Recycle Glass

Increasing the circularity of flat glass does not only mean to collect glass cullet from internal and pre- consumer processes. It also means to use glass cullet from the post- consumer applications, such as residential or commercial buildings. The flat glass industry is currently in a transformation phase to reduce its CO2 emissions. To produce Low Carbon Glass with reduced carbon emissions generated during the production of float glass, a holistic approach is applied. Among these, one of the pillars is the increased use of cullet. Cullet comes from different sources: internal cullet, pre- and post- consumer cullet. In order to be able to use cullet in float glass production, it must be of high quality. Since there are a large number of architectural glass products and glass types, it is essential to collect and sort in the most adequate wa

A next generation vaccine against human rabies based on a single dose of a chimpanzee adenovirus vector serotype C

Rabies, caused by RNA viruses in the Genus Lyssavirus, is the most fatal of all infectious diseases. This neglected zoonosis remains a major public health problem in developing countries, causing the death of an estimated 25,000-159,000 people each year, with more than half of them in children. The high incidence of human rabies in spite of effective vaccines is mainly linked to the lack of compliance with the complicated administration schedule, inadequacies of the community public health system for local administration by the parenteral route and the overall costs of the vaccine. The goal of our work was the development of a simple, affordable and effective vaccine strategy to prevent human rabies virus infection. This next generation vaccine is based on a replication-defective chimpanzee adenovirus vector belonging to group C, ChAd155-RG, which encodes the rabies glycoprotein (G). We demonstrate here that a single dose of this vaccine induces protective efficacy in a murine model of rabies challenge and elicits strong and durable neutralizing antibody responses in vaccinated non-human primates. Importantly, we demonstrate that one dose of a commercial rabies vaccine effectively boosts the neutralizing antibody responses induced by ChAd155-RG in vaccinated monkeys, showing the compatibility of the novel vectored vaccine with the current post-exposure prophylaxis in the event of rabies virus exposure. Finally, we demonstrate that antibodies induced by ChAd155-RG can also neutralize European bat lyssaviruses 1 and 2 (EBLV-1 and EBLV-2) found in bat reservoirs

Correction: A next generation vaccine against human rabies based on a single dose of a chimpanzee adenovirus vector serotype C.

[This corrects the article DOI: 10.1371/journal.pntd.0008459.]

Cosmic ray oriented performance studies for the JEM-EUSO first level trigger

JEM-EUSO is a space mission designed to investigate Ultra-High Energy Cosmic Rays and Neutrinos (E > 5 ⋅ 1019 eV) from the International Space Station (ISS). Looking down from above its wide angle telescope is able to observe their air showers and collect such data from a very wide area. Highly specific trigger algorithms are needed to drastically reduce the data load in the presence of both atmospheric and human activity related background light, yet retain the rare cosmic ray events recorded in the telescope. We report the performance in offline testing of the first level trigger algorithm on data from JEM-EUSO prototypes and laboratory measurements observing different light sources: data taken during a high altitude balloon flight over Canada, laser pulses observed from the ground traversing the real atmosphere, and model landscapes reproducing realistic aspect ratios and light conditions as would be seen from the ISS itself. The first level trigger logic successfully kept the trigger rate within the permissible bounds when challenged with artificially produced as well as naturally encountered night sky background fluctuations and while retaining events with general air-shower characteristics

Collaborations between MNEs and entrepreneurial ventures. A study on Open Innovability in the energy sector

As environmental challenges continue to mount, sustainable innovation has become a pressing need. Small entrepreneurial ventures and multinational enterprises (MNEs) have started to join forces to address this challenge. However, the dynamics of this collaboration, especially from the standpoint of smaller ventures, remain largely unexplored. In this paper, we shed light on the division of entrepreneurial labor in global interfirm networks by investigating the collaboration between MNEs and entrepreneurial ventures in the energy industry, addressing SDG goal 7. We conduct semi-structured interviews with six ventures and employ grounded theory to analyze the data. Our findings indicate that smaller ventures need to be ready to overcome obstacles when collaborating with MNEs, as the expected benefits drive the partnership. Notably, the ventures express interest in continuing the collaboration and share similar paths of growth, including accidental internationalization

Conversaciones vitales: Trabajadores territoriales, organizaciones sociales y juventudes. Una mirada sobre el Programa Nueva Oportunidad

Este libro es el resultado de un estudio que se propuso indagar las implicancias del Programa Nueva Oportunidad en el entramado social de los Distritos Noroeste, Oeste y Sur de la ciudad de Rosario, desde la perspectiva de los acompañantes territoriales. Específicamente, se abocó a analizar el rol de dichos acompañantes, su vínculo con las organizaciones sociales, las articulaciones con el territorio y los jóvenes del barrio; las representaciones de los acompañantes en relación con la implementación del Programa Nueva Oportunidad, así como su puesta en diálogo con las perspectivas de los otros actores involucrados (matriciales, referentes, capacitadores y jóvenes).Fil: Mansilla, Andrea. Universidad Nacional de Rosario. Facultad de Ciencia Política y Relaciones Internacionales. Escuela de Ciencia Política; Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias; Argentina. Universidad Tecnológica Nacional; ArgentinaFil: Di Filippo, Marilé. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario; Argentina. Universidad Nacional de Rosario. Facultad de Ciencia Política y Relaciones Internacionales. Escuela de Ciencia Política; Argentina. Universidad de Buenos Aires; Argentina. Universidad Nacional de Rosario. Centro de Estudios Interdisciplinarios; ArgentinaFil: Daneri, Mariela. Universidad Nacional de Rosario. Facultad de Ciencia Política y Relaciones Internacionales. Escuela de Ciencia Política; ArgentinaFil: Contino, Paula Silvina. Universidad Nacional de Rosario. Facultad de Ciencia Política y Relaciones Internacionales. Escuela de Ciencia Política; Argentina. Ministerio de Educación, Cultura, Ciencia y Tecnología. Consejo Interuniversitario Nacional; ArgentinaFil: Alberti, Cristian Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario; Argentina. Universidad Nacional de Rosario. Facultad de Ciencia Política y Relaciones Internacionales. Escuela de Ciencia Política; ArgentinaFil: Guardatti, Julieta. Universidad Nacional de Rosario. Facultad de Ciencia Política y Relaciones Internacionales. Escuela de Ciencia Política; ArgentinaFil: Angelomé, Victoria. Universidad Nacional de Rosario. Facultad de Ciencia Política y Relaciones Internacionales. Escuela de Ciencia Política; ArgentinaFil: Chiarito, Celina. Universidad Nacional de Rosario. Facultad de Ciencia Política y Relaciones Internacionales. Escuela de Ciencia Política; Argentina. Biblioteca Popular Empalme Norte; ArgentinaFil: Marinaro, Agostina. Universidad Nacional de Rosario. Facultad de Ciencia Política y Relaciones Internacionales. Escuela de Ciencia Política; ArgentinaFil: Di Santo, Antonella. Universidad Nacional de Rosario. Facultad de Ciencia Política y Relaciones Internacionales. Escuela de Ciencia Política; Argentin

Improved memory CD8 T cell response to delayed vaccine boost is associated with a distinct molecular signature.

Effective secondary response to antigen is a hallmark of immunological memory. However, the extent of memory CD8 T cell response to secondary boost varies at different times after a primary response. Considering the central role of memory CD8 T cells in long-lived protection against viral infections and tumors, a better understanding of the molecular mechanisms underlying the changing responsiveness of these cells to antigenic challenge would be beneficial. We examined here primed CD8 T cell response to boost in a BALB/c mouse model of intramuscular vaccination by priming with HIV-1 gag-encoding Chimpanzee adenovector, and boosting with HIV-1 gag-encoding Modified Vaccinia virus Ankara. We found that boost was more effective at day(d)100 than at d30 postprime, as evaluated at d45 post-boost by multi-lymphoid organ assessment of gag-specific CD8 T cell frequency, CD62L-expression (as a guide to memory status) and in vivo killing. RNA-sequencing of splenic gag-primed CD8 T cells at d100 revealed a quiescent, but highly responsive signature, that trended toward a central memory (CD62L+) phenotype. Interestingly, gag-specificCD8Tcell frequency selectively diminished in the blood at d100, relative to the spleen, lymph nodes and bone marrow. These results open the possibility to modify prime/ boost intervals to achieve an improved memory CD8 T cell secondary response

Harmaline to Human Mitochondrial Caseinolytic Serine Protease Activation for Pediatric Diffuse Intrinsic Pontine Glioma Treatment

Diffuse intrinsic pontine glioma (DIPG), affecting children aged 4-7 years, is a rare, aggressive tumor that originates in the pons and then spreads to nearby tissue. DIPG is the leading cause of death for pediatric brain tumors due to its infiltrative nature and inoperability. Radiotherapy has only a palliative effect on stabilizing symptoms. In silico and preclinical studies identified ONC201 as a cytotoxic agent against some human cancer cell lines, including DIPG ones. A single-crystal X-ray analysis of the complex of the human mitochondrial caseinolytic serine protease type C (hClpP) and ONC201 (PDB ID: 6DL7) allowed hClpP to be identified as its main target. The hyperactivation of hClpP causes damage to mitochondrial oxidative phosphorylation and cell death. In some DIPG patients receiving ONC201, an acquired resistance was observed. In this context, a wide program was initiated to discover original scaffolds for new hClpP activators to treat ONC201-non-responding patients. Harmaline, a small molecule belonging to the chemical class of β-carboline, was identified through Fingerprints for Ligands and Proteins (FLAP), a structure-based virtual screening approach. Molecular dynamics simulations and a deep in vitro investigation showed interesting information on the interaction and activation of hClpP by harmaline

Safety and immune response kinetics of GRAd-COV2 vaccine: phase 1 clinical trial results

Despite the successful deployment of efficacious vaccines and therapeutics, the development of novel vaccines for SARS-CoV-2 remains a major goal to increase vaccine doses availability and accessibility for lower income setting. We report here on the kinetics of Spike-specific humoral and T-cell response in young and old volunteers over 6 months follow-up after a single intramuscular administration of GRAd-COV2, a gorilla adenoviral vector-based vaccine candidate currently in phase-2 of clinical development. At all three tested vaccine dosages, Spike binding and neutralizing antibodies were induced and substantially maintained up to 3 months, to then contract at 6 months. Potent T-cell responses were readily induced and sustained throughout the study period, with only minor decline. No major differences in immune response to GRAd-COV2 vaccination were observed in the two age cohorts. In light of its favorable safety and immunogenicity, GRAd-COV2 is a valuable candidate for further clinical development and potential addition to the COVID-19 vaccine toolbox to help fighting SARS-CoV-2 pandemic