792 research outputs found
Chemimechanical Pulping of Eucalyptus Grandis
Eucayptus is currently one of the main fibrous raw materials used in the pulp and paper industry in given parts of the world. The objective of the present paper is to optimize the chemimechanical pulping conditions for Eucalyptus grandis. evaluate the pulp quality obtained, and draw conclusions regarding its potential use. The raw material used was Eucalyptus grandis industrial chips obtained at a Celulosa Argentina mill in Puerto Piray, Misiones, Argentina. For all the experiments, the chemical stage was carried out in a stainless steel digester with a liquor recirculation system. The mechanical stage was carried out in an 8-in. atmospheric disk refiner. Sodium sulfite and sodium hydroxide were added as chemical reactives. The central composite experimental design used involved five levels for the two variables studied (LL factorial design + star + central point). Three repetitions of the central point were carried out to check for errors. The variables studied were: initial amount of sodium sulfite in the wood (0.9 to 3.5% oven-dry wood) and reaction temperature (96 to 124 C). Times until maximum temperature and time at maximum temperature were, respectively, 20 and 90 minutes. A constant level of sodium hydroxide was maintained in all the experiments (1.5% oven-dry wood). Pulp evaluation was carried out using the usual characterization techniques. Chemical and physical evaluations, including optical testing, were, for the most part, done in accordance with TAPPI procedures.The results obtained indicate that the central point of the design used in our research (110 C and 2.5% oven-dry wood sulfite), appears to represent the optimal conditions for the variables studied for the chemimechanical pulping of Eucalyptus grandis. The pulps obtained could be used as furnish in printing and writing paper grades. The positive correlation between sulfonate concentration and water retention value (WRV) suggests that by increasing fiber wall swelling, the number of sites accessible to sulfonation is increased. The tensile index correlates positively with the degree of sulfonation and with the water retention value of the pulps. It decreases according to the fraction retained in a 30-mesh screen (due to the presence of numerous shives) and increases according to the fraction of fines passing through a 270-mesh screen
AACOG Region
Monthly newsletter of the Alamo Area Council of Governments describing news and events of relevance to the agencies
AACOG Region
Monthly newsletter of the Alamo Area Council of Governments describing news and events of relevance to the agencies
Is Barbero's Hamiltonian formulation a Gauge Theory of Lorentzian Gravity?
This letter is a critique of Barbero's constrained Hamiltonian formulation of
General Relativity on which current work in Loop Quantum Gravity is based.
While we do not dispute the correctness of Barbero's formulation of general
relativity, we offer some criticisms of an aesthetic nature. We point out that
unlike Ashtekar's complex SU(2) connection, Barbero's real SO(3) connection
does not admit an interpretation as a space-time gauge field. We show that if
one tries to interpret Barbero's real SO(3) connection as a space-time gauge
field, the theory is not diffeomorphism invariant. We conclude that Barbero's
formulation is not a gauge theory of gravity in the sense that Ashtekar's
Hamiltonian formulation is. The advantages of Barbero's real connection
formulation have been bought at the price of giving up the description of
gravity as a gauge field.Comment: 12 pages, no figures, revised in the light of referee's comments,
accepted for publication in Classical and Quantum Gravit
Effects of APOE4 allelic dosage on lipidomic signatures in the entorhinal cortex of aged mice
Apolipoprotein E ε4 (APOE4) is the primary genetic risk factor for the late-onset form of Alzheimer's disease (AD). Although the reason for this association is not completely understood, researchers have uncovered numerous effects of APOE4 expression on AD-relevant brain processes, including amyloid beta (Aβ) accumulation, lipid metabolism, endosomal-lysosomal trafficking, and bioenergetics. In this study, we aimed to determine the effect of APOE4 allelic dosage on regional brain lipid composition in aged mice, as well as in cultured neurons. We performed a targeted lipidomic analysis on an AD-vulnerable brain region (entorhinal cortex; EC) and an AD-resistant brain region (primary visual cortex; PVC) from 14-15 month-old APOE3/3, APOE3/4, and APOE4/4 targeted replacement mice, as well as on neurons cultured with conditioned media from APOE3/3 or APOE4/4 astrocytes. Our results reveal that the EC possesses increased susceptibility to APOE4-associated lipid alterations compared to the PVC. In the EC, APOE4 expression showed a dominant effect in decreasing diacylglycerol (DAG) levels, and a semi-dominant, additive effect in the upregulation of multiple ceramide, glycosylated sphingolipid, and bis(monoacylglycerol)phosphate (BMP) species, lipids known to accumulate as a result of endosomal-lysosomal dysfunction. Neurons treated with conditioned media from APOE4/4 vs. APOE3/3 astrocytes showed similar alterations of DAG and BMP species to those observed in the mouse EC. Our results suggest that APOE4 expression differentially modulates regional neuronal lipid signatures, which may underlie the increased susceptibility of EC-localized neurons to AD pathology
Collaborative action research through technologically mediated agoras.
ABSTRACT: The study presented in this article forms part of a wider project promoting collaboration between junior researchers from different universities with the objective of rethinking and improving teaching practice in relation to the use of technology. The article describes research carried out during the 2012/13 academic year aimed at developing collaborative action research through technologically mediated agoras involving students from three Spanish universities. The main results of this study show that junior researchers improved their teaching practice through technologically mediated inside and outside agoras. In addition, the transformation of university classrooms into agoras enabled the negotiated reconstruction of knowledge for the analysis of good practice in the use of technology. Likewise, these agoras helped reduce limitations by breaking down the barriers of time, distance and resources for sharing findings and limitations between junior researchers. Furthermore, they pave the way for improvements and their implementation in learning processes during initial teacher training
Increased localization of APP-C99 in mitochondria-associated ER membranes causes mitochondrial dysfunction in Alzheimer disease
In the amyloidogenic pathway associated with Alzheimer disease (AD), the
amyloid precursor protein (APP) is cleaved by beta-secretase to generate
a 99-aa C-terminal fragment (C99) that is then cleaved by c-secretase to
generate the beta-amyloid (Ab) found in senile plaques. In previous
reports, we and others have shown that c-secretase activity is enriched
in mitochondria-associated endoplasmic reticulum (ER) membranes (MAM)
and that ER-mitochondrial connectivity and MAM function are upregulated
in AD. We now show that C99, in addition to its localization in
endosomes, can also be found in MAM, where it is normally processed
rapidly by c-secretase. In cell models of AD, however, the concentration
of unprocessed C99 increases in MAM regions, resulting in elevated
sphingolipid turnover and an altered lipid composition of both MAM and
mitochondrial membranes. In turn, this change in mitochondrial membrane
composition interferes with the proper assembly and activity of
mitochondrial respiratory supercomplexes, thereby likely contributing to
the bioenergetic defects characteristic of AD.We thank Drs. Orian Shirihai and Marc Liesa (UCLA) for assistance with
the Seahorse measurements, Dr. Huaxi Xu (Sanford Burnham Institute) for
the APP-DKO MEFs and Dr. Mark Mattson (NIH) for the PS1 knock-in mice,
Drs. Arancio and Teich for the APP-KO mice tissues used in these
studies, Dr. Hua Yang (Columbia University) for mouse husbandry, and
Drs. Marc Tambini, Ira Tabas, and Serge Przedborski for helpful
comments. This work was supported by the Fundacion Alfonso Martin
Escudero (to M.P.); the Alzheimer's Drug Discovery Foundation, the
Ellison Medical Foundation, the Muscular Dystrophy Association, the U.S.
Department of Defense W911NF-12-1-9159 and W911F-15-1-0169), and the J.
Willard and Alice S. Marriott Foundation (to E.A.S.); the U.S. National
Institutes of Health (P01-HD080642 and P01-HD032062 to E.A.S.; NS071571
and HD071593 to M.F.M.; R01-NS056049 and P50-AG008702 to G.D.P.;
1S10OD016214-01A1 to G.S.P. and F.P.M, and K01-AG045335 to E.A.-G.), the
Lucien Cote Early Investigator Award in Clinical Genetics from the
Parkinson's Disease Foundation (PDF-CEI-1364 and PDF-CEI-1240) to
C.G.-L., and National Defense Science and Engineering Graduate
Fellowship (FA9550-11-C-0028) to R.R.A.S
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Financial Analysis of Experimental Releases Conducted at Glen Canyon Dam During Water Year 2011
This report examines the financial implications of experimental flows conducted at the Glen Canyon Dam (GCD) in water year 2011. It is the third report in a series examining financial implications of experimental flows conducted since the Record of Decision (ROD) was adopted in February 1997 (Reclamation 1996). A report released in January 2011 examined water years 1997 to 2005 (Veselka et al. 2011), and a report released in August 2011 examined water years 2006 to 2010 (Poch et al. 2011). An experimental release may have either a positive or negative impact on the financial value of energy production. This study estimates the financial costs of experimental releases, identifies the main factors that contribute to these costs, and compares the interdependencies among these factors. An integrated set of tools was used to compute the financial impacts of the experimental releases by simulating the operation of the GCD under two scenarios, namely, (1) a baseline scenario that assumes both that operations comply with the ROD operating criteria and the experimental releases that actually took place during the study period, and (2) a 'without experiments' scenario that is identical to the baseline scenario of operations that comply with the GCD ROD, except it assumes that experimental releases did not occur. The Generation and Transmission Maximization (GTMax) model was the main simulation tool used to dispatch GCD and other hydropower plants that comprise the Salt Lake City Area Integrated Projects (SLCA/IP). Extensive data sets and historical information on SLCA/IP powerplant characteristics, hydrologic conditions, and Western Area Power Administration's (Western's) power purchase prices were used for the simulation. In addition to estimating the financial impact of experimental releases, the GTMax model was also used to gain insights into the interplay among ROD operating criteria, exceptions that were made to criteria to accommodate the experimental releases, and Western operating practices. Experimental releases conducted in water year 2011 resulted only in financial costs; the total cost of all experimental releases was about $622,000
The making of a mammalian peroxisome, version 2.0: mitochondria get into the mix
This is the author accepted manuscript. The final version is available from Nature Publishing Group via the DOI in this record.A recent report from the laboratory of Heidi McBride (McGill University) presents a role for mitochondria in the de novo biogenesis of peroxisomes in mammalian cells (1). Peroxisomes are essential organelles responsible for a wide variety of biochemical functions, from the generation of bile, to plasmalogen synthesis, reduction of peroxides, and the oxidation of very long chain fatty acids (2). Like mitochondria, peroxisomes proliferate primarily through growth and division of pre-existing peroxisomes (3-6). However, unlike mitochondria, peroxisomes do not fuse (5,7); further, and perhaps most
importantly, they can also be born de novo, a process thought to occur through the generation of pre-peroxisomal vesicles that originate from the endoplasmic reticulum (reviewed in (8,9). De novo peroxisome biogenesis has been extensively studies in yeast, with a major focus on the role of the ER in this process. Comprehensive studies in mammalian cells are, however, scarce (5,10-12). By exploiting patient cells lacking mature peroxisomes, Sugiura et al. (1) now assign a role to ER and mitochondria in de novo mammalian peroxisome biogenesis by showing that the formation of immature preperoxisomes occurs through the fusion of Pex3- / Pex14-containing mitochondriaderived vesicles with Pex16-containing ER-derived vesicles
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