Asbestose pulmonar: mecanismos celulares da sua patogenia

A asbestose pulmonar continua a ser uma das doenças ocupacionais mais graves do nosso tempo. Apesar de clínica e epidemiologicamente bem caracterizada pouco se sabe ainda dos mecanismos celulares responsáveis pela sua ocorrência. Assim, a partir da informação até ao momento disponível sobre este assunto, tentámos delinear os vários mecanismos envolvidos na resposta do pulmão profundo à inalação de fibras de asbesto. Numa primeira parte caracterizam-se os vários tipos de fibras de asbesto existentes bem como os seus diferentes padrões de deposição e transporte. Numa segunda parte descrevem-se os possíveis mecanismos celulares envolvidos na patogenia da asbestose pulmonar, nomeadamente a acção dos macrófagos alveolares, dos neutrófilos e dos respectivos mediadores. A análise do perfil linfocitário de lavados bronco-alveolares de pacientes com asbestose é também descrita, salientando-se um acentuado aumento na relação CD4/CD8.Pulmonary asbestosis is still one serious occupational disease ofour times. Although the clinical and epidemiological featu res ofthe disease are well caracterized, the cellular mechanisms involved are still poorly understood. Taking advantage ofall the information available on this subject, we tried to depict the several mechanisms involved in the answer ofthe deep lung to the inhalation ofasbestos jibers. ln the jirst part ofthis article we characterized the diferent kinds ofasbestos jibers as well as their patterns oftransport and deposition in the deep lung.ln the second part we discribed the possible cellular mechanisms underlying the pathogeny of pulmonary asbestosis. The e.fJect ofthe alveolar macrophages, polinuclear neuthrophils and the action oftheir mediators is discribed. Analysis of the bronco alveolar lavage jluid lynphocitary profile of pacients with pulmonary asbestosis is also discribed, revealing significant increase in the CD4/CD8 ratio

Uma prática de ensino remoto na escola pública municipal Autran Nunes

This paper aims to present the remote teaching experience of the teacher Ana Beatriz Bezerra Gomes, who mediated her classes over the Internet through WhatsApp and Google Meet in the 5th grade class of the Autran Nunes Municipal School in Fortaleza, Ceará, Brazil, in times of pandemic. The educational practice was quite atypical, as it did not fit into a classical teaching methodology, since it was neither traditional nor a methodology organized in the parameters required by Information and Communication Digital Technologies. In the analysis, we were inspired by the concepts and theories discussed by the Common National Curricular Base; the Digital Information and Communication Technologies; and remote teaching. As a result, we have a brief analysis of what happened with most teachers in this network, with the caveats of each school context, each educational practice and each teacher.Este trabalho objetiva apresentar a experiência de ensino remoto da professora Ana Beatriz Bezerra Gomes que mediou as suas aulas pela internet por meio do WhatsApp e pelo Google Meet na turma do 5o ano da Escola Municipal Autran Nunes em Fortaleza, Ceará, Brasil, em tempos de pandemia. A prática educativa foi bastante atípica, por não se enquadrar em uma metodologia clássica de ensino, já que não se tratava de metodologia tradicional e nem de uma metodologia organizada nos parâmetros exigidos pelas Tecnologias Digitais da Informação e Comunicação. Na análise, nos inspiramos nos conceitos e teorias discutidas pela Base Nacional Comum Curricular; nas Tecnologias Digitais da Informação e Comunicação e no ensino remoto. Como resultado, temos uma breve análise do que ocorreu com a maioria dos professores dessa rede, com as ressalvas de cada contexto escolar, de cada prática educativa e de cada professor(a)

Linhas de trabalho para a Educação Pré-Escolar

UID/LIN/03213/2013 PEst-OE/LIN/UI3213/2014Este documento foi elaborado no âmbito do “Estudo de avaliação do impacto da aplicação do Português Língua Não Materna no sistema educativo português e definição de medidas prospetivas para a oferta desta área curricular nos Ensinos Básico e Secundário”.publishersversionpublishe

New textile for personal protective equipment—plasma chitosan/silver nanoparticles nylon fabric

Fabric structures are prone to contamination with microorganisms, as their morphology and ability to retain moisture creates a proper environment for their growth. In this work, a novel, easily processed and cheap coating for a nylon fabric with antimicrobial characteristics was devel- oped. After plasma treatment, made to render the fabric surface more reactive sites, the fabric was impregnated with chitosan and silver nanoparticles by simply dipping it into a mixture of different concentrations of both components. Silver nanoparticles were previously synthesized using the Lee–Meisel method, and their successful obtention was proven by UV–Vis, showing the presence of the surface plasmon resonance band at 410 nm. Nanoparticles with 25 nm average diameter observed by STEM were stable, mainly in the presence of chitosan, which acted as a surfactant for silver nanoparticles, avoiding their aggregation. The impregnated fabric possessed bactericidal activ- ity higher for Gram-positive Staphylococcus aureus than for Gram-negative Pseudomonas aeruginosa bacteria for all combinations. The percentage of live S. aureus and P. aeruginosa CFU was reduced to less than 20% and 60%, respectively, when exposed to each of the coating combinations. The effect was more pronounced when both chitosan and silver were present in the coating, suggesting an effective synergy between these components. After a washing process, the antimicrobial effect was highly reduced, suggesting that the coating is unstable after washing, being almost completely removed from the fabric. Nevertheless, the new-coated fabric can be successfully used in single-use face masks. To our knowledge, the coating of nylon fabrics intended for face-mask material with both agents has never been reported.This study was supported by the Portuguese Foundation for Science and Technology (FCT), under the scope of the strategic funding of UIDB/04469/2020 unit, and BioTecNorte operation (NORTE-01-0145-FEDER-000004). funded by the European Regional Development, Fund under the scope of Norte2020—Programa Operacional Regional do Norte

Proposta de Orientações Programáticas de Português Língua Não Materna para os Ensinos Básico e Secundário

UID/LIN/03213/2013 PEst-OE/LIN/UI3213/2014Este documento foi elaborado no âmbito do “Estudo de avaliação do impacto da aplicação do Português Língua Não Materna no sistema educativo português e definição de medidas prospetivas para a oferta desta área curricular nos Ensinos Básico e Secundário”.publishersversionpublishe

Protótipos de materiais e recursos para o ensino do Português Língua não Materna nos Ensinos Básico e Secundário

UID/LIN/03213/2013 PEst-OE/LIN/UI3213/2014Este documento foi elaborado no âmbito do “Estudo de avaliação do impacto da aplicação do Português Língua Não Materna no sistema educativo português e definição de medidas prospetivas para a oferta desta área curricular nos Ensinos Básico e Secundário”.publishersversionpublishe

1st Symposium in immunology of sport and physical activity: proceedings book

FCT e Salimetric

Chronic respiratory diseases and quality of life in elderly nursing home residents

Funding The author(s) disclosed receipt of the following financial support for the research, authorship, and/or publication of this article: This study was supported by Fundação para a Ciência e Tecnologia–GERIA Project PTDC/SAU-SAP/ 116563/2010.Few studies have assessed the quality of life (QOL) related to chronic respiratory diseases in the elderly. In the framework of the geriatric study on the health effects of air quality in elderly care centers (GERIA) study, a questionnaire was completed by elderly subjects from 53 selected nursing homes. It included various sections in order to assess respiratory complaints, QOL (World Health Organization QOL (WHOQOL)-BREF), and the cognitive and depression status. The outcome variables were the presence of a score lower than 50 (publishersversionpublishe

Cold atmospheric plasma, a novel approach against bladder cancer, with higher sensitivity for the high-grade cell line

Antitumor therapies based on Cold Atmospheric Plasma (CAP) are an emerging medical field. In this work, we evaluated CAP effects on bladder cancer. Two bladder cancer cell lines were used, HT-1376 (stage III) and TCCSUP (stage IV). Cell proliferation assays were performed evaluating metabolic activity (MTT assay) and protein content (SRB assay). Cell viability, cell cycle, and mitochondrial membrane potential (Δψm) were assessed using flow cytometry. Reactive oxygen and nitrogen species (RONS) and reduced glutathione (GSH) were evaluated by fluorescence. The assays were carried out with different CAP exposure times. For both cell lines, we obtained a significant reduction in metabolic activity and protein content. There was a decrease in cell viability, as well as a cell cycle arrest in S phase. The Δψm was significantly reduced. There was an increase in superoxide and nitric oxide and a decrease in peroxide contents, while GSH content did not change. These results were dependent on the exposure time, with small differences for both cell lines, but overall, they were more pronounced in the TCCSUP cell line. CAP showed to have a promising antitumor effect on bladder cancer, with higher sensitivity for the high-grade cell line.info:eu-repo/semantics/publishedVersio

Blood Pressure Responses in Isokinetic Dynamometry Test in Elderly Community Women: An Exploratory Study

The aim of the study was to verify the systolic, diastolic and mean arterial pressure response in elderly women during isokinetic test. The study included 54 females (68.8 ± 5.9 years) divided into two age groups (Group 1: 60 to 70 and group 2: above 71 years). BP was measured before and immediately after the isokinetic knee extension / flexion test in the concentric-concentric mode. After the test, significant elevations were observed in relation to the pre-test in SBP (G1: Δ% = 29.7%, G2: Δ% = 20.6%, p <.01, respectively) and in MAP (G1: Δ% = 7.6 %, G2: Δ% = 8.4%, p <.01, respectively). The use of isokinetic tests produces elevations in systolic blood pressure and mean arterial pressure in elderly women. Increases occur independently of the age group studied. However, the increases detected do not appear to be of sufficient magnitude to constitute a health hazard whereby isokinetic tests can be safely applied in this population