Salmonelosis porcina en España: factores de riesgo en reproductores, estrategias de control en cerdos de cebo y la importancia del sacrificio = Salmonella infection in pig production in Spain: risk factors in breeding pigs, control strategies in finishing pigs and the role of the slaughtering in the pork contamination

306 p.El objetivo general de la presente tesis se ha dirigido hacia el incremento del conocimiento relativo a la epidemiología y el control de la infección por Salmonella en el ganado porcino. Los estudios realizados se desarrollan en tres capítulos que abarcan las explotaciones de reproductores, el control de la infección en las granjas de cebo y las etapas posteriores a la granja: transporte, espera y sacrificio. En total, la tesis incluye seis publicaciones científicas originales y dos revisiones, una publicada como artículo de revisión en una revista científica y la otra incluida como capítulo de un libr

Requerimientos para la implementación y obtención de certificación HACCP en la planta de alimentos balanceados de concentrados "El Granjero" S.A., durante el período del 27 de Septiembre del 2006 al 20 de Abril del 2007

El sistema HACCP fue desarrollado inicialmente para controlar la seguridad microbiológica de los alimentos para los astronautas del Programa Espacial Tripulado de los EEUU, dado que era vital garantizar que estos fueran seguros. En esa época los sistemas de aseguramiento de Calidad estaban basados en el análisis del producto final, pero se comprobó que analizando el 100% de los productos, se podría garantizar su seguridad

Taller vertical de arquitectura 09

Trabajos presentados por los alumnos en los diferentes talleres> de la FAU entre los años 2003 y 2005. Selección de ejercicios de los niveles 1 a 6 de la cátedra Krause-Tomas-Argüello, sobre los temas:.bar en el río, tres viviendas, municipalidad en ciudad pequeña, club social deportivo y cultural, clínica con internación y nuevos equipamientos y servicios metropolitanos en Dársena Sur.Facultad de Arquitectura y Urbanism

Taller vertical de arquitectura 09

Trabajos presentados por los alumnos en los diferentes talleres> de la FAU entre los años 2003 y 2005. Selección de ejercicios de los niveles 1 a 6 de la cátedra Krause-Tomas-Argüello, sobre los temas:.bar en el río, tres viviendas, municipalidad en ciudad pequeña, club social deportivo y cultural, clínica con internación y nuevos equipamientos y servicios metropolitanos en Dársena Sur.Facultad de Arquitectura y Urbanism

Characterization of Antimicrobial Resistance Determinants and Class 1 and Class 2 Integrons in Salmonella enterica spp., Multidrug-Resistant Isolates from Pigs

[EN] Antimicrobial resistance (AMR) and Salmonella spp., are primary concerns in public health. The present study characterizes the AMR determinants of 62 multi-drug resistant (MDR) Salmonella enterica spp., isolates from swine, which were obtained between 2004-2006, a major source of human salmonellosis. The AMR determinants were investigated by PCR, checking the presence of class 1 and class 2 integrons and 29 resistance genes. Genes sul1, blaTEM1-like, aadA2, tet(A), and dfrA12 were more prevalent (p < 0.05) within the determinants that were checked for each of these antimicrobials. Co-existence of different genes conferring resistance to the same antimicrobial was common. No differences in AMR determinants prevalence were observed between Salmonella Typhimurium and other serovars from the study. Class 1 integrons were detected in 48 of 62 isolates, again with no differences being linked to any serovar. Nine different variable regions were observed, 1000 bp/aadA2-1200 bp/blaPSE-1 (13 isolates) and blaOXA-like/aadA1 (eight isolates) were the most common. Four isolates, including S. Typhimurium (2), Salmonella Bredeney (1), and Salmonella Kapemba (1) harboured a class 2 integron 2300 bp estX-sat2-aadA1. Results from the study highlight the importance of class 1 integrons and certain genes in MDR swine Salmonella isolates. The information is of relevance for monitoring in the forthcoming scope of reduction of antibiotic usage in swine productionSIThe authors would like to express their gratitude to the Department Biological Safety, German Federal Institute for Risk Assessment, Berlin, Germany. In particular to all technicians who helped in the laboratory analysis and to the researchers Reiner Helmuth, Bukhard Malorny and Istvan Szabo who provided their advice selflessly. HA is granted by the Spanish Ministry of Economia and Competitividad IJCI-2016-3079

Detection and Genetic Diversity of Porcine Coronavirus Involved in Diarrhea Outbreaks in Spain

[EN]Porcine enteric coronaviruses include some of the most relevant viral pathogens to the swine industry such as porcine epidemic diarrhea virus (PEDV) or porcine transmissible gastroenteritis virus (TGEV) as well as several recently identified virus such as swine enteric coronavirus (SeCoV), porcine deltacoronavirus (PDCoV) or swine enteric alphacoronavirus (SeACoV). The aim of this study is the identification and characterization of enteric coronaviruses on Spanish pig farms between 2017 and 2019. The study was carried out on 106 swine farms with diarrhea outbreaks where a viral etiology was suspected by using two duplex RT-PCRs developed for the detection of porcine enteric coronaviruses. PEDV was the only coronavirus detected in our research (38.7% positive outbreaks, 41 out of 106) and neither TGEV, SeCoV, PDCoV nor SeACoV were detected in any of the samples. The complete S-gene of all the PEDV isolates recovered were obtained and compared to PEDV and SeCoV sequences available in GenBank. The phylogenetic tree showed that only PEDV of the INDEL 2 or G1b genogroup has circulated in Spain between 2017 and 2019. Three different variants were detected, the recombinant PEDV-SeCoV being the most widespread. These results show that PEDV is a relevant cause of enteric disorders in pigs in Spain while new emerging coronavirus have not been detected so far. However, the monitoring of these virus is advisable to curtail their emergence and spread.SIThis work was supported by the program from the National Institute of Agricultural and Food Research and Technology (INIA project E-RTA2015-0003-C02-02) of Spanish Government. HP, ÓM-A, and HA were supported by Spanish Government (FPU17/00466, FPU16/03485, and BEAGAL-18-106, respectively) and MG-G by Junta de Castilla y León (LE131-18)

In vitro activity of essential oils against microbial isolates from otitis externa cases in dogs

[EN] Despite the number of studies focused on the potential use of essential oils (EOs) as an alternative to conventional treatments of canine external otitis, there is controversy about their antimicrobial activity which could be explained by differences in technical or biological aspects. This study focuses on the antimicrobial activity of three single EO compounds (thymol, cinnamaldehyde and carvacrol) and two EOs (clove and oregano) against clinical isolates recovered from canine otitis externa cases (14 bacterial isolates belonging to five different genera and six Malassezia pachydermatis isolates). All compounds showed activity and cinnamaldehyde exhibited the highest bactericidal and fungicidal activity. The susceptibility was lower among bacterial isolates than fungal isolates, being this difference more evident for Gram-positive bacteria. No relationship between antibiotic multi-resistant profile and susceptibility to compounds was observed. To sum up, our results provide appropriate information about appropriate concentrations of promising candidates for the topic treatment of canine otitis.SIManuel Gómez-García was supported by grant from Junta de Castilla y León co-financed by the European Social Fund (LE131-18). Héctor Puente (FPU17/00466) and Óscar Mencía-Ares (FPU16/03485) were supported by the Spanish Ministry of Education. Héctor Argüello was supported by the Spanish Ministry of Education under the programme Beatriz Galindo (BEAGAL-18-106)

Genetic Basis and Clonal Population Structure of Antibiotic Resistance in Campylobacter jejuni Isolated From Broiler Carcasses in Belgium

Copyright © 2018 Elhadidy, Miller, Arguello, Álvarez-Ordóñez, Duarte, Dierick and Botteldoorn. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.[EN]Human campylobacteriosis is the leading food-borne zoonosis in industrialized countries. This study characterized the clonal population structure, antimicrobial resistance profiles and occurrence of antimicrobial resistance determinants of a set of Campylobacter jejuni strains isolated from broiler carcasses in Belgium. Minimum inhibitory concentrations (MICs) against five commonly-used antibiotics (ciprofloxacin, nalidixic acid, tetracycline, gentamicin, and erythromycin) were determined for 204 C. jejuni isolates. More than half of the isolates were resistant to ciprofloxacin or nalidixic acid. In contrast, a lower percentage of screened isolates were resistant to gentamicin or erythromycin. C. jejuni isolates resistant to ciprofloxacin and/or nalidixic acid were screened for the substitution T86I in the quinolone resistance determining region (QRDR) of the gyrA gene, while C. jejuni isolates resistant to tetracycline were screened for the presence of the tet(O) gene. These resistance determinants were observed in most but not all resistant isolates. Regarding resistance to erythromycin, different mutations occurred in diverse genetic loci, including mutations in the 23S rRNA gene, the rplD and rplV ribosomal genes, and the intergenic region between cmeR and cmeABC. Interestingly, and contrary to previous reports, the A2075G transition mutation in the 23S rRNA gene was only found in one strain displaying a high level of resistance to erythromycin. Ultimately, molecular typing by multilocus sequence typing revealed that two sequence types (ST-824 and ST-2274) were associated to quinolones resistance by the presence of mutations in the gene gyrA (p = 0.01). In addition, ST-2274 was linked to the CIP-NAL-TET-AMR multidrug resistant phenotype. In contrast, clonal complex CC-45 was linked to increased susceptibility to the tested antibiotics. The results obtained in this study provide better understanding of the phenotypic and the molecular basis of antibiotic resistance in C. jejuni, unraveling some the mechanisms which confer antimicrobial resistance and particular clones associated to the carriage and spread of resistance genes.SIWe would like to thank the Belgian Federal Science Policy (BELSBO) for supporting the post-doctoral fellowship of ME at WIV-ISP. The authors would like to thank Dr. Shannon Manning at the University of Michigan and Dr. Dorota Korsak at the University of Warsaw for providing positive controls for mutations in the 23S rRNA gene. Parts of this work were presented at the Twenty Seventh European conference of Clinical Microbiology and Infectious Diseases (ECCMID), April 21-25 2017, Vienna, Austria and the Twenty first Conference on Food Microbiology in Brussels, Belgium, 15-16 September 2016. AA-O acknowledges the financial support by Fundación BBVA and Ministerio de Economía y Competitividad (AGL2016-78085-P). HA is a post-doctoral researcher supported by the Juan de la Cierva Post-doctoral Trainee Program of the Spanish Ministry of Economy and Competitiveness (FJCI-2014-22877)

Combined in-vitro and on-farm evaluation of commercial disinfectants used against Brachyspira hyodysenteriae

[EN] Background: Swine dysentery (SD) is a severe infectious disease with a relevant impact on pig production usually caused by Brachyspira hyodysenteriae, although B. hampsonii causes an identical clinical picture. SD control relies on antimicrobials, good management practices and strict biosecurity with cleaning and disinfection as crucial tools to avoid the pathogen transmission. This study evaluates the in-vitro efficacy of an array of commercial disinfectants against a collection of B. hyodysenteriae isolates using broth tests. The efficacy of cleaning and disinfection protocols was also evaluated on two farms with endemic SD using surface swabs collected in emptied pens before and after cleaning and disinfection procedures, using both real-time PCR and bacterial microbiological culture. Results: Most of the commercial disinfectants evaluated were effective against all B. hyodysenteriae isolates tested, with a reduction of more than 5.00 log10 CFU/mL (bactericidal efficacy of 99.999%). However, some isolates exhibited reduced susceptibility to Virkon-S and Limoseptic disinfectants. The evaluation of cleaning and disinfection protocols on farms with SD outbreaks showed that approximately half the pens tested (n = 25) were positive by real-time PCR after pigs removal (mean B. hyodysenteriae counts 5.72 ± 1.04 log10 CFU/mL) while almost 20% of the pens remained positive after cleaning (n = 7) and disinfection (n = 5) procedures although with significantly lower, mean estimates (4.31 ± 0.43 log10 CFU/mL and 4.01 ± 0.55 log10 CFU/mL, respectively). Conclusions: These results show the efficacy of disinfectants against B. hyodysenteriae but also stress the need to implement adequately the cleaning and disinfection protocols on pig farms and review and revise their efficiency periodically.SIThis study was supported by the Ministerio de Ciencia e Innovación (PID2019-110662RB-I00) and the Castilla y León Rural Development Pro‑ gramme co-fnanced by EAFRD (47/18/125/G0

In vitro evaluation of gentamicin activity against Spanish field isolates of Brachyspira hyodysenteriae

[EN]Background: The treatment of swine dysentery (SD) has become constrained in recent years due to the limited availability of effective drugs combined with a rise in antimicrobial resistance. Gentamicin, an aminoglycoside antibiotic, is authorised for the control of this disease in several European countries but has not been extensively used so far. In this study, the in vitro susceptibility of 56 Brachyspira hyodysenteriae field isolates was evaluated against gentamicin using a broth microdilution test. The molecular basis of decreased susceptibility to gentamicin was also investigated by sequencing the 16S rRNA gene and phylogenetic relatedness by multiple-locus variable number tandem-repeat analysis (MLVA). Results: Most B. hyodysenteriae isolates presented low minimum inhibitory concentration (MIC) values to gentamicin, with a mode of 2 µg/mL, a median or MIC50 of 4 µg/mL and percentile 90 or MIC90 of 16 µg/mL. The distribution of these values over the period studied (2011–2019) did not show a tendency towards the development of resistance to gentamicin. Differences in susceptibility among isolates could be explained by two point-mutations in the 16S rRNA gene, C990T and A1185G, which were only present in isolates with high MICs. These isolates were typed in three different MLVA clusters. Analyses of co-resistance between gentamicin and antimicrobials commonly used for the treatment of SD revealed that resistance to tiamulin and valnemulin was associated with low MICs for gentamicin. Conclusions: The results provide an accurate characterisation of antimicrobial sensitivity to gentamicin and possible mechanisms of resistance in Spanish B. hyodysenteriae isolates. These findings allow us to propose gentamicin as an alternative in the antibiotic management of SD, particularly in outbreaks caused by pleuromutilin resistant isolates.SIThe authors wish to thank Benjamín Rabanal for his contribution in some parts of the research and Diana Molina for her excellent technical assistance. Clara Vega (workplace ULE-02-B) and Manuel Gómez (LE131-18) are supported by grants from Junta de Castilla y León co-financed by the European Social Fund and Youth Employment Initiative. Héctor Argüello is supported by a grant from the Spanish Ministry of Education under the programme “Beatriz Galindo” (BEAGAL-18-106). Lucía Pérez-Pérez (PRE2020-093762) and Héctor Puente (FPU17/00466) are also supported by the Spanish Ministry of Education. We fnally want to thank the reviewer for the advice and ideas provided which clearly improved the quality of the manuscript.This work was supported by Fatro Ibérica S.L