Contributions of point extragalactic sources to the Cosmic Microwave Background bispectrum

All the analyses of Cosmic Microwave Background (CMB) temperature maps up--to--date show that CMB anisotropies follow a Gaussian distribution. On the other hand, astrophysical foregrounds which hamper the detection of the CMB angular power spectrum, are not Gaussian distributed on the sky. Therefore, they should give a sizeable contribution to the CMB bispectrum. In fact, the first year data of the Wilkinson Microwave Anisotropy Probe (WMAP) mission have allowed the {\it first} detection of the extragalactic source contribution to the CMB bispectrum at 41 GHz and, at the same time, much tighter limits than before to non--Gaussian primordial fluctuations. In view of the above and for achieving higher precision in current and future CMB measurements of non--Gaussianity, in this paper we discuss a comprehensive assessment of the bispectrum due to either uncorrelated and clustered extragalactic point sources in the whole frequency interval around the CMB intensity peak. Our calculations, based on current cosmological evolution models for sources, show that the reduced angular bispectrum due to point sources, $b_{ps}$, should be detectable in all WMAP and Planck frequency channels. We also find agreement with the results on $b_{ps}$ at 41 GHz coming from the analysis of the first year WMAP data. Moreover, by comparing $b_{ps}$ with the primordial reduced CMB bispectrum, we find that only the peak value of the primordial bispectrum (which appears at $l\simeq 200$) results greater than $b_{ps}$ in a frequency window around the intensity peak of the CMB. The amplitude of this window basically depends on the capability of the source detection algorithms (i.e., on the achievable flux detection limit, $S_{lim}$, for sources).Comment: 26 pages, 6 Figures, use AasTex5.0, ApJ, in press, Oct. 10, 2003 Issu

Non-blind catalogue of extragalactic point sources from the Wilkinson Microwave Anisotropy Probe (WMAP) first 3--year survey data

We have used the MHW2 filter to obtain estimates of the flux densities at the WMAP frequencies of a complete sample of 2491 sources, mostly brighter than 500 mJy at 5 GHz, distributed over the whole sky excluding a strip around the Galactic equator (b < 5 degrees). After having detected 933 sources above the 3 sigma level in the MHW2 filtered maps - our New Extragalactic WMAP Point Source (NEWPS_3sigma) Catalogue - we are left with 381 sources above 5 sigma in at least one WMAP channel, 369 of which constitute our NEWPS_5sigma catalogue. It is remarkable to note that 98 (i.e. 26%) sources detected above 5 sigma are `new', they are not present in the WMAP catalogue. Source fluxes have been corrected for the Eddington bias. Our flux density estimates before such correction are generally in good agreement with the WMAP ones at 23 GHz. At higher frequencies WMAP fluxes tend to be slightly higher than ours, probably because WMAP estimates neglect the deviations of the point spread function from a Gaussian shape. On the whole, above the estimated completeness limit of 1.1 Jy at 23 GHz we detected 43 sources missed by the blind method adopted by the WMAP team. On the other hand, our low-frequency selection threshold left out 25 WMAP sources, only 12 of which, however, are 5 sigma detections and only 3 have fluxes S at 23 GHz > 1.1 Jy. Thus, our approach proved to be competitive with, and complementary to the WMAP one.Comment: 18 pages, 6 figures, 5 tables. Accepted for publication in ApJ

Isotropic Wavelets: a Powerful Tool to Extract Point Sources from CMB Maps

It is the aim of this paper to introduce the use of isotropic wavelets to detect and determine the flux of point sources appearing in CMB maps. The most suited wavelet to detect point sources filtered with a Gaussian beam is the Mexican Hat. An analytical expression of the wavelet coefficient obtained in the presence of a point source is provided and used in the detection and flux estimation methods presented. For illustration the method is applied to two simulations (assuming Planck Mission characteristics) dominated by CMB (100 GHz) and dust (857 GHz) as these will be the two signals dominating at low and high frequency respectively in the Planck channels. We are able to detect bright sources above 1.58 Jy at 857 GHz (82% of all sources) and above 0.36 Jy at 100 GHz (100% of all) with errors in the flux estimation below 25%. The main advantage of this method is that nothing has to be assumed about the underlying field, i.e. about the nature and properties of the signal plus noise present in the maps. This is not the case in the detection method presented by Tegmark and Oliveira-Costa 1998. Both methods are compared producing similar results.Comment: 6 pages. Accepted for publication in MNRA

Forecasts on the contamination induced by unresolved point sources in primordial non-Gaussianity beyond Planck

In this paper we present forecasts of the contamination on different shapes of the primordial non-Gaussianity fnl parameter \u2013 detectable on future Cosmic Microwave Background (CMB) high\u2013resolution anisotropy maps \u2013 produced by unresolved ex- tragalactic point sources at frequencies of cosmological interest (45\u2013375 GHz). We consider two scenarios: an ideal (noiseless) mission and a possible future space-borne satellite, with instrumental characteristics similar to the ones proposed for the Cos- mic Origins Explorer (COrE). The local, equilateral, orthogonal and flat shapes are considered in both temperature (intensity) and polarized emission data. The angular power spectrum and bispectrum of extragalactic point sources are estimated by state- of-the-art models of source number counts. The impact of all the most relevant (far\u2013IR and radio selected) source populations on these shapes at COrE frequencies is studied. The results of this analysis show that unresolved extragalactic point sources should not induce a very relevant non-Gaussian signal in the frequency range 100\u2013200GHz, thus not preventing a correct estimate of the CMB primordial fnl parameter. Polariza- tion information allows one to significantly reduce the error\u2013bars in the fnl parameter and the bias induced by unresolved sources and, hence, to widen the range of frequen- cies for fnl studies. On the contrary, at \u3bd 225GHz, important non-Gaussian deviations in CMB anisotropy maps are expected due to unresolved extragalactic sources

Spherical Mexican Hat wavelet: an application to detect non-Gaussianity in the COBE-DMR maps

The spherical Mexican Hat wavelet is introduced in this paper, with the aim of testing the Gaussianity of the Cosmic Microwave Background temperature fluctuations. Using the information given by the wavelet coefficients at several scales, we have performed several statistical tests on the COBE-DMR maps to search for evidence of non-Gaussianity. Skewness, kurtosis, scale-scale correlations (for two and three scales) and Kolmogorov-Smirnov tests indicate that the COBE-DMR data are consistent with a Gaussian distribution. We have extended the analysis to compare temperature values provided by COBE-DMR data with distributions (obtained from Gaussian simulations) at each pixel and at each scale. The number of pixels with temperature values outside the 95% and the 99% is consistent with that obtained from Gaussian simulations, at all scales. Moreover, the extrema values for COBE-DMR data (maximum and minimum temperatures in the map) are also consistent with those obtained from Gaussian simulations.Comment: to be published in MNRA

Programmed cell death induced by high levels of cytokinin in Arabidopsis cultured cells is mediated by the cytokinin receptor CRE1/AHK4

High levels of cytokinins (CKs) induce programmed cell death (PCD) both in animals and plant cells. High levels of the CK benzylaminopurine (BA) induce PCD in cultured cells of Arabidopsis thaliana by accelerating a senescence process characterized by DNA laddering and expression of a specific senescence marker. In this report, the question has been addressed whether members of the small family of Arabidopsis CK receptors (AHK2, AHK3, CRE1/AHK4) are required for BA-induced PCD. In this respect, suspension cell cultures were produced from selected receptor mutants. Cell growth and proliferation of all receptor mutant and wild-type cell cultures were similar, showing that the CK receptors are not required for these processes in cultured cells. The analysis of CK metabolites instead revealed differences between wild-type and receptor mutant lines, and indicated that all three receptors are redundantly involved in the regulation of the steady-state levels of isopentenyladenine- and trans-zeatin-type CKs. By contrast, the levels of cis-zeatin-type CKs were controlled mainly by AHK2 and AHK3. To study the role of CK receptors in the BA-induced PCD pathway, cultured cells were analysed for their behaviour in the presence of high levels of BA. The results show that CRE1/AHK4, the strongest expressed CK receptor gene of this family in cultured cells, is required for PCD, thus linking this process to the known CK signalling pathway

Establishing the tolerability and performance of tamarind seed polysaccharide (TSP) in treating dry eye syndrome: results of a clinical study

BACKGROUND: One of the problems arising from available preparations for dry eye syndrome is the limited residence time of products on the ocular surface. In this paper, we look at an innovative new treatment for dry eye, tamarind seed polysaccharide (TSP). TSP possesses mucomimetic, mucoadhesive and pseudoplastic properties. The 'mucin-like' molecular structure of TSP is similar to corneal and conjunctival mucin 1 (MUC1), a transmembrane glycoprotein thought to play an essential role in protecting and wetting the corneal surface and may explain its increased retention on the eye surface. METHODS: The activity of TSP and hyaluronic acid (HA) in the treatment of dry eye syndrome was compared in an open-label, randomised, single-centre clinical study. Thirty patients were randomised to receive three or more applications per day of either TSP 0.5%, TSP 1% or HA 0.2% (Hyalistil™) over a period of 90 days. The primary objective of tolerability was assessed by visual analogue scale (VAS), scoring of specific symptoms and the incidence of adverse events. Secondary objectives included improvement in stability of the precorneal tear film, subjective symptoms and corneal and conjunctival staining. RESULTS: TSP 0.5% and 1% were comparable to HA 0.2% with regard to both primary and secondary objective parameters. TSP 1% showed benefits over HA 0.2% for the subjective symptoms; trouble blinking, ocular burning and foreign body sensation. CONCLUSION: This study suggests that TSP 0.5% and 1% offer at least equivalent relief to HA 0.2% for dry eye syndrome. All treatments demonstrated optimal tolerability and are suitable for frequent use in the therapy of dry eye. TSP 1% produced promising results in terms of improvements in certain patient symptoms and suggests benefits of the TSP formulation. This study paves the way for a larger study to further establish the performance and safety of TSP compared with HA and highlights the need to expand this therapeutic agent to a wider dry eye population

The Baker's Yeast Diploid Genome Is Remarkably Stable in Vegetative Growth and Meiosis

Accurate estimates of mutation rates provide critical information to analyze genome evolution and organism fitness. We used whole-genome DNA sequencing, pulse-field gel electrophoresis, and comparative genome hybridization to determine mutation rates in diploid vegetative and meiotic mutation accumulation lines of Saccharomyces cerevisiae. The vegetative lines underwent only mitotic divisions while the meiotic lines underwent a meiotic cycle every ∼20 vegetative divisions. Similar base substitution rates were estimated for both lines. Given our experimental design, these measures indicated that the meiotic mutation rate is within the range of being equal to zero to being 55-fold higher than the vegetative rate. Mutations detected in vegetative lines were all heterozygous while those in meiotic lines were homozygous. A quantitative analysis of intra-tetrad mating events in the meiotic lines showed that inter-spore mating is primarily responsible for rapidly fixing mutations to homozygosity as well as for removing mutations. We did not observe 1–2 nt insertion/deletion (in-del) mutations in any of the sequenced lines and only one structural variant in a non-telomeric location was found. However, a large number of structural variations in subtelomeric sequences were seen in both vegetative and meiotic lines that did not affect viability. Our results indicate that the diploid yeast nuclear genome is remarkably stable during the vegetative and meiotic cell cycles and support the hypothesis that peripheral regions of chromosomes are more dynamic than gene-rich central sections where structural rearrangements could be deleterious. This work also provides an improved estimate for the mutational load carried by diploid organisms

High serum levels of tissue inhibitor of matrix metalloproteinase-1 during the first week of a malignant middle cerebral artery infarction in non-surviving patients

Background: Higher circulating levels of tissue inhibitor of matrix metalloproteinases (TIMP)-1 early after ischemic stroke have been associated with lower survival. The objectives of this study were to determine serum TIMP-1 levels during the first week of a severe cerebral infarction in surviving and non-surviving patients, and whether those levels during the first week could be used as a mortality biomarker for these patients. Methods: We included patients with severe malignant middle cerebral artery infarction (MMCAI) defined as computer tomography showing ischaemic changes in more than 50% of the middle cerebral artery territory and Glasgow Coma Scale (GCS) ≤ 8. We measured serum levels of matrix metalloproteinases (MMP)-9 and TIMP-1. End-point study was 30-day mortality. Results: We found higher TIMP-1 concentrations at days 1 (p < 0.001), 4 (p = 0.001), and 8 (p = 0.03) of MMCAI in nonurviving (n = 34) than in surviving (n = 34) patients. We found lower serum MMP-9 concentrations at day 1 (p = 0.03) of MMCAI and no significant differences at days 4 and 8. ROC curve analysis of TIMP-1 concentrations performed at days 1, 4, and 8 of MMCAI showed an area under curve to predict 30-day mortality of 81% (p < 0.001), 80% (p < 0.001) and 72% (p = 0.07) respectively. Conclusions: The new findings of our study were that non-surviving MMCAI patients showed higher serum TIMP-1 levels during the first week of MMCAI that surviving patients, and those levels during the first week of MMCAI could be used as mortality biomarkers