Study of evolution, expression and function of Nitric Oxide Synthases in the cephalochordate Branchiostoma lanceolatum

Nitric oxide (NO) is a highly reactive, diffusible gas, essential for many physiological functions including neurotransmission, learning and memory, cardiovascular homeostasis, angiogenesis, host defense through immune response, cell migration, and apoptosis. In vertebrates, NO is produced by the enzymatic conversion of L-arginine by three distinct Nitric Oxide Synthase (NOS) that have been identified as products of different genes with distinct expression patterns, cellular localization, regulation, catalytic properties and inhibitor sensitivity. The pathway of NO formation is one of the oldest bioregulatory systems, highly conserved in metazoan. Comparative studies in different model systems using non-vertebrate organisms, especially basal chordates, are very useful. Because it is likely that the basic primary roles will be evolutionary conserved, the chordate amphioxus is the best available stand-in for the ancestor of the vertebrates. Importantly, amphioxus has a body plan, central nervous system, circulatory system and genome that are vertebrate-like, but simpler. In addition, in comparison to vertebrates, amphioxus has the same spectrum of gene families, but has markedly fewer genes per family (Holland et al., 2008; Putnam et al., 2008). This relative genomic simplicity makes amphioxus an especially favorable prospect for functional studies of signaling networks and other physiological processes. Amphioxus has 3 NOS genes although evolutionary analysis has shown that there is no a direct relationship between the 3 amphioxus NOS and vertebrate eNOS, nNOS and iNOS genes (Andreakis et al., 2011). This indicates that, despite its high conservation, NOS evolution has also been very dynamic in some respects, with recurrent episodes of lineage-specific gene duplications. The study of amphioxus NOS will help to understand the acquisition of new functions of NOS enzymes, but also might illustrate convergent evolution events during NOS evolution. The following thesis project is designed to accomplish the first complete and detailed study of NOS during development. Although much has been published on NOS expression and function in many different organisms, very little is known about its role during the first stages of animal development

Mitochondrial genome sequencing of marine leukaemias reveals cancer contagion between clam species in the Seas of Southern Europe

20 Pág.Clonally transmissible cancers are tumour lineages that are transmitted between individuals via the transfer of living cancer cells. In marine bivalves, leukaemia-like transmissible cancers, called hemic neoplasia (HN), have demonstrated the ability to infect individuals from different species. We performed whole-genome sequencing in eight warty venus clams that were diagnosed with HN, from two sampling points located more than 1000 nautical miles away in the Atlantic Ocean and the Mediterranean Sea Coasts of Spain. Mitochondrial genome sequencing analysis from neoplastic animals revealed the coexistence of haplotypes from two different clam species. Phylogenies estimated from mitochondrial and nuclear markers confirmed this leukaemia originated in striped venus clams and later transmitted to clams of the species warty venus, in which it survives as a contagious cancer. The analysis of mitochondrial and nuclear gene sequences supports all studied tumours belong to a single neoplastic lineage that spreads in the Seas of Southern Europe.We thank the Galicia Supercomputing Centre (CESGA) for the availability of informatic resources. JMCT, SR, SD, and JT are supported by European Research Council (ERC) Starting Grant 716,290 SCUBA CANCERS. ALB is supported by MINECO PhD fellowship BES-2016-078166. DG-S is supported by postdoctoral contract ED481B/2018/091 from Xunta de Galicia. DP is supported by ERC grant ERC-617457-PHYLOCANCER and by Spanish Ministry of Economy and Competitiveness (MINECO) grant PID2019-106247GB-I00. This research was partially funded by the European Union’s Horizon 2020 research and innovation programme under grant agreement 730984, ASSEMBLE Plus project. CESAM got financial support from FCT/MEC (UIDP/50017/2020, UIDB/50017/2020).Peer reviewe

Rozvoj podnikání na Trhu s Drahými Kovy v Rusku

I have divided my work into 3 chapters.General information of precious metals.The purpose of my work is to analyze the possibility of expanding the sales markets of the company EZOCM.Also, consider one of the options, opening of the foreign manufacture, including countries of the consumers of the company EZOCM

Additional file 2: of Rab32 and Rab38 genes in chordate pigmentation: an evolutionary perspective

Database of sequences used in phylogeny of Fig.  1 . (TXT 14 kb

Expression Pattern of nos1 in the Developing Nervous System of Ray-Finned Fish

Fish have colonized nearly all aquatic niches, making them an invaluable resource to understand vertebrate adaptation and gene family evolution, including the evolution of complex neural networks and modulatory neurotransmitter pathways. Among ancient regulatory molecules, the gaseous messenger nitric oxide (NO) is involved in a wide range of biological processes. Because of its short half-life, the modulatory capability of NO is strictly related to the local activity of nitric oxide synthases (Nos), enzymes that synthesize NO from L-arginine, making the localization of Nos mRNAs a reliable indirect proxy for the location of NO action domains, targets, and effectors. Within the diversified actinopterygian nos paralogs, nos1 (alias nnos) is ubiquitously present as a single copy gene across the gnathostome lineage, making it an ideal candidate for comparative studies. To investigate variations in the NO system across ray-finned fish phylogeny, we compared nos1 expression patterns during the development of two well-established experimental teleosts (zebrafish and medaka) with an early branching holostean (spotted gar), an important evolutionary bridge between teleosts and tetrapods. Data reported here highlight both conserved expression domains and species-specific nos1 territories, confirming the ancestry of this signaling system and expanding the number of biological processes implicated in NO activities

Evolution of the nitric oxide synthase family in vertebrates and novel insights in gill development.

Nitric oxide (NO) is an ancestral key signalling molecule essential for life and has enormous versatility in biological systems, including cardiovascular homeostasis, neurotransmission and immunity. Although our knowledge of NO synthases (Nos), the enzymes that synthesize NO in vivo, is substantial, the origin of a large and diversified repertoire of nos gene orthologues in fishes with respect to tetrapods remains a puzzle. The recent identification of nos3 in the ray-finned fish spotted gar, which was considered lost in this lineage, changed this perspective. This finding prompted us to explore nos gene evolution, surveying vertebrate species representing key evolutionary nodes. This study provides noteworthy findings: first, nos2 experienced several lineage-specific gene duplications and losses. Second, nos3 was found to be lost independently in two different teleost lineages, Elopomorpha and Clupeocephala. Third, the expression of at least one nos paralogue in the gills of developing shark, bichir, sturgeon, and gar, but not in lamprey, suggests that nos expression in this organ may have arisen in the last common ancestor of gnathostomes. These results provide a framework for continuing research on nos genes' roles, highlighting subfunctionalization and reciprocal loss of function that occurred in different lineages during vertebrate genome duplications