contributes significantly towards food security in and around the PHA. With the use of the SLA as the conceptual framework, the study contributes towards other livelihood outcomes dependant on urban agriculture to improve access, availability and stability of food security within the PHA. Although urban agriculture is a minimal contributor to food security in the PHA, there are other benefits enjoyed by low-income communities such as food aid given by farmers to assist low-income housing communities, educational opportunities to enhance small growers in the PHA, small-scale community garden outreach and employment

Strategies for Turning Ideas into Business at Universities

Universities are well known for undertaking research projects in various sectors and making technological breakthroughs but few universities have focused on actually turning ideas into businesses. More and more universities are beginning to realize that it is no longer adequate to simply produce graduates and doctoral theses. They must play a greater role in generating wealth for their country and contributing to society by ensuring that promising inventions and other creative ideas do not get lost but be developed into marketable products and services. As a result, there is currently a growing trend whereby universities are taking steps to become more entrepreneurial by encouraging students and staff to generate creative ideas and commercialize their ideas. This paper examines the programmes of Universities at the International Level that have been promoting entrepreneurship development and recommends intervention areas to increase the capacity of a University to derive the benefits from an active business commercialization process. The key recommendations include the development of an entrepreneurial vision for the University, cultivating partnerships with private and public sector organizations and   creating linkages that will promote mentorship and funding opportunities.  Keywords: Entrepreneurial University, Business Commercialization, Innovation, Triple Helix Mode

The breadbasket of Cape Town: Exploring the links between urban agriculture, land use and food security in the Philippi Horticultural Area (PHA)

Magister Artium - MAHunger is more than just a feeling, it is the lack of access to safe nutritious food, which in turn may result in anger towards government, low performance, sadness and a limited will to survive. Urban agriculture has been identified as a source of livelihood for many urban residents and could fundamentally change food insecure cities like Cape Town. The Philippi Horticultural Area (PHA) is one such place with an enormous amount of potential to assist the City of Cape Town (CCT) to overcome food insecurity challenges. The PHA is the focus of this thesis that aims to determine the impacts that housing and industrial developments in the PHA have had, and might have in the future, on food security in the Greater Cape Town Area (GCTA). The specific objectives of the study are as follows: (1) To investigate the urban agricultural distribution of the PHA; (2) to investigate agricultural facilitation, people empowerment and the use of land for agricultural purposes; (3) To determine the level of access to food for people within and around the PHA; and (4) To examine the links between the urban agricultural food sector and food production

Development and Validation of the Geriatric Anxiety Inventory

Background: Anxiety symptoms and anxiety disorders are highly prevalent among elderly people, although infrequently the subject of systematic research in this age group. One important limitation is the lack of a widely accepted instrument to measure dimensional anxiety in both normal old people and old people with mental health problems seen in various settings. Accordingly, we developed and tested of a short scale to measure anxiety in older people. Methods:We generated a large number of potential items de novo and by reference to existing anxiety scales, and then reduced the number of items to 60 through consultation with a reference group consisting of psychologists, psychiatrists and normal elderly people. We then tested the psychometric properties of these 60 items in 452 normal old people and 46 patients attending a psychogeriatric service. We were able to reduce the number of items to 20. We chose a 1-week perspective and a dichotomous response scale. Results: Cronbach's alpha for the 20-item Geriatric Anxiety Inventory (GAI) was 0.91 among normal elderly people and 0.93 in the psychogeriatric sample. Concurrent validity with a variety of other measures was demonstrated in both the normal sample and the psychogeriatric sample. Inter-rater and test-retest reliability were found to be excellent. Receiver operating characteristic analysis indicated a cut-point of 10/11 for the detection of DSM-IV Generalized Anxiety Disorder (GAD) in the psychogeriatric sample, with 83% of patients correctly classified with a specificity of 84% and a sensitivity of 75%. Conclusions: The GAI is a new 20-item self-report or nurse-administered scale that measures dimensional anxiety in elderly people. It has sound psychometric properties. Initial clinical testing indicates that it is able to discriminate between those with and without any anxiety disorder and between those with and without DSM-IV GAD

Novel Pharmacologic Targeting of Tight Junctions and Focal Adhesions in Prostate Cancer Cells

Cancer cell resistance to anoikis driven by aberrant signaling sustained by the tumor microenvironment confers high invasive potential and therapeutic resistance. We recently generated a novel lead quinazoline-based Doxazosin® derivative, DZ-50, which impairs tumor growth and metastasis via anoikis. Genome-wide analysis in the human prostate cancer cell line DU-145 identified primary downregulated targets of DZ-50, including genes involved in focal adhesion integrity (fibronectin, integrin-α6 and talin), tight junction formation (claudin-11) as well as insulin growth factor binding protein 3 (IGFBP-3) and the angiogenesis modulator thrombospondin 1 (TSP-1). Confocal microscopy demonstrated structural disruption of both focal adhesions and tight junctions by the downregulation of these gene targets, resulting in decreased cell survival, migration and adhesion to extracellular matrix (ECM) components in two androgen-independent human prostate cancer cell lines, PC-3 and DU-145. Stabilization of cell-ECM interactions by overexpression of talin-1 and/or exposing cells to a fibronectin-rich environment mitigated the effect of DZ-50. Loss of expression of the intracellular focal adhesion signaling effectors talin-1 and integrin linked kinase (ILK) sensitized human prostate cancer to anoikis. Our findings suggest that DZ-50 exerts its antitumor effect by targeting the key functional intercellular interactions, focal adhesions and tight junctions, supporting the therapeutic significance of this agent for the treatment of advanced prostate cancer

A comparison of sequencing platforms and bioinformatics pipelines for compositional analysis of the gut microbiome

Abstract Background Advancements in Next Generation Sequencing (NGS) technologies regarding throughput, read length and accuracy had a major impact on microbiome research by significantly improving 16S rRNA amplicon sequencing. As rapid improvements in sequencing platforms and new data analysis pipelines are introduced, it is essential to evaluate their capabilities in specific applications. The aim of this study was to assess whether the same project-specific biological conclusions regarding microbiome composition could be reached using different sequencing platforms and bioinformatics pipelines. Results Chicken cecum microbiome was analyzed by 16S rRNA amplicon sequencing using Illumina MiSeq, Ion Torrent PGM, and Roche 454 GS FLX Titanium platforms, with standard and modified protocols for library preparation. We labeled the bioinformatics pipelines included in our analysis QIIME1 and QIIME2 (de novo OTU picking [not to be confused with QIIME version 2 commonly referred to as QIIME2]), QIIME3 and QIIME4 (open reference OTU picking), UPARSE1 and UPARSE2 (each pair differs only in the use of chimera depletion methods), and DADA2 (for Illumina data only). GS FLX+ yielded the longest reads and highest quality scores, while MiSeq generated the largest number of reads after quality filtering. Declines in quality scores were observed starting at bases 150–199 for GS FLX+ and bases 90–99 for MiSeq. Scores were stable for PGM-generated data. Overall microbiome compositional profiles were comparable between platforms; however, average relative abundance of specific taxa varied depending on sequencing platform, library preparation method, and bioinformatics analysis. Specifically, QIIME with de novo OTU picking yielded the highest number of unique species and alpha diversity was reduced with UPARSE and DADA2 compared to QIIME. Conclusions The three platforms compared in this study were capable of discriminating samples by treatment, despite differences in diversity and abundance, leading to similar biological conclusions. Our results demonstrate that while there were differences in depth of coverage and phylogenetic diversity, all workflows revealed comparable treatment effects on microbial diversity. To increase reproducibility and reliability and to retain consistency between similar studies, it is important to consider the impact on data quality and relative abundance of taxa when selecting NGS platforms and analysis tools for microbiome studies

Genetic Interaction Analysis Among Oncogenesis-Related Genes Revealed Novel Genes and Networks in Lung Cancer Development

The development of cancer is driven by the accumulation of many oncogenesis-related genetic alterationsand tumorigenesis is triggered by complex networks of involved genes rather than independent actions. To explore the epistasis existing among oncogenesis-related genes in lung cancer development, we conducted pairwise genetic interaction analyses among 35,031 SNPs from 2027 oncogenesis-related genes. The genotypes from three independent genome-wide association studies including a total of 24,037 lung cancer patients and 20,401 healthy controls with Caucasian ancestry were analyzed in the study. Using a two-stage study design including discovery and replication studies, and stringent Bonferroni correction for multiple statistical analysis, we identified significant genetic interactions between SNPs in RGL1:RAD51B (OR=0.44, p value=3.27x10-11 in overall lung cancer and OR=0.41, p value=9.71x10-11 in non-small cell lung cancer), SYNE1:RNF43 (OR=0.73, p value=1.01x10-12 in adenocarcinoma) and FHIT:TSPAN8 (OR=1.82, p value=7.62x10-11 in squamous cell carcinoma) in our analysis. None of these genes have been identified from previous main effect association studies in lung cancer. Further eQTL gene expression analysis in lung tissues provided information supporting the functional role of the identified epistasis in lung tumorigenesis. Gene set enrichment analysis revealed potential pathways and gene networks underlying molecular mechanisms in overall lung cancer as well as histology subtypes development. Our results provide evidence that genetic interactions between oncogenesis-related genes play an important role in lung tumorigenesis and epistasis analysis, combined with functional annotation, provides a valuable tool for uncovering functional novel susceptibility genes that contribute to lung cancer development by interacting with other modifier genes

Fibronectin acts as a molecular switch to determine SPARC function in pancreatic cancer

Pancreatic ductal adenocarcinoma (PDAC) is one of the deadliest of all solid tumours and more effective therapy is urgently needed. The stroma is thought to play a critical role in tumour development and metastasis, and high stromal expression of the matricellular protein SPARC has been robustly associated with poor patient prognosis. However, the precise role of SPARC has been highly controversial, with multiple studies demonstrating tumour-suppressor properties of this protein 'in vitro'. This conflicting data has been a barrier to the development of new therapeutic approaches targeting SPARC, despite current interest in stromal-therapy. We show conclusively that SPARC acts directly on cancer cells to promote pancreatic cancer cell proliferation. This contradicts previous 'in vitro' studies, but is consistent with the observed clinical association between SPARC expression and poor patient prognosis. However, depletion of fibronectin switches the activity of SPARC from promoting cancer cell proliferation to growth inhibition and induction of apoptosis. Thus, targeting the interaction between SPARC and fibronectin could be used to turn the highly expressed tumour protein SPARC against the tumour to induce tumour cytotoxicity, and is a novel target for PDAC therapy

UK consensus guidelines for the delivery of unexpected news in obstetric ultrasound: The ASCKS framework

Background: Studies indicate there is a need to improve the delivery of unexpected news via obstetric ultrasound, but there have been few advances in this area. One factor preventing improvement has been a lack of consensus regarding the appropriate phrases and behaviours which sonographers and ultrasound practitioners should use in these situations. Aims: To develop consensus guidelines for unexpected news delivery in Early Pregnancy Unit and Fetal Anomaly Screening Programme NHS settings. Methods: A workshop was conducted to identify priorities and reach consensus on areas of contention. Contributors included interdisciplinary healthcare professionals, policy experts, representatives from third-sector organisations, lay experts and academic researchers (n = 28). Written and verbal feedback was used to draft initial guidance which was then circulated amongst the wider writing group (n = 39). Revisions were undertaken until consensus was reached. Results: Consensus guidelines were developed outlining the behaviours and phrases which should be used during scans where unexpected findings are identified. Specific recommendations included that: honest and clear communication should be prioritised, even with uncertain findings; technical terms should be used, but these should be written down together with their lay interpretations; unless expectant parents use other terminology (e.g. ‘foetus’), the term ‘baby’ should be used as a default, even in early pregnancy; at the initial news disclosure, communication should focus on information provision. Expectant parents should not be asked to make decisions during the scan. Conclusions: These recommendations can be used to develop and improve news delivery interventions in obstetric ultrasound settings. The full guidelines can be accessed online as supplemental material and at https://doi.org/10.5518/100/24