314 research outputs found
Bacteriophage and their potential roles in the human oral cavity.
The human oral cavity provides the perfect portal of entry for viruses and bacteria in the environment to access new hosts. Hence, the oral cavity is one of the most densely populated habitats of the human body containing some 6 billion bacteria and potentially 35 times that many viruses. The role of these viral communities remains unclear; however, many are bacteriophage that may have active roles in shaping the ecology of oral bacterial communities. Other implications for the presence of such vast oral phage communities include accelerating the molecular diversity of their bacterial hosts as both host and phage mutate to gain evolutionary advantages. Additional roles include the acquisitions of new gene functions through lysogenic conversions that may provide selective advantages to host bacteria in response to antibiotics or other types of disturbances, and protection of the human host from invading pathogens by binding to and preventing pathogens from crossing oral mucosal barriers. Recent evidence suggests that phage may be more involved in periodontal diseases than were previously thought, as their compositions in the subgingival crevice in moderate to severe periodontitis are known to be significantly altered. However, it is unclear to what extent they contribute to dysbiosis or the transition of the microbial community into a state promoting oral disease. Bacteriophage communities are distinct in saliva compared to sub- and supragingival areas, suggesting that different oral biogeographic niches have unique phage ecology shaping their bacterial biota. In this review, we summarize what is known about phage communities in the oral cavity, the possible contributions of phage in shaping oral bacterial ecology, and the risks to public health oral phage may pose through their potential to spread antibiotic resistance gene functions to close contacts
Dye-Loaded Mechanochromic and pH-Responsive Elastomeric Opal Films
In this work, the preparation and fabrication of elastomeric opal films revealing reversible mechanochromic and pH-responsive features are reported. The core-interlayer-shell (CIS) particles are synthesized via stepwise emulsion polymerization leading to hard core (polystyrene), crosslinked interlayer (poly(methyl methacrylate-co-allyl methacrylate), and soft poly(ethyl acrylate-co-butyl acrylate-co-(2-hydroxyethyl) methacrylate) shell particles featuring a size of 294.9 ± 14.8 nm. This particle architecture enables the application of the melt-shear organization technique leading to elastomeric opal films with orange, respectively, green brilliant reflection colors dependent on the angle of view. Moreover, the hydroxyl moieties as part of the particle shell are advantageously used for subsequent thermally induced crosslinking reactions enabling the preparation of reversibly tunable mechanochromic structural colors based on Bragg's law of diffraction. Additionally, the CIS particles can be loaded upon extrusion or chemically by a postfunctionalization strategy with organic dyes implying pH-responsive features. This convenient protocol for preparing multi-responsive, reversibly stretch-tunable opal films is expected to enable a new material family for anti-counterfeiting applications based on external triggers
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Dye-Loaded Mechanochromic and pH-Responsive Elastomeric Opal Films
In this work, the preparation and fabrication of elastomeric opal films revealing reversible mechanochromic and pH-responsive features are reported. The core–interlayer–shell (CIS) particles are synthesized via stepwise emulsion polymerization leading to hard core (polystyrene), crosslinked interlayer (poly(methyl methacrylate-co-allyl methacrylate), and soft poly(ethyl acrylate-co-butyl acrylate-co-(2-hydroxyethyl) methacrylate) shell particles featuring a size of 294.9 ± 14.8 nm. This particle architecture enables the application of the melt-shear organization technique leading to elastomeric opal films with orange, respectively, green brilliant reflection colors dependent on the angle of view. Moreover, the hydroxyl moieties as part of the particle shell are advantageously used for subsequent thermally induced crosslinking reactions enabling the preparation of reversibly tunable mechanochromic structural colors based on Bragg's law of diffraction. Additionally, the CIS particles can be loaded upon extrusion or chemically by a postfunctionalization strategy with organic dyes implying pH-responsive features. This convenient protocol for preparing multi-responsive, reversibly stretch-tunable opal films is expected to enable a new material family for anti-counterfeiting applications based on external triggers
Immunopathological properties of the Campylobacter jejuni flagellins and the adhesin CadF as assessed in a clinical murine infection model
Background:
Campylobacter jejuni infections constitute serious threats to human health with increasing prevalences worldwide. Our knowledge regarding the molecular mechanisms underlying host-pathogen interactions is still limited. Our group has established a clinical C. jejuni infection model based on abiotic IL-10-/- mice mimicking key features of human campylobacteriosis. In order to further validate this model for unraveling pathogen-host interactions mounting in acute disease, we here surveyed the immunopathological features of the important C. jejuni virulence factors FlaA and FlaB and the major adhesin CadF (Campylobacter adhesin to fibronectin), which play a role in bacterial motility, protein secretion and adhesion, respectively.
Methods and results:
Therefore, abiotic IL-10-/- mice were perorally infected with C. jejuni strain 81-176 (WT) or with its isogenic flaA/B (ΔflaA/B) or cadF (ΔcadF) deletion mutants. Cultural analyses revealed that WT and ΔcadF but not ΔflaA/B bacteria stably colonized the stomach, duodenum and ileum, whereas all three strains were present in the colon at comparably high loads on day 6 post-infection. Remarkably, despite high colonic colonization densities, murine infection with the ΔflaA/B strain did not result in overt campylobacteriosis, whereas mice infected with ΔcadF or WT were suffering from acute enterocolitis at day 6 post-infection. These symptoms coincided with pronounced pro-inflammatory immune responses, not only in the intestinal tract, but also in other organs such as the liver and kidneys and were accompanied with systemic inflammatory responses as indicated by increased serum MCP-1 concentrations following C. jejuni ΔcadF or WT, but not ΔflaA/B strain infection.
Conclusion:
For the first time, our observations revealed that the C. jejuni flagellins A/B, but not adhesion mediated by CadF, are essential for inducing murine campylobacteriosis. Furthermore, the secondary abiotic IL-10-/- infection model has been proven suitable not only for detailed investigations of immunological aspects of campylobacteriosis, but also for differential analyses of the roles of distinct C. jejuni virulence factors in induction and progression of disease
Synthese maßgeschneiderter Partikel zur Kombination mit Präkursoren für die Herstellung poröser Architekturen und schaltbarer Opale
Im Rahmen dieser Arbeit konnte die Herstellung maßgeschneiderter, polymerbasierter CIS-Partikel als Porenbildner und für schaltbare Opale gezeigt werden. Dabei stellte die starved-feed Emulsionspolymerisation ein universelles Werkzeug dar, um monodisperse Partikel mit definiertem Durchmesser, sowie einem idealen Kern-Schale-Verhältnis und gezielt eingestellter Monomerzusammensetzung zu synthetisieren. Für die Entwicklung poröser Keramiken wurden polymere Partikel synthetisiert, die folgend zu nanokompositären Partikeln umgesetzt wurden. Dazu wurde die EP mit der ATRP und der Sol-Gel-Chemie kombiniert, um anorganische Präkursoren und präkeramische Polymere als Schalenmaterial zu etablieren. Durch thermischen Abbau der Kerne wurden einerseits poröse SiCN-Keramiken generiert, andererseits wurden poröse (gemischte) Metalloxide mit definierter Porenstruktur erzeugt, die vielversprechende Kandidaten für den Einsatz in Lithium-Ionen-Batterien darstellen. Im Bereich der schaltbaren Opale wurden Saat-Polymerisationen im starved-feed Modus genutzt, um monodisperse CIS-Partikel herzustellen. Diese wurden mittels Extrusion additiviert und durch das Schmelze-Scherverfahren zu Opalen geformt. So wurden photonische, mechanochrome Materialien hergestellt, die zugleich durch den Einbau von Farbstoffen potentiell als Sicherheitsmerkmale eingesetzt werden können. In einem weiteren Projekt konnte gezeigt werden, dass eine Interaktion von Opalgittern mit Fotosäuren möglich ist.Within the present work, the starved-feed emulsion polymerization was used to generate tailored polymer-based CIS-particles for the fabrication of stimuli-responsive, photonic opal films and for soft templating of porous materials. For this purpose, different colloids were successfully tailored and synthesized with respect to the intended application. For the fabrication of ordered porous materials, tailored CIS-particles were synthesized and further converted to nanocomposites using ATRP and sol-gel chemistry. As a result, inorganic precursors and preceramic polymers were established as shell materials for composite particles. Through thermal treatment, it was possible to generate porous SiCN ceramics as well as porous (mixed) metal oxides that are promising candidates for lithium-ion batteries. In the field of stimuli-responsive opal films, starved-feed seeded emulsion polymerization was used to generate monodisperse CIS-particle. After additivation and melt-shear organization, opal films were generated. Thus, photonic and mechanochromic dye-loaded materials were synthesized as potential candidates for anti-counterfeiting materials. On the other hand, the synergistic effect of crystalline opal structure and photo acids was shown
Combining Soft Polysilazanes with Melt-Shear Organization of Core–Shell Particles: On the Road to Polymer-Templated Porous Ceramics
The preparation of ordered macroporous SiCN ceramics has attracted significant interest and is an attractive area for various applications, e.g., in the fields of catalysis, gas adsorption, or membranes. Non-oxidic ceramics, such as SiCN, own a great stability based on the covalent bonds between the containing elements, which leads to interesting properties concerning resistance and stability at high temperature. Their peculiar properties have become more and more important for a manifold of applications, like catalysis or separation processes, at high temperatures. Within this work, a feasible approach for the preparation of ordered porous materials by taking advantage of polymer-derived ceramics is presented. To gain access to free-standing films consisting of porous ceramic materials, the combination of monodisperse organic polymer-based colloids with diameters of 130 nm and 180 nm featuring a processable preceramic polymer is essential. For this purpose, the tailored design of hybrid organic/inorganic particles featuring anchoring sites for a preceramic polymer in the soft shell material is developed. Moreover, polymer-based core particles are used as sacrificial template for the generation of pores, while the preceramic shell polymer can be converted to the ceramic matrix after thermal treatment. Two different routes for the polymer particles, which can be obtained by emulsion polymerization, are followed for covalently linking the preceramic polysilazane Durazane1800 (Merck, Germany): (i) Free radical polymerization and (ii) atom transfer radical polymerization (ATRP) conditions. These hybrid hard core/soft shell particles can be processed via the so-called melt-shear organization for the one-step preparation of free-standing particle films. A major advantage of this technique is the absence of any solvent or dispersion medium, enabling the core particles to merge into ordered particle stacks based on the soft preceramic shell. Subsequent ceramization of the colloidal crystal films leads to core particle degradation and transformation into porous ceramics with ceramic yields of 18–54%
Combining Soft Polysilazanes with Melt-Shear Organization of Core–Shell Particles: On the Road to Polymer-Templated Porous Ceramics
The preparation of ordered macroporous SiCN ceramics has attracted significant interest and is an attractive area for various applications, e.g., in the fields of catalysis, gas adsorption, or membranes. Non-oxidic ceramics, such as SiCN, own a great stability based on the covalent bonds between the containing elements, which leads to interesting properties concerning resistance and stability at high temperature. Their peculiar properties have become more and more important for a manifold of applications, like catalysis or separation processes, at high temperatures. Within this work, a feasible approach for the preparation of ordered porous materials by taking advantage of polymer-derived ceramics is presented. To gain access to free-standing films consisting of porous ceramic materials, the combination of monodisperse organic polymer-based colloids with diameters of 130 nm and 180 nm featuring a processable preceramic polymer is essential. For this purpose, the tailored design of hybrid organic/inorganic particles featuring anchoring sites for a preceramic polymer in the soft shell material is developed. Moreover, polymer-based core particles are used as sacrificial template for the generation of pores, while the preceramic shell polymer can be converted to the ceramic matrix after thermal treatment. Two different routes for the polymer particles, which can be obtained by emulsion polymerization, are followed for covalently linking the preceramic polysilazane Durazane1800 (Merck, Germany): (i) Free radical polymerization and (ii) atom transfer radical polymerization (ATRP) conditions. These hybrid hard core/soft shell particles can be processed via the so-called melt-shear organization for the one-step preparation of free-standing particle films. A major advantage of this technique is the absence of any solvent or dispersion medium, enabling the core particles to merge into ordered particle stacks based on the soft preceramic shell. Subsequent ceramization of the colloidal crystal films leads to core particle degradation and transformation into porous ceramics with ceramic yields of 18–54%
Embedding Photoacids into Polymer Opal Structures: Synergistic Effects on Optical and Stimuli-Responsive Features
Opal films with their vivid structural colors represent a field of tremendous interest
and obtained materials offer the possibility for many applications, such as optical sensors or anti counterfeiting materials. A convenient method for the generation of opal structures relies on the
tailored design of core-interlayer-shell (CIS) particles. Within the present study, elastomeric opal
films were combined with stimuli-responsive photoacids to further influence the optical properties
of structurally colored materials. Starting from cross-linked polystyrene (PS) core particles featuring
a hydroxy-rich and polar soft shell, opal films were prepared by application of the melt-shear
organization technique. The photoacid tris(2,2,2-trifluoroethyl) 8-hydroxypyrene-1,3,6-trisulfonate
(TFEHTS) could be conveniently incorporated during freeze-drying the particle dispersion and
prior to the melt-shear organization. Furthermore, the polar opal matrix featuring hydroxylic
moieties enabled excited-state proton transfer (ESPT), which is proved by spectroscopic evaluation.
Finally, the influence of the photoacid on the optical properties of the 3-dimensional colloidal
crystals were investigated within different experimental conditions. The angle dependence of the
emission spectra unambiguously shows the selective suppression of the photoacid’s fluorescence in
its deprotonated state
Maple River dam removal project.
LimnologyGiven the lack of empirical studies on dam systems before and after dam removal, our study aims to fill this gap by researching the physical and biological compositions of the East, West, and Main Branches of the Maple River. By examining sites at varying distances upstream and downstream of the Maple River dam, we are creating an inventory of pre-dam removal conditions that will serve as a reference for ecological scientists, dam owners, sportsmen, and local municipalities involved in the processes and potential outcomes of dam removal. Our data includes discharge rates, temperature, conductivity, functional feeding groups and aquatic organisms. These data inform our predictions about potential ecological impacts of the Maple River dam removal.http://deepblue.lib.umich.edu/bitstream/2027.42/116616/1/Boehm_Carey_Fromm_Gadway_McGlashen_MacNeille_Michaelson_2015.pd
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