Bioadhesive properties of Gantrez nanoparticles

Bioadhesive nanoparticles have been proposed as carriers for the oral delivery of poorly available drugs and facilitate the use of this route. This work summarises some experiments describing the bioadhesive potential of Gantrez nanoparticles fluorescently labeled with rhodamine B isothiocyanate. The adhesive potential of Gantrez was found to be stronger when folded as nanoparticles than in the solubilised form. Conventional nanoparticles displayed a tropism for the upper areas of the gastrointestinal tract, with a maximum of adhesion 30 min post-administration and a decrease in the adhered fraction along the time depending on the given dose. The cross-linkage of nanoparticles with increasing amounts of 1,3-diaminopropane stabilised the resulting carriers and prolonged their half-life in an aqueous environment; although, the adhesive capacity of nanoparticles, the intensity and the relative duration of the adhesive interactions within the gut as a function of the cross-linking degree. Finally, nanoparticles were coated with either gelatin or albumin. In the first case, the presence of gelatin dramatically decreased the initial capacity of these carriers to interact with the gut mucosa and the intensity of these phenomenons. In the latter, bovine serum albumin coated nanoparticles (BSA-NP) showed an important tropism for the stomach mucosa without further significant distribution to other parts of the gut mucosa

Chemical analysis of pottery demonstrates prehistoric origin for high-altitude alpine dairying

The European high Alps are internationally renowned for their dairy produce, which are of huge cultural and economic significance to the region. Although the recent history of alpine dairying has been well studied, virtually nothing is known regarding the origins of this practice. This is due to poor preservation of high altitude archaeological sites and the ephemeral nature of transhumance economic practices. Archaeologists have suggested that stone structures that appear around 3,000 years ago are associated with more intense seasonal occupation of the high Alps and perhaps the establishment of new economic strategies. Here, we report on organic residue analysis of small fragments of pottery sherds that are occasionally preserved both at these sites and earlier prehistoric rock-shelters. Based mainly on isotopic criteria, dairy lipids could only be identified on ceramics from the stone structures, which date to the Iron Age (ca. 3,000 - 2,500 BP), providing the earliest evidence of this practice in the high Alps. Dairy production in such a marginal environment implies a high degree of risk even by today’s standards. We postulate that this practice was driven by population increase and climate deterioration that put pressure on lowland agropastoral systems and the establishment of more extensive trade networks, leading to greater demand for highly nutritious and transportable dairy products

Análisis de clúster de los estilos de vida de los estudiantes universitarios

Introducción: Existen estudios que demuestran que en la etapa universitaria se abandonan ciertos hábitos saludables. Y, está ampliamente demostrado que determinadas conductas de riesgo como el tabaquismo, una dieta poco saludable, una conducta sedentaria o un excesivo consumo de alcohol pueden contribuir de forma independiente a incrementar la morbimortalidad. No obstante, se ha estudiado poco la agrupación entre los diferentes factores de riesgo. Objetivo general: Determinar el grado de conglomeración de los mayores factores de riesgo (consumo de tabaco, alcohol, baja adherencia a la dieta mediterránea y sedentarismo) en estudiantes universitarios. Material y método: Estudio analítico transversal mediante un análisis de clúster (n=444), estratificado por ramas de conocimiento. Resultados: Se han identificados cuatro clusters dentro de la muestra de estudiantes universitarios basada en las variables de interés (adherencia a la dieta mediterránea, consumo de alcohol, estado de salud, nivel de estrés, calidad de vida, IMC y nivel de actividad física): Clúster 1 (Moderadamente saludables / Grupo de riesgo moderado); Clúster 2 (No saludables / Grupo de riesgo moderado); Clúster 3 (Saludables / Grupo de riesgo bajo); y, Clúster 4 (No saludables / Grupo de riesgo alto). Los resultados reafirman la idea de que los comportamientos de salud no deben considerarse de forma aislada unos de otros, ya que demuestran una tendencia a la conglomeración de los estilos de vida saludables versus los estilos de vida no saludables. Conclusiones: Se observa una fuerte asociación entre los estilos de vida de alimentación y actividad física, destacando que una buena adherencia a la dieta mediterránea se asocia con unos niveles más altos de ejercicio físico, con un menor nivel de estrés, un buen estado de salud y una buena calidad de vida

Bioadhesive properties of Gantrez nanoparticles

Bioadhesive nanoparticles have been proposed as carriers for the oral delivery of poorly available drugs and facilitate the use of this route. This work summarises some experiments describing the bioadhesive potential of Gantrez nanoparticles fluorescently labeled with rhodamine B isothiocyanate. The adhesive potential of Gantrez was found to be stronger when folded as nanoparticles than in the solubilised form. Conventional nanoparticles displayed a tropism for the upper areas of the gastrointestinal tract, with a maximum of adhesion 30 min post-administration and a decrease in the adhered fraction along the time depending on the given dose. The cross-linkage of nanoparticles with increasing amounts of 1,3-diaminopropane stabilised the resulting carriers and prolonged their half-life in an aqueous environment; although, the adhesive capacity of nanoparticles, the intensity and the relative duration of the adhesive interactions within the gut as a function of the cross-linking degree. Finally, nanoparticles were coated with either gelatin or albumin. In the first case, the presence of gelatin dramatically decreased the initial capacity of these carriers to interact with the gut mucosa and the intensity of these phenomenons. In the latter, bovine serum albumin coated nanoparticles (BSA-NP) showed an important tropism for the stomach mucosa without further significant distribution to other parts of the gut mucosa

Unraveling the phylogenetic relationships of the extinct bovid Myotragus balearicus Bate 1909 from the Balearic Islands

Myotragus balearicus was the last living representative of an extinct caprine lineage endemic to the Balearic Islands (Western Mediterranean), which became extinct following the arrival of humans around 4300 years ago. The tribal attribution of Myotragus based on morphological analyses has been complicated due to its unusual morphological characteristics, though most studies agree on its inclusion within Caprini (also including modern sheep and goats). Analyses of short fragments of ancient mitochondrial DNA have suggested Ovis (comprising extant sheep) as sister taxon to Myotragus, although other authors have suggested alternative placements within Caprini using the same data. In the present study, we present a complete high-depth mitochondrial genome of M. balearicus, which allowed us to test the previously proposed Ovis/Myotragus clade and revealed a closer relationship between Myotragus and the takin (Budorcas taxicolor). The results of our molecular clock analyses suggested that the split between Myotragus and Budorcas occurred around 7.1 Mya, which is compatible with the arrival of the ancestor of Myotragus to the Balearic Islands during the Messinian Salinity Crisis (5.97–5.33 Mya). We also conducted a preliminary phylogeographic analysis of M. balearicus in Mallorca, which revealed weak spatial and temporal structure within the population during the Holocene.Pere Bover, Bastien Llamas, Kieren J. Mitchell, Vicki A. Thomson, Josep Antoni Alcover, Carles Lalueza-Fox, Alan Cooper, Joan Pon