Quantum dot-based multiphoton fluorescent pipettes for targeted neuronal electrophysiology

Targeting visually identified neurons for electrophysiological recording is a fundamental neuroscience technique; however, its potential is hampered by poor visualization of pipette tips in deep brain tissue. We describe quantum dot-coated glass pipettes that provide strong two-photon contrast at deeper penetration depths than those achievable with current methods. We demonstrated the pipettes' utility in targeted patch-clamp recording experiments and single-cell electroporation of identified rat and mouse neurons in vitro and in vivo

Szénszállal erősített műanyag marásának vizsgálata

A szálerősítésű műanyagok használata jelentősen megnőtt az elmúlt évtizedekben. Ezzel együtt megnőtt az igény az ilyen anyagok befejező megmunkálására, azok forgácsolására. A szálerősítésű anyagok forgácsolásakor viszont számos, a fémek forgácsolási hibáitól eltérő hiba jelentkezik, ilyen a szálkiszakadás, a szálak törése a forgácsolási zónában, a mátrixanyag elkenődése és a delamináció. Jelen vizsgálat célja, hogy a szénszállal erősített műanyag marása során feltárjuk az eredő forgácsolóerő (Fe) jellemzőit a bemenő forgácsolási paraméterek függvényében. A mérések során egymásra merőleges (0–90°) szálelrendezésű anyagot martunk. A kísérlet során a középpontos kompozíciós kísérlet tervet használtuk. A kiértékelés során pedig a válaszfüggvények módszerét alkalmaztuk

Investigation of Milling of Carbon Fiber Reinforced Plastic

The use of fiber-reinforced plastics has increased significantly in the past decades. Consequently, the demand for finishing and machining of such materials has also escalated. During machining, the fiber-reinforced materials exhibit machining problems dissimilar to the problems of metals. These are fiber pull-out, fiber breakage in the cutting zone, matrix smearing and delamination. The purpose of this experiment is to in¬vestigate the characteristics of the resultant force (Fe) dur-ing the milling of carbon fiber reinforced plastic as a function of input machining parameters. For the force measurements, CFR with perpendicular (0°-90°) fiber orientation was machined. The experimental design involved the central composite design method. To analyze and evaluate the measurements, we applied the response surface methodology

Tom Jobim e a modernidade musical brasileira : 1953/1958

Orientador: Marcos NapolitanoDissertaçao (mestrado) - Universidade Federal do Paraná, Setor de Ciencias Humanas, Letras e Artes, Programa de Pós-Graduaçao em História. Defesa: Curitiba, 2004Inclui bibliografi

Robotic Automation of In Vivo Two-Photon Targeted Whole-Cell Patch-Clamp Electrophysiology

Whole-cell patch-clamp electrophysiological recording is a powerful technique for studying cellular function. While in vivo patch-clamp recording has recently benefited from automation, it is normally performed “blind,” meaning that throughput for sampling some genetically or morphologically defined cell types is unacceptably low. One solution to this problem is to use two-photon microscopy to target fluorescently labeled neurons. Combining this with robotic automation is difficult, however, as micropipette penetration induces tissue deformation, moving target cells from their initial location. Here we describe a platform for automated two-photon targeted patch-clamp recording, which solves this problem by making use of a closed loop visual servo algorithm. Our system keeps the target cell in focus while iteratively adjusting the pipette approach trajectory to compensate for tissue motion. We demonstrate platform validation with patch-clamp recordings from a variety of cells in the mouse neocortex and cerebellum