Kreatywność we współczesnej sztuce pop . Inspiracje twórczością Warhola w edukacji studentów kierunków artystycznych

Pop-art was one of the most important artistic phenomena in the decade 1960-1970. In 2017 there was a thirty anniversary of andy Warhol’s death. This art revolutionist was a „painter of modern times”. He created, using mass culture products and at the same time showing the way the civilization is heading. a return to his creativeness in the international art project Warhol. pop contexts (30 years later) seems to be interesting: it presents students of art universities being representatives of the information generation who are inspired by Warhol’s work and also the direction in which the main aesthetics of pop style is currently migrating. young artists – are they creative or imitative? The article also describes educational dimension of the project.Pop-art był jednym z najważniejszych zjawisk artystycznych w dziesięcioleciu 1960–1970. W 2017 roku minęła trzydziesta rocznica śmierci andy’ego Warhola. Ów rewolucjonista sztuki był „malarzem współczesności”. Tworzył, posługując się produktami kultury masowej, przy okazji ukazując drogę, po jakiej zmierza cywilizacja. Powrót do jego twórczości w Międzynarodowym projekcie artystycznym Warhol. Pop konteksty (30 lat później) wydaje się ciekawy: jak studenci szkół artystycznych, będący przedstawicielami pokolenia informacyjnego, inspirują się twórczością Warhola i w jakim kierunku obecnie migruje główna estetyka stylu pop? Czy młodzi twórcy są kreatywni czy naśladowczy? Artykuł opisuje także edukacyjny wymiar projektu

Finansowe uwarunkowania decyzji ekonomicznych

Praca recenzowana / peer-reviewed pape

The effect of monochromatic light on the accumulation of phenolic acids and flavonoids in in vitro cultures of Aronia sp.

W ramach pracy badano akumulacje kwasów fenolowych i flawonoidów w kulturach pędowych trzech gatunków rodzaju Aronia (Aronia melanocarpa, Aronia arbutifolia i Aronia prunifolia) rosnących w obecności promieniowania UV-A, światła niebieskiego, światła czerwonego, w podczerwieni, w ciemności oraz w obecności światła białego (warunki kontrolne). Kultury prowadzono na podłożu Murashige’a i Skooga zawierającym 1 mg/l BAP i 1 mg/l NAA. W ekstraktach z biomasy z kultur in vitro zebranej po 4-tygodniowych cyklach hodowlanych (trzy serie) oznaczano metodą HPLC zawartość wolnych i związanych kwasów fenolowych oraz flawonoidów. Najwyższe całkowite zawartości analizowanych metabolitów zarówno kwasów fenolowych wolnych i związanych oraz flawonoidów uzyskano w ekstraktach z biomasy hodowanej w świetle niebieskim.¬Spośród dziewiętnastu analizowanych kwasów fenolowych i kwasu cynamonowego stwierdzono zawartość czterech wolnych kwasów fenolowych: kwasu chlorogenowego, kwasu neochlorogenowego, kwasu protokatechowego i kwasu rozmarynowego (ekstrakty z biomasy A. arbutifolia zawierała dodatkowo kwas 3,4-dihydroksyfenylooctowy). Po kwaśnej hydrolizie stwierdzono jakościowo inny skład kwasów fenolowych – pięć związanych kwasów fenolowych: kwas p-hydroksybenzoesowy, kwas kawowy, kwas protokatechowy, kwas syryngowy i kwas wanilinowy (ekstrakty z biomasy A. melanocarpa zawierała dodatkowo kwas 3,4-dihydroksyfenylooctowy). Spośród trzynastu flawonoidów biomasa kultur in vitro każdego analizowanego gatunku akumulowała cztery flawonoidy: cynarozyd, hyperozyd, kwercytryna i rutozyd. Dominującymi pod względem ilościowym metabolitami były depsydy, ich maksymalne zawartości stwierdzono w biomasie A. prunifolia: 418,83 mg/100 g s.m.(kwas chlorogenowy), 644,68 mg/100 g s.m. (kwas neochlorogenowy) i 548,86 mg/100 g s.m. (kwas rozmarynowy). W tej samej biomasie stwierdzono także najwyższą zawartość dominującego flawonoidu – kwercytryny (165,88 mg/100 g s.m.).Uzyskane wyniki wskazują kultury in vitro gatunków rodzaju Aronia, szczególnie kultury A. prunifolia hodowane w świetle niebieskim jako potencjalne źródło cennych kwasów fenolowych (w szczególności depsydów) oraz flawonoidów.The studies aimed to investigate the accumulation of phenolic acids and flavonoids in shoot cultures of three Aronia specis (Aronia melanocarpa, Aronia arbutifolia and Aronia prunifolia) cultivated under UV-A radiation, blue, red and infrared light, in the dark and in the presence of white light (control conditions). Cultures were maintained on the Murashige and Skoog medium supplemented with 1 mg/l BAP and 1 mg/l NAA. Extracts of biomass from in vitro cultures collected after 4-week growth cycles (three series) were used for determination of the content of free and bound phenolic acids and flavonoids using an HPLC method.The highest total contents of the analyzed metabolites, both free and bound phenolic acids and flavonoids were obtained in extracts from biomass cultured under blue light.Of nineteen analyzed phenolic acids and cinnamic acid, four free phenolic acids were identified in the biomass: chlorogenic acid, neochlorogenic acid, protocatechuic acid and rosmarinic acid (A. arbutifolia biomass extracts contained also 3,4-dihydroxyphenylacetic acid). The composition of phenolic acid after acid hydrolysis was different, namely the biomass contained five bound phenolic acids: p-hydroxybenzoic acid, caffeic acid, protocatechuic acid, syringic acid and vanillic acid (A. melanocarpa biomass extracts were found to contain also 3,4-dihydroxyphenylacetic acid). Of thirteen analyzed flavonoids, biomass from in vitro cultures of every tested species accumulated four flavonoids: cynaroside, hyperoside, quercetin and rutoside. The depsides were found to be the dominant metabolites and their maximum concentrations were determined in the biomass of A. prunifolia: 418.83 mg/100 g d.w.(chlorogenic acid), 644.68 mg/100 g d.w. (neochlorogenic acid) and 548.86 mg/100 g d.w. (rosmarinic acid). This biomass contained also the highest amount of the dominant flavonoid, i.e. quercetin (165.88 mg/100 g d.w.).The obtained results indicate that in vitro cultures of different species of the genus Aronia, especially A. prunifolia cultures maintained under blue light are a potential source of valuable phenolic acids (particularly depsides) and flavonoids

Sprawozdanie z uroczystości jubileuszu pięćdziesięciolecia Instytutu Nauk Administracyjnych Wydziału Prawa, Administracji i Ekonomii Uniwersytetu Wrocławskiego — „Niepomijalność administracji publicznej” (Wrocław, 7 czerwca 2019 roku)

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The Role of Adrenomedullin Signalling in Pancreatic Cancer

Pancreatic cancer (PaCa) is the 4th most common cause of cancer related death and incidence rates are increasing. Understanding how PaCa develops is important for earlier detection and better treatment outcomes. ADM is a peptide hormone associated with many cancers. It has physiological and pathological roles regulated through receptor complexes composed of CLR and RAMPs. RAMPs alter the selectivity of receptor, the AM1 receptor (CLR and RAMP-2) is associated with physiological roles (regulating blood pressure), the AM2 receptor (CLR and RAMP-3) is associated with pathological roles. The AM2 receptor has been shown to regulate tumour growth and survival, angiogenesis, immunosuppression and epithelial-to-mesenchymal transition. The aim of this study was the elucidate the role of ADM in PaCa. The results showed that in a panel of seven PaCa cell lines, ADM, CLR and RAMPs were expressed at both mRNA and protein level. Therefore, inducible CFPAC-1 ADM KDs, CFPAC-1 RAMP-3 KDs and scrshRNA controls were developed and used for in vitro and in vivo experiments. In vitro, viability and apoptosis assays showed that both KD and control cells responded to gemcitabine treatment but not to 5-fluorouracil. In vivo, both orthotopic and subcutaneous models showed no significant difference in tumour weight or volume between different groups (CFPAC-1 ADM KD, scrshRNA and wild-type cells). There were no significant differences between the percentage of immune cells in different groups. Furthermore, immunohistochemistry showed no significant differences in the number of Ki67 positive cells. Analysis of α-SMA and endomucin needed further work. Overall, the results demonstrate that ADM and its receptor components are expressed in PaCa cell lines. However, further elucidation of the exact role of ADM in tumour development is needed with more focus on the tumour stroma. Focus on pancreatic stellate cells (α-SMA) shows promise in the field of PaCa

Microstructure-properties relation of hydrostatically extruded absorbable zinc alloys:Effect of Mg and Cu addition on corrosion properties and biocompatibility

Pure Zn is well-known for its appropriate corrosion rate, making it suitable for use as future absorbable implants. Yet, it suffers from insufficient strength, thus, both plastic deformation and alloying are required. Hydrostatic extrusion has proven to be an efficient technique, providing high mechanical properties for zinc alloys. However, its effect on degradation rate and biocompatibility of Zn alloys remains unknown. Thus, within the present study, an attempt to evaluate those properties has been made on hydrostatically extruded pure Zn, Zn–Mg and Zn–Mg–Cu alloys. The materials were characterized by advanced microscopy techniques and uniaxial tensile tests. Corrosion properties were assessed based on electrochemical and static immersion tests. Finally, the cytotoxic effect of zinc extracts on endothelial cells were examined by standard MTT assays combined with confocal imaging. The results showed that hydrostatic extrusion results in significant refinement of α-Zn grains and the intermetallic phase Mg2Zn11 for the investigated alloys. The alloys exhibited ultimate tensile strength exceeding 300 MPa and elongation higher than 20%. Corrosion tests demonstrated that all the materials showed a similar level of degradation rate. Moreover, the uniform distribution of the intermetallic phase contributed to homogeneous corrosion of Zn alloys. Biological studies indicated that the least cytotoxic response in endothelial cells was obtained for the Zn–Mg alloy. Such an effect was caused by the limited amount of released Zn ions in the favor of Mg ions. The refinement of α-Zn grains and intermetallic phases caused by hydrostatic extrusion were key factors determining the performance of Zn-based materials.</p