Perspective Chapter: Accelerating Demand-Led Tomato Breeding for Emerging Markets in Africa

Tomato production in Africa has increased due to increased population, rising consumer demands for nutritious and healthy food and potential use of improved technologies. Demand-led’ plant breeding puts producers and consumers at the heart of research and development involving stakeholders even before the research starts. These ‘stakeholders’ are not only farmers but key actors along the tomato value chain. They influence how the tomato is traded as: fresh food and processing product. This chapter focuses on different approaches to fast-track tomato breeding so as to contribute to the transformation of African agriculture by enabling small scale farmers to compete in local and regional markets, by increasing the availability and adoption of high performing tomato varieties that meet market demands. It further outlines development of varieties that meet farmer needs, consumer preferences, and market demand in Africa. These new varieties are designed to meet client needs by connecting plant breeders with crop value chains, seed distribution organizations, and encouraging enterprise and entrepreneurship in transforming agriculture in Africa. Lastly, it outlines the prospects and challenges associated with demand-led breeding of tomato and offers suggestions to increase food security in Africa

Mitigating against Sclerotinia Diseases in Legume Crops: A Comprehensive Review

Legumes are essential foods for man and animal. They contribute to food security globally. However, they are negatively affected by Sclerotinia diseases caused by Sclerotinia sclerotiorum, which infects over 600 plant species. There is a limited number of review studies on the management of the Sclerotinia sclerotiorum disease in legume crops. Here, we explore earlier studies on the occurrences, yield losses, and other negative effects caused by Sclerotinia spp. in legumes. Additionally, we studied the various strategies used in controlling Sclerotinia sclerotiorum diseases in legume crops. We conclude that the impact of Sclerotinia diseases on legume crops causes an economic loss, as it reduces their quality and yield. Among the management strategies explored, genetic control is challenging due to the limited resistance among germplasm, while biological agents show promising results. Fungicide application is effective during outbreaks of Sclerotinia diseases. Lastly, this review has uncovered gaps in the current knowledge regarding the alleviation of Sclerotinia diseases in legume crops

NAC Transcription Factor GmNAC12 Improved Drought Stress Tolerance in Soybean

NAC transcription factors (TFs) could regulate drought stresses in plants; however, the function of NAC TFs in soybeans remains unclear. To unravel NAC TF function, we established that GmNAC12, a NAC TF from soybean (Glycine max), was involved in the manipulation of stress tolerance. The expression of GmNAC12 was significantly upregulated more than 10-fold under drought stress and more than threefold under abscisic acid (ABA) and ethylene (ETH) treatment. In order to determine the function of GmNAC12 under drought stress conditions, we generated GmNAC12 overexpression and knockout lines. The present findings showed that under drought stress, the survival rate of GmNAC12 overexpression lines increased by more than 57% compared with wild-type plants, while the survival rate of GmNAC12 knockout lines decreased by at least 46%. Furthermore, a subcellular localisation analysis showed that the GmNAC12 protein is concentrated in the nucleus of the tobacco cell. In addition, we used a yeast two-hybrid assay to identify 185 proteins that interact with GmNAC12. Gene ontology (GO) and KEGG analysis showed that GmNAC12 interaction proteins are related to chitin, chlorophyll, ubiquitin–protein transferase, and peroxidase activity. Hence, we have inferred that GmNAC12, as a key gene, could positively regulate soybean tolerance to drought stress

Analysis of the determinants of sustainable agricultural technologies adoption in tea production in China: a systematic review

Agriculture in China is undergoing a swift transformation to modify labour relocation. This change is spearheaded by commercial farms and other Chinese government interventions thereby promoting agricultural production and rural growth. The input-dependent industrial model of agriculture is also considered in conflict with attaining a safe and healthy environment for its inhabitants. The current approaches initiated in the tea sector have witnessed several enriched sustainable agricultural technologies (SATs). The SATs seek to increase yield while ensuring agricultural sustainability via ecological strategies. However, reports have shown farmers have low desire resulting in a lower acceptance rate of SATs among Chinese farmers. We provide a systematic review with a focus on the key determinants of SATs adoption rate in tea production in China from 2000 to 2022. The results revealed the significance and suitability of different approaches in establishing farmers’ adoption behaviour. The specificity of farmers’ SAT adoption behaviour is essentially not compromised by any codified variables but the interrelationship among the determinants, including socio-demographic, agroecological and environmental, technological, institutional, and social networks, and informational, and farmer behavioural factors. We recommend that the government increase awareness of SATs to promote public demand for agricultural sustainability, particularly in developing countries

Identification of Novel Genomic Regions for Bacterial Leaf Pustule (BLP) Resistance in Soybean (<i>Glycine max</i> L.) via Integrating Linkage Mapping and Association Analysis

Bacterial leaf pustule (BLP), caused by Xanthornonas axonopodis pv. glycines (Xag), is a worldwide disease of soybean, particularly in warm and humid regions. To date, little is known about the underlying molecular mechanisms of BLP resistance. The only single recessive resistance gene rxp has not been functionally identified yet, even though the genotypes carrying the gene have been widely used for BLP resistance breeding. Using a linkage mapping in a recombinant inbred line (RIL) population against the Xag strain Chinese C5, we identified that quantitative trait locus (QTL) qrxp–17–2 accounted for 74.33% of the total phenotypic variations. We also identified two minor QTLs, qrxp–05–1 and qrxp–17–1, that accounted for 7.26% and 22.26% of the total phenotypic variations, respectively, for the first time. Using a genome-wide association study (GWAS) in 476 cultivars of a soybean breeding germplasm population, we identified a total of 38 quantitative trait nucleotides (QTNs) on chromosomes (Chr) 5, 7, 8, 9,15, 17, 19, and 20 under artificial infection with C5, and 34 QTNs on Chr 4, 5, 6, 9, 13, 16, 17, 18, and 20 under natural morbidity condition. Taken together, three QTLs and 11 stable QTNs were detected in both linkage mapping and GWAS analysis, and located in three genomic regions with the major genomic region containing qrxp_17_2. Real-time RT-PCR analysis of the relative expression levels of five potential candidate genes in the resistant soybean cultivar W82 following Xag treatment showed that of Glyma.17G086300, which is located in qrxp–17–2, significantly increased in W82 at 24 and 72 h post-inoculation (hpi) when compared to that in the susceptible cultivar Jack. These results indicate that Glyma.17G086300 is a potential candidate gene for rxp and the QTLs and QTNs identified in this study will be useful for marker development for the breeding of Xag-resistant soybean cultivars