Detecting metal-rich intermediate-age globular clusters in NGC4570 using K-band photometry

“The original publication is available at www.springerlink.com”. Copyright Springer. DOI: 10.1007/s10509-009-0093-8Globular cluster systems (GCSs) of most early-type galaxies feature two peaks in their optical colour distributions. Blue-peak globular clusters (GCs) are believed to be old and metal-poor, whereas the ages, metallicities, and the origin of the red-peak GCs are still being debated. We obtained deep K-band photometry and combined it with Hubble Space Telescope observations in g and z to yield a full spectral energy distribution from the optical to the near-infrared. This now allows us to break the age–metallicity degeneracy. We used our evolutionary synthesis models galev for star clusters to compute a large grid of models with different metallicities and a wide range of ages. Comparing these models to our observations revealed a large population of intermediate-age (1–3 Gyr) and metal-rich (≈solar-metallicity) GCs, that will give us further insights into the formation history of this galaxy.Peer reviewe

Colour-colour diagrams and extragalactic globular cluster ages. Systematic uncertainties using the (V-K)-(V-I) diagram

(abridged) We investigate biases in cluster ages and [Fe/H] estimated from the (V-K)-(V-I) diagram, arising from inconsistent Horizontal Branch morphology, metal mixture, treatment of core convection between observed clusters and the theoretical colour grid employed for age and metallicity determinations. We also study the role played by statistical fluctuations of the observed colours, caused by the low total mass of typical globulars. Horizontal Branch morphology is potentially the largest source of uncertainty. A single-age system harbouring a large fraction of clusters with an HB morphology systematically bluer than the one accounted for in the theoretical colour grid, can simulate a bimodal population with an age difference as large as 8 Gyr. When only the redder clusters are considered, this uncertainty is almost negligible, unless there is an extreme mass loss along the Red Giant Branch phase. The metal mixture affects mainly the redder clusters; the effect of colour fluctuations becomes negligible for the redder clusters, or when the integrated Mv is brighter than -8.5 mag. The treatment of core convection is relevant for ages below ~4 Gyr. The retrieved [Fe/H] distributions are overall only mildly affected. Colour fluctuations and convective core extension have the largest effect. When 1sigma photometric errors reach 0.10 mag, all biases found in our analysis are erased, and bimodal age populations with age differences of up to ~8 Gyr go undetected. The use of both (U-I)-(V-K) and (V-I)-(V-K) diagrams may help disclosing the presence of blue HB stars unaccounted for in the theoretical colour calibration.Comment: 20 pages, including 26 figures. A&A in pres

Binding of Plasmodium falciparum to CD36 can be shielded by the glycocalyx

Abstract Background Plasmodium falciparum-infected erythrocytes sequester in the microcirculation due to interaction between surface-expressed parasite proteins and endothelial receptors. Endothelial cells are covered in a carbohydrate-rich glycocalyx that shields against undesired leukocyte adhesion. It was investigated if the cellular glycocalyx affects the binding of P. falciparum-infected erythrocytes to CD36 in vitro. Methods Glycocalyx growth was followed in vitro by using azido sugars and cationized ferritin detecting O-glycoproteins and negatively charged proteoglycans, respectively. P. falciparum (clone FCR3/IT) was selected on Chinese hamster ovary (CHO) cells transfected with human CD36. Cytoadhesion to CHO CD36 at 1–4 days after seeding was quantified by using a static binding assay. Results The glycocalyx thickness of CHO cells increased during 4 days in culture as assessed by metabolic labelling of glycans with azido sugars and with electron microscopy studying the binding of cationized ferritin to cell surfaces. The functional importance of this process was addressed in binding assays by using CHO cells transfected with CD36. In parallel with the maturation of the glycocalyx, antibody-binding to CD36 was inhibited, despite stable expression of CD36. P. falciparum selected for CD36-binding recognized CD36 on CHO cells on the first day in culture, but the binding was lost after 2–4 days. Conclusion The endothelial glycocalyx affects parasite cytoadhesion in vitro, an effect that has previously been ignored. The previously reported loss of glycocalyx during experimental malaria may play an important role in the pathogenesis of malaria complications by allowing the close interaction between infected erythrocytes and endothelial receptors

Glucagon-like peptide-1 analogue, liraglutide, in experimental cerebral malaria: implications for the role of oxidative stress in cerebral malaria

BACKGROUND: Cerebral malaria from Plasmodium falciparum infection is major cause of death in the tropics. The pathogenesis of the disease is complex and the contribution of reactive oxygen and nitrogen species (ROS/RNS) in the brain is incompletely understood. Insulinotropic glucagon-like peptide-1 (GLP-1) mimetics have potent neuroprotective effects in animal models of neuropathology associated with ROS/RNS dysfunction. This study investigates the effect of the GLP-1 analogue, liraglutide against the clinical outcome of experimental cerebral malaria (ECM) and Plasmodium falciparum growth. Furthermore the role of oxidative stress on ECM pathogenesis is evaluated. METHODS: ECM was induced in Plasmodium berghei ANKA-infected C57Bl/6j mice. Infected Balb/c (non-cerebral malaria) and uninfected C57Bl/6j mice were included as controls. Mice were treated twice-daily with vehicle or liraglutide (200 μg/kg). ROS/RNS were quantified with in vivo imaging and further analyzed ex vivo. Brains were assayed for cAMP, activation of cAMP response element binding protein (CREB) and nitrate/nitrite. Plasmodium falciparum was cultivated in vitro with increasing doses of liraglutide and growth and metabolism were quantified. RESULTS: The development and progression of ECM was not affected by liraglutide. Indeed, although ROS/RNS were increased in peripheral organs, ROS/RNS generation was not present in the brain. Interestingly, CREB was activated in the ECM brain and may protect against ROS/RNS stress. Parasite growth was not adversely affected by liraglutide in mice or in P. falciparum cultures indicating safety should not be a concern in type-II diabetics in endemic regions. CONCLUSIONS: Despite the breadth of models where GLP-1 is neuroprotective, ECM was not affected by liraglutide providing important insight into the pathogenesis of ECM. Furthermore, ECM does not induce excess ROS/RNS in the brain potentially associated with activation of the CREB system

Systemic and cerebral vascular endothelial growth factor levels increase in murine cerebral malaria along with increased Calpain and caspase activity and can be reduced by erythropoietin treatment

The pathogenesis of cerebral malaria (CM) includes compromised microvascular perfusion, increased inflammation, cytoadhesion, and endothelial activation. These events cause blood–brain barrier disruption and neuropathology and associations with the vascular endothelial growth factor (VEGF) signaling pathway have been shown. We studied this pathway in mice infected with Plasmodium berghei ANKA causing murine CM with or without the use of erythropoietin (EPO) as adjunct therapy. ELISA and western blotting was used for quantification of VEGF and relevant proteins in brain and plasma. CM increased levels of VEGF in brain and plasma and decreased plasma levels of soluble VEGF receptor 2. EPO treatment normalized VEGF receptor 2 levels and reduced brain VEGF levels. Hypoxia-inducible factor (HIF)-1α was significantly upregulated whereas cerebral HIF-2α and EPO levels remained unchanged. Furthermore, we noticed increased caspase-3 and calpain activity in terminally ill mice, as measured by protease-specific cleavage of α-spectrin and p35. In conclusion, we detected increased cerebral and systemic VEGF as well as HIF-1α, which in the brain were reduced to normal in EPO-treated mice. Also caspase and calpain activity was reduced markedly in EPO-treated mice

New insights into the star formation histories of candidate intermediate-age early-type galaxies from K'-band imaging of globular clusters

We investigate age and metallicity distributions of bright globular clusters (GCs) in the candidate intermediate-age early-type galaxies NGC 3610, NGC 584 and NGC 3377 using a combination of new Gemini/NIRI K'-band imaging and existing optical V,I photometry from HST data. The V-I vs I-K' colour-colour diagram is found to break the age-metallicity degeneracy present in optical colours, as I-K' primarily measures a populations' metallicity and is relatively insensitive, unlike optical spectroscopy, to the effect of hot horizontal branch (HB) stars, known to be present in massive old GCs. We derive GCs' photometric age, Z and masses. In general, metal-poor ([Z/H]<-0.7dex) GCs are older than more metal-rich GCs. For the most massive GCs (M>6x10^5 M_sol) in NGC 3610 with available spectroscopic data, photometric ages are older by ~2 Gyr, and this difference is more pronounced for the metal-poor GCs. However, photometric and spectroscopic metallicities are in good agreement. We suggest that this indicates the presence of a hot HB in these massive clusters, which renders spectroscopic ages from Balmer line strengths to be underestimated. To support this suggestion we show that all Galactic GCs with M>6x10^5 M_sol feature hot HBs, except 47 Tuc. Using the relation between the most massive GC mass and the galaxy's SFR, we find that the galaxies' peak SFR was attained at the epoch of the formation of the oldest (metal-poor) GCs. Age and [Z/H] distributions of the metal-rich GCs are broad, indicating prolonged galaxy star formation histories. The peak value of the age and [Z/H] distributions of the GCs correlates with host galaxy integrated age and [Z/H], showing that GCs can indeed be used as relevant proxies of the star formation histories of galaxies.(Abridged)Comment: Accepted by MNRAS; 18 pages, 12 figures, 6 tables (v2 added references

Attitudes and Performance: An Analysis of Russian Workers

This paper investigates the relationship between locus of control and performance among Russian employees, using survey data collected at 28 workplaces in 2002 in Taganrog and at 47 workplaces in 2003 in Ekaterinburg. We develop a measure that allows us to categorize the Russian employees participating in our survey as exhibiting an internal or external locus of control. We then assess the extent to which there are significant differences between “internals” and “externals” in work-related attitudes that may affect performance. In particular, we focus on (1) attitudes about outcomes associated with hard work, (2) level of job satisfaction, (3) expectation of receiving a desired reward, and (4) loyalty to and involvement with one’s organization. In each case we identify where gender and generational differences emerge. Our main objective is to determine whether Russian employees who exhibit an internal locus of control perform better than employees with an external locus of control. Our performance measures include earnings, expected promotions, and assessments of the quantity and quality of work in comparison to others at the same organization doing a similar job. Controlling for a variety of worker characteristics, we find that (1) individuals who exhibit an internal locus of control perform better, but this result is not always statistically significant; (2) even among “internals,” women earn significantly less than men and have a much lower expectation of promotion; (3) even among “internals,” experience with unemployment has a negative influence on performance.http://deepblue.lib.umich.edu/bitstream/2027.42/40144/3/wp758.pd

Do dusty A stars exhibit accretion signatures in their photospheres?

We determined abundances of O, Ca, Fe, Ba and Y for a sample of dusty and dust-free A stars, taken from the list of Cheng et al. (1992). Five of the stars have an infrared-excess due to circumstellar dust. Ongoing accretion from their circumstellar surroundings might have modified the abundances in the photospheres of these stars, but our results clearly show, that there is no difference in the photospheric composition of the dusty and dust-free stars. Instead all of them show the typical diffusion pattern which diminishes towards larger rotational velocities.Comment: 8 pages, 3 figures, accepted for publication in A&