Influência de reguladores de crescimento no desenvolvimento radicular de sementes de Coffea arabica L. Rubi in vitro

It was evaluated the effect of plant growth regulators in the root development in coffee seeds cv. Rubi (Coffea arabica L. Rubi) grown in vitro. Coffee seeds from adult plants were established on medium MS/2 + sucrose (20 g L-1) + agar (6 g L-1). Four treatments were tested: T1 - control; T2 -BAP (6 mg L-1) + NAA (0,1 mg L-1); T3 -BAP (6 mg L-1); T4 - GA3 (5 mg L-1). The highest rooting response was observed on the control (MS/2 + agar + sucrose). This suggest that rooting can be achieved without the use of plant growth regulators.Avaliou-se o efeito de reguladores de crescimento na germinação e enraizamento in vitro de sementes de cafeeiro Rubi (Coffea arabica L. Rubi ). Sementes de plantas adultas de cafeeiro foram inoculadas em meio MS/2 + 20 g L-1 de sacarose e 6 g L-1 de ágar. Foram testados quatro tratamentos: T1 - controle; T2 - 6 mg L-1 de BAP + 0,1 mg L-1 de ANA; T3- 6 mg L-1 de BAP; T4 - 5 mg L-1 de GA3. O melhor tratamento obtido para enraizamento de sementes de cafeeiro foi o controle (MS/2 + ágar + sacarose), tornando-se desnecessária a adição de reguladores de crescimento

Comparison of the Effects of Maropitant, Lidocaine, and Dextroketamine Administered by the Intratesticular Route in Dogs Undergoing Elective Orchiectomy

Background: Balanced anesthesia achieved with combinations of inhaled and injectable drugs administered systemically or in loco-regional anesthetic blocks, is widely used in veterinary medicine. The use of anesthesia and/or local analgesia has already demonstrated benefits in the performance of elective orchiectomy in different species, there is no literature that evaluates the use of the maropitant intratesticular route. The present study evaluated the cardiorespiratory variables and analgesia produced by intratesticular blockade with maropitant, lidocaine, or dextroketamine during the trans-operative period along with the discharge and anesthetic recovery of dogs that underwent elective orchiectomy. Materials, Methods & Results: Used twenty-four dogs from routine elective orchiectomy, considered healthy based on the results of clinical and hematological tests. The animals were randomly divided into three groups and was applied intratesticularly 2% lidocaine at a dose of 1 mg/kg (GL), 5% dextrocetamina at a dose of 2.5 mg/kg (GC), or 1% maropitant  at a dose of 1 mg/kg (GM). Anesthesia induction was performed with propofol (to effect), and stabilization of inhalational anesthesia was achieved with 1.7 V% of sevoflurane diluted in 100% oxygen administered through a calibrated vaporizer and appropriate anesthetic system based on the animal's weight, being kept under spontaneous ventilation, After induction, we waited 10 min for stabilization of exhaled anesthetic concentration and then administered one of the treatments intratesticularly. After five min from the local block the surgical procedure was started during up to 15 min. Heart rate (HR), respiratory rate (RR), systolic arterial pressure (SAP), diastolic arterial pressure (DAP), mean arterial pressure (MAP), oxygen saturation of hemoglobin (SatO2), end-tidal carbon dioxide partial pressure (EtCO2), end-tidal sevoflurane concentration (EtSevo) in exhaled air, and body temperature (T°C) were measured before intratesticular administration of the agent and at specific time points during surgery, in addition to assessments of analgesia and evaluation of discharge and anesthetic recovery. Statistical analysis was performed using the Kolmogorov-Smirnov test (P > 0.10). The parameters HR, RR, SAP, DAP, MAP, T, EtCO2, and SatO2 were evaluated by analysis of variance (ANOVA), followed by the Scott-Knott test (Software R® 3.2.0 - 2013), considering a significance level of P < 0.05. The results did not show inter-drug differences for the evaluated variables.Discussion: It was decided not to use premedication (MPA), so that we could accurately assess the analgesic effect of drugs, lidocaine, dextroketamine and maropitant, on intratesticular block during elective orchiectomy. In this study we demonstrated that these drugs promoted analgesia, because the trans-surgical values were relatively lower compared to baseline and within physiological limits for the species. In addition, it was noted that local analgesia used was efficient since even at the time of ligature and the incision spermatic cord which is described in the literature as the most painful part of the surgical procedure. The post-anesthetic recovery and discharge were quick in the absence of MPA, local analgesia without residual effects and the use of sevoflurane. The sevoflurane has a very low blood gas coefficient solubility, resulting in a recovery fast. Therefore, we can conclude that all of the drugs promoted analgesia and cardiorespiratory stability as well as rapid anesthetic recovery for elective orchiectomy in this species

The management and effectiveness of professionalization: An educational-based Epistemical study

This academic paper was developed with the aim of analyzing the management challenges for the effectiveness of professionalization. Managers of companies and educational units must contain all their demands in a unit in the administrative sector, so that there are no gaps or divisions in the separation of problems, such as lack of communication and difficulty in working as a team. Therefore, thinking about the actions of managers is immensely important for a good forwarding of professionalization. This is a bibliographical and documentary research, with a qualitative interpretive nature. With regard to the conditions for the effectiveness of the management process in the Brazilian business and educational fields, it became effective for us to carry out a bibliographic survey of existing studies on the subject since the turn of the 21st century. It is argued, therefore, that it is necessary to adopt a more professional and qualified management for the development and concreteness of the professional subject, as well as all those involved in the administrative system

Quantitative analysis of bone marrow in pancytopenic dogs

The pancytopenia can be associated with intra and extra medullary disorders. When the etiology is not obvious, the examination of bone marrow is necessary. The study aims to report and discuss quantitative amendments in bone marrow and their causes in dogs with pancytopenia. Bone marrow aspirate was obtained from 65 dogs with pancytopenia over a period of 13 consecutive months for preparation of smears stained by Giemsa and observed in light microscopy. Five hundred cells differential count was held; and assessed myeloid:erythroid ratio, cellularity, megakaryocytes and direct parasitological examination. The data were evaluated by the Chi-square statistical test. Blood tests of 3120 canines with several clinical changes were analyzed to identify pancytopenia, which accounted for 167 (5.4%) dogs. Interpretation of quantitative characteristics was carried out from the bone marrow smear in 65 pancytopenic dogs and the etiology was established in 40 (61.5%) of these, which included infection by E. canis and L. chagasi, idiopathic aplastic anemia, chronic renal failure and co-infections. In 17 (26.2%) animals were not observed medullary changes. The most bone marrow change was observed myeloid and erythroid hypoplasia in 17 (26.2%) dogs, followed by myeloid and erythroid hyperplasia (24.6%). The myelogram association to the blood cell count allowed the identification of medullary findings and its participation on the occurrence of pancytopenia cases. The number of cases resulting from infectious diseases was 38 (58.5%) in canine assessed: significant number due to its endemic characteristic of the study region

DISTRIBUIÇÃO ESPACIAL DA BROCA-DO-ESTIPE-DO-COQUEIRO

O objetivo desta pesquisa foi estudar a distribuição espacial de Rhinostomus barbirostris (Coleoptera: Curculionidae) (Fabricius, 1775) em coqueiro (Cocos nucifera L.). Foram realizadas 10 amostragens quinzenalmente do número de machos, fêmeas e total (macho + fêmea), de R. barbirostris em armadilhas demarcada com uma tela de náilon envoltas as palmeiras com sintoma característico do ataque dessa praga, totalizando 24 armadilhas. Para o estudo da distribuição espacial de R. barbirostris, foram calculados os índices de dispersão: razão variância/média (I), índice de Morisita (), coeficiente de Green (Cx), expoente k da distribuição binomial negativa (k), índice de grupo por tamanho (IGT), Índice de frequência de agrupamento (IFA) e Índice de Patchiness (IP) para cada amostragem. Foi testado o ajuste dos dados as distribuições Poisson e binomial negativa. Os resultados obtidos nos índices de agregação calculados indicam que a maioria das amostragens apresentou distribuição agregada de R. barbirostris, para todas as variáveis estudadas. A distribuição binomial negativa foi o modelo mais adequado para representar a distribuição de frequência da coleobroca no coqueiro, já que a variância foi superior à média na maioria das amostragens.Palavras-chave: Rhinostomus barbirostris; cocoicultura; índices de dispersão. SPATIAL DISTRIBUTION OF COCONUT BORER ABSTRACT: The objective of this research was to study the spatial distribution of Rhinostomus barbirostris (Coleoptera: Curculionidae) (Fabricius, 1775) in coconut (Cocos nucifera L.). They were made ten samples every two weeks,of males,  females and the total (Male + female), R. barbirostris in marked traps with a nylon screen surrounded with palm trees characteristic symptom of the attack of this pest, totaling 24 traps. To study the spatial distribution of R. barbirostris, dispersion indexes were calculated: variance / mean ratio, Morisita index , Green coefficient (Cx), k exponent of negative binomial distribution, index of de Cluster Size (ICS), index of de Cluster Frequency (ICF) e index of de Patchiness (IP) for each sample. It tested the data fitting the Poisson distribution and binomial negative. The results calculated in aggregation indexes indicate that Most samplings presented aggregated distribution of R. barbirostris for all the variables studied. The negative binomial distribution was the best model to represent the frequency distribution of the coconut borer, since the variance was higher than the average for the majority of the samples.Keywords: Rhinostomus barbirostris; coconut farmin; dispersion indexes

Panoramic snapshot of serum soluble mediator interplay in pregnant women with convalescent COVID-19: an exploratory study

IntroductionSARS-CoV-2 infection during pregnancy can induce changes in the maternal immune response, with effects on pregnancy outcome and offspring. This is a cross-sectional observational study designed to characterize the immunological status of pregnant women with convalescent COVID-19 at distinct pregnancy trimesters. The study focused on providing a clear snapshot of the interplay among serum soluble mediators.MethodsA sample of 141 pregnant women from all prenatal periods (1st, 2nd and 3rd trimesters) comprised patients with convalescent SARS-CoV-2 infection at 3-20 weeks after symptoms onset (COVID, n=89) and a control group of pre-pandemic non-infected pregnant women (HC, n=52). Chemokine, pro-inflammatory/regulatory cytokine and growth factor levels were quantified by a high-throughput microbeads array.ResultsIn the HC group, most serum soluble mediators progressively decreased towards the 2nd and 3rd trimesters of pregnancy, while higher chemokine, cytokine and growth factor levels were observed in the COVID patient group. Serum soluble mediator signatures and heatmap analysis pointed out that the major increase observed in the COVID group related to pro-inflammatory cytokines (IL-6, TNF-α, IL-12, IFN-γ and IL-17). A larger set of biomarkers displayed an increased COVID/HC ratio towards the 2nd (3x increase) and the 3rd (3x to 15x increase) trimesters. Integrative network analysis demonstrated that HC pregnancy evolves with decreasing connectivity between pairs of serum soluble mediators towards the 3rd trimester. Although the COVID group exhibited a similar profile, the number of connections was remarkably lower throughout the pregnancy. Meanwhile, IL-1Ra, IL-10 and GM-CSF presented a preserved number of correlations (≥5 strong correlations in HC and COVID), IL-17, FGF-basic and VEGF lost connectivity throughout the pregnancy. IL-6 and CXCL8 were included in a set of acquired attributes, named COVID-selective (≥5 strong correlations in COVID and <5 in HC) observed at the 3rd pregnancy trimester.Discussion and conclusionFrom an overall perspective, a pronounced increase in serum levels of soluble mediators with decreased network interplay between them demonstrated an imbalanced immune response in convalescent COVID-19 infection during pregnancy that may contribute to the management of, or indeed recovery from, late complications in the post-symptomatic phase of the SARS-CoV-2 infection in pregnant women

Pervasive gaps in Amazonian ecological research

Biodiversity loss is one of the main challenges of our time,1,2 and attempts to address it require a clear un derstanding of how ecological communities respond to environmental change across time and space.3,4 While the increasing availability of global databases on ecological communities has advanced our knowledge of biodiversity sensitivity to environmental changes,5–7 vast areas of the tropics remain understudied.8–11 In the American tropics, Amazonia stands out as the world’s most diverse rainforest and the primary source of Neotropical biodiversity,12 but it remains among the least known forests in America and is often underrepre sented in biodiversity databases.13–15 To worsen this situation, human-induced modifications16,17 may elim inate pieces of the Amazon’s biodiversity puzzle before we can use them to understand how ecological com munities are responding. To increase generalization and applicability of biodiversity knowledge,18,19 it is thus crucial to reduce biases in ecological research, particularly in regions projected to face the most pronounced environmental changes. We integrate ecological community metadata of 7,694 sampling sites for multiple or ganism groups in a machine learning model framework to map the research probability across the Brazilian Amazonia, while identifying the region’s vulnerability to environmental change. 15%–18% of the most ne glected areas in ecological research are expected to experience severe climate or land use changes by 2050. This means that unless we take immediate action, we will not be able to establish their current status, much less monitor how it is changing and what is being lostinfo:eu-repo/semantics/publishedVersio