In-Space Propulsion Technology Products Ready for Infusion on NASA's Future Science Missions

Since 2001, the In-Space Propulsion Technology (ISPT) program has been developing and delivering in-space propulsion technologies that will enable or enhance NASA robotic science missions. These in-space propulsion technologies are applicable, and potentially enabling, for future NASA flagship and sample return missions currently being considered. They have a broad applicability to future competed mission solicitations. The high-temperature Advanced Material Bipropellant Rocket (AMBR) engine, providing higher performance for lower cost, was completed in 2009. Two other ISPT technologies are nearing completion of their technology development phase: 1) NASA s Evolutionary Xenon Thruster (NEXT) ion propulsion system, a 0.6-7 kW throttle-able gridded ion system; and 2) Aerocapture technology development with investments in a family of thermal protection system (TPS) materials and structures; guidance, navigation, and control (GN&C) models of blunt-body rigid aeroshells; aerothermal effect models; and atmospheric models for Earth, Titan, Mars and Venus. This paper provides status of the technology development, applicability, and availability of in-space propulsion technologies that have recently completed their technology development and will be ready for infusion into NASA s Discovery, New Frontiers, SMD Flagship, or technology demonstration missions

Constitutive Expression of PU.1 in Fetal Hematopoietic Progenitors Blocks T Cell Development at the Pro-T Cell Stage

AbstractThe essential hematopoietic transcription factor PU.1 is expressed in multipotent thymic precursors but downregulated during T lineage commitment. The significance of PU.1 downregulation was tested using retroviral vectors to force hematopoietic precursors to maintain PU.1 expression during differentiation in fetal thymic organ culture. PU.1 reduced thymocyte expansion and blocked development at the pro-T cell stage. PU.1-expressing cells could be rescued by switching to conditions permissive for macrophage development; thus, the inhibition depends on both lineage and developmental stage. An intact DNA binding domain was required for these effects. PU.1 expression can downregulate pre-Tα, Rag-1, and Rag-2 in a dose-dependent manner, and higher PU.1 levels induce Mac-1 and Id-2. Thus, downregulation of PU.1 is specifically required for progression in the T cell lineage

Quantification of Seasonal Long Physical Load in Soccer Players With Different Starting Status From the English Premier League: Implications for Maintaining Squad Physical Fitness.

PURPOSE: To quantify the accumulative training and match load during an annual season in English Premier League soccer players classified as starters (n=8, started ≥60% of games), fringe players (n=7, started 30-60% of games) and non-starters (n=4, started 25.2 km/h (11.2 ± 4.2, v 2.9 ± 1.2 km; ES=2.3) than non-starters. Additionally, starters also completed more sprinting (P<0.01. ES=2.0) than fringe players who accumulated 4.5 ± 1.8 km. Such differences in total high-intensity physical work done were reflective of differences in actual game time between playing groups as opposed to differences in high-intensity loading patterns during training sessions. CONCLUSIONS: Unlike total seasonal volume of training (i.e. total distance and duration), seasonal high-intensity loading patterns are dependent on players' match starting status thereby having potential implications for training programme design

TIM3+FOXP3+ regulatory T cells are tissue-specific promoters of T-cell dysfunction in cancer

T-cell immunoglobulin mucin 3 (TIM3) is an inhibitory molecule that has emerged as a key regulator of dysfunctional or exhausted CD8+ T cells arising in chronic diseases such as cancer. In addition to exhausted CD8+ T cells, highly suppressive regulatory T cells (Tregs) represent a significant barrier against the induction of antitumor immunity. We have found that the majority of intratumoral FOXP3+ Tregs express TIM3. TIM3+ Tregs co-express PD-1, are highly suppressive and comprise a specialized subset of tissue Tregs that are rarely observed in the peripheral tissues or blood of tumor-bearing mice. The co-blockade of the TIM3 and PD-1 signaling pathways in vivo results in the downregulation of molecules associated with TIM3+ Treg suppressor functions. This suggests that the potent clinical efficacy of co-blocking TIM3 and PD-1 signal transduction cascades likely stems from the reversal of T-cell exhaustion combined with the inhibition of regulatory T-cell function in tumor tissues. Interestingly, we find that TIM3+ Tregs accumulate in the tumor tissue prior to the appearance of exhausted CD8+ T cells, and that the depletion of Tregs at this stage interferes with the development of the exhausted phenotype by CD8+ T cells. Collectively, our data indicate that TIM3 marks highly suppressive tissue-resident Tregs that play an important role in shaping the antitumor immune response in situ, increasing the value of TIM3-targeting therapeutic strategies against cancer

Subversion of T lineage commitment by PU.1 in a clonal cell line system

Specification of mammalian T lymphocytes involves prolonged developmental plasticity even after lineage-specific gene expression begins. Expression of transcription factor PU.1 may maintain some myeloid-like developmental alternatives until commitment. Commitment could reflect PU.1 shutoff, resistance to PU.1 effects, and/or imposition of a suicide penalty for diversion. Here, we describe subclones from the SCID.adh murine thymic lymphoma, adh.2C2 and adh.6D4, that represent a new tool for probing these mechanisms. PU.1 can induce many adh.2C2 cells to undergo diversion to a myeloid-like phenotype, in an all-or-none fashion with multiple, coordinate gene expression changes; adh.6D4 cells resist diversion, and most die. Diversion depends on the PU.1 Ets domain but not on known interactions in the PEST or Q-rich domains, although the Q-rich domain enhances diversion frequency. Protein kinase C/MAP kinase stimulation can make adh.6D4 cells permissive for diversion without protecting from suicide. These results show distinct roles for regulated cell death and another stimulation-sensitive function that establishes a threshold for diversion competence. PU.1 also diverts normal T-cell precursors from wild type or Bcl2-transgenic mice to a myeloid-like phenotype, upon transduction in short-term culture. The adh.2C2 and adh.6D4 clones thus provide an accessible system for defining mechanisms controlling developmental plasticity in early T-cell development

Action-derived molecular dynamics in the study of rare events

We present a practical method to generate classical trajectories with fixed initial and final boundary conditions. Our method is based on the minimization of a suitably defined discretized action. The method finds its most natural application in the study of rare events. Its capabilities are illustrated by non-trivial examples. The algorithm lends itself to straightforward parallelization, and when combined with molecular dynamics (MD) it promises to offer a powerful tool for the study of chemical reactions.Comment: 7 Pages, 4 Figures (3 in color), submitted to Phys. Rev. Let

Some triviality results for quasi-Einstein manifolds and Einstein warped products

In this paper we prove a number of triviality results for Einstein warped products and quasi-Einstein manifolds using different techniques and under assumptions of various nature. In particular we obtain and exploit gradient estimates for solutions of weighted Poisson-type equations and adaptations to the weighted setting of some Liouville-type theorems.Comment: 15 pages, fixed minor mistakes in Section

STAR FORMATION HISTORY OF THE SMALL MAGELLANIC CLOUD: SIX HUBBLE SPACE TELESCOPE/ADVANCED CAMERA FOR SURVEY FIELDS

We observed six fields of the Small Magellanic Cloud (SMC) with the Advanced Camera for Survey on board the Hubble Space Telescope in the F555W and F814W filters. These fields sample regions characterized by very different star and gas densities, and, possibly, by different evolutionary histories. We find that the SMC was already forming stars similar to 12 Gyr ago, even if the lack of a clear horizontal branch suggests that in the first few billion years the star formation activity was low. Within the uncertainties of our two-band photometry, we find evidence of a radial variation in chemical enrichment, with the SMC outskirts characterized by lower metallicity than the central zones. From our color-magnitude diagrams, we also infer that the SMC formed stars over a long interval of time until similar to 2-3 Gyr ago. After a period of modest activity, star formation increased again in the recent past, especially in the bar and the wing of the SMC, where we see an enhancement in the star-formation activity starting from similar to 500 Myr ago. The inhomogeneous distribution of stars younger than similar to 100 Myr indicates that recent star formation has mainly developed locally

Tropospheric Airborne Meteorological Data and Reporting (TAMDAR) Icing Sensor Performance during the 2003/2004 Alliance Icing Research Study (AIRS II)

NASA Langley Research Center and its research partners from the University of North Dakota (UND) and the National Center for Atmospheric Research (NCAR) participated in the AIRS II campaign from November 17 to December 17, 2003. AIRS II provided the opportunity to compare TAMDAR in situ in-flight icing condition assessments with in situ data from the UND Citation II aircraft's Rosemont system. TAMDAR is designed to provide a general warning of ice accretion and to report it directly into the Meteorological Data Communications and Reporting System (MDCRS). In addition to evaluating TAMDAR with microphysical data obtained by the Citation II, this study also compares these data to the NWS operational in-flight icing Current Icing Potential (CIP) graphic product and with the NASA Advanced Satellite Aviation-weather Products (ASAP) Icing Severity product. The CIP and ASAP graphics are also examined in this study to provide a context for the Citation II's sorties in AIRS II

A Three-Stage Search for Supermassive Black Hole Binaries in LISA Data

Gravitational waves from the inspiral and coalescence of supermassive black-hole (SMBH) binaries with masses ~10^6 Msun are likely to be among the strongest sources for the Laser Interferometer Space Antenna (LISA). We describe a three-stage data-analysis pipeline designed to search for and measure the parameters of SMBH binaries in LISA data. The first stage uses a time-frequency track-search method to search for inspiral signals and provide a coarse estimate of the black-hole masses m_1, m_2 and of the coalescence time of the binary t_c. The second stage uses a sequence of matched-filter template banks, seeded by the first stage, to improve the measurement accuracy of the masses and coalescence time. Finally, a Markov Chain Monte Carlo search is used to estimate all nine physical parameters of the binary. Using results from the second stage substantially shortens the Markov Chain burn-in time and allows us to determine the number of SMBH-binary signals in the data before starting parameter estimation. We demonstrate our analysis pipeline using simulated data from the first LISA Mock Data Challenge. We discuss our plan for improving this pipeline and the challenges that will be faced in real LISA data analysis.Comment: 12 pages, 3 figures, submitted to Proceedings of GWDAW-11 (Berlin, Dec. '06