PERBANDINGAN EFEKTIFITAS PEMBELAJARAN DARING DAN LURING PADA MATA PELAJARAN DESAIN BUSANA

Adanya pandemi COVID-19 yang telah diumumkan oleh WHO berdampak pada dunia Pendidikan. Hal ini dikarenakan pemerintah menghimbau lembaga Pendidikan untuk meniadakan pembelajaran secara tatap muka atau luring untuk memutus penularan COVID-19, serta menganjurkan diberlakukannya pembelajaran secara daring atau online. Perubahan ini berimbas pada proses pembelajaran, dimana sebelumnya guru dan siswa terbiasa melakukan pembelajaran tatap muka dan seketika berubah menjadi daring sehingga memerlukan adaptasi bagi guru dan siswa. Adaptasi ini akan berpengaruh pada efektivitas pembelajaran untuk mencapai tujuan pembelajaran. Tujuan penelitian ini yaitu: 1) Mengetahui perbedaan hasil pembelajaran daring dan luring pada mata pelajaran Desain Busana, 2) Mengetahui efektifitas media pembelajaran daring dan luring pada mata pelajaran Desain Busana. Penelitian ini menggunakan metode Literature Review dengan mengkaji penelitian yang sesuai. Ditemukan 5 atikel pembelajaran daring dan 5 artikel pembelajaran luring. Hasil dari penelitian ini yaitu: 1) Pembelajaran luring mendapatkan 87,25% dengan klasifikasi sangat baik dan pembelajaran daring mendapatkan hasil 83,33% dengan klasifikasi sangat baik. Sehingga dapat disimpulkan bahwa pembelajaran luring memberikan hasil belajar yang lebih baik dibanding pembelajaran daring pada mata pelajaran Desain Busana, 2) Media pembelajaran luring  mendapatkan hasil 82,73%  dengan klasifikasi sangat baik dan media pembelajaran daring mendapatkan hasil 81,85% dengan klasifikasi sangat baik, dapat disimpulkan bahwa media pembelajaran luring lebih baik dibandingkan media pembelajaran darin

UJI IN VITRO CARBONATE APATITE-CHITOSAN SCAFFOLDS SEBAGAI MATERIAL CANGKOK TULANG PADA TEKNIK REKAYASA JARINGAN: IN VITRO TEST OF APATITE - CHITOSAN CARBONATE SCAFFOLDS AS BONE GRAFT MATERIAL IN TISSUE ENGINEERING TECHNIQUES

Untuk meningkatkan adhesi sel dan kemampuan pembentukan tulang dari kitosan, dicoba untuk membuat suatuscaffolds yang menggabungkan kitosan dengan carbonate apatite (CA). Tujuan penelitian ini adalah untukmembuat carbonate apatite-chitosan scaffolds (CA-ChSs) serta mengevaluasi CA-ChSs dari sudut pandangproliferasi sel menggunakan MC3T3-E1. Chitosan scaffolds (ChSs) yang berisi 25, 50, 75, 100, 125, 150, 200dan 400 mg bubuk kitosan (100D, YSK, Japan) dibuat dengan prosedur dilarutkan dalam 5 ml asam asetat 2%,dikocok selama 15 menit, kemudian dinetralkan dengan 15 ml 0,1 M larutan NaOH. Setelah disentrifugasi pada1500 rpm selama 10 menit, kelebihan air dibuang, kemudian gel kitosan dikemas ke dalam cetakan teflon(diameter: 5 mm, tinggi: 2 mm). Cetakan dibekukan pada suhu -80°C selama 2 jam dan dikeringkan dalammesin beku kering pada suhu -54°C selama 24 jam. Selanjutnya dilakukan radiasi ultraviolet pada ChSs selama 2jam. Untuk membuat CA-ChSs, dipilih ChSs yang berisi 200 mg bubuk kitosan. Setelah dinetralisir, 10, 50, 100,200 dan 300 mg dari 0,06 M CA ditambahkan ke dalam kitosan gel yang berisi 200 mg bubuk kitosan. StrukturCA-ChSs diamati dengan scanning electron microscope (SEM). Proliferasi MC3T3-E1 dalam ChSs dan CAChSs dievaluasi pada hari ke-1, 7 dan 14. CA-ChSs dengan struktur tiga dimensi yang berpori serta adanya perlekatan CA dapat diamati dengan jelas menggunakan SEM. Hasil penelitian menunjukkan jumlahpertumbuhan dan perkembangan sel pada CA-ChSs secara signifikan lebih banyak dibandingkan pada ChSs(kontrol) pada setiap tahap pengamatan di hari ke-1, 7 dan 14 (p< 0,05). Sebagai kesimpulan, CA-ChSs adalahkandidat untuk material cangkok tulang pada teknik rekayasa jaringan

Effect of Home Bleaching Agents on The Surface Hardness Of Flowable Bulk Fill Resin Composite

Composite resins have disadvantages such as low hardness level, shrinkage, and micro-leakage. In the case of deep cavity restorations, bulk-fill composite resins have begun to be used because they facilitate the restoration work and make the restoration time shorter. Purpose: This study was used to determine whether home bleaching treatment using hydrograph peroxide and carbamide peroxide can reduce the surface hardness of flowable bulk-fill composite resins. Methods: The research sample is cylindrical with a diameter of 5 mm and a height of 2 mm, this study was divided into 3 groups. Then each group was measured by Vickers Hardness Tester. Results: In the One Way Anova test the value of p = 0.020 was obtained. This indicates a significant difference in the surface hardness of bulk-fill flowable composite resins and a Post hoc test - Tukey HSD must be performed. Conclusion: The results show that hydrogen peroxide home bleaching agent can significantly reduce the surface hardness of bulk-fill flowable composite resin

Antioxidant Effects of Graptophyllum pictum Leaf Extract on Malondialdehyde (MDA) Levels of Mice Induced By a Toxic Dose of Paracetamol

Background: Antioxidants are important substances which possess the ability to protect the body from damage caused by free radicals-induced oxidative stress. A shift in the balance between oxidants and antioxidants promotes oxidative stress. Graptophyllum pictum (GP) contains substances that are efficacious as antioxidants. Aim and Objectives:To prove the antioxidant effects of GP leaf extract on Malondialdehyde (MDA) levels of mice induced by a toxic dose of paracetamol. Material and Methods: Thirty mice were randomly divided into five groups(n=6); Negative Control (NC) was without treatment, Positive Control (PC) with aquadest + paracetamol, T1: GP leaf extract 150 mg/kg BW + paracetamol, T2: GP leaf extract 300 mg/kg BW+ paracetamol, T3: GP leaf extract 600 mg/kg BW+ paracetamol. Extracts were given on the 1st to 10th day and paracetamol induction was performed on the 8th, 9th, and 10th days. On the 11th day, blood serum samples were collected and the level of MDA was then measured Results: T1, T2, T3 had the lower number of MDA levels when compared with the PC (3.625±0.374, 3.147±0.222, 2.574±0.319). One way Analysis of Variance (ANOVA) and post hoc test with Tukey showed that there was a significant difference between PC and T1, T2, T3 (p<0.05). Conclusion: GP extract has an antioxidant effect on the prevention of elevated MDA levels. A dose of 600 mg/kg BW was found to be the most effective in preventing elevation of MDA levels after a toxic dose of paracetamol was induced

Proliferation and osteogenic differentiation of bone marrow‑derived mesenchymal stem cell after exposure to red flesh dragon fruit extract

Background: Induction of the proliferation and differentiation of stem cells could represent a viable alternative therapeutic method for treating bone diseases. Stem cells are essential to bone tissue regeneration; although, their availability is limited. One possible method of increasing the number of stem cells and promote osteogenic differentiation is the application of red flesh dragon fruit extract supplement. The present study was performed to identify and analyze proliferation and osteogenic differentiation of bone marrow‑derived mesenchymal stem cells (BMMSCs) after exposure to red flesh dragon fruit extract. Materials and Methods: This in vitro study was posttest only control group design. Red flesh dragon fruit extract was produced by means of water extraction method and subsequent dilution with different amounts of water to produce a range of concentrations. BMMSCs were obtained from the femurs of three White New Zealand rabbits. BMMSCs were then treated with 50, 100, 200, 300, and 400 μg/ml red flesh dragon fruit extract concentrations. The in vitro proliferation assay was determined by means of an ‑(4.5‑dimethylthiazol‑2‑yl)‑2,5‑diphenyltetrazolium bromide assay. Osteogenic differentiation was determined by means of the degree of nodule mineralization. There were two groups as follows: group I with the addition of 50 μg/ml of red flesh dragon fruit extract and Group II without the addition of red flesh dragon fruit. Data were analyzed using analysis of variance and the Student’s t‑test (P = 0.05). Results: 50, 100, 200, 300, and 400 μg/ml of red flesh dragon fruit extract demonstrated the capacity to significantly increase the proliferation of BMMSCs (P ≤ 0.05). Red flesh dragon fruit extract could significantly increase osteogenic differentiation (P ≤ 0.05). Conclusion: Red flesh dragon fruit extract enhances proliferation and osteogenic differentiation of BMMSCs

Compressive strength and porosity tests on bovine hydroxyapatitegelatin-chitosan scaffolds

Background: Degenerative diseases, aggressive Periodontitis, trauma, jaw resection, and congenital abnormalities can cause defects in jaw bone. The surgical procedure for bone reconstruction currently performed is bone regeneration graft (BRG). Unfortunately, this procedure still has many disadvantages. Thus, tissue engineering approach is necessary to be conducted. The main component used in this tissue engineering is scaffolds. Scaffolds used in bone regeneration is expected to have appropriate characteristics with bone, such as high porosity and swelling ratio, low degradation rates, and good mechanical properties. For those reasons, this research used scaffolds made from bovine hydroxyapatite (BHA), gelatin (GEL), and chitosan (K)/BHA-GEL-K as one of biomaterial candidates for bone regeneration. Purpose: This study aimed to determine compressive strength value and porosity size of BHA-GEL-K scaffolds. Method: Compressive strength of BHA-GEL-K scaffolds was tested using autograph with speed 10 mm/ min with a load cell compress machine of 100 kN. Compressive strength was calculated by force divided to surface area. Porosity test was measured using SEM. Scaffold were coated with Pb and Au, then the porosity size is calculated with SEM at 100x magnification. Result: BHA-GEL-K scaffolds had a mean compressive strength value of 174.29 kPa and a porosity size of 31.62 + 147.06 lm. Conclusion: It can be concluded that BHA-GEL-K scaffolds has a good compressive strength, but not yet resemble real bone mass, while porosity of BHA-GEL-K scaffold is appropriate for bone tissue regeneration application

Cytotoxicity Test of Sponge Amnion on BHK-21 Fibroblast Cell

Biomaterials that can be used to accelerate wound healing is the amniotic membrane that contains growth hormone. In its use, amniotic membrane tends to be difficult to use because of their sheets form. To make it easier to use, the amniotic membrane is mixed with gelatin as an dhesive material to produce sponge amnion. Sponge amnion which is used as a biomaterial needs to meet several requirements, one of them is biocompatibility. To complete these requirements cytotoxicity testing is necessary to termine the toxic potential that a material may produce. The cytotoxicity test was performed on BHK-21 fibroblast cells. The aim of this study is to determine the litotoxicity effect of amnion in sponge form on BHK-21 fibroblast cells. This study was done towards the culture cell line BHK-21 using MTT assay method. In this study used four sample groups. Two groups are the control group (media control and cell control), and the other two groups are samples consisted of amniotic membrane and sponge amnion. Living cells were quantified after Treatment by ELISA reader on 620 nm. The fibroblast cell life percentage after exposure with amniotic membrane and sponge amnion is 98,16% and 98.13%. Based on the ANOVA test result was obtained P-value of 0.000 (P-value <0.05) so that it can be said that the treatment result gives a significant difference. Amnion in sponge form has no toxic for BHK-21 fibroblast cell

PENERAPAN SISTEM KETERTELUSURAN (TRACEABILITY) PADA PRODUK UDANG VANNAMEI BREADED BEKU (FROZEN BREADED SHRIMP) DI PT. RED RIBBON JAKARTA

Penelitian ini bertujuan untuk mengetahui alur proses pengolahan udang breaded beku, sistem traceability pengolahan udang breaded beku mulai dari proses penerimaan bahan baku, pengolahan sampai produk akhir serta mutu bahan baku dan produk akhir melalui uji organoleptik dan mikrobiologi. Metode kerja dilakukan dengan mengamati langsung diperusahaan melalui pengambilan data suhu produk setiap alur proses dan pemantauan penerapan ketertelusuran diperusahaan. Hasil penelitian menunjukkan bahwa penerapan ketertelusuran Internal di PT. Red Ribbon sudah diterapkan selama proses penerimaan dibahan baku sampai produk akhir. Ketertelusuran eksternal perusahaan menggunakan sistem tertutup, yang mana pemasok bertanggung jawab dalam memberikan informasi kepada pihak perusahaan atas produk yang dikirim. Hasil Uji organoleptik bahan baku dan produk akhir sesuai standar SNI yaitu 7. Hasil uji ALT, E.coli dan coliform masih memenuhi standar SNI yaitu 5 x105 untuk bahan baku dan 2 x 105 untuk produk akhir pada ALT dan &lt;2 MPN/gr pada E.coli dan coliform

Vascular Endothelial Growth Factor Expression after Induced by Chicken Shank Collagen Scaffold in Bone Regeneration

Vascular endothelial growth factor (VEGF) is an essential mediator during the process of angiogenesis that is an initial process of bone regeneration. Engineered bone tissue commonly encompass the use of scaffold. Collagen plays an important role in bone regeneration. Unfortunately, collagen can be extracted from chicken shank. This research was aimed to determine the effect of chicken shank collagen scaffold on the expresion of VEGF. Chicken shank was mixed by trypsin powder and dissolved in CH3COOH until forming like fiber then was sentifuged. Supernatant was dissolved NaCl 5wt% until forming fiber then was dialyzed for three days. Chicken shank collagen was printed in mould to freeze dry for 24 hours. Rats with defected femur were divided into 2 groups. Group 1 was the control group, and group 2 was treatment group which the defected femur were administrated with chicken shank collagen scaffold. Those rats were sacrificed on 2nd week. Tissue preparation was made then immunohistochemical staining was conducted counting VEGF expression. A statistical analysis was conducted using Mann Whitney test. There was a significant difference in the expression of VEGF (p<0,05). The amount of VEGF expression was increased by chicken shank collagen scaffold