Invasive bacterial infections in Gambians with sickle cell anaemia in an era of widespread Pneumococcal and Haemophilus influenzae type B vaccination

Background: There is relatively little data on the aetiology of bacterial infections in patients with sickle cell anaemia (SCA) in West Africa, and no data from countries that have implemented conjugate vaccines against both Streptococcus pneumoniae and Haemophilus influenzae type b (Hib). Methods: We conducted a retrospective analysis of SCA patients admitted to the Medical Research Council Unit, The Gambia during a five-year period when there was high coverage of Hib and Pneumococcal conjugate vaccination. We evaluated 161 admissions of 126 patients between April 2010 and April 2015. Results: Pathogenic bacteria were identified in blood cultures from 11 of the 131 admissions that had cultures taken (8.4%, 95% CI 4.5-14.1%). The most frequent isolate was Salmonella Typhimurium (6/11; 54.5%), followed by Staphylococcus aureus (2/11; 18.2%) and other enteric Gram-negative pathogens (2/11; 18.2%) and there was one case of H. influenzae non-type b bacteraemia (1/11; 9.1%). There were no episodes of bacteraemia caused by S. pneumoniae or Hib. Conclusions: The low prevalence of S. pneumoniae and Hib, and the predominance of non-typhoidal Salmonella as a cause of bacteraemia suggest the need to reconsider optimal antimicrobial prophylaxis and the empirical treatment regimens for patients with SCA

Mechanical behaviour of sustainable concrete with waste ceramic aggregate replacement

Sustainability and material use have been becoming increasingly important in industry and academia in recent years, prompting investigations for ways to improve sustainability in construction materials. Past studies have investigated natural aggregate replacement in concrete with a variety of materials, including recycled concrete, glass, bricks, ceramics, and even automobile tires. These studies have produced varied results. Ceramic materials are a great prospective material for aggregate replacement due to their desirable mechanical properties and availability in waste streams worldwide. This study focusses on coarse aggregate replacement with ceramic tile materials of virgin and waste origins, reaching as high as 100% replacement. To limit the variability experienced in previous studies, great emphasis was placed on limiting parameters tested between series to the physical properties of the replacement ceramic materials alone. Test results thus far have shown that natural coarse aggregate can be replaced in ratios as high as 100%, with only minimal effects on the mechanical properties of the resulting concrete. Compressive, tensile, and flexural strengths have shown minor change, with only a slight increase in elastic modulus. This study shows that ceramic waste material has great potential for use in concrete when a suitable preparation method is used.published_or_final_versio

Practical Sparse Matrices in C++ with Hybrid Storage and Template-Based Expression Optimisation

Despite the importance of sparse matrices in numerous fields of science, software implementations remain difficult to use for non-expert users, generally requiring the understanding of underlying details of the chosen sparse matrix storage format. In addition, to achieve good performance, several formats may need to be used in one program, requiring explicit selection and conversion between the formats. This can be both tedious and error-prone, especially for non-expert users. Motivated by these issues, we present a user-friendly and open-source sparse matrix class for the C++ language, with a high-level application programming interface deliberately similar to the widely used MATLAB language. This facilitates prototyping directly in C++ and aids the conversion of research code into production environments. The class internally uses two main approaches to achieve efficient execution: (i) a hybrid storage framework, which automatically and seamlessly switches between three underlying storage formats (compressed sparse column, Red-Black tree, coordinate list) depending on which format is best suited and/or available for specific operations, and (ii) a template-based meta-programming framework to automatically detect and optimise execution of common expression patterns. Empirical evaluations on large sparse matrices with various densities of non-zero elements demonstrate the advantages of the hybrid storage framework and the expression optimisation mechanism.Comment: extended and revised version of an earlier conference paper arXiv:1805.0338

An atypical presentation of a common infection.

Case Report: A six-year-old girl presented with a one year history of painless, non-purulent conjunctivitis of her left eye which had been treated on two occasions with topical antibiotics with no effect

Quantitative Proteomics of Lymphocytes

Lymphocytes are the best-studied higher eukaryote cells. In this report, quantitative relationships of the protein components in resting cell, blast cell and plasma cell types are evaluated. The comparison of these cell types leads to the conclusion that resting cells synthesize about one-twentieth of the protein species as compared to blast cells. Blast cells seem to be metabolically the most robust lymphocyte type. Plasma cells are geared towards synthesis of one main product (antibody in B plasma cells), while most of the synthesis of other protein species (including those for housekeeping and repair) decreases as the messages decay. Although the data presented in this communication allow a meaningful comparison of three cell populations, they are far from providing a full picture. Both silver staining and radiofluorography depict only proteins of high or intermediate abundance. Silver staining misses most proteins present at <10 000 copies/cell, while radiofluorography misses all those proteins with slow turnover (and those with no methionine residue in their sequence). The detection of 1100 spots in the blast cell-related radiofluorograph includes visualization of some 97–99% of protein mass, but some 3900 polypeptide species in the remaining 1–3% of protein mass will pass undetected. This protein mass (0.7–2 pg) reflects some 2500–7500 copies of each of those 3900 polypeptide species that are present in the cell below the detection limit. The work emphasizes that full understanding of cellular function can be achieved only if quantitative aspects of cell inventory are considered

Mitochondria directly influence fertilisation outcome in the pig

The mitochondrion is explicitly involved in cytoplasmic regulation and is the cell's major generator of ATP. Our aim was to determine whether mitochondria alone could influence fertilisation outcome. In vitro, oocyte competence can be assessed through the presence of glucose-6-phosphate dehydrogenase (G6PD) as indicated by the dye, brilliant cresyl blue (BCB). Using porcine in vitro fertilisation (IVF), we have assessed oocyte maturation, cytoplasmic volume, fertilisation outcome, mitochondrial number as determined by mtDNA copy number, and whether mitochondria are uniformly distributed between blastomeres of each embryo. After staining with BCB, we observed a significant difference in cytoplasmic volume between BCB positive (BCB+) and BCB negative (BCB-) oocytes. There was also a significant difference in mtDNA copy number between fertilised and unfertilised oocytes and unequal mitochondrial segregation between blastomeres during early cleavage stages. Furthermore, we have supplemented BCB- oocytes with mitochondria from maternal relatives and observed a significant difference in fertilisation outcomes following both IVF and intracytoplasmic sperm injection (ICSI) between supplemented, sham-injected and non-treated BCB- oocytes. We have therefore demonstrated a relationship between oocyte maturity, cytoplasmic volume, and fertilisation outcome and mitochondrial content. These data suggest that mitochondrial number is important for fertilisation outcome and embryonic development. Furthermore, a mitochondrial pre-fertilisation threshold may ensure that, as mitochondria are diluted out during post-fertilisation cleavage, there are sufficient copies of mtDNA per blastomere to allow transmission of mtDNA to each cell of the post-implantation embryo after the initiation of mtDNA replication during the early postimplantation stages

How people with dementia use twitter: A qualitative analysis

This is the final version. Available from Elsevier via the DOI in this record. People with dementia are publicly sharing their experiences of living with the condition and acting collectively to produce social change. Social media could support them in doing this, but no previous studies have comprehensively analysed their use of Twitter. The aims of this study were to identify how people with dementia use Twitter and examine the illness identities they create and promote online. Tweetcatcher was used to collect 2774 tweets posted over six months by 12 account holders with dementia, across three countries. Tweets were analysed thematically. Six themes were identified through the analysis: nothing about us without us, collective action, experts by experience, living with dementia not suffering from it, community, and stories of dementia. On Twitter, people with dementia are developing a collective illness identity to further a social movement that is focused on improving the lives of people with dementia. They are also communicating their personal identities by documenting their lived experiences. Twitter is being used to convey positive, rather than negative, messages about dementia. The findings of this study also show that thematic analysis can be applied to micro texts that can combine over time to form longer narratives.National Institute for Health Research (NIHR) Collaboration for Leadership in Applied Health Research and Care South West Peninsula (PenCLAHRC)

Health and co-operative housing

The health of 189 women living in a tenant management co-operative based in high-rise housing in Glasgow was compared to 183 women living in a similar area under direct council management. No difference was found in the Medical Outcomes Study Instrument, a health status measure or Hospital Anxiety-Depression Rating scale. Within the co-operative,regular or occasional attendance at co-operative meetings and involvement in the co-operative was associated with better health. Attenders had better social and physical function scores and less anxiety and depression . These differences were still statistically significant after allowing for marital status, age, employment, children, loss of job due to ill health and dampness in the flat and after excluding committee members. No differences in General Practitioner consultation rates or use of hospital services were found. The hypothetical link for this attendance effect could be a rise in self-esteem in those involved in the co-operative protecting the women against illness. Women in the co-operative had a sense of political efficacy. 52 out of 189 women attended the co-operative meetings regularly or occasionally. Those living in the co-operative were more satisfied with their housing . Both areas had high levels of perceived and actual crime. Women living in flats affected by dampness ,mould or a poor state of repair (20%) had significantly lower scores on the General Health perceptions ,Mental health,Physical function and pain scales of the MOSI and more anxiety and depression on the HAD. They also consulted their general practitioners more fi-equently ,but dampness did not affect their reporting of chronic disease or hospital use. The study demonstrates that attendance at the co-operative meetings is associated with better health and less anxiety and depression. Any theoretical difference in health scores between the two housing areas may have been masked by the levels of attendance and involvement in the co-operative , the higher initial level of deprivation in the co-operative area and the relatively short time that the co-operative had been in operation. The report also contains a validation study of the Medical Outcomes Study Instrument (MOSI).It involved a postal survey of 486 women aged 30-40 years in a General Practice in the East of Glasgow using the MOSI and Nottingham Health Profile.Tests of construct validity show the MOSI to be a candidate for Health Status measurement in research and audit in primary care