Dried Volumetric Microsampling Approaches for the Therapeutic Drug Monitoring of Psychiatric Patients Undergoing Clozapine Treatment

Clozapine is one of the most widely used second-generation antipsychotic drugs (SGAs) for the treatment of schizophrenia. Despite advantages over first-generation drugs, clozapine still shows significant side effects and interindividual variations in efficacy. In order to ensure frequent therapeutic drug monitoring (TDM) and improve the compliance of psychiatric patients undergoing clozapine treatment, two novel dried microsampling approaches based on whole blood and plasma volumetric absorptive microsampling (b-VAMS and p-VAMS) and microfluidic generated-dried blood spot technology (mfDBS) were developed and coupled to HPLC with electrochemical detection (ED). The proposed miniaturized strategies by means of VAMS and microfluidic channel-based devices provide several advantages in terms of collection, storage, and handling compared to classical blood and plasma processing. Satisfactory validation results were obtained for all microsampling platforms, with mean extraction yields >85.1%, precision as relative standard deviation (RSD) < 5.1%, and stability < 4.5% analyte loss after 30 days for p-VAMS; mean extraction yields > 83.4%, precision RSD < 5.4%, and stability < 4.6% analyte loss after 30 days for b-VAMS, and mean extraction yields > 74.0%, precision RSD < 5.6%, and stability < 4.9% analyte loss after 30 days for mfDBS. The original microsampling methodologies have been successfully applied to the blood and plasma collected from five psychiatric patients for the monitoring of the levels of clozapine and its main metabolites, providing robust and reliable quali-quantitative results. Comparisons between results of the two dried microsampling technologies with those obtained by classic fluid plasma analysis were in good agreement and have demonstrated that the proposed miniaturized approaches could be suitable for TDM purposes

Superconducting circuit optomechanics in topological lattices

Cavity optomechanics enables controlling mechanical motion via radiation pressure interaction, and has contributed to the quantum control of engineered mechanical systems ranging from kg scale LIGO mirrors to nano-mechanical systems, enabling ground-state preparation, entanglement, squeezing of mechanical objects, position measurements at the standard quantum limit, non-reciprocal photon transport, and quantum transduction. Yet, nearly all prior schemes have employed single- or few-mode op-tomechanical systems. In contrast, novel dynamics and applications are expected when utilizing optomechanical arrays and lattices, which enable to synthesize non-trivial band structures, and have been actively studied in the field of circuit QED. Superconducting microwave optomechanical circuits are a promising platform to implement such lattices, but have been compounded by strict scaling limitations. Here, we overcome this challenge and realize superconducting circuit optomechanical lattices. We demonstrate non-trivial topological microwave modes in 1D optomechanical chains realizing the canonical Su-Schrieffer-Heeger (SSH) model. Furthermore, we realize the strained graphene model in a 2D optomechanical honeycomb lattice. Exploiting the embedded optomechanical interaction, we show that it is possible to directly measure the mode functions of the bulk modes, as well as the topologically protected edge states, without using any local probe or inducing perturbation. This enables us to reconstruct the full underlying lattice Hamiltonian. Such optomechanical lattices, accompanied by the measurement techniques introduced, offers an avenue to explore out of equilibrium physics in optomechanical lattices such as collective, quantum many-body, and quench dynamics, topological properties and more broadly, emergent nonlinear dynamics in complex optomechanical systems with a large number of degrees of freedoms.Comment: Updated version with a comprehensive discussion on strained graphene mode

MAGIC and H.E.S.S. detect VHE gamma rays from the blazar OT081 for the first time: a deep multiwavelength study

https://pos.sissa.it/395/815/pdfPublished versio

The air hunger response of four elite breath-hold divers

Photograph of the CO-NM-OK Tri-State Marker