Molecular Identification of Anisakis larvae (Nematode: Anisakidae) from marine fishes collected from three Korean sea waters

Anisakis infects humans who eat raw seafood such as squid, mackerel, and conger, which is considered as one of the parasitic diseases in Korea. Molecular study for Anisakis L3 larvae was performed to identify the species of Anisakis. Molecular analysis for Anisakis L3 larvae was performed to identify the species of Anisakis and by using the DNA alignment tool to identify the DNA sequence relation. Total 98 Anisakis type I L3 larvae were collected from 63 fishes among three kinds of fish species (mackerel (Scomberomorus japonicus), anago (Conger myriaster), and yellow croaker (Larimichthys polyactis)) that came from different location near sea areas (Namhae-gun, Uljin-gun, Daecheon-si, Pohang-si and Seoul). DNA were extracted from Anisakis and ITS gene (forward primer: Nc5 (5’-GTAGGTGAACCTGCGGAAGGATCATT-3’); reverse primer: Nc2 (5’-TTAGTTTCTTTTCCTCCGCT-3’)) was amplified using PCR and sequenced. By using some method that considered for the species identification strategies, such as: Sequence analysing (Anisakis pegreffi (GGCAGAGTC) => C/C allele and Anisakis simplex (GGCAGAGTT) => T/T allele) and the strategy to find the rest total of unidentified in the first strategy by using nucleotide identity percentage: A. pegreffi (AB277823), A. simplex (AB277822), P. decipiens (AB277824) and H. aduncum (AB277826). It was found that Anisakis pegreffi was the most predominant Anisakis species from all fish species and in all Korean locations with 67 as a result of the total. And in the result of nucleotide identity percentage for analyzed the rest 31 samples that not identified during the sequence procedure, based on matched result, 1 were more matched to A. simplex, 25 to A. pegreffi and 5 to H. aduncum.open석

Continuidade dos planos de saúde em caso de demissão sem justa causa e aposentadoria

Trabalho de Conclusão de Curso apresentado ao Departamento Acadêmico de Direito da Fundação Universidade Federal de Rondônia, campus Professor Francisco Gonçalves Quiles, para obtenção do título de Bacharel em Direito. Orientador Prof. Me. Gilson Tetsuo MiyakavaO presente trabalho tem como objetivo geral apresentar as hipóteses de continuidade dos planos privados de assistência à saúde em caso de demissão sem justa causa ou aposentadoria. Para tanto, foi feito um breve histórico das políticas de saúde e a evolução dos contratos de planos de saúde – da total ausência de normas específicas, à aplicação do Código de Defesa do Consumidor até a Lei 9.656/98 e as normas da Agência Nacional de Saúde Suplementar, agência reguladora do setor e os limites do seu poder normativo. O objetivo específico do presente trabalho é o de verificar as condições necessárias para que o beneficiário dos contratos coletivos empresariais continue usufruindo as coberturas assistenciais de que gozava quando da vigência do contrato de trabalho, nos casos de demissão sem justa causa ou aposentadoria. Para a elaboração do estudo foi usado o método dogmático realizando um estudo por meio de pesquisas bibliográficas, de livros doutrinários, jurisprudências pátrias, leis específicas e artigos científicos atinentes ao tema

Application of Quality by Design in a Commercialized Lyophilized Vaccine

The pharmaceutical industry has being implementing regulatory practices to assure that consumers obtain products with quality, safety, and efficacy. The use of Quality by Design (QbD) for products on development has increased though the years to avoid issues related to quality parameters and has been suggested by Regulatory Agencies to standardize globally the documentation for the registration of new products. Although the concepts of QbD gain importance, it is still not a widespread practice to existing systems and products already on the market. This work aims to propose a case study with a vaccine using QbD concepts on the lyophilization unit operation production step to provide robustness and increase efficiency, leading to a lyophilization cycle time reduction. To this end, a reverse way of the use of QbD principles were applied based on historical batches database, down scale experiments, and finally in industrial scale to establish new boundaries in the lyophilization cycle. Experimental batches samples were analyzed through accelerated and real time stability study. At the end, this case became a possibility to establish new ranges to lyophilization process predicting risks and assure robustness to this production step with the maintenance of quality and safety of vaccine

Strategic environmental sensitivity mapping for oil spill contingency planning in the Peruvian marine-coastal zone

Major oil spills can cause significant impacts on marine-coastal zones, particularly on areas with a high oil spill risk, which combine a high oil spill hazard-high likelihood of oil stranding at high concentrations, and a high environmental sensitivity-high concentration of highly sensitive ecological and socioeconomic resources. In this context, a straightforward multicriteria methodology is proposed to determine the second factor of the oil spill risk, namely the strategic environmental sensitivity (SES), in 68 sectors covering the entire Peruvian marine-coastal zone. The methodology comprised the weighted integration of physical, biological, and socioeconomic sensitivity indicators based on their relevance in surface marine oil spills and the Peruvian ecological and socioeconomic context. As a result, relative SES levels from very low to very high were assigned to the sectors. To demonstrate the SES applicability, an oil spill risk assessment at a screening level was performed in a selected sector with current oil production activities. The oil beaching likelihood of worst-case discharge scenarios modelled for January 2021 was used to determine an overall screening oil spill hazard level in the selected sector, while a matrix relating the SES and hazard determined the screening oil spill risk. The results can be used as a decision-support tool to enhance the oil spill contingency planning in Peru or be used in other relevant processes such as the integrated coastal zone management, the marine spatial planning, or the contingency planning of other liquid contaminants. In addition, the proposed methodologies can be adapted to different local and international contexts and scales

Report AtlantOS fitness to Oil spill products

Assessment of the observing system fitness for purpose for the hazard mappin

Brazilian Pediatric Reference Data For Quantitative Ultrasound Of Phalanges According To Gender, Age, Height And Weight

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Aims To establish normative data for phalangeal quantitative ultrasound (QUS) measures in Brazilian students. Methods The sample was composed of 6870 students (3688 females and 3182 males), aged 6 to 17 years. The bone status parameter, Amplitude Dependent Speed of Sound (AD-SoS) was assessed by QUS of the phalanges using DBM Sonic BP (IGEA, Carpi, Italy) equipment. Skin color was obtained by self-evaluation. The LMS method was used to derive smoothed percentiles reference charts for AD-SoS according to sex, age, height and weight and to generate the L, M, and S parameters. Results Girls showed higher AD-SoS values than boys in the age groups 7-16 (p<0.001). There were no differences on AD-SoS Z-scores according to skin color. In both sexes, the obese group showed lower values of AD-SoS Z-scores compared with subjects classified as thin or normal weight. Age (r(2) = 0.48) and height (r(2) = 0.35) were independent predictors of AD-SoS in females and males, respectively. Conclusion AD-SoS values in Brazilian children and adolescents were influenced by sex, age and weight status, but not by skin color. Our normative data could be used for monitoring AD-SoS in children or adolescents aged 6-17 years.106Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)FAPESP [2006/01978-0, 2011/23460-1, 2002/13021-1, 2012/16778-8

Mental and psychosocial predictors of toothache in Brazilian dam rupture: a cross-sectional study

Introduction: The burst of Fundão's dam in Mariana, Brazil, was one of the most significant technological disasters and, for instance, may be associated with negative consequences in oral and mental health. Objective: The present study aimed to investigate the toothache prevalence and the associated factors in disaster survivors. Method: This research is an observational, cross-sectional study carried out as a health household survey with the Mariana dam rupture survivors. Results: the study included 225 adults; age mean 45.5±17.8 years. In univariate analysis, the prevalence of toothache was 16.9% and was higher in women (20.1% versus 11.1%, p= 0.097) subjects with generalized anxiety disorders (GAD) (57.9% versus 25.8%, p≥0.0001), major depressive disorder (MDD) (50 versus 24.6%, p=0.003), post-traumatic stress disorder (PTSD) (26.3 versus 9%, p=0.006), a lower social support satisfaction (SSSS). Multivariate Data Analysis (MVA) results reveal how individuals dissatisfied with their oral appearance were 3.2 times more likely to present with toothaches (p=0.004, 95% CI-1.46-7.02). Of those affected by the disaster, survivors diagnosed with GAD were 2.5 times more likely to present tooth aches (p=0.019, 95% CI=1.16-5.48). Ultimately, the likelihood of an individual presenting with toothaches after the disaster rose to 4% to each point in the SSSS (p.=0.026,95% CI=1.005-1.081). Conclusion: We found that post-disaster survivors in Brazil presented with an elevated prevalence of toothache, and this oral symptom correlated with other dental impairments, anxiety disorder, and social support