Histological description of the reproductive tract of male and female hatchlings of the magdalena river turtle (podocnemis lewyana).

In this study we review the macro and microscopic description of the reproductive system of male and female Podocnemis lewyana neonates of 3.5 months of age. We found macroscopic differences in the morphology of the gonads, with ovaries being twice longer that testes, and testes being twice wider than ovaries. Microscopically we identified several immature elements, such as the lack of muscle layer in the oviduct of reptile females, and simple epithelia instead of pseudostratified epithelia in the oviduct and epididymis described in adults. We also describe a black pigment observed macroscopically in the mesovarium and macroscopically and histologically in the epididymis. This pigment is compatible with the center of melano-macrophages described in other vertebrates. Finally we described a supporting mesenchymal structure, the appendage of the oviduct, with was much longer than what has been described in other Podocnemis species

Histological description of the reproductive tract of male and female hatchlings of the magdalena river turtle (podocnemis lewyana).

In this study we review the macro and microscopic description of the reproductive system of male and female Podocnemis lewyana neonates of 3.5 months of age. We found macroscopic differences in the morphology of the gonads, with ovaries being twice longer that testes, and testes being twice wider than ovaries. Microscopically we identified several immature elements, such as the lack of muscle layer in the oviduct of reptile females, and simple epithelia instead of pseudostratified epithelia in the oviduct and epididymis described in adults. We also describe a black pigment observed macroscopically in the mesovarium and macroscopically and histologically in the epididymis. This pigment is compatible with the center of melano-macrophages described in other vertebrates. Finally we described a supporting mesenchymal structure, the appendage of the oviduct, with was much longer than what has been described in other Podocnemis species

La vida cotidiana ante el COVID-19: Modos diferenciales de usar y valorar el espacio en el Gran Buenos Aires durante la fase 1 del ASPO, 2020

La llegada del COVID-19 a la Argentina y la política de Aislamiento Social Preventivo y Obligatorio (ASPO) transformaron los usos y valoraciones en torno al espacio público y privado. Este artículo focaliza en la dimensión espacial de la política sanitaria con el objetivo de analizar cómo las/os habitantes del Gran Buenos Aires (GBA) modificaron su relación con el espacio urbano y el espacio doméstico a partir de la medida del ASPO. Sobre la base de una encuesta online realizada durante las primeras semanas de la Fase 1 de ASPO a residentes del GBA mayores de 18 años con secundario completo o más, se indaga cómo el género, la composición del hogar, la zona geográfica de residencia y la disponibilidad de espacios exteriores en la vivienda inciden en los modos de usar y valorar el espacio público y privado. Entre los resultados, se destaca que la pandemia profundizó desigualdades preexistentes referidas tanto a los usos del espacio doméstico como a las formas de valorar el espacio público, de modo tal que son las mujeres quienes experimentan con mayores cargas laborales y emocionales este período del ASPO.Fil: Marcus, Juliana. Universidad de Buenos Aires. Facultad de Ciencias Sociales. Instituto de Investigaciones "Gino Germani"; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Boy, Martín Guillermo. Universidad Nacional de Jose Clemente Paz; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Sociales. Instituto de Investigaciones "Gino Germani"; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Benitez, Joaquín Andrés. Universidad Nacional de General Sarmiento; Argentina. Universidad de Buenos Aires; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Berardo, Martina Daniela. Universidad Nacional de General Sarmiento; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Sociales. Instituto de Investigaciones "Gino Germani"; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Felice, Magdalena Inés. Universidad de Buenos Aires. Facultad de Ciencias Sociales. Instituto de Investigaciones "Gino Germani"; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Marquez, Agustina. Universidad de Buenos Aires. Facultad de Ciencias Sociales. Instituto de Investigaciones "Gino Germani"; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Peralta, María Agustina. Universidad de Buenos Aires; ArgentinaFil: Vazquez, Diego Ezequiel. Universidad de Buenos Aires. Facultad de Ciencias Sociales. Instituto de Investigaciones "Gino Germani"; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentin

HNRNPDL-related muscular dystrophy: expanding the clinical, morphological and MRI phenotypes

Autosomal dominant limb girdle muscular dystrophy D3 HNRNPDL-related is a rare dominant myopathy caused by mutations in HNRNPDL. Only three unrelated families have been described worldwide, a Brazilian and a Chinese carrying the mutation c.1132G>A p.(Asp378Asn), and one Uruguayan with the mutation c.1132G>C p. (Asp378His), both mutations occurring in the same codon. The present study enlarges the clinical, morphological and muscle MRI spectrum of AD-HNRNPDL-related myopathies demonstrating the significant particularities of the disease. We describe two new unrelated Argentinean families, carrying the previously reported c.1132G>C p.(Asp378His) HNRNPDL mutation. There was a wide phenotypic spectrum including oligo-symptomatic cases, pure limb girdle muscle involvement or distal lower limb muscle weakness. Scapular winging was the most common finding, observed in all patients. Muscle MRIs of the thigh, at different stages of the disease, showed particular involvement of adductor magnus and vastus besides a constant preservation of the rectus femoris and the adductor longus muscles, defining a novel MRI pattern. Muscle biopsy findings were characterized by the presence of numerous rimmed vacuoles, cytoplasmic bodies, and abundant autophagic material at the histochemistry and ultrastructural levels. HNRNPDL-related LGMD D3 results in a wide range of clinical phenotypes from the classic proximal form of LGMD to a more distal phenotype. Thigh MRI suggests a specific pattern. Codon 378 of HNRNPDL gene can be considered a mutation hotspot for HNRNPDL-related myopathy. Pathologically, the disease can be classified among the autophagic rimmed vacuolar myopathies as with the other multisystem proteinopathies.Peer reviewe

Growth Differentiation Factor 15 is a potential biomarker of therapeutic response for TK2 deficient myopathy

GDF-15 is a biomarker for mitochondrial diseases. We investigated the application of GDF-15 as biomarker of disease severity and response to deoxynucleoside treatment in patients with thymidine kinase 2 (TK2) deficiency and compared it to FGF-21. GDF-15 and FGF-21 were measured in serum from 24 patients with TK2 deficiency treated 1–49 months with oral deoxynucleosides. Patients were grouped according to age at treatment and biomarkers were analyzed at baseline and various time points after treatment initiation. GDF-15 was elevated on average 30-fold in children and 6-fold in adults before the start of treatment. There was a significant correlation between basal GDF-15 and severity based on pretreatment distance walked (6MWT) and weight (BMI). During treatment, GDF-15 significantly declined, and the decrease was accompanied by relevant clinical improvements. The decline was greater in the paediatric group, which included the most severe patients and showed the greatest clinical benefit, than in the adult patients. The decline of FGF-21 was less prominent and consistent. GDF-15 is a potential biomarker of severity and of therapeutic response for patients with TK2 deficiency. In addition, we show evidence of clinical benefit of deoxynucleoside treatment, especially when treatment is initiated at an early age

Growth Differentiation Factor 15 is a potential biomarker of therapeutic response for TK2 deficient myopathy

GDF-15 is a biomarker for mitochondrial diseases. We investigated the application of GDF-15 as biomarker of disease severity and response to deoxynucleoside treatment in patients with thymidine kinase 2 (TK2) deficiency and compared it to FGF-21. GDF-15 and FGF-21 were measured in serum from 24 patients with TK2 deficiency treated 1-49 months with oral deoxynucleosides. Patients were grouped according to age at treatment and biomarkers were analyzed at baseline and various time points after treatment initiation. GDF-15 was elevated on average 30-fold in children and 6-fold in adults before the start of treatment. There was a significant correlation between basal GDF-15 and severity based on pretreatment distance walked (6MWT) and weight (BMI). During treatment, GDF-15 significantly declined, and the decrease was accompanied by relevant clinical improvements. The decline was greater in the paediatric group, which included the most severe patients and showed the greatest clinical benefit, than in the adult patients. The decline of FGF-21 was less prominent and consistent. GDF-15 is a potential biomarker of severity and of therapeutic response for patients with TK2 deficiency. In addition, we show evidence of clinical benefit of deoxynucleoside treatment, especially when treatment is initiated at an early age

Histological description of the reproductive tract of male and female hatchlings of the magdalena river turtle (podocnemis lewyana).

In this study we review the macro and microscopic description of the reproductive system of male and female Podocnemis lewyana neonates of 3.5 months of age. We found macroscopic differences in the morphology of the gonads, with ovaries being twice longer that testes, and testes being twice wider than ovaries. Microscopically we identified several immature elements, such as the lack of muscle layer in the oviduct of reptile females, and simple epithelia instead of pseudostratified epithelia in the oviduct and epididymis described in adults. We also describe a black pigment observed macroscopically in the mesovarium and macroscopically and histologically in the epididymis. This pigment is compatible with the center of melano-macrophages described in other vertebrates. Finally we described a supporting mesenchymal structure, the appendage of the oviduct, with was much longer than what has been described in other Podocnemis species

Histological description of the reproductive tract of male and female hatchlings of the magdalena river turtle (podocnemis lewyana).

In this study we review the macro and microscopic description of the reproductive system of male and female Podocnemis lewyana neonates of 3.5 months of age. We found macroscopic differences in the morphology of the gonads, with ovaries being twice longer that testes, and testes being twice wider than ovaries. Microscopically we identified several immature elements, such as the lack of muscle layer in the oviduct of reptile females, and simple epithelia instead of pseudostratified epithelia in the oviduct and epididymis described in adults. We also describe a black pigment observed macroscopically in the mesovarium and macroscopically and histologically in the epididymis. This pigment is compatible with the center of melano-macrophages described in other vertebrates. Finally we described a supporting mesenchymal structure, the appendage of the oviduct, with was much longer than what has been described in other Podocnemis species

Histological description of the reproductive tract of male and female hatchlings of the magdalena river turtle (podocnemis lewyana).

In this study we review the macro and microscopic description of the reproductive system of male and female Podocnemis lewyana neonates of 3.5 months of age. We found macroscopic differences in the morphology of the gonads, with ovaries being twice longer that testes, and testes being twice wider than ovaries. Microscopically we identified several immature elements, such as the lack of muscle layer in the oviduct of reptile females, and simple epithelia instead of pseudostratified epithelia in the oviduct and epididymis described in adults. We also describe a black pigment observed macroscopically in the mesovarium and macroscopically and histologically in the epididymis. This pigment is compatible with the center of melano-macrophages described in other vertebrates. Finally we described a supporting mesenchymal structure, the appendage of the oviduct, with was much longer than what has been described in other Podocnemis species

Histological description of the reproductive tract of male and female hatchlings of the magdalena river turtle (podocnemis lewyana).

In this study we review the macro and microscopic description of the reproductive system of male and female Podocnemis lewyana neonates of 3.5 months of age. We found macroscopic differences in the morphology of the gonads, with ovaries being twice longer that testes, and testes being twice wider than ovaries. Microscopically we identified several immature elements, such as the lack of muscle layer in the oviduct of reptile females, and simple epithelia instead of pseudostratified epithelia in the oviduct and epididymis described in adults. We also describe a black pigment observed macroscopically in the mesovarium and macroscopically and histologically in the epididymis. This pigment is compatible with the center of melano-macrophages described in other vertebrates. Finally we described a supporting mesenchymal structure, the appendage of the oviduct, with was much longer than what has been described in other Podocnemis species